ButhA.01142.a

50S ribosomal protein L3 (BTH_I3068) (rplC)

CENTER ID: ButhA.01142.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.01142.a.A1.GE32814 full length 1 219
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.01142.a.A1.PW33418 full length 1 219

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.5959
RefSeq: YP_443572.1
UniProt: Q2SU27

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLGLVGRKV GMTRIFTAEG DSIPVTVLDV SDNRVTQIKT VETDGYTAVQ VAFGSRRASR VTKPLAGHLA KAGVQTGEIL KEFRIEADKA AELSNGAVIG PDLFEVGQKV DVQGVSIGKG YAGTIKRYNF GSGRASHGNS RSHNVPGSIG MAQDPGRVFP GKRMTGHMGD ETVTVQNLEI ARIDADRKLL LVKGAVPGAK GGKVFVTPAV KTRAVKGAK
NT Sequence
atgagccttg gactcgtagg tcgcaaggtt ggcatgaccc gtatcttcac ggcggaaggg gactcgatcc ccgtcaccgt gctcgacgtg tcggacaacc gcgtgacgca gatcaagact gttgaaaccg acggctacac ggccgtgcag gttgcattcg gctcccgccg cgcatcgcgc gtgacgaagc cgctggcagg tcatctcgcc aaagccggtg tccaaaccgg tgaaatcctc aaggaattcc gcatcgaagc cgacaaggcc gccgagttgt cgaacggcgc cgtgatcggt ccggatctgt tcgaagtggg ccagaaggtc gacgtgcaag gcgtgtcgat cggtaagggc tacgcgggca cgatcaagcg ctacaacttc ggttccggcc gcgcgtcgca cggtaattcg cgctcgcaca acgtgccggg ctcgatcggt atggcgcagg atccgggccg cgtgtttccg ggcaagcgca tgacgggtca catgggtgac gagacggtga cggttcagaa cctcgaaatc gctcgcatcg acgccgatcg caagctgctg ctcgtcaagg gtgcagttcc gggtgcgaag ggcggcaagg ttttcgtgac gccggccgtg aagacgcgtg ccgtgaaggg ggcgaaataa
Details for ButhA.01142.a.A1.GE32814
HARVESTED ON: 6/28/2011
SEQUENCED ON: 7/22/2011
EXPECTED MW: 25kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSLGLVGRK VGMTRIFTAE GDSIPVTVLD VSDNRVTQIK TVETDGYTAV QVAFGSRRAS RVTKPLAGHL AKAGVQTGEI LKEFRIEADK AAELSNGAVI GPDLFEVGQK VDVQGVSIGK GYAGTIKRYN FGSGRASHGN SRSHNVPGSI GMAQDPGRVF PGKRMTGHMG DETVTVQNLE IARIDADRKL LLVKGAVPGA KGGKVFVTPA VKTRAVKGAK
Validated NT Sequence
ttattatttc gcccccttca cggcacgcgt cttcacggcc ggcgtcacga aaaccttgcc gcccttcgca cccggaactg cacccttgac gagcagcagc ttgcgatcgg cgtcgatgcg agcgatttcg aggttctgaa ccgtcaccgt ctcgtcaccc atgtgacccg tcatgcgctt gcccggaaac acgcggcccg gatcctgcgc cataccgatc gagcccggca cgttgtgcga gcgcgaatta ccgtgcgacg cgcggccgga accgaagttg tagcgcttga tcgtgcccgc gtagccctta ccgatcgaca cgccttgcac gtcgaccttc tggcccactt cgaacagatc cggaccgatc acggcgccgt tcgacaactc ggcggccttg tcggcttcga tgcggaattc cttgaggatt tcaccggttt ggacaccggc tttggcgaga tgacctgcca gcggcttcgt cacgcgcgat gcgcggcggg agccgaatgc aacctgcacg gccgtgtagc cgtcggtttc aacagtcttg atctgcgtca cgcggttgtc cgacacgtcg agcacggtga cggggatcga gtccccttcc gccgtgaaga tacgggtcat gccaaccttg cgacctacga gtccaaggct catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSLGLVGRK VGMTRIFTAE GDSIPVTVLD VSDNRVTQIK TVETDGYTAV QVAFGSRRAS RVTKPLAGHL AKAGVQTGEI LKEFRIEADK AAELSNGAVI GPDLFEVGQK VDVQGVSIGK GYAGTIKRYN FGSGRASHGN SRSHNVPGSI GMAQDPGRVF PGKRMTGHMG DETVTVQNLE IARIDADRKL LLVKGAVPGA KGGKVFVTPA VKTRAVKGAK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagccttgga ctcgtaggtc gcaaggttgg catgacccgt atcttcacgg cggaagggga ctcgatcccc gtcaccgtgc tcgacgtgtc ggacaaccgc gtgacgcaga tcaagactgt tgaaaccgac ggctacacgg ccgtgcaggt tgcattcggc tcccgccgcg catcgcgcgt gacgaagccg ctggcaggtc atctcgccaa agccggtgtc caaaccggtg aaatcctcaa ggaattccgc atcgaagccg acaaggccgc cgagttgtcg aacggcgccg tgatcggtcc ggatctgttc gaagtgggcc agaaggtcga cgtgcaaggc gtgtcgatcg gtaagggcta cgcgggcacg atcaagcgct acaacttcgg ttccggccgc gcgtcgcacg gtaattcgcg ctcgcacaac gtgccgggct cgatcggtat ggcgcaggat ccgggccgcg tgtttccggg caagcgcatg acgggtcaca tgggtgacga gacggtgacg gttcagaacc tcgaaatcgc tcgcatcgac gccgatcgca agctgctgct cgtcaagggt gcagttccgg gtgcgaaggg cggcaaggtt ttcgtgacgc cggccgtgaa gacgcgtgcc gtgaaggggg cgaaataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for ButhA.01142.a.A1.PW33418
PURIFICATION DATe: 10/21/2011
CONCENTRATION: 14.22mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 50µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSLGLVGRK VGMTRIFTAE GDSIPVTVLD VSDNRVTQIK TVETDGYTAV QVAFGSRRAS RVTKPLAGHL AKAGVQTGEI LKEFRIEADK AAELSNGAVI GPDLFEVGQK VDVQGVSIGK GYAGTIKRYN FGSGRASHGN SRSHNVPGSI GMAQDPGRVF PGKRMTGHMG DETVTVQNLE IARIDADRKL LLVKGAVPGA KGGKVFVTPA VKTRAVKGAK
Validated NT Sequence
ttattatttc gcccccttca cggcacgcgt cttcacggcc ggcgtcacga aaaccttgcc gcccttcgca cccggaactg cacccttgac gagcagcagc ttgcgatcgg cgtcgatgcg agcgatttcg aggttctgaa ccgtcaccgt ctcgtcaccc atgtgacccg tcatgcgctt gcccggaaac acgcggcccg gatcctgcgc cataccgatc gagcccggca cgttgtgcga gcgcgaatta ccgtgcgacg cgcggccgga accgaagttg tagcgcttga tcgtgcccgc gtagccctta ccgatcgaca cgccttgcac gtcgaccttc tggcccactt cgaacagatc cggaccgatc acggcgccgt tcgacaactc ggcggccttg tcggcttcga tgcggaattc cttgaggatt tcaccggttt ggacaccggc tttggcgaga tgacctgcca gcggcttcgt cacgcgcgat gcgcggcggg agccgaatgc aacctgcacg gccgtgtagc cgtcggtttc aacagtcttg atctgcgtca cgcggttgtc cgacacgtcg agcacggtga cggggatcga gtccccttcc gccgtgaaga tacgggtcat gccaaccttg cgacctacga gtccaaggct catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSLGLVGRK VGMTRIFTAE GDSIPVTVLD VSDNRVTQIK TVETDGYTAV QVAFGSRRAS RVTKPLAGHL AKAGVQTGEI LKEFRIEADK AAELSNGAVI GPDLFEVGQK VDVQGVSIGK GYAGTIKRYN FGSGRASHGN SRSHNVPGSI GMAQDPGRVF PGKRMTGHMG DETVTVQNLE IARIDADRKL LLVKGAVPGA KGGKVFVTPA VKTRAVKGAK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCCTTGGA CTCGTAGGTC GCAAGGTTGG CATGACCCGT ATCTTCACGG CGGAAGGGGA CTCGATCCCC GTCACCGTGC TCGACGTGTC GGACAACCGC GTGACGCAGA TCAAGACTGT TGAAACCGAC GGCTACACGG CCGTGCAGGT TGCATTCGGC TCCCGCCGCG CATCGCGCGT GACGAAGCCG CTGGCAGGTC ATCTCGCCAA AGCCGGTGTC CAAACCGGTG AAATCCTCAA GGAATTCCGC ATCGAAGCCG ACAAGGCCGC CGAGTTGTCG AACGGCGCCG TGATCGGTCC GGATCTGTTC GAAGTGGGCC AGAAGGTCGA CGTGCAAGGC GTGTCGATCG GTAAGGGCTA CGCGGGCACG ATCAAGCGCT ACAACTTCGG TTCCGGCCGC GCGTCGCACG GTAATTCGCG CTCGCACAAC GTGCCGGGCT CGATCGGTAT GGCGCAGGAT CCGGGCCGCG TGTTTCCGGG CAAGCGCATG ACGGGTCACA TGGGTGACGA GACGGTGACG GTTCAGAACC TCGAAATCGC TCGCATCGAC GCCGATCGCA AGCTGCTGCT CGTCAAGGGT GCAGTTCCGG GTGCGAAGGG CGGCAAGGTT TTCGTGACGC CGGCCGTGAA GACGCGTGCC GTGAAGGGGG CGAAATAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA