ButhA.01136.a

1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXP reductoisomerase) (EC 1.1.1.267) (1-deoxyxylulose-5-phosphate reductoisomerase) (2-C-methyl-D-erythritol 4-phosphate synthase) (BTH_I2033) (dxr)

CENTER ID:	ButhA.01136.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.01136.a.A1.GE32757	full length		1	398

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.01136.a.A1.PW33361	full length		1	398

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_130462
PATRIC ID:	fig\|271848.6.peg.4842
RefSeq:	YP_442557.1
UniProt:	Q2SWZ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQKRLTLLGS TGSIGDSTLD VVTRHPERFA VHALTAHRNG DKLVAQCLRF APDVAVVGDA ETAARVEAQL RAAGSKTQVA YGKQALVDVS KSDGCDTVVA AIVGAAGLAP SLAAARAGKR ILLANKEALV MSGAIFMDAV RDHGAILLPV DSEHNAIFQC MPRDAAEHGG IAKIIVTASG GPFRTREPVT LASVTPDEAC KHPNWVMGRK ISVDSATMMN KGLEVIEAHW LFGLPSERID VLIHPQSVIH SLVSYCDGSV LAQLGNPDMR TPIAHALAFP ERVDAGVAQL DLAQIATLTF EKPDYARFPC LALAIDALEA GGVASAALNA ANEIAVDAFL SRRIRFTAIA QTVDAVLSGL SNRTPSGLDD VIGADADARR AATAFIDKLP APSVERAA

NT Sequence

atgcaaaaac gtctgacatt gctcggttcg acgggctcga tcggagacag cacgctcgat gtcgtcacgc gtcatccgga gcgcttcgcg gttcatgcgc tcacggccca ccgcaacggc gacaagctcg tcgcccagtg cctgcgcttt gcgcccgacg tcgcggtggt cggcgacgcc gaaaccgccg cgcgggtcga ggcgcaattg cgcgccgcgg gcagcaagac ccaggtcgcg tacggcaagc aggcgctcgt cgacgtatcg aagagcgacg gctgcgacac cgtcgtcgcg gcgatcgtcg gcgcggcggg gctcgcgccg agcctcgccg ccgcacgcgc gggcaagcgc atcctgctcg cgaacaagga ggcgctcgtg atgtcgggcg cgatcttcat ggacgcggtg cgcgatcacg gcgcgatcct gttgcccgtc gacagcgagc acaacgcgat cttccagtgc atgccgcgcg acgcggccga gcatggcggg atcgcgaaga tcatcgtgac cgcgtcgggc ggcccgttcc gcacgcgcga gcccgtgacg ctcgcgagcg tcacgcccga cgaggcgtgc aagcatccga actgggtgat gggccgcaag atctccgtcg attccgcgac gatgatgaac aagggcctcg aggtgatcga ggcgcactgg ctgttcggcc tgccgagcga gcgcatcgac gtgttgatcc acccgcagag cgtgatccat tcgctcgtgt catattgcga cggctcggtg ctcgcgcaac tcggcaatcc ggacatgcgc acgccgatcg cgcatgcgct cgcgtttccc gagcgtgtcg acgcgggcgt cgcgcagctc gatctcgcgc agattgcgac gctcacgttc gagaagcccg actacgcgcg ctttccgtgt ctcgcgctcg ccatcgatgc gctcgaggcg ggcggcgtcg cgagcgcggc gctcaatgcg gccaacgaga tcgcggtcga cgcgttcctg tcgcgccgca tccgcttcac cgcgatcgcg cagacggtcg acgcggtgct gagcgggctg tccaatcgta cgccgagcgg cctcgacgac gtgatcggcg cggacgccga cgcgcgccgc gcggcgacgg cgttcatcga caagctgccc gcgcccagcg tcgagcgagc cgcctga

Details for ButhA.01136.a.A1.GE32757

HARVESTED ON:	6/20/2011
SEQUENCED ON:	7/14/2011
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMQKRLTLLG STGSIGDSTL DVVTRHPERF AVHALTAHRN GDKLVAQCLR FAPDVAVVGD AETAARVEAQ LRAAGSKTQV AYGKQALVDV SKSDGCDTVV AAIVGAAGLA PSLAAARAGK RILLANKEAL VMSGAIFMDA VRDHGAILLP VDSEHNAIFQ CMPRDAAEHG GIAKIIVTAS GGPFRTREPV TLASVTPDEA CKHPNWVMGR KISVDSATMM NKGLEVIEAH WLFGLPSERI DVLIHPQSVI HSLVSYCDGS VLAQLGNPDM RTPIAHALAF PERVDAGVAQ LDLAQIATLT FEKPDYARFP CLALAIDALE AGGVASAALN AANEIAVDAF LSRRXRFTAI AQTVDAVLSG LSNRTPSGLD DVIGADADAR RAATAFIDKL PAPSVERAA

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcaaa aacgtctgac attgctcggt tcgacgggct cgatcggaga cagcacgctc gatgtcgtca cgcgtcatcc ggagcgcttc gcggttcatg cgctcacggc ccaccgcaac ggcgacaagc tcgtcgccca gtgcctgcgc tttgcgcccg acgtcgcggt ggtcggcgac gccgaaaccg ccgcgcgggt cgaggcgcaa ttgcgcgccg cgggcagcaa gacccaggtc gcgtacggca agcaggcgct cgtcgacgta tcgaagagcg acggctgcga caccgtcgtc gcggcgatcg tcggcgcggc ggggctcgcg ccgagcctcg ccgccgcacg cgcgggcaag cgcatcctgc tcgcgaacaa ggaggcgctc gtgatgtcgg gcgcgatctt catggacgcg gtgcgcgatc acggcgcgat cctgttgccc gtcgacagcg agcacaacgc gatcttccag tgcatgccgc gcgacgcggc cgagcatggc gggatcgcga agatcatcgt gaccgcgtcg ggcggcccgt tccgcacgcg cgagcccgtg acgctcgcga gcgtcacgcc cgacgaggcg tgcaagcatc cgaactgggt gatgggccgc aagatctccg tcgattccgc gacgatgatg aacaagggcc tcgaggtgat cgaggcgcac tggctgttcg gcctgccgag cgagcgcatc gacgtgttga tccacccgca gagcgtgatc cattcgctcg tgtcatattg cgacggctcg gtgctcgcgc aactcggcaa tccggacatg cgcacgccga tcgcgcatgc gctcgcgttt cccgagcgtg tcgacgcggg cgtcgcgcag ctcgatctcg cgcagattgc gacgctcacg ttcgagaagc ccgactacgc gcgctttccg tgtctcgcgc tcgccatcga tgcgctcgag gcgggcggcg tcgcgagcgc ggcgctcaat gcggccaacg agatcgcggt cgacgcgttc ctgtcgcgcc gcatncgntt caccgcgatc gcgcagacgg tngacgcggt gctgagcggg ctgtccaatc gtacgccgag cggcctcgac gacgtgatcg gcgcggacgc cgacgcgcgc cgcgcggcga cggcgttcat cgacaagctg cccgcgccca gcgtcgagcg agccgcctaa taa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMQKRLTLLG STGSIGDSTL DVVTRHPERF AVHALTAHRN GDKLVAQCLR FAPDVAVVGD AETAARVEAQ LRAAGSKTQV AYGKQALVDV SKSDGCDTVV AAIVGAAGLA PSLAAARAGK RILLANKEAL VMSGAIFMDA VRDHGAILLP VDSEHNAIFQ CMPRDAAEHG GIAKIIVTAS GGPFRTREPV TLASVTPDEA CKHPNWVMGR KISVDSATMM NKGLEVIEAH WLFGLPSERI DVLIHPQSVI HSLVSYCDGS VLAQLGNPDM RTPIAHALAF PERVDAGVAQ LDLAQIATLT FEKPDYARFP CLALAIDALE AGGVASAALN AANEIAVDAF LSRRIRFTAI AQTVDAVLSG LSNRTPSGLD DVIGADADAR RAATAFIDKL PAPSVERAA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaaaaacgt ctgacattgc tcggttcgac gggctcgatc ggagacagca cgctcgatgt cgtcacgcgt catccggagc gcttcgcggt tcatgcgctc acggcccacc gcaacggcga caagctcgtc gcccagtgcc tgcgctttgc gcccgacgtc gcggtggtcg gcgacgccga aaccgccgcg cgggtcgagg cgcaattgcg cgccgcgggc agcaagaccc aggtcgcgta cggcaagcag gcgctcgtcg acgtatcgaa gagcgacggc tgcgacaccg tcgtcgcggc gatcgtcggc gcggcggggc tcgcgccgag cctcgccgcc gcacgcgcgg gcaagcgcat cctgctcgcg aacaaggagg cgctcgtgat gtcgggcgcg atcttcatgg acgcggtgcg cgatcacggc gcgatcctgt tgcccgtcga cagcgagcac aacgcgatct tccagtgcat gccgcgcgac gcggccgagc atggcgggat cgcgaagatc atcgtgaccg cgtcgggcgg cccgttccgc acgcgcgagc ccgtgacgct cgcgagcgtc acgcccgacg aggcgtgcaa gcatccgaac tgggtgatgg gccgcaagat ctccgtcgat tccgcgacga tgatgaacaa gggcctcgag gtgatcgagg cgcactggct gttcggcctg ccgagcgagc gcatcgacgt gttgatccac ccgcagagcg tgatccattc gctcgtgtca tattgcgacg gctcggtgct cgcgcaactc ggcaatccgg acatgcgcac gccgatcgcg catgcgctcg cgtttcccga gcgtgtcgac gcgggcgtcg cgcagctcga tctcgcgcag attgcgacgc tcacgttcga gaagcccgac tacgcgcgct ttccgtgtct cgcgctcgcc atcgatgcgc tcgaggcggg cggcgtcgcg agcgcggcgc tcaatgcggc caacgagatc gcggtcgacg cgttcctgtc gcgccgcatc cgcttcaccg cgatcgcgca gacggtcgac gcggtgctga gcgggctgtc caatcgtacg ccgagcggcc tcgacgacgt gatcggcgcg gacgccgacg cgcgccgcgc ggcgacggcg ttcatcgaca agctgcccgc gcccagcgtc gagcgagccg cctaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for ButhA.01136.a.A1.PW33361

PURIFICATION DATe:	10/28/2011
CONCENTRATION:	25.34mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	50µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMQKRLTLLG STGSIGDSTL DVVTRHPERF AVHALTAHRN GDKLVAQCLR FAPDVAVVGD AETAARVEAQ LRAAGSKTQV AYGKQALVDV SKSDGCDTVV AAIVGAAGLA PSLAAARAGK RILLANKEAL VMSGAIFMDA VRDHGAILLP VDSEHNAIFQ CMPRDAAEHG GIAKIIVTAS GGPFRTREPV TLASVTPDEA CKHPNWVMGR KISVDSATMM NKGLEVIEAH WLFGLPSERI DVLIHPQSVI HSLVSYCDGS VLAQLGNPDM RTPIAHALAF PERVDAGVAQ LDLAQIATLT FEKPDYARFP CLALAIDALE AGGVASAALN AANEIAVDAF LSRRIRFTAI AQTVDAVLSG LSNRTPSGLD DVIGADADAR RAATAFIDKL PAPSVERAA

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAAAAACGT CTGACATTGC TCGGTTCGAC GGGCTCGATC GGAGACAGCA CGCTCGATGT CGTCACGCGT CATCCGGAGC GCTTCGCGGT TCATGCGCTC ACGGCCCACC GCAACGGCGA CAAGCTCGTC GCCCAGTGCC TGCGCTTTGC GCCCGACGTC GCGGTGGTCG GCGACGCCGA AACCGCCGCG CGGGTCGAGG CGCAATTGCG CGCCGCGGGC AGCAAGACCC AGGTCGCGTA CGGCAAGCAG GCGCTCGTCG ACGTATCGAA GAGCGACGGC TGCGACACCG TCGTCGCGGC GATCGTCGGC GCGGCGGGGC TCGCGCCGAG CCTCGCCGCC GCACGCGCGG GCAAGCGCAT CCTGCTCGCG AACAAGGAGG CGCTCGTGAT GTCGGGCGCG ATCTTCATGG ACGCGGTGCG CGATCACGGC GCGATCCTGT TGCCCGTCGA CAGCGAGCAC AACGCGATCT TCCAGTGCAT GCCGCGCGAC GCGGCCGAGC ATGGCGGGAT CGCGAAGATC ATCGTGACCG CGTCGGGCGG CCCGTTCCGC ACGCGCGAGC CCGTGACGCT CGCGAGCGTC ACGCCCGACG AGGCGTGCAA GCATCCGAAC TGGGTGATGG GCCGCAAGAT CTCCGTCGAT TCCGCGACGA TGATGAACAA GGGCCTCGAG GTGATCGAGG CGCACTGGCT GTTCGGCCTG CCGAGCGAGC GCATCGACGT GTTGATCCAC CCGCAGAGCG TGATCCATTC GCTCGTGTCA TATTGCGACG GCTCGGTGCT CGCGCAACTC GGCAATCCGG ACATGCGCAC GCCGATCGCG CATGCGCTCG CGTTTCCCGA GCGTGTCGAC GCGGGCGTCG CGCAGCTCGA TCTCGCGCAG ATTGCGACGC TCACGTTCGA GAAGCCCGAC TACGCGCGCT TTCCGTGTCT CGCGCTCGCC ATCGATGCGC TCGAGGCGGG CGGCGTCGCG AGCGCGGCGC TCAATGCGGC CAACGAGATC GCGGTCGACG CGTTCCTGTC GCGCCGCATC CGCTTCACCG CGATCGCGCA GACGGTCGAC GCGGTGCTGA GCGGGCTGTC CAATCGTACG CCGAGCGGCC TCGACGACGT GATCGGCGCG GACGCCGACG CGCGCCGCGC GGCGACGGCG TTCATCGACA AGCTGCCCGC GCCCAGCGTC GAGCGAGCCG CCTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA