ButhA.01026.a

Glutamate-1-semialdehyde 2,1-aminomutase (GSA) (EC 5.4.3.8) (Glutamate-1-semialdehyde aminotransferase) (GSA-AT) (BTH_I1543) (hemL)

CENTER ID: ButhA.01026.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.01026.a.A1.GE32753 full length 1 427
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.01026.a.A1.PW33357 full length 1 427

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.4325
RefSeq: YP_442087.1
UniProt: Q2SYB2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSNNQTLFER AQRTIPGGVN SPVRAFRSVG GTPRFVARAQ GAYFWDADGK RYIDYIGSWG PMIVGHVHPD VLAAVQRVLA DGFSFGAPTE AEIEIAEEIC KLVPSIEQVR MVSSGTEATM SALRLARGFT GRSRIVKFEG CYHGHADSLL VKAGSGLLTF GNPTSAGVPA DVAKHTTVLE YNNVAALEEA FAAFGDEIAA VIVEPVAGNM NLVRGTPEFL NALRALCTKH GAVLIFDEVM CGFRVALGGA QQHYGIKPDL TCLGKVIGGG MPAAAFGGRG DIMSHLAPLG GVYQAGTLSG NPVAVAAGLA TLRLIQAPGF HDALADKTRR LADGLAAEAR AAGVPFSADA IGGMFGLYFT EQVPASFADV TKSDIERFNR FFHLMLDAGV YFAPSAYEAG FVSSAHDDAT LDATLDAARR AFAALRA
NT Sequence
atgtcgaaca atcaaactct cttcgaacgc gcccagcgaa ccatcccggg cggcgtcaat tcgccggtgc gggcattccg ctcggtcggc ggcacgccgc gcttcgtcgc gcgcgcgcaa ggcgcatact tctgggacgc ggacggcaag cgctacatcg actacatcgg ctcgtggggc ccgatgatcg tcggccacgt gcacccggac gtgctcgccg ccgtgcagcg cgtgctcgcc gacggcttct cgttcggcgc gccgaccgaa gccgaaatcg agatcgccga ggaaatctgc aagctcgtgc cgtcgatcga gcaggtgcgg atggtgtcga gcggcaccga agcgacgatg agcgcgctgc gcctcgcgcg cggcttcacc ggccgcagcc ggatcgtcaa gttcgagggc tgctatcacg gccatgcgga cagcctgctc gtgaaggcgg gctccggcct cctcacgttc ggcaatccga cgtcggccgg cgtgcccgcc gacgtcgcga agcacacgac cgtgctcgag tacaacaacg tcgcggcgct cgaggaagcg ttcgccgcgt tcggcgacga gatcgccgcg gtgatcgtcg agcccgtcgc gggcaacatg aacctcgtgc gcggcacgcc cgaattcctg aacgcgctgc gcgcgctctg cacgaagcac ggcgccgtgc tgatcttcga cgaagtgatg tgcggctttc gcgtcgcgct cggcggcgcg cagcagcact acgggatcaa gccggacctg acctgcctcg gcaaggtgat cggcggcggc atgccggccg ccgcgttcgg cggccgcggc gacatcatgt cgcacctcgc gccgcttggc ggcgtctacc aggccggaac gctgtcgggc aacccggtcg cggtcgcggc gggcctggcg acgctcagac tgatccaggc gccgggcttt cacgacgcgc tcgccgacaa gacccgccgg ctcgccgacg gcctcgcggc cgaggcgcgc gcggcgggcg tgccgttctc ggccgacgcg atcggcggaa tgttcggcct ctacttcacc gagcaggtgc ccgcgagctt cgcagacgtg acgaagagcg acatcgagcg cttcaaccgc ttcttccatc tgatgctcga tgccggcgtg tacttcgcgc cgtccgcgta cgaagcgggc ttcgtgtcga gcgcgcacga cgacgcgacg ctcgacgcga cgctcgacgc cgcgcgccgc gcgttcgcgg cgctgcgcgc ctga
Details for ButhA.01026.a.A1.GE32753
HARVESTED ON: 6/20/2011
SEQUENCED ON: 7/14/2011
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSNNQTLFE RAQRTIPGGV NSPVRAFRSV GGTPRFVARA QGAYFWDADG KRYIDYIGSW GPMIVGHVHP DVLAAVQRVL ADGFSFGAPT EAEIEIAEEI CKLVPSIEQV RMVSSGTEAT MSALRLARGF TGRSRIVKFE GCYHGHADSL LVKAGSGLLT FGNPTSAGVP ADVAKHTTVL EYNNVAALEE AFAAFGDEIA AVIVEPVAGN MNLVRGTPEF LNALRALCTK HGAVLIFDEV MCGFRVALGG AQQHYGIKPD LTCLGKVIGG GMPAAAFGGR GDIMSHLAPL GGVYQAGTLS GNPVAVAAGL ATLRLIQAPG FHDALADKTR RLADGLAAEA RAAGVPFSAD AIGGMFGLYF TEQVPASFAD VTKSDIERFN RFFHLMLDAG VYFAPSAYEA GFVSSAHDDA TLDATLDAAR RAFAAL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcga acaatcaaac tctcttcgaa cgcgcccagc gaaccatccc gggcggcgtc aattcgccgg tgcgggcatt ccgctcggtc ggcggcacgc cgcgcttcgt cgcgcgcgcg caaggcgcat acttctggga cgcggacggc aagcgctaca tcgactacat cggctcgtgg ggcccgatga tcgtcggcca cgtgcacccg gacgtgctcg ccgccgtgca gcgcgtgctc gccgacggct tctcgttcgg cgcgccgacc gaagccgaaa tcgagatcgc cgaggaaatc tgcaagctcg tgccgtcgat cgagcaggtg cggatggtgt cgagcggcac cgaagcgacg atgagcgcgc tgcgcctcgc gcgcggcttc accggccgca gccggatcgt caagttcgag ggctgctatc acggccatgc ggacagcctg ctcgtgaagg cgggctccgg cctcctcacg ttcggcaatc cgacgtcggc cggcgtgccc gccgacgtcg cgaagcacac gaccgtgctc gagtacaaca acgtcgcggc gctcgaggaa gcgttcgccg cgttcggcga cgagatcgcc gcggtgatcg tcgagcccgt cgcgggcaac atgaacctcg tgcgcggcac gcccgaattc ctgaacgcgc tgcgcgcgct ctgcacgaag cacggcgccg tgctgatctt cgacgaagtg atgtgcggct ttcgcgtcgc gctcggcggc gcgcagcagc actacgggat caagccggac ctgacctgcc tcggcaaggt gatcggcggc ggcatgccgg ccgccgcgtt cggcggccgc ggcgacatca tgtcgcacct cgcgccgctt ggcggcgtct accaggccgg aacgctgtcg ggcaacccgg tcgcggtcgc ggcgggcctg gcgacgctca gactgatcca ggcgccgggc tttcacgacg cgctcgccga caagacccgc cggctcgccg acggcctcgc ggccgaggcg cgcgcggcgg gcgtgccgtt ctcggccgac gcgatcggcg gaatgttcgg cctctacttc accgagcagg tgcccgcgag cttcgcagac gtgacgaaga gcgacatcga gcgcttcaac cgcttcttcc atctgatgct cgatgccggc gtgtacttcg cgccgtccgc gtacgaagcg ggcttcgtgt cgagcgcgca cgacgacgcg acgctcgacg cgacgctcga cgccgcgcgc cgcgcgttcg cggcgctgcg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSNNQTLFE RAQRTIPGGV NSPVRAFRSV GGTPRFVARA QGAYFWDADG KRYIDYIGSW GPMIVGHVHP DVLAAVQRVL ADGFSFGAPT EAEIEIAEEI CKLVPSIEQV RMVSSGTEAT MSALRLARGF TGRSRIVKFE GCYHGHADSL LVKAGSGLLT FGNPTSAGVP ADVAKHTTVL EYNNVAALEE AFAAFGDEIA AVIVEPVAGN MNLVRGTPEF LNALRALCTK HGAVLIFDEV MCGFRVALGG AQQHYGIKPD LTCLGKVIGG GMPAAAFGGR GDIMSHLAPL GGVYQAGTLS GNPVAVAAGL ATLRLIQAPG FHDALADKTR RLADGLAAEA RAAGVPFSAD AIGGMFGLYF TEQVPASFAD VTKSDIERFN RFFHLMLDAG VYFAPSAYEA GFVSSAHDDA TLDATLDAAR RAFAALRA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgaacaat caaactctct tcgaacgcgc ccagcgaacc atcccgggcg gcgtcaattc gccggtgcgg gcattccgct cggtcggcgg cacgccgcgc ttcgtcgcgc gcgcgcaagg cgcatacttc tgggacgcgg acggcaagcg ctacatcgac tacatcggct cgtggggccc gatgatcgtc ggccacgtgc acccggacgt gctcgccgcc gtgcagcgcg tgctcgccga cggcttctcg ttcggcgcgc cgaccgaagc cgaaatcgag atcgccgagg aaatctgcaa gctcgtgccg tcgatcgagc aggtgcggat ggtgtcgagc ggcaccgaag cgacgatgag cgcgctgcgc ctcgcgcgcg gcttcaccgg ccgcagccgg atcgtcaagt tcgagggctg ctatcacggc catgcggaca gcctgctcgt gaaggcgggc tccggcctcc tcacgttcgg caatccgacg tcggccggcg tgcccgccga cgtcgcgaag cacacgaccg tgctcgagta caacaacgtc gcggcgctcg aggaagcgtt cgccgcgttc ggcgacgaga tcgccgcggt gatcgtcgag cccgtcgcgg gcaacatgaa cctcgtgcgc ggcacgcccg aattcctgaa cgcgctgcgc gcgctctgca cgaagcacgg cgccgtgctg atcttcgacg aagtgatgtg cggctttcgc gtcgcgctcg gcggcgcgca gcagcactac gggatcaagc cggacctgac ctgcctcggc aaggtgatcg gcggcggcat gccggccgcc gcgttcggcg gccgcggcga catcatgtcg cacctcgcgc cgcttggcgg cgtctaccag gccggaacgc tgtcgggcaa cccggtcgcg gtcgcggcgg gcctggcgac gctcagactg atccaggcgc cgggctttca cgacgcgctc gccgacaaga cccgccggct cgccgacggc ctcgcggccg aggcgcgcgc ggcgggcgtg ccgttctcgg ccgacgcgat cggcggaatg ttcggcctct acttcaccga gcaggtgccc gcgagcttcg cagacgtgac gaagagcgac atcgagcgct tcaaccgctt cttccatctg atgctcgatg ccggcgtgta cttcgcgccg tccgcgtacg aagcgggctt cgtgtcgagc gcgcacgacg acgcgacgct cgacgcgacg ctcgacgccg cgcgccgcgc gttcgcggcg ctgcgcgcct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for ButhA.01026.a.A1.PW33357
PURIFICATION DATe: 8/13/2011
CONCENTRATION: 44.51mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 16
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSNNQTLFE RAQRTIPGGV NSPVRAFRSV GGTPRFVARA QGAYFWDADG KRYIDYIGSW GPMIVGHVHP DVLAAVQRVL ADGFSFGAPT EAEIEIAEEI CKLVPSIEQV RMVSSGTEAT MSALRLARGF TGRSRIVKFE GCYHGHADSL LVKAGSGLLT FGNPTSAGVP ADVAKHTTVL EYNNVAALEE AFAAFGDEIA AVIVEPVAGN MNLVRGTPEF LNALRALCTK HGAVLIFDEV MCGFRVALGG AQQHYGIKPD LTCLGKVIGG GMPAAAFGGR GDIMSHLAPL GGVYQAGTLS GNPVAVAAGL ATLRLIQAPG FHDALADKTR RLADGLAAEA RAAGVPFSAD AIGGMFGLYF TEQVPASFAD VTKSDIERFN RFFHLMLDAG VYFAPSAYEA GFVSSAHDDA TLDATLDAAR RAFAAL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcga acaatcaaac tctcttcgaa cgcgcccagc gaaccatccc gggcggcgtc aattcgccgg tgcgggcatt ccgctcggtc ggcggcacgc cgcgcttcgt cgcgcgcgcg caaggcgcat acttctggga cgcggacggc aagcgctaca tcgactacat cggctcgtgg ggcccgatga tcgtcggcca cgtgcacccg gacgtgctcg ccgccgtgca gcgcgtgctc gccgacggct tctcgttcgg cgcgccgacc gaagccgaaa tcgagatcgc cgaggaaatc tgcaagctcg tgccgtcgat cgagcaggtg cggatggtgt cgagcggcac cgaagcgacg atgagcgcgc tgcgcctcgc gcgcggcttc accggccgca gccggatcgt caagttcgag ggctgctatc acggccatgc ggacagcctg ctcgtgaagg cgggctccgg cctcctcacg ttcggcaatc cgacgtcggc cggcgtgccc gccgacgtcg cgaagcacac gaccgtgctc gagtacaaca acgtcgcggc gctcgaggaa gcgttcgccg cgttcggcga cgagatcgcc gcggtgatcg tcgagcccgt cgcgggcaac atgaacctcg tgcgcggcac gcccgaattc ctgaacgcgc tgcgcgcgct ctgcacgaag cacggcgccg tgctgatctt cgacgaagtg atgtgcggct ttcgcgtcgc gctcggcggc gcgcagcagc actacgggat caagccggac ctgacctgcc tcggcaaggt gatcggcggc ggcatgccgg ccgccgcgtt cggcggccgc ggcgacatca tgtcgcacct cgcgccgctt ggcggcgtct accaggccgg aacgctgtcg ggcaacccgg tcgcggtcgc ggcgggcctg gcgacgctca gactgatcca ggcgccgggc tttcacgacg cgctcgccga caagacccgc cggctcgccg acggcctcgc ggccgaggcg cgcgcggcgg gcgtgccgtt ctcggccgac gcgatcggcg gaatgttcgg cctctacttc accgagcagg tgcccgcgag cttcgcagac gtgacgaaga gcgacatcga gcgcttcaac cgcttcttcc atctgatgct cgatgccggc gtgtacttcg cgccgtccgc gtacgaagcg ggcttcgtgt cgagcgcgca cgacgacgcg acgctcgacg cgacgctcga cgccgcgcgc cgcgcgttcg cggcgctgcg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSNNQTLFE RAQRTIPGGV NSPVRAFRSV GGTPRFVARA QGAYFWDADG KRYIDYIGSW GPMIVGHVHP DVLAAVQRVL ADGFSFGAPT EAEIEIAEEI CKLVPSIEQV RMVSSGTEAT MSALRLARGF TGRSRIVKFE GCYHGHADSL LVKAGSGLLT FGNPTSAGVP ADVAKHTTVL EYNNVAALEE AFAAFGDEIA AVIVEPVAGN MNLVRGTPEF LNALRALCTK HGAVLIFDEV MCGFRVALGG AQQHYGIKPD LTCLGKVIGG GMPAAAFGGR GDIMSHLAPL GGVYQAGTLS GNPVAVAAGL ATLRLIQAPG FHDALADKTR RLADGLAAEA RAAGVPFSAD AIGGMFGLYF TEQVPASFAD VTKSDIERFN RFFHLMLDAG VYFAPSAYEA GFVSSAHDDA TLDATLDAAR RAFAALRA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCGAACAAT CAAACTCTCT TCGAACGCGC CCAGCGAACC ATCCCGGGCG GCGTCAATTC GCCGGTGCGG GCATTCCGCT CGGTCGGCGG CACGCCGCGC TTCGTCGCGC GCGCGCAAGG CGCATACTTC TGGGACGCGG ACGGCAAGCG CTACATCGAC TACATCGGCT CGTGGGGCCC GATGATCGTC GGCCACGTGC ACCCGGACGT GCTCGCCGCC GTGCAGCGCG TGCTCGCCGA CGGCTTCTCG TTCGGCGCGC CGACCGAAGC CGAAATCGAG ATCGCCGAGG AAATCTGCAA GCTCGTGCCG TCGATCGAGC AGGTGCGGAT GGTGTCGAGC GGCACCGAAG CGACGATGAG CGCGCTGCGC CTCGCGCGCG GCTTCACCGG CCGCAGCCGG ATCGTCAAGT TCGAGGGCTG CTATCACGGC CATGCGGACA GCCTGCTCGT GAAGGCGGGC TCCGGCCTCC TCACGTTCGG CAATCCGACG TCGGCCGGCG TGCCCGCCGA CGTCGCGAAG CACACGACCG TGCTCGAGTA CAACAACGTC GCGGCGCTCG AGGAAGCGTT CGCCGCGTTC GGCGACGAGA TCGCCGCGGT GATCGTCGAG CCCGTCGCGG GCAACATGAA CCTCGTGCGC GGCACGCCCG AATTCCTGAA CGCGCTGCGC GCGCTCTGCA CGAAGCACGG CGCCGTGCTG ATCTTCGACG AAGTGATGTG CGGCTTTCGC GTCGCGCTCG GCGGCGCGCA GCAGCACTAC GGGATCAAGC CGGACCTGAC CTGCCTCGGC AAGGTGATCG GCGGCGGCAT GCCGGCCGCC GCGTTCGGCG GCCGCGGCGA CATCATGTCG CACCTCGCGC CGCTTGGCGG CGTCTACCAG GCCGGAACGC TGTCGGGCAA CCCGGTCGCG GTCGCGGCGG GCCTGGCGAC GCTCAGACTG ATCCAGGCGC CGGGCTTTCA CGACGCGCTC GCCGACAAGA CCCGCCGGCT CGCCGACGGC CTCGCGGCCG AGGCGCGCGC GGCGGGCGTG CCGTTCTCGG CCGACGCGAT CGGCGGAATG TTCGGCCTCT ACTTCACCGA GCAGGTGCCC GCGAGCTTCG CAGACGTGAC GAAGAGCGAC ATCGAGCGCT TCAACCGCTT CTTCCATCTG ATGCTCGATG CCGGCGTGTA CTTCGCGCCG TCCGCGTACG AAGCGGGCTT CGTGTCGAGC GCGCACGACG ACGCGACGCT CGACGCGACG CTCGACGCCG CGCGCCGCGC GTTCGCGGCG CTGCGCGCCT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA