ButhA.01012.a

Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B (Asp/Glu-ADT subunit B) (EC 6.3.5.-) (BTH_I0149) (gatB)

CENTER ID: ButhA.01012.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.01012.a.A1.GE32811 full length 1 490
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.01012.a.A1.PS01237 full length 1 490

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.2793
RefSeq: YP_440707.1
UniProt: Q2T292

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQWEVVIGL ETHAQLSTVS KIFSGASTQF GAEPNTQACP VDLALPGVLP VLNRGAVERA IRFGLAIGAT VAPRSIFARK NYFYPDLPKG YQISQYEIPV VQGGQITIQV PANEKAGKQA YSKTVNLTRA HLEEDAGKSL HEDFAGMTGI DLNRAGTPLL EIVTEPEMRS AAEAVAYAKA LHGLVVWLGI CDGNMQEGSF RCDANVSVRP VGQEKFGTRA EIKNLNSFRF LEDAINYEVR RQIELIEDGG EVVQETRLYD PDKRETRSMR SKEDAHDYRY FPDPDLMPLV IGADWIERVK GEMPELPAAM QQRFVEQYGV SAYDAGVLTS TKAMAAYFEA VVAKAGAANA KIAANWLMGD VSSQLNRDGI DIDACPVSAA QLALVLQRIA DGTISNKIAK EIFVAIWDEK AADEGAADRI IDAKGLKQIS DTGALEAIID EVLAANAKSV EEFRAGKEKA FNALVGQAMK ATKGKANPQQ VNELLKKKLG
NT Sequence
atgacccagt gggaagtcgt tatcggcctc gagacgcacg cgcaactgtc gaccgtctcg aagattttct cgggcgcgtc gacgcagttc ggcgcggaac ccaacacgca ggcgtgcccc gtcgatctcg cgctgccggg cgtgctgccc gtgctgaacc gcggcgcggt cgagcgggcg atccgcttcg gcctcgcgat cggcgcgacg gtggcgccgc gcagcatctt cgcgcgcaag aattacttct atccggatct gccgaagggc tatcagatca gccagtacga gattccggtc gtgcagggcg gccagatcac gatccaggtg cccgccaacg aaaaggcagg caagcaggcg tattcgaaga cggtcaacct gacccgcgcg cacctcgaag aggacgcggg caaatcgctg cacgaggact tcgcggggat gacgggcatc gacctgaacc gcgcgggcac gccgctgctc gagatcgtca ccgagccgga aatgaggagc gcggccgagg cggtcgcgta cgcgaaggcg ctgcacggcc tcgtcgtgtg gctcggcatc tgcgacggca acatgcagga aggatcgttc cgctgcgacg cgaacgtgtc ggtgcgcccg gtcggccagg agaagttcgg cacgcgcgcc gagatcaaga acctgaactc gttccgcttc ctcgaggacg cgatcaacta tgaagtgcgc cgccagatcg agctgatcga ggacggcggc gaagtcgtgc aggaaacgcg cctgtacgat ccggacaagc gcgagacgcg ctcgatgcgc agcaaggaag acgcgcacga ctaccgctac ttccccgatc cggacctgat gccgctcgtg atcggcgcgg actggatcga gcgcgtgaag ggcgagatgc ccgagctgcc ggccgcgatg cagcagcgct tcgtcgagca gtacggcgtg tccgcgtacg acgcgggcgt gctgacgtcg acgaaggcga tggccgcgta cttcgaggcc gtcgtcgcga aggcgggcgc ggcaaacgcg aagatcgccg cgaactggct gatgggcgac gtgtcgtcgc aactcaatcg cgacggcatc gacatcgatg cgtgcccggt gtcggccgcg cagctcgcgc tcgtgctgca gcggatcgcc gacggcacga tctcgaacaa gatcgcgaag gaaatcttcg tggcgatctg ggacgagaag gcggccgacg aaggcgcggc cgaccgcatc atcgatgcga agggcctgaa gcagatctcc gatacgggcg cgctcgaggc gatcatcgac gaggtgctcg ccgcgaacgc gaagtcggtc gaggagttcc gcgcgggcaa ggaaaaggcg ttcaacgcgc tcgtcggcca ggcgatgaag gcgacgaagg gcaaggcgaa cccgcagcag gtcaacgagc tcctgaagaa gaagctcggc tga
Details for ButhA.01012.a.A1.GE32811
HARVESTED ON: 6/28/2011
SEQUENCED ON: 7/22/2011
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 95
Validated AA Sequence
MTQWEVVIGL ETHAQLSTVS KIFSGASTQF GAEPNTQACP VDLALPGVLP VLNRGAVERA IRFGLAIGAT VAPRSIFARK NYFYPDLPKG YQISQYEIPV VQGGQITIQV PANEKAGKQA YSKTVNLTRA HLEEDAGKSL HEDFAGMTGI DLNRAGTPLL EIVTEPEMRS AAXAVAYAKA LHGLVVWLGI CDGNMQEGSF RCDANVSVRP VGQEKFGTRA EIKNLNSFRF LEDAINYEVR RQIELIEDGG EVVQETRLYD PDKRETRSMR SKEDAHDYRY FPDPDLMPLV IGADWIERVK GEMPELPAAM QQRFVEQYGV SAYDAGVLTS TKAMAAYFEA VVAKAGAANA KIAANWLMGD VSSQLNRDGI DIDACPVSAA QLALVLQRIA DGTISNKIAK EIFVAIWDEK AADEGAADRI IDAKGLKQIS DTGALEAIID EVLAANAKSV EEFRAGKEKA FNALVGQAMK ATKGKANPQQ VNELLKKKLG
Validated NT Sequence
ttattagccg agcttcttct tcaggagctc gttgacctgc tgcgggttcg ccttgccctt cgtcgccttc atcgcctggc cgacgagcgc gttgaacgcc ttttccttgc ccgcgcggaa ctcctcgacc gacttcgcgt tcgcggcgag cacctcgtcg atgatcgcct cgagcgcgcc cgtatcggag atctgcttca ggcccttcgc atcgatgatg cggtcggccg cgccttcgtc ggccgccttc tcgtcccaga tcgccacgaa gatttccttc gcgatcttgt tcgagatcgt gccgtcggcg atccgctgca gcacgagcgc gagctgcgcg gccgacaccg ggcacgcatc gatgtcgatg ccgtcgcgat tgagttgcga cgacacgtcg cccatcagcc agttcgcggc gatcttcgcg tttgccgcgc ccgccttcgc gacgacggcc tcgaagtacg cggccatcgc cttcgtcgac gtcagcacgc ccgcgtcgta cgcggacacg ccgtactgct cgacgaagcg ctgctgcatc gcggccggca gctcgggcat ctcgcccttc acgcgctcga tccagtccgc gccgatcacg agcggcatca ggtccggatc ggggaagtag cggtagtcgt gcgcgtcttc cttgctgcgc atcgagcgcg tctcgcgctt gtccggatcg tacaggcgcg tttcctgcac gacttcgccg ccgtcctcga tcagctcgat ctggcggcgc acttcatagt tgatcgcgtc ctcgaggaag cggaacgagt tcaggttctt gatctcggcg cgcgtgccga acttctcctg nccgaccggg cgcaccgaca cgttcgcgtc gcagcggaac gatccttcct gcatgttgcc gtcgcagatg ccgagccaca cgacgaggcc gtgcagcgcc ttcgcgtacg cgaccgcntc ggccgcgctc ctcatttccg gctcggtgac gatctcgagc agcggcgtgc ccgcgcggtt caggtcgatg cccgtcatcc ccgcgaagtc ctcgtgcagc gatttgcccg cgtcctcttc gaggtgcgcg cgggtcaggt tgaccgtctt cgaatacgcc tgcttgcctg ccttttcgtt ggcgggcacc tggatcgtga tctggccgcc ctgcacgacc ggaatctcgt actggctgat ctgatagccc ttcggcagat ccggatagaa gtaattcttg cgcgcgaaga tgctgcgcgg cgccaccgtc gcgccgatcg cgaggccgaa gcggatcgcc cgctcgaccg cgccgcggtt cagcacgggc agcacgcccg gcagcgcgag atcgacgggg cacgcctgcg tgttgggttc cgcgccgaac tgcgtcgacg cgcccgagaa aatcttcgag acggtcgaca gttgcgcgtg cgtctcgagg ccgataacga cttcccactg ggtcatcgaa ccaggaccct gggtctgagc ttccagg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTQWEVVIG LETHAQLSTV SKIFSGASTQ FGAEPNTQAC PVDLALPGVL PVLNRGAVER AIRFGLAIGA TVAPRSIFAR KNYFYPDLPK GYQISQYEIP VVQGGQITIQ VPANEKAGKQ AYSKTVNLTR AHLEEDAGKS LHEDFAGMTG IDLNRAGTPL LEIVTEPEMR SAAEAVAYAK ALHGLVVWLG ICDGNMQEGS FRCDANVSVR PVGQEKFGTR AEIKNLNSFR FLEDAINYEV RRQIELIEDG GEVVQETRLY DPDKRETRSM RSKEDAHDYR YFPDPDLMPL VIGADWIERV KGEMPELPAA MQQRFVEQYG VSAYDAGVLT STKAMAAYFE AVVAKAGAAN AKIAANWLMG DVSSQLNRDG IDIDACPVSA AQLALVLQRI ADGTISNKIA KEIFVAIWDE KAADEGAADR IIDAKGLKQI SDTGALEAII DEVLAANAKS VEEFRAGKEK AFNALVGQAM KATKGKANPQ QVNELLKKKL G
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacccagtgg gaagtcgtta tcggcctcga gacgcacgcg caactgtcga ccgtctcgaa gattttctcg ggcgcgtcga cgcagttcgg cgcggaaccc aacacgcagg cgtgccccgt cgatctcgcg ctgccgggcg tgctgcccgt gctgaaccgc ggcgcggtcg agcgggcgat ccgcttcggc ctcgcgatcg gcgcgacggt ggcgccgcgc agcatcttcg cgcgcaagaa ttacttctat ccggatctgc cgaagggcta tcagatcagc cagtacgaga ttccggtcgt gcagggcggc cagatcacga tccaggtgcc cgccaacgaa aaggcaggca agcaggcgta ttcgaagacg gtcaacctga cccgcgcgca cctcgaagag gacgcgggca aatcgctgca cgaggacttc gcggggatga cgggcatcga cctgaaccgc gcgggcacgc cgctgctcga gatcgtcacc gagccggaaa tgaggagcgc ggccgaggcg gtcgcgtacg cgaaggcgct gcacggcctc gtcgtgtggc tcggcatctg cgacggcaac atgcaggaag gatcgttccg ctgcgacgcg aacgtgtcgg tgcgcccggt cggccaggag aagttcggca cgcgcgccga gatcaagaac ctgaactcgt tccgcttcct cgaggacgcg atcaactatg aagtgcgccg ccagatcgag ctgatcgagg acggcggcga agtcgtgcag gaaacgcgcc tgtacgatcc ggacaagcgc gagacgcgct cgatgcgcag caaggaagac gcgcacgact accgctactt ccccgatccg gacctgatgc cgctcgtgat cggcgcggac tggatcgagc gcgtgaaggg cgagatgccc gagctgccgg ccgcgatgca gcagcgcttc gtcgagcagt acggcgtgtc cgcgtacgac gcgggcgtgc tgacgtcgac gaaggcgatg gccgcgtact tcgaggccgt cgtcgcgaag gcgggcgcgg caaacgcgaa gatcgccgcg aactggctga tgggcgacgt gtcgtcgcaa ctcaatcgcg acggcatcga catcgatgcg tgcccggtgt cggccgcgca gctcgcgctc gtgctgcagc ggatcgccga cggcacgatc tcgaacaaga tcgcgaagga aatcttcgtg gcgatctggg acgagaaggc ggccgacgaa ggcgcggccg accgcatcat cgatgcgaag ggcctgaagc agatctccga tacgggcgcg ctcgaggcga tcatcgacga ggtgctcgcc gcgaacgcga agtcggtcga ggagttccgc gcgggcaagg aaaaggcgtt caacgcgctc gtcggccagg cgatgaaggc gacgaagggc aaggcgaacc cgcagcaggt caacgagctc ctgaagaaga agctcggcta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for ButhA.01012.a.A1.PS01237
PURIFICATION DATe: 9/27/2011
CONCENTRATION: 40.1mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MTQWEVVIGL ETHAQLSTVS KIFSGASTQF GAEPNTQACP VDLALPGVLP VLNRGAVERA IRFGLAIGAT VAPRSIFARK NYFYPDLPKG YQISQYEIPV VQGGQITIQV PANEKAGKQA YSKTVNLTRA HLEEDAGKSL HEDFAGMTGI DLNRAGTPLL EIVTEPEMRS AAXAVAYAKA LHGLVVWLGI CDGNMQEGSF RCDANVSVRP VGQEKFGTRA EIKNLNSFRF LEDAINYEVR RQIELIEDGG EVVQETRLYD PDKRETRSMR SKEDAHDYRY FPDPDLMPLV IGADWIERVK GEMPELPAAM QQRFVEQYGV SAYDAGVLTS TKAMAAYFEA VVAKAGAANA KIAANWLMGD VSSQLNRDGI DIDACPVSAA QLALVLQRIA DGTISNKIAK EIFVAIWDEK AADEGAADRI IDAKGLKQIS DTGALEAIID EVLAANAKSV EEFRAGKEKA FNALVGQAMK ATKGKANPQQ VNELLKKKLG
Validated NT Sequence
ttattagccg agcttcttct tcaggagctc gttgacctgc tgcgggttcg ccttgccctt cgtcgccttc atcgcctggc cgacgagcgc gttgaacgcc ttttccttgc ccgcgcggaa ctcctcgacc gacttcgcgt tcgcggcgag cacctcgtcg atgatcgcct cgagcgcgcc cgtatcggag atctgcttca ggcccttcgc atcgatgatg cggtcggccg cgccttcgtc ggccgccttc tcgtcccaga tcgccacgaa gatttccttc gcgatcttgt tcgagatcgt gccgtcggcg atccgctgca gcacgagcgc gagctgcgcg gccgacaccg ggcacgcatc gatgtcgatg ccgtcgcgat tgagttgcga cgacacgtcg cccatcagcc agttcgcggc gatcttcgcg tttgccgcgc ccgccttcgc gacgacggcc tcgaagtacg cggccatcgc cttcgtcgac gtcagcacgc ccgcgtcgta cgcggacacg ccgtactgct cgacgaagcg ctgctgcatc gcggccggca gctcgggcat ctcgcccttc acgcgctcga tccagtccgc gccgatcacg agcggcatca ggtccggatc ggggaagtag cggtagtcgt gcgcgtcttc cttgctgcgc atcgagcgcg tctcgcgctt gtccggatcg tacaggcgcg tttcctgcac gacttcgccg ccgtcctcga tcagctcgat ctggcggcgc acttcatagt tgatcgcgtc ctcgaggaag cggaacgagt tcaggttctt gatctcggcg cgcgtgccga acttctcctg nccgaccggg cgcaccgaca cgttcgcgtc gcagcggaac gatccttcct gcatgttgcc gtcgcagatg ccgagccaca cgacgaggcc gtgcagcgcc ttcgcgtacg cgaccgcntc ggccgcgctc ctcatttccg gctcggtgac gatctcgagc agcggcgtgc ccgcgcggtt caggtcgatg cccgtcatcc ccgcgaagtc ctcgtgcagc gatttgcccg cgtcctcttc gaggtgcgcg cgggtcaggt tgaccgtctt cgaatacgcc tgcttgcctg ccttttcgtt ggcgggcacc tggatcgtga tctggccgcc ctgcacgacc ggaatctcgt actggctgat ctgatagccc ttcggcagat ccggatagaa gtaattcttg cgcgcgaaga tgctgcgcgg cgccaccgtc gcgccgatcg cgaggccgaa gcggatcgcc cgctcgaccg cgccgcggtt cagcacgggc agcacgcccg gcagcgcgag atcgacgggg cacgcctgcg tgttgggttc cgcgccgaac tgcgtcgacg cgcccgagaa aatcttcgag acggtcgaca gttgcgcgtg cgtctcgagg ccgataacga cttcccactg ggtcatcgaa ccaggaccct gggtctgagc ttccagg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTQWEVVIG LETHAQLSTV SKIFSGASTQ FGAEPNTQAC PVDLALPGVL PVLNRGAVER AIRFGLAIGA TVAPRSIFAR KNYFYPDLPK GYQISQYEIP VVQGGQITIQ VPANEKAGKQ AYSKTVNLTR AHLEEDAGKS LHEDFAGMTG IDLNRAGTPL LEIVTEPEMR SAAEAVAYAK ALHGLVVWLG ICDGNMQEGS FRCDANVSVR PVGQEKFGTR AEIKNLNSFR FLEDAINYEV RRQIELIEDG GEVVQETRLY DPDKRETRSM RSKEDAHDYR YFPDPDLMPL VIGADWIERV KGEMPELPAA MQQRFVEQYG VSAYDAGVLT STKAMAAYFE AVVAKAGAAN AKIAANWLMG DVSSQLNRDG IDIDACPVSA AQLALVLQRI ADGTISNKIA KEIFVAIWDE KAADEGAADR IIDAKGLKQI SDTGALEAII DEVLAANAKS VEEFRAGKEK AFNALVGQAM KATKGKANPQ QVNELLKKKL G
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCCAGTGG GAAGTCGTTA TCGGCCTCGA GACGCACGCG CAACTGTCGA CCGTCTCGAA GATTTTCTCG GGCGCGTCGA CGCAGTTCGG CGCGGAACCC AACACGCAGG CGTGCCCCGT CGATCTCGCG CTGCCGGGCG TGCTGCCCGT GCTGAACCGC GGCGCGGTCG AGCGGGCGAT CCGCTTCGGC CTCGCGATCG GCGCGACGGT GGCGCCGCGC AGCATCTTCG CGCGCAAGAA TTACTTCTAT CCGGATCTGC CGAAGGGCTA TCAGATCAGC CAGTACGAGA TTCCGGTCGT GCAGGGCGGC CAGATCACGA TCCAGGTGCC CGCCAACGAA AAGGCAGGCA AGCAGGCGTA TTCGAAGACG GTCAACCTGA CCCGCGCGCA CCTCGAAGAG GACGCGGGCA AATCGCTGCA CGAGGACTTC GCGGGGATGA CGGGCATCGA CCTGAACCGC GCGGGCACGC CGCTGCTCGA GATCGTCACC GAGCCGGAAA TGAGGAGCGC GGCCGAGGCG GTCGCGTACG CGAAGGCGCT GCACGGCCTC GTCGTGTGGC TCGGCATCTG CGACGGCAAC ATGCAGGAAG GATCGTTCCG CTGCGACGCG AACGTGTCGG TGCGCCCGGT CGGCCAGGAG AAGTTCGGCA CGCGCGCCGA GATCAAGAAC CTGAACTCGT TCCGCTTCCT CGAGGACGCG ATCAACTATG AAGTGCGCCG CCAGATCGAG CTGATCGAGG ACGGCGGCGA AGTCGTGCAG GAAACGCGCC TGTACGATCC GGACAAGCGC GAGACGCGCT CGATGCGCAG CAAGGAAGAC GCGCACGACT ACCGCTACTT CCCCGATCCG GACCTGATGC CGCTCGTGAT CGGCGCGGAC TGGATCGAGC GCGTGAAGGG CGAGATGCCC GAGCTGCCGG CCGCGATGCA GCAGCGCTTC GTCGAGCAGT ACGGCGTGTC CGCGTACGAC GCGGGCGTGC TGACGTCGAC GAAGGCGATG GCCGCGTACT TCGAGGCCGT CGTCGCGAAG GCGGGCGCGG CAAACGCGAA GATCGCCGCG AACTGGCTGA TGGGCGACGT GTCGTCGCAA CTCAATCGCG ACGGCATCGA CATCGATGCG TGCCCGGTGT CGGCCGCGCA GCTCGCGCTC GTGCTGCAGC GGATCGCCGA CGGCACGATC TCGAACAAGA TCGCGAAGGA AATCTTCGTG GCGATCTGGG ACGAGAAGGC GGCCGACGAA GGCGCGGCCG ACCGCATCAT CGATGCGAAG GGCCTGAAGC AGATCTCCGA TACGGGCGCG CTCGAGGCGA TCATCGACGA GGTGCTCGCC GCGAACGCGA AGTCGGTCGA GGAGTTCCGC GCGGGCAAGG AAAAGGCGTT CAACGCGCTC GTCGGCCAGG CGATGAAGGC GACGAAGGGC AAGGCGAACC CGCAGCAGGT CAACGAGCTC CTGAAGAAGA AGCTCGGCTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A