ButhA.00951.a

Glutamyl-tRNA(Gln) amidotransferase subunit A (Glu-ADT subunit A) (EC 6.3.5.-) (BTH_I0148) (gatA)

CENTER ID: ButhA.00951.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00951.a.A1.GE32751 full length 1 496
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.2792
RefSeq: YP_440706.1
UniProt: Q2T293

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHAKSLTELR AALDAKECSA VELAQHYLKR IDAARDLNAF VHVDAELTLA QAKAADAALA KGEAGPLAGL PIAHKDVFVT RGWRSTAGSK MLANYASPFD ATVVARLSAA GMVTLGKTNM DEFAMGSSNE NSAFGPVKNP WDTNAVPGGS SGGSSAAVAA RLAPAATGTD TGGSIRQPAS FAGVTGIKPT YGRVSRYGMI AFASSLDQGG PMARSAADCA LLLNAMAGFD ERDSTSLERA DEDYTRHLGK TWAAGGDAGK PLAGLRIGLP AEYFGAGLAD DVRAAIDAAL KTYETLGATL VPVSLPKTEL SIPVYYVIAP AEASSNLSRF DGVRYGHRAA EYRDLLDMYK KSRAEGFGPE VKRRILVGTY VLSHGYYDAY YLQAQKIRRI IAQDFQEAFK SCDVIMGPAS PTVAWDIGAK GDDPVQMYLA DIYTLSVSLA GLPGMSVPCG FGAGANAKRP VGLQIIGNYF DEARMLQVAD AFQRATDWHV QEPAGV
NT Sequence
atgcacgcaa aaagcctgac cgaactgcgc gccgcgctcg acgccaagga atgctcggcc gtcgagctgg cgcagcacta tctgaaacgg atcgacgccg cgcgcgatct gaacgcgttc gtccacgtcg acgccgagct gacgctcgcg caggcgaagg ccgccgacgc cgcgctcgcc aagggcgagg cgggcccgct cgccggcctg ccgatcgcgc acaaggacgt gttcgtcacg cgcggctggc gctccaccgc cggctcgaag atgctcgcga actacgcgag cccgttcgac gcgaccgtcg tcgcccgcct gtcggcggcc ggcatggtca cgctcggcaa gaccaacatg gacgagttcg cgatgggctc gtcgaacgag aactccgcgt tcggcccggt gaagaacccg tgggacacga acgcggtgcc gggcggcagc tcgggcggca gctccgcggc cgtcgccgcg cgcctcgcgc ccgccgcgac gggcaccgac acgggcggct cgatccgcca gccggcgtcg ttcgccggcg tgacgggcat caagccgacc tacgggcgcg tgtcgcgcta cgggatgatc gcgttcgcgt cgtcgctcga ccagggcggc ccgatggcgc ggagcgcggc cgactgcgcg ctcctgctga acgcgatggc gggcttcgac gagcgcgatt cgacgagcct cgagcgcgcc gacgaagact acacgcgcca cctcggcaag acctgggcgg ccggcggcga cgcgggcaag ccgctcgccg gcctgcgcat cggcctgccg gccgagtatt tcggcgcggg cctcgccgac gacgtgcgcg cggcgatcga cgcggcgctc aagacctatg agacgctcgg cgcgacgctc gtgccggtgt cgctgccgaa gaccgagctg tcgatccccg tctactacgt gatcgcgccc gccgaggcgt cgtcgaacct gtcgcgcttc gacggcgtgc gctacggtca ccgcgcggcc gaataccgcg atctgctcga catgtacaag aagtcgcgcg ccgagggctt cgggcccgaa gtgaagcgcc ggattctggt cggcacgtac gtgctgtcgc acggctacta cgacgcgtac tacctgcagg cgcagaagat ccgccgcatc atcgcgcagg atttccagga agcgttcaaa tcctgcgacg tgatcatggg cccggcatcg cccaccgtcg cgtgggacat cggcgcgaag ggcgacgatc ccgtccagat gtatctcgcg gacatctaca cgctgtcggt gagcctcgcg ggcctgcccg gcatgagcgt gccgtgcggc ttcggcgcgg gcgcgaacgc gaagcgcccg gtcggcctgc agatcatcgg caactatttc gacgaagccc ggatgctgca ggtcgccgac gcgttccagc gcgcgaccga ctggcacgta caagaaccgg caggagtgtg a
Details for ButhA.00951.a.A1.GE32751
HARVESTED ON: 6/20/2011
SEQUENCED ON: 7/14/2011
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 92
PERCENT COVERAGE: 51
Validated AA Sequence
MGTLEAQTQG PGSMHAKSLT ELRAALDAKE CSAVELAQHY LKRIDAARDL NAFVHVDAEL TLAQAKAADA ALAKGEAGPL AGLPIAHKDV FVTRGWRSTA GSKMLANYAS PFDATVVARL SAAGMVTLGK TNMDEFAMGS SNENSAFGPV KNPWDTNAVP GGSSGGSSAA VAARLAPAAT GTDTGGSIXX PASFAGVTGI KPTYGRVSRY GMIAFASSLD QXXPXARSAA DCALLLXAMA GFDXXDSTXL ERXDXTTRAX SAXPGRX
Validated NT Sequence
ccntggntca tcaccntcac catcatatgg gtaccctgga agctcagacc cagggtcctg gttcgatgca cgcaaaaagc ctgaccgaac tgcgcgccgc gctcgacgcc aaggaatgct cggccgtcga gctggcgcag cactatctga aacggatcga cgccgcgcgc gatctgaacg cgttcgtcca cgtcgacgcc gagctgacgc tcgcgcaggc gaaggccgcc gacgccgcgc tcgccaaggg cgaggcgggc ccgctcgccg gcctgccgat cgcgcacaag gacgtgttcg tcacgcgcgg ctggcgctcc accgccggct cgaagatgct cgcgaactac gcgagcccgt tcgacgcgac cgtcgtcgcc cgcctgtcgg cggccggcat ggtcacgctc ggcaagacca acatggacga gttcgcgatg ggctcgtcga acgagaactc cgcgttcggc ccggtgaaga acccgtggga cacgaacgcg gtgccgggcg gcagctcggg cggcagctcc gcggccgtcg ccgcgcgcct cgcgcccgcc gcgacnggca ccgacacggg cggctcgatc cnnnngccgg cgtcgttcgc cggcgtgacg ggcatcaagc cgacctacgg gcgcgtgtcg cgctacggga tgatcgcgtt cgcgtcgtcn ctcgaccagg nnnncccgat ngcgcggagc gcggccgact gcgcgctcct gctgancgcg atggcgggct tcgacgannn cgattcnacn agnctcgagc gnnccgacna nactacacgc gccncctcgg cangacctgg gcggncggng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMHAKSLTEL RAALDAKECS AVELAQHYLK RIDAARDLNA FVHVDAELTL AQAKAADAAL AKGEAGPLAG LPIAHKDVFV TRGWRSTAGS KMLANYASPF DATVVARLSA AGMVTLGKTN MDEFAMGSSN ENSAFGPVKN PWDTNAVPGG SSGGSSAAVA ARLAPAATGT DTGGSIRQPA SFAGVTGIKP TYGRVSRYGM IAFASSLDQG GPMARSAADC ALLLNAMAGF DERDSTSLER ADEDYTRHLG KTWAAGGDAG KPLAGLRIGL PAEYFGAGLA DDVRAAIDAA LKTYETLGAT LVPVSLPKTE LSIPVYYVIA PAEASSNLSR FDGVRYGHRA AEYRDLLDMY KKSRAEGFGP EVKRRILVGT YVLSHGYYDA YYLQAQKIRR IIAQDFQEAF KSCDVIMGPA SPTVAWDIGA KGDDPVQMYL ADIYTLSVSL AGLPGMSVPC GFGAGANAKR PVGLQIIGNY FDEARMLQVA DAFQRATDWH VQEPAGV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcacgcaaaa agcctgaccg aactgcgcgc cgcgctcgac gccaaggaat gctcggccgt cgagctggcg cagcactatc tgaaacggat cgacgccgcg cgcgatctga acgcgttcgt ccacgtcgac gccgagctga cgctcgcgca ggcgaaggcc gccgacgccg cgctcgccaa gggcgaggcg ggcccgctcg ccggcctgcc gatcgcgcac aaggacgtgt tcgtcacgcg cggctggcgc tccaccgccg gctcgaagat gctcgcgaac tacgcgagcc cgttcgacgc gaccgtcgtc gcccgcctgt cggcggccgg catggtcacg ctcggcaaga ccaacatgga cgagttcgcg atgggctcgt cgaacgagaa ctccgcgttc ggcccggtga agaacccgtg ggacacgaac gcggtgccgg gcggcagctc gggcggcagc tccgcggccg tcgccgcgcg cctcgcgccc gccgcgacgg gcaccgacac gggcggctcg atccgccagc cggcgtcgtt cgccggcgtg acgggcatca agccgaccta cgggcgcgtg tcgcgctacg ggatgatcgc gttcgcgtcg tcgctcgacc agggcggccc gatggcgcgg agcgcggccg actgcgcgct cctgctgaac gcgatggcgg gcttcgacga gcgcgattcg acgagcctcg agcgcgccga cgaagactac acgcgccacc tcggcaagac ctgggcggcc ggcggcgacg cgggcaagcc gctcgccggc ctgcgcatcg gcctgccggc cgagtatttc ggcgcgggcc tcgccgacga cgtgcgcgcg gcgatcgacg cggcgctcaa gacctatgag acgctcggcg cgacgctcgt gccggtgtcg ctgccgaaga ccgagctgtc gatccccgtc tactacgtga tcgcgcccgc cgaggcgtcg tcgaacctgt cgcgcttcga cggcgtgcgc tacggtcacc gcgcggccga ataccgcgat ctgctcgaca tgtacaagaa gtcgcgcgcc gagggcttcg ggcccgaagt gaagcgccgg attctggtcg gcacgtacgt gctgtcgcac ggctactacg acgcgtacta cctgcaggcg cagaagatcc gccgcatcat cgcgcaggat ttccaggaag cgttcaaatc ctgcgacgtg atcatgggcc cggcatcgcc caccgtcgcg tgggacatcg gcgcgaaggg cgacgatccc gtccagatgt atctcgcgga catctacacg ctgtcggtga gcctcgcggg cctgcccggc atgagcgtgc cgtgcggctt cggcgcgggc gcgaacgcga agcgcccggt cggcctgcag atcatcggca actatttcga cgaagcccgg atgctgcagg tcgccgacgc gttccagcgc gcgaccgact ggcacgtaca agaaccggca ggagtgtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa