ButhA.00944.a
Ribose-5-phosphate isomerase A (EC 5.3.1.6) (Phosphoriboisomerase A) (BTH_I2516) (rpiA)
| CENTER ID: | ButhA.00944.a |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00944.a.A1.GE32807 | full length | 1 | 235 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00944.a.A1.PW33411 | full length | 1 | 235 |
Structures
3U7J
DEPOSITED: 10/13/2011
DETERMINATION: XRay
CLONE: ButhA.00944.a.A1.GE32807
PROTEIN: ButhA.00944.a.A1.PW33411
3UW1
DEPOSITED: 11/30/2011
DETERMINATION: XRay
CLONE: ButhA.00944.a.A1.GE32807
PROTEIN: ButhA.00944.a.A1.PW33411
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127414 |
| PATRIC ID: | fig|271848.6.peg.5369 |
| RefSeq: | YP_443035.1 |
| UniProt: | Q2SVL4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTLLMTQDEL KRLVGEAAAR YVTDNVPQGA VIGVGTGSTA NCFIDALAAV KDRYRGAVSS SVATTERLKS HGIRVFDLNE IESLQVYVDG ADEIDESGAM IKGGGGALTR EKIVASVAET FVCIADASKR VAMLGQFPLP VEVVPMARTA IGRRLAALGG VPVLRVKQDG TPYVTDNGNE ILDVKGLRID DPRALEAAIN GWPGVVTVGL FAQRGADLCL LGTEHGVETL RYAAR
NT Sequence
ttgacgcttc tcatgaccca agacgaactc aaacgcctcg tcggcgaggc cgccgcccga tacgtgaccg acaacgtgcc gcaaggcgcg gtgatcggcg tcggcacggg ctcgaccgcc aactgcttca tcgacgcgct cgcggccgtc aaggatcgct atcgcggcgc ggtgtcgagt tcggtggcga cgaccgagcg cctgaagtcg cacggcatcc gggtgttcga tctgaacgag atcgaatcgc tgcaggtgta tgtcgacggc gccgacgaga tcgacgaaag cggcgcgatg atcaagggcg gcggcggcgc gcttacgcgc gagaagatcg tcgcttcggt cgcggaaacg ttcgtctgca tcgccgatgc gagcaagcgc gtcgcgatgc tcggccagtt tccgctgccc gtcgaggtcg tgccgatggc gcgcaccgcg atcggccgcc ggcttgccgc gctgggcggc gtgcccgtgc tgcgcgtgaa gcaggacggc acgccgtacg tgaccgacaa cggcaacgag attctcgacg tgaagggctt gcggatcgac gatccgcgcg cgctcgaagc ggcgatcaac ggctggcccg gcgtcgtgac ggtcggcctg ttcgcgcaac gcggcgcgga tctctgcctg ctcggcaccg agcacggcgt cgagacgctc cgttacgcgg cacgttaa
Details for ButhA.00944.a.A1.GE32807
| HARVESTED ON: | 6/28/2011 |
| SEQUENCED ON: | 7/22/2011 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLTLLMTQD ELKRLVGEAA ARYVTDNVPQ GAVIGVGTGS TANCFIDALA AVKDRYRGAV SSSVATTERL KSHGIRVFDL NEIESLQVYV DGADEIDESG AMIKGGGGAL TREKIVASVA ETFVCIADAS KRVAMLGQFP LPVEVVPMAR TAIGRRLAAL GGVPVLRVKQ DGTPYVTDNG NEILDVKGLR IDDPRALEAA INGWPGVVTV GLFAQRGADL CLLGTEHGVE TLRYAAR
Validated NT Sequence
ttattaacgt gccgcgtaac ggagcgtctc gacgccgtgc tcggtgccga gcaggcagag atccgcgccg cgttgcgcga acaggccgac cgtcacgacg ccgggccagc cgttgatcgc cgcttcgagc gcgcgcggat cgtcgatccg caagcccttc acgtcgagaa tctcgttgcc gttgtcggtc acgtacggcg tgccgtcctg cttcacgcgc agcacgggca cgccgcccag cgcggcaagc cggcggccga tcgcggtgcg cgccatcggc acgacctcga cgggcagcgg aaactggccg agcatcgcga cgcgcttgct cgcatcggcg atgcagacga acgtttccgc gaccgaagcg acgatcttct cgcgcgtaag cgcgccgccg ccgcccttga tcatcgcgcc gctttcgtcg atctcgtcgg cgccgtcgac atacacctgc agcgattcga tctcgttcag atcgaacacc cggatgccgt gcgacttcag gcgctcggtc gtcgccaccg aactcgacac cgcgccgcga tagcgatcct tgacggccgc gagcgcgtcg atgaagcagt tggcggtcga gcccgtgccg acgccgatca ccgcgccttg cggcacgttg tcggtcacgt atcgggcggc ggcctcgccg acgaggcgtt tgagttcgtc ttgggtcatg agaagcgtca acatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLTLLMTQD ELKRLVGEAA ARYVTDNVPQ GAVIGVGTGS TANCFIDALA AVKDRYRGAV SSSVATTERL KSHGIRVFDL NEIESLQVYV DGADEIDESG AMIKGGGGAL TREKIVASVA ETFVCIADAS KRVAMLGQFP LPVEVVPMAR TAIGRRLAAL GGVPVLRVKQ DGTPYVTDNG NEILDVKGLR IDDPRALEAA INGWPGVVTV GLFAQRGADL CLLGTEHGVE TLRYAAR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgacgctt ctcatgaccc aagacgaact caaacgcctc gtcggcgagg ccgccgcccg atacgtgacc gacaacgtgc cgcaaggcgc ggtgatcggc gtcggcacgg gctcgaccgc caactgcttc atcgacgcgc tcgcggccgt caaggatcgc tatcgcggcg cggtgtcgag ttcggtggcg acgaccgagc gcctgaagtc gcacggcatc cgggtgttcg atctgaacga gatcgaatcg ctgcaggtgt atgtcgacgg cgccgacgag atcgacgaaa gcggcgcgat gatcaagggc ggcggcggcg cgcttacgcg cgagaagatc gtcgcttcgg tcgcggaaac gttcgtctgc atcgccgatg cgagcaagcg cgtcgcgatg ctcggccagt ttccgctgcc cgtcgaggtc gtgccgatgg cgcgcaccgc gatcggccgc cggcttgccg cgctgggcgg cgtgcccgtg ctgcgcgtga agcaggacgg cacgccgtac gtgaccgaca acggcaacga gattctcgac gtgaagggct tgcggatcga cgatccgcgc gcgctcgaag cggcgatcaa cggctggccc ggcgtcgtga cggtcggcct gttcgcgcaa cgcggcgcgg atctctgcct gctcggcacc gagcacggcg tcgagacgct ccgttacgcg gcacgttaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for ButhA.00944.a.A1.PW33411
| PURIFICATION DATe: | 9/2/2011 |
| CONCENTRATION: | 33.52mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLTLLMTQD ELKRLVGEAA ARYVTDNVPQ GAVIGVGTGS TANCFIDALA AVKDRYRGAV SSSVATTERL KSHGIRVFDL NEIESLQVYV DGADEIDESG AMIKGGGGAL TREKIVASVA ETFVCIADAS KRVAMLGQFP LPVEVVPMAR TAIGRRLAAL GGVPVLRVKQ DGTPYVTDNG NEILDVKGLR IDDPRALEAA INGWPGVVTV GLFAQRGADL CLLGTEHGVE TLRYAAR
Validated NT Sequence
ttattaacgt gccgcgtaac ggagcgtctc gacgccgtgc tcggtgccga gcaggcagag atccgcgccg cgttgcgcga acaggccgac cgtcacgacg ccgggccagc cgttgatcgc cgcttcgagc gcgcgcggat cgtcgatccg caagcccttc acgtcgagaa tctcgttgcc gttgtcggtc acgtacggcg tgccgtcctg cttcacgcgc agcacgggca cgccgcccag cgcggcaagc cggcggccga tcgcggtgcg cgccatcggc acgacctcga cgggcagcgg aaactggccg agcatcgcga cgcgcttgct cgcatcggcg atgcagacga acgtttccgc gaccgaagcg acgatcttct cgcgcgtaag cgcgccgccg ccgcccttga tcatcgcgcc gctttcgtcg atctcgtcgg cgccgtcgac atacacctgc agcgattcga tctcgttcag atcgaacacc cggatgccgt gcgacttcag gcgctcggtc gtcgccaccg aactcgacac cgcgccgcga tagcgatcct tgacggccgc gagcgcgtcg atgaagcagt tggcggtcga gcccgtgccg acgccgatca ccgcgccttg cggcacgttg tcggtcacgt atcgggcggc ggcctcgccg acgaggcgtt tgagttcgtc ttgggtcatg agaagcgtca acatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLTLLMTQD ELKRLVGEAA ARYVTDNVPQ GAVIGVGTGS TANCFIDALA AVKDRYRGAV SSSVATTERL KSHGIRVFDL NEIESLQVYV DGADEIDESG AMIKGGGGAL TREKIVASVA ETFVCIADAS KRVAMLGQFP LPVEVVPMAR TAIGRRLAAL GGVPVLRVKQ DGTPYVTDNG NEILDVKGLR IDDPRALEAA INGWPGVVTV GLFAQRGADL CLLGTEHGVE TLRYAAR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGACGCTT CTCATGACCC AAGACGAACT CAAACGCCTC GTCGGCGAGG CCGCCGCCCG ATACGTGACC GACAACGTGC CGCAAGGCGC GGTGATCGGC GTCGGCACGG GCTCGACCGC CAACTGCTTC ATCGACGCGC TCGCGGCCGT CAAGGATCGC TATCGCGGCG CGGTGTCGAG TTCGGTGGCG ACGACCGAGC GCCTGAAGTC GCACGGCATC CGGGTGTTCG ATCTGAACGA GATCGAATCG CTGCAGGTGT ATGTCGACGG CGCCGACGAG ATCGACGAAA GCGGCGCGAT GATCAAGGGC GGCGGCGGCG CGCTTACGCG CGAGAAGATC GTCGCTTCGG TCGCGGAAAC GTTCGTCTGC ATCGCCGATG CGAGCAAGCG CGTCGCGATG CTCGGCCAGT TTCCGCTGCC CGTCGAGGTC GTGCCGATGG CGCGCACCGC GATCGGCCGC CGGCTTGCCG CGCTGGGCGG CGTGCCCGTG CTGCGCGTGA AGCAGGACGG CACGCCGTAC GTGACCGACA ACGGCAACGA GATTCTCGAC GTGAAGGGCT TGCGGATCGA CGATCCGCGC GCGCTCGAAG CGGCGATCAA CGGCTGGCCC GGCGTCGTGA CGGTCGGCCT GTTCGCGCAA CGCGGCGCGG ATCTCTGCCT GCTCGGCACC GAGCACGGCG TCGAGACGCT CCGTTACGCG GCACGTTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA