ButhA.00940.a

Undecaprenyl diphosphate synthase (EC 2.5.1.31) (BTH_I2031) (uppS)

CENTER ID: ButhA.00940.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00940.a.A1.GE32750 full length 1 260
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.4840
RefSeq: YP_442555.1
UniProt: Q2SWZ4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTYTSSTVRV PDVAAVPRHI AIIMDGNGRW ATERRLPRVA GHTRGVDAVR SVVEGCARSG VEYLTLFAFS SENWRRPNDE VSFLMRLFIT ALEREVGKLH ANGIRLRVIG DLTKFEPRIQ ALIRRAETKT ARNTRLTLTI AANYGGRWDI LQATRKLVEE AAREGRAAEI DEDAFARHLA MAYAPEPDLF IRTGGEQRIS NFLLWQLAYT EFYFTDTFWP DFDAGALAEA LASYTHRERR FGRTSAQLEP QAQDADSLSC
NT Sequence
atgacctata ccagctctac cgttcgcgtg cctgacgtcg ccgccgtgcc gcgtcacatc gcgatcatca tggacggcaa cggccgttgg gcgaccgagc gccgcctgcc gcgcgtcgcg ggacacacgc gcggtgtcga cgcggtgcgt tcggtcgtcg aggggtgcgc gcgctcgggc gtcgaatatc tgacgctctt cgcgttcagc tccgagaact ggcgccggcc gaacgacgaa gtgtcgttcc tcatgcgcct gttcatcacc gcgctcgagc gcgaggtcgg caagctgcac gcgaacggca tccggctgcg cgtcatcggc gatctgacga agttcgagcc gcgcatccag gcgctgatcc gccgcgcgga gacgaagacc gcgcgcaaca cacgcttgac gctgacgatc gccgcgaact acggcggtcg ttgggacatc ctgcaggcga cgcgcaagct cgtcgaagag gcggcgcgcg aagggcgcgc ggccgaaatc gacgaggacg cgttcgcccg ccatctggcg atggcctatg cgcccgagcc ggatctgttc atccgcacgg gcggcgagca gcgcatcagc aatttcctgc tctggcagct cgcgtacacc gagttctact tcaccgacac attctggccg gatttcgacg ccggcgcgct cgccgaggcg ctcgcgtcgt acacgcaccg cgaacgccgc ttcggccgca cgagcgcgca gctcgaaccc caggcccagg acgccgactc cctttcatgc tga
Details for ButhA.00940.a.A1.GE32750
HARVESTED ON: 6/20/2011
SEQUENCED ON: 7/14/2011
EXPECTED MW: 31kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 91
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTYTSSTVR VPDVAAVPRH IAIIMDGNGR WATERRLPRV AGHTRGVDAV RSVVEGCARS GVEYLTLFAF SSENWRRPND EVSFLMRLFI TALEREVGKL HANGIRLRVI GDLTKFEPRI QALIRRAETK TARNTRLTLT IAANYGGRWD ILQATRKLVX EAAREGRAAE IDEDAFARHL AMAYAPEPDL FIRTGGEQRI SNFLLWQLAY TEFYFTDTFW PDFDAXALXR XARVVHAPRT PXSAXXARSS NPXXXDADS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacct ataccagctc taccgttcgc gtgcctgacg tcgccgccgt gccgcgtcac atcgcgatca tcatggacgg caacggccgt tgggcgaccg agcgccgcct gccgcgcgtc gcgggacaca cgcgcggtgt cgacgcggtg cgttcggtcg tcgaggggtg cgcgcgctcg ggcgtcgaat atctgacgct cttcgcgttc agctccgaga actggcgccg gccgaacgac gaagtgtcgt tcctcatgcg cctgttcatc accgcgctcg agcgcgaggt cggcaagctg cacgcgaacg gcatccggct gcgcgtcatc ggcgatctga cgaagttcga gccgcgcatc caggcgctga tccgccgcgc ggagacgaag accgcgcgca acacacgctt gacgctgacg atcgccgcga actacggcgg tcgttgggac atcctgcagg cgacgcgcaa gctcgtcnaa gaggcggcgc gcgaagggcg cgcggccgaa atcgacgagg acgcgttcgc ccgccatctg gcgatggcct atgcgcccga gccggatctg ttcatccgca cgggcggcga gcagcgcatc agcaatttcc tgctctggca gctcgcgtac accgagttct acttcaccga cacattctgg ccggatttcg acgccnnngc gctcgnccga ngcgctcgcg tcgtacacgc accgcgaacg ccnncttcgg ccgnnngagc gcgcagctcg aacccnnngn ccnnngacgc cgactccctt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTYTSSTVR VPDVAAVPRH IAIIMDGNGR WATERRLPRV AGHTRGVDAV RSVVEGCARS GVEYLTLFAF SSENWRRPND EVSFLMRLFI TALEREVGKL HANGIRLRVI GDLTKFEPRI QALIRRAETK TARNTRLTLT IAANYGGRWD ILQATRKLVE EAAREGRAAE IDEDAFARHL AMAYAPEPDL FIRTGGEQRI SNFLLWQLAY TEFYFTDTFW PDFDAGALAE ALASYTHRER RFGRTSAQLE PQAQDADSLS C
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacctatacc agctctaccg ttcgcgtgcc tgacgtcgcc gccgtgccgc gtcacatcgc gatcatcatg gacggcaacg gccgttgggc gaccgagcgc cgcctgccgc gcgtcgcggg acacacgcgc ggtgtcgacg cggtgcgttc ggtcgtcgag gggtgcgcgc gctcgggcgt cgaatatctg acgctcttcg cgttcagctc cgagaactgg cgccggccga acgacgaagt gtcgttcctc atgcgcctgt tcatcaccgc gctcgagcgc gaggtcggca agctgcacgc gaacggcatc cggctgcgcg tcatcggcga tctgacgaag ttcgagccgc gcatccaggc gctgatccgc cgcgcggaga cgaagaccgc gcgcaacaca cgcttgacgc tgacgatcgc cgcgaactac ggcggtcgtt gggacatcct gcaggcgacg cgcaagctcg tcgaagaggc ggcgcgcgaa gggcgcgcgg ccgaaatcga cgaggacgcg ttcgcccgcc atctggcgat ggcctatgcg cccgagccgg atctgttcat ccgcacgggc ggcgagcagc gcatcagcaa tttcctgctc tggcagctcg cgtacaccga gttctacttc accgacacat tctggccgga tttcgacgcc ggcgcgctcg ccgaggcgct cgcgtcgtac acgcaccgcg aacgccgctt cggccgcacg agcgcgcagc tcgaacccca ggcccaggac gccgactccc tttcatgcta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a