SSGCID: Target Page

ButhA.00934.a

Bifunctional protein FolD [Includes: Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)] (BTH_I1861) (folD)

CENTER ID:	ButhA.00934.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00934.a.A1.GE32805	full length		1	285

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00934.a.A1.PW37269	full length		1	285

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.4667
RefSeq:	YP_442391.1
UniProt:	Q2SXF8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTATLIDGNA LSKTLRAQAA KRAAALAARG HRPGLAVILV GDNPASEVYV RNKIKACEDN GFFSLKDRYP ATLSEPELLA RIGELNRDPK IHGILVQLPL PAHIDSHKVI EAIAPEKDVD GFHVANAGAL LTGKPLFRPC TPYGVMKMFE AYKIPLQGAN AVVIGRSNIV GKPMALLLLE AGATVTICHS KTRDLAAHTR EADIVVAAVG KRNVLTADMV KPGATVIDVG MNRNDEGKLC GDVDFAGVSQ VAGHITPVPG GVGPMTITML LVNTIEAAER AAAAA

NT Sequence

atgacagcca ctctgatcga cggcaacgcc ctttcgaaga ccctgcgcgc gcaagcggcc aagcgcgccg ccgcgctcgc cgcgcgcggt caccggccgg gcctcgcggt gatcctcgtc ggcgacaacc cggcgagcga agtctacgtg cgcaacaaga tcaaggcgtg cgaggacaac ggcttcttct cgctgaagga ccgctatccg gcgacgctct ccgagcccga gctgctcgcg cgcatcggcg aactgaaccg cgatccgaag atccacggga ttctcgtgca gttgccgctg cccgcgcaca tcgacagcca caaggtgatc gaggcgatcg cgccggagaa ggacgtcgac ggctttcacg tcgccaacgc gggcgcgctg ctgacgggca agccgctctt ccgcccgtgc acgccgtacg gcgtgatgaa gatgttcgaa gcgtacaaga tcccgctgca gggcgcgaac gcggtcgtga tcggccgctc gaacatcgtc ggcaagccga tggcgctgct gctgctcgaa gcgggcgcga ccgtcacgat ctgccatagc aagacgcgcg acctcgccgc gcatacgcgc gaggccgaca tcgtcgtcgc ggcggtcggc aagcgcaacg tgctgaccgc cgacatggtg aagccgggcg cgaccgtgat cgacgtcggg atgaaccgca acgacgaagg caagctgtgc ggcgatgtcg acttcgcggg cgtctcgcag gtcgcgggcc acatcacgcc cgtgccgggc ggcgtcggcc cgatgacgat cacgatgctg ctcgtcaata cgatcgaagc cgccgagcgc gccgcggccg cggcctga

Details for ButhA.00934.a.A1.GE32805

HARVESTED ON:	6/28/2011
SEQUENCED ON:	7/22/2011
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMTATLIDGN ALSKTLRAQA AKRAAALAAR GHRPGLAVIL VGDNPASEVY VRNKIKACED NGFFSLKDRY PATLSEPELL ARIGELNRDP KIHGILVQLP LPAHIDSHKV IEAIAPEKDV DGFHVANAGA LLTGKPLFRP CTPYGVMKMF EAYKIPLQGA NAVVIGRSNI VGKPMALLLL EAGATVTICH SKTRDLAAHT READIVVAAV GKRNVLTADM VKPGATVIDV GMNRNDEGKL CGDVDFAGVS QVAGHITPVP GGVGPMTITM LLVNTIEAAE RAAAAA

Validated NT Sequence

ttattaggcc gcggccgcgg cgcgctcggc ggcttcgatc gtattgacga gcagcatcgt gatcgtcatc gggccgacgc cgcccggcac gggcgtgatg tggcccgcga cctgcgagac gcccgcgaag tcgacatcgc cgcacagctt gccttcgtcg ttgcggttca tcccgacgtc gatcacggtc gcgcccggct tcaccatgtc ggcggtcagc acgttgcgct tgccgaccgc cgcgacgacg atgtcggcct cgcgcgtatg cgcggcgagg tcgcgcgtct tgctatggca gatcgtgacg gtcgcgcccg cttcgagcag cagcagcgcc atcggcttgc cgacgatgtt cgagcggccg atcacgaccg cgttcgcgcc ctgcagcggg atcttgtacg cttcgaacat cttcatcacg ccgtacggcg tgcacgggcg gaagagcggc ttgcccgtca gcagcgcgcc cgcgttggcg acgtgaaagc cgtcgacgtc cttctccggc gcgatcgcct cgatcacctt gtggctgtcg atgtgcgcgg gcagcggcaa ctgcacgaga atcccgtgga tcttcggatc gcggttcagt tcgccgatgc gcgcgagcag ctcgggctcg gagagcgtcg ccggatagcg gtccttcagc gagaagaagc cgttgtcctc gcacgccttg atcttgttgc gcacgtagac ttcgctcgcc gggttgtcgc cgacgaggat caccgcgagg cccggccggt gaccgcgcgc ggcgagcgcg gcggcgcgct tggccgcttg cgcgcgcagg gtcttcgaaa gggcgttgcc gtcgatcaga gtggctgtca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccatggtata tctccttctt aaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacagccact ctgatcgacg gcaacgccct ttcgaagacc ctgcgcgcgc aagcggccaa gcgcgccgcc gcgctcgccg cgcgcggtca ccggccgggc ctcgcggtga tcctcgtcgg cgacaacccg gcgagcgaag tctacgtgcg caacaagatc aaggcgtgcg aggacaacgg cttcttctcg ctgaaggacc gctatccggc gacgctctcc gagcccgagc tgctcgcgcg catcggcgaa ctgaaccgcg atccgaagat ccacgggatt ctcgtgcagt tgccgctgcc cgcgcacatc gacagccaca aggtgatcga ggcgatcgcg ccggagaagg acgtcgacgg ctttcacgtc gccaacgcgg gcgcgctgct gacgggcaag ccgctcttcc gcccgtgcac gccgtacggc gtgatgaaga tgttcgaagc gtacaagatc ccgctgcagg gcgcgaacgc ggtcgtgatc ggccgctcga acatcgtcgg caagccgatg gcgctgctgc tgctcgaagc gggcgcgacc gtcacgatct gccatagcaa gacgcgcgac ctcgccgcgc atacgcgcga ggccgacatc gtcgtcgcgg cggtcggcaa gcgcaacgtg ctgaccgccg acatggtgaa gccgggcgcg accgtgatcg acgtcgggat gaaccgcaac gacgaaggca agctgtgcgg cgatgtcgac ttcgcgggcg tctcgcaggt cgcgggccac atcacgcccg tgccgggcgg cgtcggcccg atgacgatca cgatgctgct cgtcaatacg atcgaagccg ccgagcgcgc cgcggccgcg gcctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for ButhA.00934.a.A1.PW37269

PURIFICATION DATe:	3/27/2013
CONCENTRATION:	40.8mg/ml
OBSERVED MW:	33kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	14
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACAGCCACT CTGATCGACG GCAACGCCCT TTCGAAGACC CTGCGCGCGC AAGCGGCCAA GCGCGCCGCC GCGCTCGCCG CGCGCGGTCA CCGGCCGGGC CTCGCGGTGA TCCTCGTCGG CGACAACCCG GCGAGCGAAG TCTACGTGCG CAACAAGATC AAGGCGTGCG AGGACAACGG CTTCTTCTCG CTGAAGGACC GCTATCCGGC GACGCTCTCC GAGCCCGAGC TGCTCGCGCG CATCGGCGAA CTGAACCGCG ATCCGAAGAT CCACGGGATT CTCGTGCAGT TGCCGCTGCC CGCGCACATC GACAGCCACA AGGTGATCGA GGCGATCGCG CCGGAGAAGG ACGTCGACGG CTTTCACGTC GCCAACGCGG GCGCGCTGCT GACGGGCAAG CCGCTCTTCC GCCCGTGCAC GCCGTACGGC GTGATGAAGA TGTTCGAAGC GTACAAGATC CCGCTGCAGG GCGCGAACGC GGTCGTGATC GGCCGCTCGA ACATCGTCGG CAAGCCGATG GCGCTGCTGC TGCTCGAAGC GGGCGCGACC GTCACGATCT GCCATAGCAA GACGCGCGAC CTCGCCGCGC ATACGCGCGA GGCCGACATC GTCGTCGCGG CGGTCGGCAA GCGCAACGTG CTGACCGCCG ACATGGTGAA GCCGGGCGCG ACCGTGATCG ACGTCGGGAT GAACCGCAAC GACGAAGGCA AGCTGTGCGG CGATGTCGAC TTCGCGGGCG TCTCGCAGGT CGCGGGCCAC ATCACGCCCG TGCCGGGCGG CGTCGGCCCG ATGACGATCA CGATGCTGCT CGTCAATACG ATCGAAGCCG CCGAGCGCGC CGCGGCCGCG GCCTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA