ButhA.00896.a
Citrate synthase (EC 2.3.3.1) (BTH_II0665) (gltA)
| CENTER ID: | ButhA.00896.a |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00896.a.A1.GE32749 | full length | 1 | 433 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00896.a.A1.PW33353 | full length | 1 | 433 |
Structures
4XGH
DEPOSITED: 12/30/2014
DETERMINATION: XRay
CLONE: ButhA.00896.a.A1.GE32749
PROTEIN: ButhA.00896.a.A1.PW33353
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_128590 |
| PATRIC ID: | fig|271848.6.peg.750 |
| RefSeq: | YP_438864.1 |
| UniProt: | Q2T7I5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTPSDVKATL SFSDNSPSVE LPIYKGTMGP DVIDIRKLYG QTGKFTYDPG FMSTASCNSA ITYIDGDKGE LLYRGYPIDN LAQNADFLES CYLLLKGELP NAAQKKEFVA TVTKHTMVHE QMQFFFRGFR RDAHPMAILV AAVGALSAFY HDSLDINDPR HREVSAIRMI AKLPTLVAMA YKYSIGQPFV YPRNDLSYSA NFMRMMFANP CEEYKVNDVL VRALDRILIL HADHEQNAST STVRLAGSSG ANPFACIAAG IACLWGPAHG GANEAALNML EQIGTPDNIP EFIKQVKDKN SGVKLMGFGH RVYKNYDPRA KLMRETCYEV LNELGLHDDP LFKLAMQLEK IALEDEYFVS RKLYPNVDFY SGIVQRALGI PTSMFTCIFA MARTVGWIAQ WNEMIADPEQ KIGRPRQLFI GETPRAAKPI DQR
NT Sequence
atgaccccgt ctgatgttaa agccacgcta tcgttcagcg acaattcgcc gagcgtcgaa ctgccgatct acaagggtac gatgggcccc gacgtgatcg atatccgcaa gctgtacggc cagaccggca agttcacgta tgacccgggc ttcatgtcga cggcgtcgtg caactcggcg atcacgtaca tcgacggcga caagggcgaa ctgctgtacc gcggctaccc gatcgacaat ctcgcgcaaa acgcggactt cctcgaatcc tgctatctgc tgctcaaggg cgagctgccg aacgccgcgc agaagaagga gttcgtcgcc accgtcacga agcacacgat ggtgcacgag cagatgcagt tcttctttcg cggtttccgc cgcgacgcgc acccgatggc gattctcgtc gccgcggtcg gcgcgctgtc cgcgttctac cacgactcgc tcgacatcaa cgatccgcgt caccgcgaag tgtcggcgat ccgcatgatc gcgaagctgc cgacgctcgt cgcgatggcg tacaagtaca gcatcggcca gccgttcgtc tatccgcgca acgatctgtc gtacagcgcg aacttcatgc gcatgatgtt cgcgaacccg tgcgaagagt acaaggtcaa cgacgtgctc gtccgcgcgc tcgaccgcat cctgatcctg cacgccgacc acgagcagaa cgcgtcgacg tcgacggtcc gtctcgcggg ctcgtcgggc gcgaatccgt tcgcgtgcat cgcggccggc atcgcgtgtc tgtggggccc ggcgcacggc ggcgcgaacg aagcggcgct gaacatgctc gagcagatcg gcacgccgga caacattccc gagttcatca agcaggtgaa ggacaagaac tcgggcgtga agctgatggg cttcggccac cgcgtgtaca agaactacga tccgcgcgcg aagctgatgc gcgaaacgtg ctacgaagtg ctcaacgagc tcggcctgca cgacgatccg ctgttcaagc tcgcgatgca gctcgagaag atcgcgctcg aagacgagta cttcgtgtcg cgcaagctgt acccgaacgt cgacttctac tcgggcatcg tccagcgcgc gctcggcatc ccgacgtcga tgttcacctg catcttcgcg atggcgcgca cggtcggctg gatcgcgcag tggaacgaga tgatcgccga tcccgagcag aagatcggcc gtccgcggca gctcttcatc ggcgaaacgc cgcgcgcggc gaagccgatc gatcagcgct ga
Details for ButhA.00896.a.A1.GE32749
| HARVESTED ON: | 6/20/2011 |
| SEQUENCED ON: | 7/14/2011 |
| EXPECTED MW: | 50kDa |
| OBSERVED MW: | 48kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 60 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MGTLEAQTQG PGSMTPSDVK ATLSFSDNSP SVELPIYKGT MGPDVIDIRK LYGQTGKFTY DPGFMSTASC NSAITYIDGD KGELLYRGYP IDNLAQNADF LESCYLLLKG ELPNAAQKKE FVATVTKHTM VHEQMQFFFR GFRRDAHPMA ILVAAVGALS AFYHDSLDIN DPRHREVSAI RMIAKLPTLV AMAYKYSIGQ PFVYPRNDLS YSANFHAHDV REPVRRVQGQ RRARPRARPH PDPARRPRAE RVDVDGPSRG LVGRESVRVH RGRHRVSVGP GARRRERSGA EHARADRHAG QHSRVHQAGE GQELGREADG LRPPRVQELR SAREADARNV LRSAQRARPA RRSAVQARDA AREDRARRRV LRVAQAVPER RLLLGHRPAR ARHPDVDVHL HLRDGAHGRL DRAVERDDRR SRAEDRPSAA ALHRRNAARG EADR
Validated NT Sequence
ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgaccccg tctgatgtta aagccacgct atcgttcagc gacaattcgc cgagcgtcga actgccgatc tacaagggta cgatgggccc cgacgtgatc gatatccgca agctgtacgg ccagaccggc aagttcacgt atgacccggg cttcatgtcg acggcgtcgt gcaactcggc gatcacgtac atcgacggcg acaagggcga actgctgtac cgcggctacc cgatcgacaa tctcgcgcaa aacgcggact tcctcgaatc ctgctatctg ctgctcaagg gcgagctgcc gaacgccgcg cagaagaagg agttcgtcgc caccgtcacg aagcacacga tggtgcacga gcagatgcag ttcttctttc gcggtttccg ccgcgacgcg cacccgatgg cgattctcgt cgccgcggtc ggcgcgctgt ccgcgttcta ccacgactcg ctcgacatca acgatccgcg tcaccgcgaa gtgtcggcga tccgcatgat cgcgaagctg ccgacgctcg tcgcgatggc gtacaagtac agcatcggcc agccgttcgt ctatccgcgc aacgatctgt cgtacagcgc gaactttcat gcgcatgatg ttcgcgaacc cgtgcgaaga gtacaaggtc aacgacgtgc tcgtccgcgc gctcgaccgc atcctgatcc tgcacgccga ccacgagcag aacgcgtcga cgtcgacggt ccgtctcgcg ggctcgtcgg gcgcgaatcc gttcgcgtgc atcgcggccg gcatcgcgtg tctgtggggc ccggcgcacg gcggcgcgaa cgaagcggcg ctgaacatgc tcgagcagat cggcacgccg gacaacattc ccgagttcat caagcaggtg aaggacaaga actcgggcgt gaagctgatg ggcttcggcc accgcgtgta caagaactac gatccgcgcg cgaagctgat gcgcgaaacg tgctacgaag tgctcaacga gctcggcctg cacgacgatc cgctgttcaa gctcgcgatg cagctcgaga agatcgcgct cgaagacgag tacttcgtgt cgcgcaagct gtacccgaac gtcgacttct actcgggcat cgtccagcgc gcgctcggca tcccgacgtc gatgttcacc tgcatcttcg cgatggcgcg cacggtcggc tggatcgcgc agtggaacga gatgatcgcc gatcccgagc agaagatcgg ccgtccgcgg cagctcttca tcggcgaaac gccgcgcgcg gcgaagccga tcgat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTPSDVKAT LSFSDNSPSV ELPIYKGTMG PDVIDIRKLY GQTGKFTYDP GFMSTASCNS AITYIDGDKG ELLYRGYPID NLAQNADFLE SCYLLLKGEL PNAAQKKEFV ATVTKHTMVH EQMQFFFRGF RRDAHPMAIL VAAVGALSAF YHDSLDINDP RHREVSAIRM IAKLPTLVAM AYKYSIGQPF VYPRNDLSYS ANFMRMMFAN PCEEYKVNDV LVRALDRILI LHADHEQNAS TSTVRLAGSS GANPFACIAA GIACLWGPAH GGANEAALNM LEQIGTPDNI PEFIKQVKDK NSGVKLMGFG HRVYKNYDPR AKLMRETCYE VLNELGLHDD PLFKLAMQLE KIALEDEYFV SRKLYPNVDF YSGIVQRALG IPTSMFTCIF AMARTVGWIA QWNEMIADPE QKIGRPRQLF IGETPRAAKP IDQR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccccgtct gatgttaaag ccacgctatc gttcagcgac aattcgccga gcgtcgaact gccgatctac aagggtacga tgggccccga cgtgatcgat atccgcaagc tgtacggcca gaccggcaag ttcacgtatg acccgggctt catgtcgacg gcgtcgtgca actcggcgat cacgtacatc gacggcgaca agggcgaact gctgtaccgc ggctacccga tcgacaatct cgcgcaaaac gcggacttcc tcgaatcctg ctatctgctg ctcaagggcg agctgccgaa cgccgcgcag aagaaggagt tcgtcgccac cgtcacgaag cacacgatgg tgcacgagca gatgcagttc ttctttcgcg gtttccgccg cgacgcgcac ccgatggcga ttctcgtcgc cgcggtcggc gcgctgtccg cgttctacca cgactcgctc gacatcaacg atccgcgtca ccgcgaagtg tcggcgatcc gcatgatcgc gaagctgccg acgctcgtcg cgatggcgta caagtacagc atcggccagc cgttcgtcta tccgcgcaac gatctgtcgt acagcgcgaa cttcatgcgc atgatgttcg cgaacccgtg cgaagagtac aaggtcaacg acgtgctcgt ccgcgcgctc gaccgcatcc tgatcctgca cgccgaccac gagcagaacg cgtcgacgtc gacggtccgt ctcgcgggct cgtcgggcgc gaatccgttc gcgtgcatcg cggccggcat cgcgtgtctg tggggcccgg cgcacggcgg cgcgaacgaa gcggcgctga acatgctcga gcagatcggc acgccggaca acattcccga gttcatcaag caggtgaagg acaagaactc gggcgtgaag ctgatgggct tcggccaccg cgtgtacaag aactacgatc cgcgcgcgaa gctgatgcgc gaaacgtgct acgaagtgct caacgagctc ggcctgcacg acgatccgct gttcaagctc gcgatgcagc tcgagaagat cgcgctcgaa gacgagtact tcgtgtcgcg caagctgtac ccgaacgtcg acttctactc gggcatcgtc cagcgcgcgc tcggcatccc gacgtcgatg ttcacctgca tcttcgcgat ggcgcgcacg gtcggctgga tcgcgcagtg gaacgagatg atcgccgatc ccgagcagaa gatcggccgt ccgcggcagc tcttcatcgg cgaaacgccg cgcgcggcga agccgatcga tcagcgctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for ButhA.00896.a.A1.PW33353
| PURIFICATION DATe: | 8/13/2011 |
| CONCENTRATION: | 27.8mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 12 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 60 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMTPSDVK ATLSFSDNSP SVELPIYKGT MGPDVIDIRK LYGQTGKFTY DPGFMSTASC NSAITYIDGD KGELLYRGYP IDNLAQNADF LESCYLLLKG ELPNAAQKKE FVATVTKHTM VHEQMQFFFR GFRRDAHPMA ILVAAVGALS AFYHDSLDIN DPRHREVSAI RMIAKLPTLV AMAYKYSIGQ PFVYPRNDLS YSANFHAHDV REPVRRVQGQ RRARPRARPH PDPARRPRAE RVDVDGPSRG LVGRESVRVH RGRHRVSVGP GARRRERSGA EHARADRHAG QHSRVHQAGE GQELGREADG LRPPRVQELR SAREADARNV LRSAQRARPA RRSAVQARDA AREDRARRRV LRVAQAVPER RLLLGHRPAR ARHPDVDVHL HLRDGAHGRL DRAVERDDRR SRAEDRPSAA ALHRRNAARG EADR
Validated NT Sequence
ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgaccccg tctgatgtta aagccacgct atcgttcagc gacaattcgc cgagcgtcga actgccgatc tacaagggta cgatgggccc cgacgtgatc gatatccgca agctgtacgg ccagaccggc aagttcacgt atgacccggg cttcatgtcg acggcgtcgt gcaactcggc gatcacgtac atcgacggcg acaagggcga actgctgtac cgcggctacc cgatcgacaa tctcgcgcaa aacgcggact tcctcgaatc ctgctatctg ctgctcaagg gcgagctgcc gaacgccgcg cagaagaagg agttcgtcgc caccgtcacg aagcacacga tggtgcacga gcagatgcag ttcttctttc gcggtttccg ccgcgacgcg cacccgatgg cgattctcgt cgccgcggtc ggcgcgctgt ccgcgttcta ccacgactcg ctcgacatca acgatccgcg tcaccgcgaa gtgtcggcga tccgcatgat cgcgaagctg ccgacgctcg tcgcgatggc gtacaagtac agcatcggcc agccgttcgt ctatccgcgc aacgatctgt cgtacagcgc gaactttcat gcgcatgatg ttcgcgaacc cgtgcgaaga gtacaaggtc aacgacgtgc tcgtccgcgc gctcgaccgc atcctgatcc tgcacgccga ccacgagcag aacgcgtcga cgtcgacggt ccgtctcgcg ggctcgtcgg gcgcgaatcc gttcgcgtgc atcgcggccg gcatcgcgtg tctgtggggc ccggcgcacg gcggcgcgaa cgaagcggcg ctgaacatgc tcgagcagat cggcacgccg gacaacattc ccgagttcat caagcaggtg aaggacaaga actcgggcgt gaagctgatg ggcttcggcc accgcgtgta caagaactac gatccgcgcg cgaagctgat gcgcgaaacg tgctacgaag tgctcaacga gctcggcctg cacgacgatc cgctgttcaa gctcgcgatg cagctcgaga agatcgcgct cgaagacgag tacttcgtgt cgcgcaagct gtacccgaac gtcgacttct actcgggcat cgtccagcgc gcgctcggca tcccgacgtc gatgttcacc tgcatcttcg cgatggcgcg cacggtcggc tggatcgcgc agtggaacga gatgatcgcc gatcccgagc agaagatcgg ccgtccgcgg cagctcttca tcggcgaaac gccgcgcgcg gcgaagccga tcgat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTPSDVKAT LSFSDNSPSV ELPIYKGTMG PDVIDIRKLY GQTGKFTYDP GFMSTASCNS AITYIDGDKG ELLYRGYPID NLAQNADFLE SCYLLLKGEL PNAAQKKEFV ATVTKHTMVH EQMQFFFRGF RRDAHPMAIL VAAVGALSAF YHDSLDINDP RHREVSAIRM IAKLPTLVAM AYKYSIGQPF VYPRNDLSYS ANFMRMMFAN PCEEYKVNDV LVRALDRILI LHADHEQNAS TSTVRLAGSS GANPFACIAA GIACLWGPAH GGANEAALNM LEQIGTPDNI PEFIKQVKDK NSGVKLMGFG HRVYKNYDPR AKLMRETCYE VLNELGLHDD PLFKLAMQLE KIALEDEYFV SRKLYPNVDF YSGIVQRALG IPTSMFTCIF AMARTVGWIA QWNEMIADPE QKIGRPRQLF IGETPRAAKP IDQR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCCCGTCT GATGTTAAAG CCACGCTATC GTTCAGCGAC AATTCGCCGA GCGTCGAACT GCCGATCTAC AAGGGTACGA TGGGCCCCGA CGTGATCGAT ATCCGCAAGC TGTACGGCCA GACCGGCAAG TTCACGTATG ACCCGGGCTT CATGTCGACG GCGTCGTGCA ACTCGGCGAT CACGTACATC GACGGCGACA AGGGCGAACT GCTGTACCGC GGCTACCCGA TCGACAATCT CGCGCAAAAC GCGGACTTCC TCGAATCCTG CTATCTGCTG CTCAAGGGCG AGCTGCCGAA CGCCGCGCAG AAGAAGGAGT TCGTCGCCAC CGTCACGAAG CACACGATGG TGCACGAGCA GATGCAGTTC TTCTTTCGCG GTTTCCGCCG CGACGCGCAC CCGATGGCGA TTCTCGTCGC CGCGGTCGGC GCGCTGTCCG CGTTCTACCA CGACTCGCTC GACATCAACG ATCCGCGTCA CCGCGAAGTG TCGGCGATCC GCATGATCGC GAAGCTGCCG ACGCTCGTCG CGATGGCGTA CAAGTACAGC ATCGGCCAGC CGTTCGTCTA TCCGCGCAAC GATCTGTCGT ACAGCGCGAA CTTCATGCGC ATGATGTTCG CGAACCCGTG CGAAGAGTAC AAGGTCAACG ACGTGCTCGT CCGCGCGCTC GACCGCATCC TGATCCTGCA CGCCGACCAC GAGCAGAACG CGTCGACGTC GACGGTCCGT CTCGCGGGCT CGTCGGGCGC GAATCCGTTC GCGTGCATCG CGGCCGGCAT CGCGTGTCTG TGGGGCCCGG CGCACGGCGG CGCGAACGAA GCGGCGCTGA ACATGCTCGA GCAGATCGGC ACGCCGGACA ACATTCCCGA GTTCATCAAG CAGGTGAAGG ACAAGAACTC GGGCGTGAAG CTGATGGGCT TCGGCCACCG CGTGTACAAG AACTACGATC CGCGCGCGAA GCTGATGCGC GAAACGTGCT ACGAAGTGCT CAACGAGCTC GGCCTGCACG ACGATCCGCT GTTCAAGCTC GCGATGCAGC TCGAGAAGAT CGCGCTCGAA GACGAGTACT TCGTGTCGCG CAAGCTGTAC CCGAACGTCG ACTTCTACTC GGGCATCGTC CAGCGCGCGC TCGGCATCCC GACGTCGATG TTCACCTGCA TCTTCGCGAT GGCGCGCACG GTCGGCTGGA TCGCGCAGTG GAACGAGATG ATCGCCGATC CCGAGCAGAA GATCGGCCGT CCGCGGCAGC TCTTCATCGG CGAAACGCCG CGCGCGGCGA AGCCGATCGA TCAGCGCTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA