ButhA.00888.a
Adenylosuccinate synthetase (AMPSase) (AdSS) (EC 6.3.4.4) (IMP--aspartate ligase) (BTH_I2245) (purA)
| CENTER ID: | ButhA.00888.a |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00888.a.A1.GE32804 | full length | 1 | 448 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00888.a.A1.PW33408 | full length | 1 | 448 |
Structures
3UE9
DEPOSITED: 10/28/2011
DETERMINATION: XRay
CLONE: ButhA.00888.a.A1.GE32804
PROTEIN: ButhA.00888.a.A1.PW33408
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127011 |
| PATRIC ID: | fig|271848.6.peg.5066 |
| RefSeq: | YP_442766.1 |
| UniProt: | Q2SWD3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSASAVNVTP GRNVVVVGTQ WGDEGKGKIV DWLTDHAQGV VRFQGGHNAG HTLIIGGKKT ILRLIPSGIM REGVACYIGN GVVLSPEALF KEIGELEEAG LSVRERLFIS EATTLILPYH IAIDQAREAR KGAGKIGTTG RGIGPAYEDK VGRRALRVQD LFDARTFADR LRENLDFHNF VLTQYLGGAA VDFQATLDTM LGYADRLRPM VADVSRRLYE ENHAGRNLLF EGAQGTLLDI DHGTYPFVTS SNCVAGAAAA GAGVGPQKLN YILGITKAYC TRVGSGPFPS ELYDADNPSR QDQIGITLAN VGKEFGSVTG RPRRTGWLDA AALRRSIQIN GVSGLCMTKL DVLDGLDEVK LCVGYKIDGE DADLLPRGAA EVARCEPVYE TFGGWKESTV GINSWDALPA NARAYLTRVQ EVAGVPIDMV STGPDRDETI LLRHPFKV
NT Sequence
atgtctgcca gcgcagtgaa cgtgactccc gggcgcaacg tcgtcgtcgt ggggactcaa tggggtgatg aaggcaaagg caagatcgtc gactggctga cggaccacgc tcagggcgtc gtgcgtttcc agggcggcca caacgccggc cacaccctca tcatcggcgg caagaagacc atcttgcgcc tcatcccgtc gggcatcatg cgcgaaggcg tcgcctgcta catcggcaac ggtgtcgtcc tgtcccccga ggcactgttc aaggaaatcg gcgagctgga agaggccggc ctttccgttc gtgagcgcct gttcatttcc gaagcgacga cgctgatcct tccgtatcac atcgcgatcg accaggcgcg cgaagcgcgc aagggcgcgg gcaagatcgg cacgacgggc cgcggcatcg gccccgcgta tgaggacaag gtcggccgtc gcgcgctgcg cgtgcaggac ctgttcgacg cgcgaacgtt cgccgatcgc ctgcgcgaga atctcgactt ccacaatttc gtgctgacgc agtatctcgg cggcgcggcc gtcgatttcc aggcgacgct cgacacgatg ctcggctacg cggatcgcct gaggccgatg gtcgcggacg tgtcgcgccg tctgtacgaa gaaaaccacg cgggccgcaa cctgctgttc gaaggcgcgc agggcacgct gctcgatatc gatcacggca cctatccgtt cgtcacgtcg agcaactgcg tcgcgggcgc ggcggcggcg ggcgcgggcg tcggcccgca gaagcttaac tacatcctcg gcatcacgaa ggcgtattgc acgcgcgtcg gctcggggcc gttcccgagc gagttgtacg acgcggacaa tccgagtcgc caggatcaga tcggcatcac gctcgcgaac gtcggcaagg aattcggctc cgtcacgggc cgtccgcgcc gcaccggctg gctcgacgcg gccgcgcttc gccgctcgat tcagatcaac ggcgtgtcgg gcctttgcat gacgaaactc gacgtgctcg acggcctcga cgaagtgaag ctgtgcgtcg gctacaagat cgacggcgaa gatgctgatc tgctgccgcg cggcgcggct gaagtcgcgc gctgcgagcc cgtctacgag acgttcgggg gctggaagga aagcacggtc ggcatcaatt cgtgggacgc gctgcccgcg aacgcgcgtg cgtatctgac gcgcgtgcag gaagtggccg gcgtgccgat cgacatggtg tccaccggcc cggatcgcga cgagacgatc ctgctgcgtc atccgttcaa ggtgtaa
Details for ButhA.00888.a.A1.GE32804
| HARVESTED ON: | 6/28/2011 |
| SEQUENCED ON: | 7/22/2011 |
| EXPECTED MW: | 50kDa |
| OBSERVED MW: | 50kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 21 |
| PERCENT COVERAGE: | 96 |
Validated AA Sequence
MTQQDRLVAI RAGGHHVDRH AGHFLHARQI RTRVRGQRVP RIDADRAFLP APERLVDGLA ARDFSRAARQ QISIFAVDLV ADAQLHFVEA VEHVEFRHAK ARHAVDLNRA AKRGRVEPAG AARTARDGAE FLADVRERDA DLILATRIVR VVQLARERPR ADARAIRLRD AEDVVKLLRA DARARRRRAR DAVARRDERI GAVIDIEQRA LRAFEQQVAA RVVFFVQTAR HVRDHRPQAI RVAEHRVERR LEIDGRAAEI LRQHEIVEVE ILAQAIGERS RVEQVLHAQR ATADLVLIRG ADAAARRADL ARALARFARL VDRDVIRKDQ RRRFGNEQAL TNGKAGLFQL ADFLEQCLGG QDDTVADVAG DAFAHDARRD EAQDGLLAAD DEGVAGVVAA LETHDALSVV RQPVDDLAFA FITPLSPHDD DVAPGSHVHC AGRHRTRTLG LSFQ
Validated NT Sequence
tgaacggatg acgcagcagg atcgtctcgt cgcgatccgg gccggtggac accatgtcga tcggcacgcc ggccacttcc tgcacgcgcg tcagatacgc acgcgcgttc gcgggcagcg cgtcccacga attgatgccg accgtgcttt ccttccagcc cccgaacgtc tcgtagacgg gctcgcagcg cgcgacttca gccgcgccgc gcggcagcag atcagcatct tcgccgtcga tcttgtagcc gacgcacagc ttcacttcgt cgaggccgtc gagcacgtcg agtttcgtca tgcaaaggcc cgacacgccg ttgatctgaa tcgagcggcg aagcgcggcc gcgtcgagcc agccggtgcg gcgcggacgg cccgtgacgg agccgaattc cttgccgacg ttcgcgagcg tgatgccgat ctgatcctgg cgactcggat tgtccgcgtc gtacaactcg ctcgggaacg gccccgagcc gacgcgcgtg caatacgcct tcgtgatgcc gaggatgtag ttaagcttct gcgggccgac gcccgcgccc gccgccgccg cgcccgcgac gcagttgctc gacgtgacga acggataggt gccgtgatcg atatcgagca gcgtgccctg cgcgccttcg aacagcaggt tgcggcccgc gtggttttct tcgtacagac ggcgcgacac gtccgcgacc atcggcctca ggcgatccgc gtagccgagc atcgtgtcga gcgtcgcctg gaaatcgacg gccgcgccgc cgagatactg cgtcagcacg aaattgtgga agtcgagatt ctcgcgcagg cgatcggcga acgttcgcgc gtcgaacagg tcctgcacgc gcagcgcgcg acggccgacc ttgtcctcat acgcggggcc gatgccgcgg cccgtcgtgc cgatcttgcc cgcgcccttg cgcgcttcgc gcgcctggtc gatcgcgatg tgatacggaa ggatcagcgt cgtcgcttcg gaaatgaaca ggcgctcacg aacggaaagg ccggcctctt ccagctcgcc gatttccttg aacagtgcct cgggggacag gacgacaccg ttgccgatgt agcaggcgac gccttcgcgc atgatgcccg acgggatgag gcgcaagatg gtcttcttgc cgccgatgat gagggtgtgg ccggcgttgt ggccgccctg gaaacgcacg acgccctgag cgtggtccgt cagccagtcg acgatcttgc ctttgccttc atcaccccat tgagtcccca cgacgacgac gttgcgcccg ggagtcacgt tcactgcgct ggcagacatc gaaccaggac cctgggtctg agcttccagg g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSASAVNVT PGRNVVVVGT QWGDEGKGKI VDWLTDHAQG VVRFQGGHNA GHTLIIGGKK TILRLIPSGI MREGVACYIG NGVVLSPEAL FKEIGELEEA GLSVRERLFI SEATTLILPY HIAIDQAREA RKGAGKIGTT GRGIGPAYED KVGRRALRVQ DLFDARTFAD RLRENLDFHN FVLTQYLGGA AVDFQATLDT MLGYADRLRP MVADVSRRLY EENHAGRNLL FEGAQGTLLD IDHGTYPFVT SSNCVAGAAA AGAGVGPQKL NYILGITKAY CTRVGSGPFP SELYDADNPS RQDQIGITLA NVGKEFGSVT GRPRRTGWLD AAALRRSIQI NGVSGLCMTK LDVLDGLDEV KLCVGYKIDG EDADLLPRGA AEVARCEPVY ETFGGWKEST VGINSWDALP ANARAYLTRV QEVAGVPIDM VSTGPDRDET ILLRHPFKV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctgccagc gcagtgaacg tgactcccgg gcgcaacgtc gtcgtcgtgg ggactcaatg gggtgatgaa ggcaaaggca agatcgtcga ctggctgacg gaccacgctc agggcgtcgt gcgtttccag ggcggccaca acgccggcca caccctcatc atcggcggca agaagaccat cttgcgcctc atcccgtcgg gcatcatgcg cgaaggcgtc gcctgctaca tcggcaacgg tgtcgtcctg tcccccgagg cactgttcaa ggaaatcggc gagctggaag aggccggcct ttccgttcgt gagcgcctgt tcatttccga agcgacgacg ctgatccttc cgtatcacat cgcgatcgac caggcgcgcg aagcgcgcaa gggcgcgggc aagatcggca cgacgggccg cggcatcggc cccgcgtatg aggacaaggt cggccgtcgc gcgctgcgcg tgcaggacct gttcgacgcg cgaacgttcg ccgatcgcct gcgcgagaat ctcgacttcc acaatttcgt gctgacgcag tatctcggcg gcgcggccgt cgatttccag gcgacgctcg acacgatgct cggctacgcg gatcgcctga ggccgatggt cgcggacgtg tcgcgccgtc tgtacgaaga aaaccacgcg ggccgcaacc tgctgttcga aggcgcgcag ggcacgctgc tcgatatcga tcacggcacc tatccgttcg tcacgtcgag caactgcgtc gcgggcgcgg cggcggcggg cgcgggcgtc ggcccgcaga agcttaacta catcctcggc atcacgaagg cgtattgcac gcgcgtcggc tcggggccgt tcccgagcga gttgtacgac gcggacaatc cgagtcgcca ggatcagatc ggcatcacgc tcgcgaacgt cggcaaggaa ttcggctccg tcacgggccg tccgcgccgc accggctggc tcgacgcggc cgcgcttcgc cgctcgattc agatcaacgg cgtgtcgggc ctttgcatga cgaaactcga cgtgctcgac ggcctcgacg aagtgaagct gtgcgtcggc tacaagatcg acggcgaaga tgctgatctg ctgccgcgcg gcgcggctga agtcgcgcgc tgcgagcccg tctacgagac gttcgggggc tggaaggaaa gcacggtcgg catcaattcg tgggacgcgc tgcccgcgaa cgcgcgtgcg tatctgacgc gcgtgcagga agtggccggc gtgccgatcg acatggtgtc caccggcccg gatcgcgacg agacgatcct gctgcgtcat ccgttcaagg tgtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for ButhA.00888.a.A1.PW33408
| PURIFICATION DATe: | 8/19/2011 |
| CONCENTRATION: | 32.28mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 15 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 21 |
| PERCENT COVERAGE: | 96 |
Protocol Notes
notes unavailable
Validated AA Sequence
MTQQDRLVAI RAGGHHVDRH AGHFLHARQI RTRVRGQRVP RIDADRAFLP APERLVDGLA ARDFSRAARQ QISIFAVDLV ADAQLHFVEA VEHVEFRHAK ARHAVDLNRA AKRGRVEPAG AARTARDGAE FLADVRERDA DLILATRIVR VVQLARERPR ADARAIRLRD AEDVVKLLRA DARARRRRAR DAVARRDERI GAVIDIEQRA LRAFEQQVAA RVVFFVQTAR HVRDHRPQAI RVAEHRVERR LEIDGRAAEI LRQHEIVEVE ILAQAIGERS RVEQVLHAQR ATADLVLIRG ADAAARRADL ARALARFARL VDRDVIRKDQ RRRFGNEQAL TNGKAGLFQL ADFLEQCLGG QDDTVADVAG DAFAHDARRD EAQDGLLAAD DEGVAGVVAA LETHDALSVV RQPVDDLAFA FITPLSPHDD DVAPGSHVHC AGRHRTRTLG LSFQ
Validated NT Sequence
tgaacggatg acgcagcagg atcgtctcgt cgcgatccgg gccggtggac accatgtcga tcggcacgcc ggccacttcc tgcacgcgcg tcagatacgc acgcgcgttc gcgggcagcg cgtcccacga attgatgccg accgtgcttt ccttccagcc cccgaacgtc tcgtagacgg gctcgcagcg cgcgacttca gccgcgccgc gcggcagcag atcagcatct tcgccgtcga tcttgtagcc gacgcacagc ttcacttcgt cgaggccgtc gagcacgtcg agtttcgtca tgcaaaggcc cgacacgccg ttgatctgaa tcgagcggcg aagcgcggcc gcgtcgagcc agccggtgcg gcgcggacgg cccgtgacgg agccgaattc cttgccgacg ttcgcgagcg tgatgccgat ctgatcctgg cgactcggat tgtccgcgtc gtacaactcg ctcgggaacg gccccgagcc gacgcgcgtg caatacgcct tcgtgatgcc gaggatgtag ttaagcttct gcgggccgac gcccgcgccc gccgccgccg cgcccgcgac gcagttgctc gacgtgacga acggataggt gccgtgatcg atatcgagca gcgtgccctg cgcgccttcg aacagcaggt tgcggcccgc gtggttttct tcgtacagac ggcgcgacac gtccgcgacc atcggcctca ggcgatccgc gtagccgagc atcgtgtcga gcgtcgcctg gaaatcgacg gccgcgccgc cgagatactg cgtcagcacg aaattgtgga agtcgagatt ctcgcgcagg cgatcggcga acgttcgcgc gtcgaacagg tcctgcacgc gcagcgcgcg acggccgacc ttgtcctcat acgcggggcc gatgccgcgg cccgtcgtgc cgatcttgcc cgcgcccttg cgcgcttcgc gcgcctggtc gatcgcgatg tgatacggaa ggatcagcgt cgtcgcttcg gaaatgaaca ggcgctcacg aacggaaagg ccggcctctt ccagctcgcc gatttccttg aacagtgcct cgggggacag gacgacaccg ttgccgatgt agcaggcgac gccttcgcgc atgatgcccg acgggatgag gcgcaagatg gtcttcttgc cgccgatgat gagggtgtgg ccggcgttgt ggccgccctg gaaacgcacg acgccctgag cgtggtccgt cagccagtcg acgatcttgc ctttgccttc atcaccccat tgagtcccca cgacgacgac gttgcgcccg ggagtcacgt tcactgcgct ggcagacatc gaaccaggac cctgggtctg agcttccagg g
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSASAVNVT PGRNVVVVGT QWGDEGKGKI VDWLTDHAQG VVRFQGGHNA GHTLIIGGKK TILRLIPSGI MREGVACYIG NGVVLSPEAL FKEIGELEEA GLSVRERLFI SEATTLILPY HIAIDQAREA RKGAGKIGTT GRGIGPAYED KVGRRALRVQ DLFDARTFAD RLRENLDFHN FVLTQYLGGA AVDFQATLDT MLGYADRLRP MVADVSRRLY EENHAGRNLL FEGAQGTLLD IDHGTYPFVT SSNCVAGAAA AGAGVGPQKL NYILGITKAY CTRVGSGPFP SELYDADNPS RQDQIGITLA NVGKEFGSVT GRPRRTGWLD AAALRRSIQI NGVSGLCMTK LDVLDGLDEV KLCVGYKIDG EDADLLPRGA AEVARCEPVY ETFGGWKEST VGINSWDALP ANARAYLTRV QEVAGVPIDM VSTGPDRDET ILLRHPFKV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCTGCCAGC GCAGTGAACG TGACTCCCGG GCGCAACGTC GTCGTCGTGG GGACTCAATG GGGTGATGAA GGCAAAGGCA AGATCGTCGA CTGGCTGACG GACCACGCTC AGGGCGTCGT GCGTTTCCAG GGCGGCCACA ACGCCGGCCA CACCCTCATC ATCGGCGGCA AGAAGACCAT CTTGCGCCTC ATCCCGTCGG GCATCATGCG CGAAGGCGTC GCCTGCTACA TCGGCAACGG TGTCGTCCTG TCCCCCGAGG CACTGTTCAA GGAAATCGGC GAGCTGGAAG AGGCCGGCCT TTCCGTTCGT GAGCGCCTGT TCATTTCCGA AGCGACGACG CTGATCCTTC CGTATCACAT CGCGATCGAC CAGGCGCGCG AAGCGCGCAA GGGCGCGGGC AAGATCGGCA CGACGGGCCG CGGCATCGGC CCCGCGTATG AGGACAAGGT CGGCCGTCGC GCGCTGCGCG TGCAGGACCT GTTCGACGCG CGAACGTTCG CCGATCGCCT GCGCGAGAAT CTCGACTTCC ACAATTTCGT GCTGACGCAG TATCTCGGCG GCGCGGCCGT CGATTTCCAG GCGACGCTCG ACACGATGCT CGGCTACGCG GATCGCCTGA GGCCGATGGT CGCGGACGTG TCGCGCCGTC TGTACGAAGA AAACCACGCG GGCCGCAACC TGCTGTTCGA AGGCGCGCAG GGCACGCTGC TCGATATCGA TCACGGCACC TATCCGTTCG TCACGTCGAG CAACTGCGTC GCGGGCGCGG CGGCGGCGGG CGCGGGCGTC GGCCCGCAGA AGCTTAACTA CATCCTCGGC ATCACGAAGG CGTATTGCAC GCGCGTCGGC TCGGGGCCGT TCCCGAGCGA GTTGTACGAC GCGGACAATC CGAGTCGCCA GGATCAGATC GGCATCACGC TCGCGAACGT CGGCAAGGAA TTCGGCTCCG TCACGGGCCG TCCGCGCCGC ACCGGCTGGC TCGACGCGGC CGCGCTTCGC CGCTCGATTC AGATCAACGG CGTGTCGGGC CTTTGCATGA CGAAACTCGA CGTGCTCGAC GGCCTCGACG AAGTGAAGCT GTGCGTCGGC TACAAGATCG ACGGCGAAGA TGCTGATCTG CTGCCGCGCG GCGCGGCTGA AGTCGCGCGC TGCGAGCCCG TCTACGAGAC GTTCGGGGGC TGGAAGGAAA GCACGGTCGG CATCAATTCG TGGGACGCGC TGCCCGCGAA CGCGCGTGCG TATCTGACGC GCGTGCAGGA AGTGGCCGGC GTGCCGATCG ACATGGTGTC CACCGGCCCG GATCGCGACG AGACGATCCT GCTGCGTCAT CCGTTCAAGG TGTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA