ButhA.00863.a

Histidine protein kinase PhoR

CENTER ID: ButhA.00863.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00863.a.A2.GE34803 Mature protein( ButhA.00863.a ) 26 436
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.5630
RefSeq: YP_443279.1
UniProt: Q2SUX0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNIIWARFLV SLVLLALVSG IIGAIAGPVP GLVFALAMLV VQGFVSTFHT QRLWRLLDAP VYGEVPSAPG IWGEIYYRLH KLAKQWHAQV RQVEQQHSRF IQAIQASPNG VAMLDDRDQI EWCNAIAEVH FGLDAKRDLR QHITHLVRHP DFVRYLNGQH YDETLVMRGM GGNRQNVLAV QVFPYGENRK LLLTQDITEL ERTDAMRRDF VANVSHELKT PLTVLSGFLE TMRELPLSDD ERARYLELME QQASRMRHIV TDLLVLAKLE GESKPPADRA VDMRTVLDHL KDDAQTLSNG HHEITFKVDE TLAVTGAQTE IFSALGNLVT NAIRYTPDGG TIRVEWRRDG AQAVFSVTDS GLGIPAADLP RLTERFYRVD RSRSRDTGGT GLGLAIVKHV LQRHDAQLSI QSEEGRGSTF SARFPAHRTI SRRHAA
NT Sequence
ATGAACATCA TCTGGGCGCG CTTTCTGGTG TCGCTCGTCC TGCTCGCGCT CGTGAGCGGA ATCATCGGCG CAATCGCGGG CCCAGTGCCG GGCCTCGTCT TCGCGCTCGC GATGCTCGTC GTGCAGGGTT TCGTCAGCAC CTTCCACACG CAGCGCCTCT GGCGCCTGCT CGATGCGCCC GTCTACGGCG AAGTGCCGAG CGCGCCGGGC ATCTGGGGCG AGATCTACTA CCGGCTGCAC AAGCTCGCGA AGCAGTGGCA CGCGCAGGTT CGGCAGGTCG AGCAGCAGCA TTCGCGTTTC ATTCAGGCGA TCCAGGCGTC GCCGAACGGC GTCGCGATGC TCGACGACCG CGATCAGATC GAGTGGTGCA ACGCGATCGC GGAAGTGCAC TTCGGGCTCG ACGCGAAGCG CGACCTGCGC CAGCACATCA CGCATCTCGT GCGCCATCCG GATTTCGTCC GCTACCTGAA CGGCCAGCAT TACGACGAGA CGCTCGTGAT GCGCGGCATG GGCGGCAACC GGCAGAACGT GCTCGCCGTG CAGGTGTTTC CGTACGGGGA GAATCGCAAG CTGCTGCTCA CGCAGGACAT CACCGAGCTC GAGCGCACCG ATGCGATGCG GCGCGACTTC GTCGCGAACG TCTCGCATGA GCTGAAGACG CCGCTCACCG TGCTGTCGGG CTTTCTCGAG ACGATGCGCG AGCTGCCGCT GTCGGACGAC GAGCGCGCTC GCTATCTCGA GCTGATGGAG CAGCAGGCGT CGCGGATGCG GCACATCGTC ACCGATCTGC TCGTGCTCGC GAAGCTCGAA GGCGAGAGCA AGCCGCCCGC CGATCGGGCG GTCGACATGC GCACGGTGCT CGATCATCTG AAGGACGATG CGCAGACGCT GTCGAACGGC CATCACGAGA TCACGTTCAA GGTTGACGAG ACGCTCGCGG TGACGGGCGC GCAGACCGAG ATTTTCAGCG CGCTCGGCAA CCTCGTGACG AATGCGATCC GCTATACGCC TGACGGCGGG ACGATTCGCG TCGAATGGCG GCGCGACGGC GCGCAGGCTG TGTTTTCGGT CACCGACAGC GGCCTCGGCA TCCCGGCCGC CGATCTGCCG CGGCTCACCG AGCGTTTCTA TCGCGTCGAT CGCAGCCGCT CGCGCGACAC GGGCGGCACG GGGCTCGGGC TCGCGATCGT CAAGCACGTG CTGCAACGGC ACGACGCGCA ACTGTCGATC CAGAGCGAGG AAGGGCGAGG CAGCACGTTC AGCGCGCGCT TTCCCGCGCA TCGGACGATC AGCCGGCGAC ACGCGGCG
Details for ButhA.00863.a.A2.GE34803
HARVESTED ON: 3/13/2012
SEQUENCED ON: 3/12/2012
EXPECTED MW: 48kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MAGPVPGLVF ALAMLVVQGF VSTFHTQRLW RLLDAPVYGE VPSAPGIWGE IYYRLHKLAK QWHAQVRQVE QQHSRFIQAI QASPNGVAML DDRDQIEWCN AIAEVHFGLD AKRDLRQHIT HLVRHPDFVR YLNGQHYDET LVMRGMGGNR QNVLAVQVFP YGENRKLLLT QDITELERTD AMRRDFVANV SHELKTPLTV LSGFLETMRE LPLSDDERAR YLELMEQQAS RMRHIVTDLL VLAKLEGESK PPADRAVDMR TVLDHLKDDA QTLSNGHHEI TFKVDETLAV TGAQTEIFSA LGNLVTNAIR YTPDGGTIRV EWRRDGAQAV FSVTDSGLGI PAADLPRLTE RFYRVDRSRS RDTGGTGLGL AIVKHVLQRH DAQLSIQSEE GRGSTFSARF PAHRTISRRH AA
Validated NT Sequence
acgccgcgtg tcgccggctg atcgtccgat gcgcgggaaa gcgcgcgctg aacgtgctgc ctcgcccttc ctcgctctgg atcgacagtt gcgcgtcgtg ccgttgcagc acgtgcttga cgatcgcgag cccgagcccc gtgccgcccg tgtcgcgcga gcggctgcga tcgacgcgat agaaacgctc ggtgagccgc ggcagatcgg cggccgggat gccgaggccg ctgtcggtga ccgaaaacac agcctgcgcg ccgtcgcgcc gccattcgac gcgaatcgtc ccgccgtcag gcgtatagcg gatcgcattc gtcacgaggt tgccgagcgc gctgaaaatc tcggtctgcg cgcccgtcac cgcgagcgtc tcgtcaacct tgaacgtgat ctcgtgatgg ccgttcgaca gcgtctgcgc atcgtccttc agatgatcga gcaccgtgcg catgtcgacc gcccgatcgg cgggcggctt gctctcgcct tcgagcttcg cgagcacgag cagatcggtg acgatgtgcc gcatccgcga cgcctgctgc tccatcagct cgagatagcg agcgcgctcg tcgtccgaca gcggcagctc gcgcatcgtc tcgagaaagc ccgacagcac ggtgagcggc gtcttcagct catgcgagac gttcgcgacg aagtcgcgcc gcatcgcatc ggtgcgctcg agctcggtga tgtcctgcgt gagcagcagc ttgcgattct ccccgtacgg aaacacctgc acggcgagca cgttctgccg gttgccgccc atgccgcgca tcacgagcgt ctcgtcgtaa tgctggccgt tcaggtagcg gacgaaatcc ggatggcgca cgagatgcgt gatgtgctgg cgcaggtcgc gcttcgcgtc gagcccgaag tgcacttccg cgatcgcgtt gcaccactcg atctgatcgc ggtcgtcgag catcgcgacg ccgttcggcg acgcctggat cgcctgaatg aaacgcgaat gctgctgctc gacctgccga acctgcgcgt gccactgctt cgcgagcttg tgcagccggt agtagatctc gccccagatg cccggcgcgc tcggcacttc gccgtagacg ggcgcatcga gcaggcgcca gaggcgctgc gtgtggaagg tgctgacgaa accctgcacg acgagcatcg cgagcgcgaa gacgaggccc ggcactgggc ccgccatcga accaggaccc tgggtctgag cttccag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAGPVPGLV FALAMLVVQG FVSTFHTQRL WRLLDAPVYG EVPSAPGIWG EIYYRLHKLA KQWHAQVRQV EQQHSRFIQA IQASPNGVAM LDDRDQIEWC NAIAEVHFGL DAKRDLRQHI THLVRHPDFV RYLNGQHYDE TLVMRGMGGN RQNVLAVQVF PYGENRKLLL TQDITELERT DAMRRDFVAN VSHELKTPLT VLSGFLETMR ELPLSDDERA RYLELMEQQA SRMRHIVTDL LVLAKLEGES KPPADRAVDM RTVLDHLKDD AQTLSNGHHE ITFKVDETLA VTGAQTEIFS ALGNLVTNAI RYTPDGGTIR VEWRRDGAQA VFSVTDSGLG IPAADLPRLT ERFYRVDRSR SRDTGGTGLG LAIVKHVLQR HDAQLSIQSE EGRGSTFSAR FPAHRTISRR HAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgggccca gtgccgggcc tcgtcttcgc gctcgcgatg ctcgtcgtgc agggtttcgt cagcaccttc cacacgcagc gcctctggcg cctgctcgat gcgcccgtct acggcgaagt gccgagcgcg ccgggcatct ggggcgagat ctactaccgg ctgcacaagc tcgcgaagca gtggcacgcg caggttcggc aggtcgagca gcagcattcg cgtttcattc aggcgatcca ggcgtcgccg aacggcgtcg cgatgctcga cgaccgcgat cagatcgagt ggtgcaacgc gatcgcggaa gtgcacttcg ggctcgacgc gaagcgcgac ctgcgccagc acatcacgca tctcgtgcgc catccggatt tcgtccgcta cctgaacggc cagcattacg acgagacgct cgtgatgcgc ggcatgggcg gcaaccggca gaacgtgctc gccgtgcagg tgtttccgta cggggagaat cgcaagctgc tgctcacgca ggacatcacc gagctcgagc gcaccgatgc gatgcggcgc gacttcgtcg cgaacgtctc gcatgagctg aagacgccgc tcaccgtgct gtcgggcttt ctcgagacga tgcgcgagct gccgctgtcg gacgacgagc gcgctcgcta tctcgagctg atggagcagc aggcgtcgcg gatgcggcac atcgtcaccg atctgctcgt gctcgcgaag ctcgaaggcg agagcaagcc gcccgccgat cgggcggtcg acatgcgcac ggtgctcgat catctgaagg acgatgcgca gacgctgtcg aacggccatc acgagatcac gttcaaggtt gacgagacgc tcgcggtgac gggcgcgcag accgagattt tcagcgcgct cggcaacctc gtgacgaatg cgatccgcta tacgcctgac ggcgggacga ttcgcgtcga atggcggcgc gacggcgcgc aggctgtgtt ttcggtcacc gacagcggcc tcggcatccc ggccgccgat ctgccgcggc tcaccgagcg tttctatcgc gtcgatcgca gccgctcgcg cgacacgggc ggcacggggc tcgggctcgc gatcgtcaag cacgtgctgc aacggcacga cgcgcaactg tcgatccaga gcgaggaagg gcgaggcagc acgttcagcg cgcgctttcc cgcgcatcgg acgatcagcc ggcgacacgc ggcgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa