ButhA.00820.a
Dihydrodipicolinate reductase (DHPR) (EC 1.3.1.26) (BTH_I1208) (dapB)
CENTER ID:
ButhA.00820.a
ORGANISM:
Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
True
NIH RISK GROUP:
3
SELECT AGENT:
False
NIH PRIORITY
pathogens category:
IIIB
Ordering Clones & Proteins
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Clones*
CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO
AA
START
AA
STOP
ORDER
MATERIAL
ButhA.00820.a.A1.GE32801
full length
info
1
268
ButhA.00820.a.A1.GE32801
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
ButhA.00820.a.A1.PW33405
full length
info
1
268
PW33405
Structures
4F3Y
DEPOSITED: 5/9/2012
DETERMINATION: XRay
CLONE: ButhA.00820.a.A1.GE32801
PROTEIN: ButhA.00820.a.A1.PW33405
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PDB:
3D63 ,
3DAH ,
3EIZ ,
3EJ2 ,
3EK2 ,
3EZO ,
3F0F ,
3FTP ,
3GK0 ,
3GK3 ,
3GVF ,
3GWA ,
3GWE ,
3IML ,
3SZ8 ,
3T4C ,
3TML ,
3TMQ ,
3TXY ,
3U7J ,
3UE9 ,
3UK1 ,
3UK2 ,
3UND ,
3UPT ,
3URR ,
3UW1 ,
3UW2 ,
3UW3 ,
3V2I ,
3V7N ,
3V8H ,
3V9O ,
3V9P ,
3VAV ,
4DDO ,
4DFE ,
4DHE ,
4DHK ,
4DUT ,
4DZ4 ,
4E4T ,
4E51 ,
4EFI ,
4EG0 ,
4EGJ ,
4EK2 ,
4EQY ,
4EWG ,
4EX5 ,
4EXQ ,
4F2G ,
4F32 ,
4F3N ,
4F3Y ,
4F4H ,
4F7D ,
4FK8 ,
4FRY ,
4G1K ,
4G67 ,
4G6Z ,
4GHK ,
4H3Y ,
4H3Z ,
4H4G
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSMKIAIAG ASGRMGRMLI EAVLAAPDAT LVGALDRTGS PQLGQDAGAF LGKQTGVALT DDIERVCAEA DYLIDFTLPE GTLVHLDAAL RHDVKLVIGT TGFSEPQKAQ LRAAGEKIAL VFSANMSVGV NVTMKLLEFA AKQFAQGYDI EIIEAHHRHK VDAPSGTALM MGETIAAATG RSLDDCAVYG RHGVTGERDP STIGFSAIRG GDIVGDHTVL FAGIGERIEI THKSASRVSY AQGALRAARF LAGRDAGFFD MQDVLGLR
NT Sequence
atgtcgtcca tgaagattgc gattgctggt gcgtcaggcc ggatgggccg gatgctgatc gaagcggtgc tcgccgcgcc cgacgcgacg ctcgtcggcg cgctcgaccg cacgggctcg ccgcagctcg gccaggacgc gggtgcgttc ctcggcaagc agaccggggt cgcgctcacc gacgatatcg agcgcgtgtg cgccgaagcc gattacctga tcgacttcac gctccccgag ggcacgctcg tccacctgga cgcggcgctg cgccacgacg tgaagctcgt gatcggcacg acgggcttca gcgagccgca gaaggcgcag ttgcgcgcgg cgggcgagaa gatcgcgctc gtgttctcgg cgaacatgag cgtcggcgtg aacgtcacga tgaagctgct cgaattcgcg gcgaagcagt tcgcgcaggg ctacgacatc gagatcatcg aggcgcatca ccggcacaag gtcgacgcgc cgtccggcac cgcgctgatg atgggcgaga cgatcgccgc cgcgacgggc cgctcgctcg acgattgcgc ggtctacggc cgccacggcg tgacgggcga gcgcgatccg tcgacgatcg gtttttcggc gatccgcggc ggcgacatcg tcggcgatca cacggtgctc ttcgccggca tcggcgagcg catcgagatc acgcacaaat cggcgagccg cgtgtcatac gcgcaagggg cgctgcgcgc cgcgcgcttc ctcgccggcc gcgacgccgg tttcttcgac atgcaggacg tgctcggcct gcgctga
Details for ButhA.00820.a.A1.GE32801
HARVESTED ON:
6/28/2011
SEQUENCED ON:
7/22/2011
EXPECTED MW:
30kDa
OBSERVED MW:
31kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
Low Expression
SOLUBLE EXPRESSION LEVEL
Moderate Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100
Details for ButhA.00820.a.A1.PW33405
PURIFICATION DATe:
9/23/2011
CONCENTRATION:
28.98mg/ml
OBSERVED MW:
data unavailable
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
6
VIAL VOLUME:
200µl
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100