ButhA.00820.a
Dihydrodipicolinate reductase (DHPR) (EC 1.3.1.26) (BTH_I1208) (dapB)
| CENTER ID: | ButhA.00820.a |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00820.a.A1.GE32801 | full length | 1 | 268 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00820.a.A1.PW33405 | full length | 1 | 268 |
Structures
4F3Y
DEPOSITED: 5/9/2012
DETERMINATION: XRay
CLONE: ButhA.00820.a.A1.GE32801
PROTEIN: ButhA.00820.a.A1.PW33405
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_131385 |
| PATRIC ID: | fig|271848.6.peg.3964 |
| RefSeq: | YP_441755.1 |
| UniProt: | Q2SZ94 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSMKIAIAG ASGRMGRMLI EAVLAAPDAT LVGALDRTGS PQLGQDAGAF LGKQTGVALT DDIERVCAEA DYLIDFTLPE GTLVHLDAAL RHDVKLVIGT TGFSEPQKAQ LRAAGEKIAL VFSANMSVGV NVTMKLLEFA AKQFAQGYDI EIIEAHHRHK VDAPSGTALM MGETIAAATG RSLDDCAVYG RHGVTGERDP STIGFSAIRG GDIVGDHTVL FAGIGERIEI THKSASRVSY AQGALRAARF LAGRDAGFFD MQDVLGLR
NT Sequence
atgtcgtcca tgaagattgc gattgctggt gcgtcaggcc ggatgggccg gatgctgatc gaagcggtgc tcgccgcgcc cgacgcgacg ctcgtcggcg cgctcgaccg cacgggctcg ccgcagctcg gccaggacgc gggtgcgttc ctcggcaagc agaccggggt cgcgctcacc gacgatatcg agcgcgtgtg cgccgaagcc gattacctga tcgacttcac gctccccgag ggcacgctcg tccacctgga cgcggcgctg cgccacgacg tgaagctcgt gatcggcacg acgggcttca gcgagccgca gaaggcgcag ttgcgcgcgg cgggcgagaa gatcgcgctc gtgttctcgg cgaacatgag cgtcggcgtg aacgtcacga tgaagctgct cgaattcgcg gcgaagcagt tcgcgcaggg ctacgacatc gagatcatcg aggcgcatca ccggcacaag gtcgacgcgc cgtccggcac cgcgctgatg atgggcgaga cgatcgccgc cgcgacgggc cgctcgctcg acgattgcgc ggtctacggc cgccacggcg tgacgggcga gcgcgatccg tcgacgatcg gtttttcggc gatccgcggc ggcgacatcg tcggcgatca cacggtgctc ttcgccggca tcggcgagcg catcgagatc acgcacaaat cggcgagccg cgtgtcatac gcgcaagggg cgctgcgcgc cgcgcgcttc ctcgccggcc gcgacgccgg tttcttcgac atgcaggacg tgctcggcct gcgctga
Details for ButhA.00820.a.A1.GE32801
| HARVESTED ON: | 6/28/2011 |
| SEQUENCED ON: | 7/22/2011 |
| EXPECTED MW: | 30kDa |
| OBSERVED MW: | 31kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSSMKIAIA GASGRMGRML IEAVLAAPDA TLVGALDRTG SPQLGQDAGA FLGKQTGVAL TDDIERVCAE ADYLIDFTLP EGTLVHLDAA LRHDVKLVIG TTGFSEPQKA QLRAAGEKIA LVFSANMSVG VNVTMKLLEF AAKQFAQGYD IEIIEAHHRH KVDAPSGTAL MMGETIAAAT GRSLDDCAVY GRHGVTGERD PSTIGFSAIR GGDIVGDHTV LFAGIGERIE ITHKSASRVS YAQGALRAAR FLAGRDAGFF DMQDVLGLR
Validated NT Sequence
ttattagcgc aggccgagca cgtcctgcat gtcgaagaaa ccggcgtcgc ggccggcgag gaagcgcgcg gcgcgcagcg ccccttgcgc gtatgacacg cggctcgccg atttgtgcgt gatctcgatg cgctcgccga tgccggcgaa gagcaccgtg tgatcgccga cgatgtcgcc gccgcggatc gccgaaaaac cgatcgtcga cggatcgcgc tcgcccgtca cgccgtggcg gccgtagacc gcgcaatcgt cgagcgagcg gcccgtcgcg gcggcgatcg tctcgcccat catcagcgcg gtgccggacg gcgcgtcgac cttgtgccgg tgatgcgcct cgatgatctc gatgtcgtag ccctgcgcga actgcttcgc cgcgaattcg agcagcttca tcgtgacgtt cacgccgacg ctcatgttcg ccgagaacac gagcgcgatc ttctcgcccg ccgcgcgcaa ctgcgccttc tgcggctcgc tgaagcccgt cgtgccgatc acgagcttca cgtcgtggcg cagcgccgcg tccaggtgga cgagcgtgcc ctcggggagc gtgaagtcga tcaggtaatc ggcttcggcg cacacgcgct cgatatcgtc ggtgagcgcg accccggtct gcttgccgag gaacgcaccc gcgtcctggc cgagctgcgg cgagcccgtg cggtcgagcg cgccgacgag cgtcgcgtcg ggcgcggcga gcaccgcttc gatcagcatc cggcccatcc ggcctgacgc accagcaatc gcaatcttca tggacgacat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc catggtatat ctcc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSSMKIAIA GASGRMGRML IEAVLAAPDA TLVGALDRTG SPQLGQDAGA FLGKQTGVAL TDDIERVCAE ADYLIDFTLP EGTLVHLDAA LRHDVKLVIG TTGFSEPQKA QLRAAGEKIA LVFSANMSVG VNVTMKLLEF AAKQFAQGYD IEIIEAHHRH KVDAPSGTAL MMGETIAAAT GRSLDDCAVY GRHGVTGERD PSTIGFSAIR GGDIVGDHTV LFAGIGERIE ITHKSASRVS YAQGALRAAR FLAGRDAGFF DMQDVLGLR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgtccatg aagattgcga ttgctggtgc gtcaggccgg atgggccgga tgctgatcga agcggtgctc gccgcgcccg acgcgacgct cgtcggcgcg ctcgaccgca cgggctcgcc gcagctcggc caggacgcgg gtgcgttcct cggcaagcag accggggtcg cgctcaccga cgatatcgag cgcgtgtgcg ccgaagccga ttacctgatc gacttcacgc tccccgaggg cacgctcgtc cacctggacg cggcgctgcg ccacgacgtg aagctcgtga tcggcacgac gggcttcagc gagccgcaga aggcgcagtt gcgcgcggcg ggcgagaaga tcgcgctcgt gttctcggcg aacatgagcg tcggcgtgaa cgtcacgatg aagctgctcg aattcgcggc gaagcagttc gcgcagggct acgacatcga gatcatcgag gcgcatcacc ggcacaaggt cgacgcgccg tccggcaccg cgctgatgat gggcgagacg atcgccgccg cgacgggccg ctcgctcgac gattgcgcgg tctacggccg ccacggcgtg acgggcgagc gcgatccgtc gacgatcggt ttttcggcga tccgcggcgg cgacatcgtc ggcgatcaca cggtgctctt cgccggcatc ggcgagcgca tcgagatcac gcacaaatcg gcgagccgcg tgtcatacgc gcaaggggcg ctgcgcgccg cgcgcttcct cgccggccgc gacgccggtt tcttcgacat gcaggacgtg ctcggcctgc gctaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for ButhA.00820.a.A1.PW33405
| PURIFICATION DATe: | 9/23/2011 |
| CONCENTRATION: | 28.98mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSSMKIAIA GASGRMGRML IEAVLAAPDA TLVGALDRTG SPQLGQDAGA FLGKQTGVAL TDDIERVCAE ADYLIDFTLP EGTLVHLDAA LRHDVKLVIG TTGFSEPQKA QLRAAGEKIA LVFSANMSVG VNVTMKLLEF AAKQFAQGYD IEIIEAHHRH KVDAPSGTAL MMGETIAAAT GRSLDDCAVY GRHGVTGERD PSTIGFSAIR GGDIVGDHTV LFAGIGERIE ITHKSASRVS YAQGALRAAR FLAGRDAGFF DMQDVLGLR
Validated NT Sequence
ttattagcgc aggccgagca cgtcctgcat gtcgaagaaa ccggcgtcgc ggccggcgag gaagcgcgcg gcgcgcagcg ccccttgcgc gtatgacacg cggctcgccg atttgtgcgt gatctcgatg cgctcgccga tgccggcgaa gagcaccgtg tgatcgccga cgatgtcgcc gccgcggatc gccgaaaaac cgatcgtcga cggatcgcgc tcgcccgtca cgccgtggcg gccgtagacc gcgcaatcgt cgagcgagcg gcccgtcgcg gcggcgatcg tctcgcccat catcagcgcg gtgccggacg gcgcgtcgac cttgtgccgg tgatgcgcct cgatgatctc gatgtcgtag ccctgcgcga actgcttcgc cgcgaattcg agcagcttca tcgtgacgtt cacgccgacg ctcatgttcg ccgagaacac gagcgcgatc ttctcgcccg ccgcgcgcaa ctgcgccttc tgcggctcgc tgaagcccgt cgtgccgatc acgagcttca cgtcgtggcg cagcgccgcg tccaggtgga cgagcgtgcc ctcggggagc gtgaagtcga tcaggtaatc ggcttcggcg cacacgcgct cgatatcgtc ggtgagcgcg accccggtct gcttgccgag gaacgcaccc gcgtcctggc cgagctgcgg cgagcccgtg cggtcgagcg cgccgacgag cgtcgcgtcg ggcgcggcga gcaccgcttc gatcagcatc cggcccatcc ggcctgacgc accagcaatc gcaatcttca tggacgacat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc catggtatat ctcc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSSMKIAIA GASGRMGRML IEAVLAAPDA TLVGALDRTG SPQLGQDAGA FLGKQTGVAL TDDIERVCAE ADYLIDFTLP EGTLVHLDAA LRHDVKLVIG TTGFSEPQKA QLRAAGEKIA LVFSANMSVG VNVTMKLLEF AAKQFAQGYD IEIIEAHHRH KVDAPSGTAL MMGETIAAAT GRSLDDCAVY GRHGVTGERD PSTIGFSAIR GGDIVGDHTV LFAGIGERIE ITHKSASRVS YAQGALRAAR FLAGRDAGFF DMQDVLGLR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCGTCCATG AAGATTGCGA TTGCTGGTGC GTCAGGCCGG ATGGGCCGGA TGCTGATCGA AGCGGTGCTC GCCGCGCCCG ACGCGACGCT CGTCGGCGCG CTCGACCGCA CGGGCTCGCC GCAGCTCGGC CAGGACGCGG GTGCGTTCCT CGGCAAGCAG ACCGGGGTCG CGCTCACCGA CGATATCGAG CGCGTGTGCG CCGAAGCCGA TTACCTGATC GACTTCACGC TCCCCGAGGG CACGCTCGTC CACCTGGACG CGGCGCTGCG CCACGACGTG AAGCTCGTGA TCGGCACGAC GGGCTTCAGC GAGCCGCAGA AGGCGCAGTT GCGCGCGGCG GGCGAGAAGA TCGCGCTCGT GTTCTCGGCG AACATGAGCG TCGGCGTGAA CGTCACGATG AAGCTGCTCG AATTCGCGGC GAAGCAGTTC GCGCAGGGCT ACGACATCGA GATCATCGAG GCGCATCACC GGCACAAGGT CGACGCGCCG TCCGGCACCG CGCTGATGAT GGGCGAGACG ATCGCCGCCG CGACGGGCCG CTCGCTCGAC GATTGCGCGG TCTACGGCCG CCACGGCGTG ACGGGCGAGC GCGATCCGTC GACGATCGGT TTTTCGGCGA TCCGCGGCGG CGACATCGTC GGCGATCACA CGGTGCTCTT CGCCGGCATC GGCGAGCGCA TCGAGATCAC GCACAAATCG GCGAGCCGCG TGTCATACGC GCAAGGGGCG CTGCGCGCCG CGCGCTTCCT CGCCGGCCGC GACGCCGGTT TCTTCGACAT GCAGGACGTG CTCGGCCTGC GCTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA