ButhA.00809.a

Argininosuccinate synthase (EC 6.3.4.5) (Citrulline--aspartate ligase) (BTH_I0279) (argG)

CENTER ID: ButhA.00809.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00809.a.A1.GE32800 full length 1 446
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00809.a.A1.PS01209 full length 1 446

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.2937
RefSeq: YP_440837.1
UniProt: Q2T1W2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTILENLPA GQKVGIAFSG GLDTSAALHW MRIKGAVPYA YTANLGQPDE DDYDAIPKRA IQYGAEGARL IDCRAQLVAE GIAALQCGAF HISTAGVTYF NTTPIGRAVT GTMLVAAMKE DGVNIWGDGS TYKGNDIERF YRYGLLVNPD LKIYKPWLDQ QFIDELGGRA EMSEFMRQAG FEYKMSAEKA YSTDSNLLGA THEAKDLESL ESGIKIVNPI MGVAFWRDDV KIDKEAVTVR FEEGRPVALN GVEYKDAVEL LLEANRIGGR HGLGMSDQIE NRIIEAKSRG IYEAPGLALL YIAYERLVTG IHNEDTIEQY RENGRRLGRL LYQGRWFDPQ AIMLRETAQR WVARAVTGEV TVELRRGNDY SILATRSPNL TYQPERLSME KVQSTFSPRD RIGQLTMRNL DITDTRDKLR IYSEVGLLTP GESSALPKLK EDESGN
NT Sequence
atgaccacga ttcttgaaaa tctcccggcc ggccagaaag tcggcatcgc gttctccggc gggctcgaca cgagcgccgc gctgcactgg atgcgcatca agggggccgt tccttacgcg tacacggcca atctcggcca gcccgacgaa gacgactacg atgcgatccc gaagcgcgcg atccagtacg gcgccgaggg cgcacgcctg attgactgcc gcgcgcagct cgtggccgaa gggatcgcgg cgctccagtg cggcgcgttc cacatctcga cggccggcgt cacgtatttc aatacgaccc cgattggccg cgccgtgacg ggcacgatgc tcgtcgccgc gatgaaggag gacggcgtca acatctgggg cgacggcagc acgtacaagg gcaacgacat cgagcgcttc taccgctacg gcctgctcgt caatccggat ctgaagatct acaagccgtg gctcgatcag cagttcatcg acgagctggg cggccgcgcg gaaatgtccg aattcatgcg ccaggccggc ttcgagtaca agatgtcggc ggagaaggcg tattcgaccg attcgaacct gctgggcgcg acgcacgagg cgaaggatct cgagagcctc gagagcggca tcaagatcgt caatccgatc atgggcgtcg cgttctggcg cgacgacgtg aagatcgaca aggaagcggt gacggtccgc ttcgaagagg gccgccccgt cgcgctgaac ggcgtcgagt acaaggatgc ggtcgagctg ctgctcgagg cgaaccggat cggcggccgc cacggcctcg ggatgagcga ccagatcgag aaccggatca tcgaggcgaa gagccgcggc atctatgaag ccccggggct tgcactgctg tacatcgcgt acgagcgcct cgtcaccggc atccacaacg aagacacgat cgagcagtac cgcgagaacg gccgccgcct cggccgcctg ctgtaccagg gccgctggtt cgatccgcag gcgatcatgc tgcgcgagac cgcgcagcgc tgggtcgcgc gcgcggtcac gggcgaggtg acggtcgagc tgcgccgcgg caacgactat tcgattctcg ccacgcgctc gccgaacctg acgtaccagc cggagcgcct gtcgatggag aaggtgcagt cgacgttctc gccgcgcgac cggatcggcc agttgacgat gcgcaacctc gacatcaccg acacccgcga caagctgcgc atctactcgg aagtcggcct gctcacgccg ggcgaatcgt cggcgctgcc gaagctgaag gaagacgaga gcggcaactg a
Details for ButhA.00809.a.A1.GE32800
HARVESTED ON: 6/28/2011
SEQUENCED ON: 7/22/2011
EXPECTED MW: 52kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MTTILENLPA GQKVGIAFSG GLDTSAALHW MRIKGAVPYA YTANLGQPDE DDYDAIPKRA IQYGAEGARL IDCRAQLVAE GIAALQCGAF HISTAGVTYF NTTPIGRAVT GTMLVAAMKE DGVNIWGDGS TYKGNDIERF YRYGLLVNPD LKIYKPWLDQ QFIDELGGRA EMSEFMRQAG FEYKMSAEKA YSTDSNLLGA THEAKDLESL ESGIKIVNPI MGVAFWRDDV KIDKEAVTVR FEEGRPVALN GVEYKDAVEL LLEANRIGGR HGLGMSDQIE NRIIEAKSRG IYEAPGLALL YIAYERLVTG IHNEDTIEQY RENGRRLGRL LYQGRWFDPQ AIMLRETAQR WVARAVTGEV TVELRRGNDY SILATRSPNL TYQPERLSME KVQSTFSPRD RIGQLTMRNL DITDTRDKLR IYSEVGLLTP GESSALPKLK EDESG
Validated NT Sequence
tgccgctctc gtcttccttc agcttcggca gcgccgacga ttcgcccggc gtgagcaggc cgacttccga gtagatgcgc agcttgtcgc gggtgtcggt gatgtcgagg ttgcgcatcg tcaactggcc gatccggtcg cgcggcgaga acgtcgactg caccttctcc atcgacaggc gctccggctg gtacgtcagg ttcggcgagc gcgtggcgag aatcgaatag tcgttgccgc ggcgcagctc gaccgtcacc tcgcccgtga ccgcgcgcgc gacccagcgc tgcgcggtct cgcgcagcat gatcgcctgc ggatcgaacc agcggccctg gtacagcagg cggccgaggc ggcggccgtt ctcgcggtac tgctcgatcg tgtcttcgtt gtggatgccg gtgacgaggc gctcgtacgc gatgtacagc agtgcaagcc ccggggcttc atagatgccg cggctcttcg cctcgatgat ccggttctcg atctggtcgc tcatcccgag gccgtggcgg ccgccgatcc ggttcgcctc gagcagcagc tcgaccgcat ccttgtactc gacgccgttc agcgcgacgg ggcggccctc ttcgaagcgg accgtcaccg cttccttgtc gatcttcacg tcgtcgcgcc agaacgcgac gcccatgatc ggattgacga tcttgatgcc gctctcgagg ctctcgagat ccttcgcctc gtgcgtcgcg cccagcaggt tcgaatcggt cgaatacgcc ttctccgccg acatcttgta ctcgaagccg gcctggcgca tgaattcgga catttccgcg cggccgccca gctcgtcgat gaactgctga tcgagccacg gcttgtagat cttcagatcc ggattgacga gcaggccgta gcggtagaag cgctcgatgt cgttgccctt gtacgtgctg ccgtcgcccc agatgttgac gccgtcctcc ttcatcgcgg cgacgagcat cgtgcccgtc acggcgcggc caatcggggt cgtattgaaa tacgtgacgc cggccgtcga gatgtggaac gcgccgcact ggagcgccgc gatcccttcg gccacgagct gcgcgcggca gtcaatcagg cgtgcgccct cggcgccgta ctggatcgcg cgcttcggga tcgcatcgta gtcgtcttcg tcgggctggc cgagattggc cgtgtacgcg taaggaacgg cccccttgat gcgcatccag tgcagcgcgg cgctcgtgtc gagcccgccg gagaacgcga tgccgacttt ctggccggcc gggagatttt caagaatcgt ggtcatcgaa ccaggaccct gggtctgagc ttccaggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTILENLP AGQKVGIAFS GGLDTSAALH WMRIKGAVPY AYTANLGQPD EDDYDAIPKR AIQYGAEGAR LIDCRAQLVA EGIAALQCGA FHISTAGVTY FNTTPIGRAV TGTMLVAAMK EDGVNIWGDG STYKGNDIER FYRYGLLVNP DLKIYKPWLD QQFIDELGGR AEMSEFMRQA GFEYKMSAEK AYSTDSNLLG ATHEAKDLES LESGIKIVNP IMGVAFWRDD VKIDKEAVTV RFEEGRPVAL NGVEYKDAVE LLLEANRIGG RHGLGMSDQI ENRIIEAKSR GIYEAPGLAL LYIAYERLVT GIHNEDTIEQ YRENGRRLGR LLYQGRWFDP QAIMLRETAQ RWVARAVTGE VTVELRRGND YSILATRSPN LTYQPERLSM EKVQSTFSPR DRIGQLTMRN LDITDTRDKL RIYSEVGLLT PGESSALPKL KEDESGN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccacgatt cttgaaaatc tcccggccgg ccagaaagtc ggcatcgcgt tctccggcgg gctcgacacg agcgccgcgc tgcactggat gcgcatcaag ggggccgttc cttacgcgta cacggccaat ctcggccagc ccgacgaaga cgactacgat gcgatcccga agcgcgcgat ccagtacggc gccgagggcg cacgcctgat tgactgccgc gcgcagctcg tggccgaagg gatcgcggcg ctccagtgcg gcgcgttcca catctcgacg gccggcgtca cgtatttcaa tacgaccccg attggccgcg ccgtgacggg cacgatgctc gtcgccgcga tgaaggagga cggcgtcaac atctggggcg acggcagcac gtacaagggc aacgacatcg agcgcttcta ccgctacggc ctgctcgtca atccggatct gaagatctac aagccgtggc tcgatcagca gttcatcgac gagctgggcg gccgcgcgga aatgtccgaa ttcatgcgcc aggccggctt cgagtacaag atgtcggcgg agaaggcgta ttcgaccgat tcgaacctgc tgggcgcgac gcacgaggcg aaggatctcg agagcctcga gagcggcatc aagatcgtca atccgatcat gggcgtcgcg ttctggcgcg acgacgtgaa gatcgacaag gaagcggtga cggtccgctt cgaagagggc cgccccgtcg cgctgaacgg cgtcgagtac aaggatgcgg tcgagctgct gctcgaggcg aaccggatcg gcggccgcca cggcctcggg atgagcgacc agatcgagaa ccggatcatc gaggcgaaga gccgcggcat ctatgaagcc ccggggcttg cactgctgta catcgcgtac gagcgcctcg tcaccggcat ccacaacgaa gacacgatcg agcagtaccg cgagaacggc cgccgcctcg gccgcctgct gtaccagggc cgctggttcg atccgcaggc gatcatgctg cgcgagaccg cgcagcgctg ggtcgcgcgc gcggtcacgg gcgaggtgac ggtcgagctg cgccgcggca acgactattc gattctcgcc acgcgctcgc cgaacctgac gtaccagccg gagcgcctgt cgatggagaa ggtgcagtcg acgttctcgc cgcgcgaccg gatcggccag ttgacgatgc gcaacctcga catcaccgac acccgcgaca agctgcgcat ctactcggaa gtcggcctgc tcacgccggg cgaatcgtcg gcgctgccga agctgaagga agacgagagc ggcaactaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for ButhA.00809.a.A1.PS01209
PURIFICATION DATe: 9/16/2011
CONCENTRATION: 24.6mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MTTILENLPA GQKVGIAFSG GLDTSAALHW MRIKGAVPYA YTANLGQPDE DDYDAIPKRA IQYGAEGARL IDCRAQLVAE GIAALQCGAF HISTAGVTYF NTTPIGRAVT GTMLVAAMKE DGVNIWGDGS TYKGNDIERF YRYGLLVNPD LKIYKPWLDQ QFIDELGGRA EMSEFMRQAG FEYKMSAEKA YSTDSNLLGA THEAKDLESL ESGIKIVNPI MGVAFWRDDV KIDKEAVTVR FEEGRPVALN GVEYKDAVEL LLEANRIGGR HGLGMSDQIE NRIIEAKSRG IYEAPGLALL YIAYERLVTG IHNEDTIEQY RENGRRLGRL LYQGRWFDPQ AIMLRETAQR WVARAVTGEV TVELRRGNDY SILATRSPNL TYQPERLSME KVQSTFSPRD RIGQLTMRNL DITDTRDKLR IYSEVGLLTP GESSALPKLK EDESG
Validated NT Sequence
tgccgctctc gtcttccttc agcttcggca gcgccgacga ttcgcccggc gtgagcaggc cgacttccga gtagatgcgc agcttgtcgc gggtgtcggt gatgtcgagg ttgcgcatcg tcaactggcc gatccggtcg cgcggcgaga acgtcgactg caccttctcc atcgacaggc gctccggctg gtacgtcagg ttcggcgagc gcgtggcgag aatcgaatag tcgttgccgc ggcgcagctc gaccgtcacc tcgcccgtga ccgcgcgcgc gacccagcgc tgcgcggtct cgcgcagcat gatcgcctgc ggatcgaacc agcggccctg gtacagcagg cggccgaggc ggcggccgtt ctcgcggtac tgctcgatcg tgtcttcgtt gtggatgccg gtgacgaggc gctcgtacgc gatgtacagc agtgcaagcc ccggggcttc atagatgccg cggctcttcg cctcgatgat ccggttctcg atctggtcgc tcatcccgag gccgtggcgg ccgccgatcc ggttcgcctc gagcagcagc tcgaccgcat ccttgtactc gacgccgttc agcgcgacgg ggcggccctc ttcgaagcgg accgtcaccg cttccttgtc gatcttcacg tcgtcgcgcc agaacgcgac gcccatgatc ggattgacga tcttgatgcc gctctcgagg ctctcgagat ccttcgcctc gtgcgtcgcg cccagcaggt tcgaatcggt cgaatacgcc ttctccgccg acatcttgta ctcgaagccg gcctggcgca tgaattcgga catttccgcg cggccgccca gctcgtcgat gaactgctga tcgagccacg gcttgtagat cttcagatcc ggattgacga gcaggccgta gcggtagaag cgctcgatgt cgttgccctt gtacgtgctg ccgtcgcccc agatgttgac gccgtcctcc ttcatcgcgg cgacgagcat cgtgcccgtc acggcgcggc caatcggggt cgtattgaaa tacgtgacgc cggccgtcga gatgtggaac gcgccgcact ggagcgccgc gatcccttcg gccacgagct gcgcgcggca gtcaatcagg cgtgcgccct cggcgccgta ctggatcgcg cgcttcggga tcgcatcgta gtcgtcttcg tcgggctggc cgagattggc cgtgtacgcg taaggaacgg cccccttgat gcgcatccag tgcagcgcgg cgctcgtgtc gagcccgccg gagaacgcga tgccgacttt ctggccggcc gggagatttt caagaatcgt ggtcatcgaa ccaggaccct gggtctgagc ttccaggg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTILENLP AGQKVGIAFS GGLDTSAALH WMRIKGAVPY AYTANLGQPD EDDYDAIPKR AIQYGAEGAR LIDCRAQLVA EGIAALQCGA FHISTAGVTY FNTTPIGRAV TGTMLVAAMK EDGVNIWGDG STYKGNDIER FYRYGLLVNP DLKIYKPWLD QQFIDELGGR AEMSEFMRQA GFEYKMSAEK AYSTDSNLLG ATHEAKDLES LESGIKIVNP IMGVAFWRDD VKIDKEAVTV RFEEGRPVAL NGVEYKDAVE LLLEANRIGG RHGLGMSDQI ENRIIEAKSR GIYEAPGLAL LYIAYERLVT GIHNEDTIEQ YRENGRRLGR LLYQGRWFDP QAIMLRETAQ RWVARAVTGE VTVELRRGND YSILATRSPN LTYQPERLSM EKVQSTFSPR DRIGQLTMRN LDITDTRDKL RIYSEVGLLT PGESSALPKL KEDESGN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCACGATT CTTGAAAATC TCCCGGCCGG CCAGAAAGTC GGCATCGCGT TCTCCGGCGG GCTCGACACG AGCGCCGCGC TGCACTGGAT GCGCATCAAG GGGGCCGTTC CTTACGCGTA CACGGCCAAT CTCGGCCAGC CCGACGAAGA CGACTACGAT GCGATCCCGA AGCGCGCGAT CCAGTACGGC GCCGAGGGCG CACGCCTGAT TGACTGCCGC GCGCAGCTCG TGGCCGAAGG GATCGCGGCG CTCCAGTGCG GCGCGTTCCA CATCTCGACG GCCGGCGTCA CGTATTTCAA TACGACCCCG ATTGGCCGCG CCGTGACGGG CACGATGCTC GTCGCCGCGA TGAAGGAGGA CGGCGTCAAC ATCTGGGGCG ACGGCAGCAC GTACAAGGGC AACGACATCG AGCGCTTCTA CCGCTACGGC CTGCTCGTCA ATCCGGATCT GAAGATCTAC AAGCCGTGGC TCGATCAGCA GTTCATCGAC GAGCTGGGCG GCCGCGCGGA AATGTCCGAA TTCATGCGCC AGGCCGGCTT CGAGTACAAG ATGTCGGCGG AGAAGGCGTA TTCGACCGAT TCGAACCTGC TGGGCGCGAC GCACGAGGCG AAGGATCTCG AGAGCCTCGA GAGCGGCATC AAGATCGTCA ATCCGATCAT GGGCGTCGCG TTCTGGCGCG ACGACGTGAA GATCGACAAG GAAGCGGTGA CGGTCCGCTT CGAAGAGGGC CGCCCCGTCG CGCTGAACGG CGTCGAGTAC AAGGATGCGG TCGAGCTGCT GCTCGAGGCG AACCGGATCG GCGGCCGCCA CGGCCTCGGG ATGAGCGACC AGATCGAGAA CCGGATCATC GAGGCGAAGA GCCGCGGCAT CTATGAAGCC CCGGGGCTTG CACTGCTGTA CATCGCGTAC GAGCGCCTCG TCACCGGCAT CCACAACGAA GACACGATCG AGCAGTACCG CGAGAACGGC CGCCGCCTCG GCCGCCTGCT GTACCAGGGC CGCTGGTTCG ATCCGCAGGC GATCATGCTG CGCGAGACCG CGCAGCGCTG GGTCGCGCGC GCGGTCACGG GCGAGGTGAC GGTCGAGCTG CGCCGCGGCA ACGACTATTC GATTCTCGCC ACGCGCTCGC CGAACCTGAC GTACCAGCCG GAGCGCCTGT CGATGGAGAA GGTGCAGTCG ACGTTCTCGC CGCGCGACCG GATCGGCCAG TTGACGATGC GCAACCTCGA CATCACCGAC ACCCGCGACA AGCTGCGCAT CTACTCGGAA GTCGGCCTGC TCACGCCGGG CGAATCGTCG GCGCTGCCGA AGCTGAAGGA AGACGAGAGC GGCAACTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA