ButhA.00725.a

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (CMK) (EC 2.7.1.148) (4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase) (BTH_I0476) (ispE)

CENTER ID: ButhA.00725.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00725.a.A1.GE32746 full length 1 293
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00725.a.A1.PW37639 full length 1 293

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.3155
RefSeq: YP_441033.1
UniProt: Q2T1B6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDTTRSLRD CLAPAKLNLF LHITGRRPDG YHELQSVFQL LDWGDRLHFT LRDDGKVSRK TDVPGVPEET DLIVRAASLL KAHTGTAAGV DIEIDKRLPM GAGLGGGSSD AATTLLALNR LWKLDLPRAT LQSLAVKLGA DVPFFVFGKN AFAEGIGEAL QAVELPTRWF LVVTPRVHVP TAAIFSEKSL TRDSKPITIT DFLAQQDCNT GWPDSFGRND MQPVVTSKYA EVAKVVGWFY NLTPARMTGS GASVFAAFKS KAEAGAAQAQ LPAGWDSAVA ESLGEHPLFA FAS
NT Sequence
atgaccgata cgacccgctc gctgcgcgac tgcctcgccc cggcgaaact gaacctgttc ctgcacatca cgggccgtcg tccggacggc tatcacgagc tgcaaagcgt gttccagctg ctcgactggg gcgaccggct gcacttcacg ctgcgcgacg acggcaaggt gtcgcgcaag accgacgtgc cgggcgtacc cgaggaaacc gacctcatcg tgcgcgcggc gtcgctgctg aaagcgcaca cgggcacggc ggcgggcgtc gacatcgaga tcgacaagcg actgccgatg ggcgcgggcc tcggcggagg cagctcggat gcggcgacga cgttgctcgc gctcaaccgc ctctggaagc tcgacttgcc gcgcgccacg ctgcaatcgc tcgcggtgaa gctcggcgcc gacgtgccgt tcttcgtctt cggaaaaaat gcgttcgcag agggtatcgg agaagcgctg caagctgtag aattgccgac tcgctggttt ctggttgtga caccgcgggt tcacgttccg accgcagcga ttttttccga aaaatcgttg acaagagatt cgaaacccat cacaattacg gactttcttg cacagcaaga ctgcaacacg ggatggcctg acagtttcgg tcggaatgac atgcagccgg ttgtgacaag caagtacgcg gaagttgcaa aggtggtcgg atggttttat aatctgaccc ccgcgcggat gaccggctcc ggagctagcg tgtttgcagc gttcaagagc aaggcggagg caggagcggc gcaagcccaa ctgccggccg gctgggacag cgcagttgcc gagagcttgg gtgagcatcc actcttcgct ttcgcgtcat aa
Details for ButhA.00725.a.A1.GE32746
HARVESTED ON: 6/20/2011
SEQUENCED ON: 5/1/2015
EXPECTED MW: 33kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMTDTTRS LRDCLAPAKL NLFLHITGRR PDGYHELQSV FQLLDWGDRL HFTLRDDGKV SRKTDVPGVP EETDLIVRAA SLLKAHTGTA AGVDIEIDKR LPMGAGLGGG SSDAATTLLA LNRLWKLDLP RATLQSLAVK LGADVPFFVF GKNAFAEGIG EALQAVELPT RWFLVVTPRV HVPTAAIFSE KSLTRDSKPI TITDFLAQQD CNTGWPDSFG RNDMQPVVTS KYAEVAKVVG WFYNLTPARM TGSGASVFAA FKSKAEAGAA QAQLPAGWDS AVAESLGEHP LFAFAS
Validated NT Sequence
tattatgacg cgaaagcgaa gagtggatgc tcacccaagc tctcggcaac tgcgctgtcc cagccggccg gcagttgggc ttgcgccgct cctgcctccg ccttgctctt gaacgctgca aacacgctag ctccggagcc ggtcatccgc gcgggggtca gattataaaa ccatccgacc acctttgcaa cttccgcgta cttgcttgtc acaaccggct gcatgtcatt ccgaccgaaa ctgtcaggcc atcccgtgtt gcagtcttgc tgtgcaagaa agtccgtaat tgtgatgggt ttcgaatctc ttgtcaacga tttttcggaa aaaatcgctg cggtcggaac gtgaacccgc ggtgtcacaa ccagaaacca gcgagtcggc aattctacag cttgcagcgc ttctccgata ccctctgcga acgcattttt tccgaagacg aagaacggca cgtcggcgcc gagcttcacc gcgagcgatt gcagcgtggc gcgcggcaag tcgagcttcc agaggcggtt gagcgcgagc aacgtcgtcg ccgcatccga gctgcctccg ccgaggcccg cgcccatcgg cagtcgcttg tcgatctcga tgtcgacgcc cgccgccgtg cccgtgtgcg ctttcagcag cgacgccgcg cgcacgatga ggtcggtttc ctcgggtacg cccggcacgt cggtcttgcg cgacaccttg ccgtcgtcgc gcagcgtgaa gtgcagccgg tcgccccagt cgagcagctg gaacacgctt tgcagctcgt gatagccgtc cggacgacgg cccgtgatgt gcaggaacag gttcagtttc gccggggcga ggcagtcgcg cagcgagcgg gtcgtatcgg tcatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagcca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDTTRSLR DCLAPAKLNL FLHITGRRPD GYHELQSVFQ LLDWGDRLHF TLRDDGKVSR KTDVPGVPEE TDLIVRAASL LKAHTGTAAG VDIEIDKRLP MGAGLGGGSS DAATTLLALN RLWKLDLPRA TLQSLAVKLG ADVPFFVFGK NAFAEGIGEA LQAVELPTRW FLVVTPRVHV PTAAIFSEKS LTRDSKPITI TDFLAQQDCN TGWPDSFGRN DMQPVVTSKY AEVAKVVGWF YNLTPARMTG SGASVFAAFK SKAEAGAAQA QLPAGWDSAV AESLGEHPLF AFAS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgatacg acccgctcgc tgcgcgactg cctcgccccg gcgaaactga acctgttcct gcacatcacg ggccgtcgtc cggacggcta tcacgagctg caaagcgtgt tccagctgct cgactggggc gaccggctgc acttcacgct gcgcgacgac ggcaaggtgt cgcgcaagac cgacgtgccg ggcgtacccg aggaaaccga cctcatcgtg cgcgcggcgt cgctgctgaa agcgcacacg ggcacggcgg cgggcgtcga catcgagatc gacaagcgac tgccgatggg cgcgggcctc ggcggaggca gctcggatgc ggcgacgacg ttgctcgcgc tcaaccgcct ctggaagctc gacttgccgc gcgccacgct gcaatcgctc gcggtgaagc tcggcgccga cgtgccgttc ttcgtcttcg gaaaaaatgc gttcgcagag ggtatcggag aagcgctgca agctgtagaa ttgccgactc gctggtttct ggttgtgaca ccgcgggttc acgttccgac cgcagcgatt ttttccgaaa aatcgttgac aagagattcg aaacccatca caattacgga ctttcttgca cagcaagact gcaacacggg atggcctgac agtttcggtc ggaatgacat gcagccggtt gtgacaagca agtacgcgga agttgcaaag gtggtcggat ggttttataa tctgaccccc gcgcggatga ccggctccgg agctagcgtg tttgcagcgt tcaagagcaa ggcggaggca ggagcggcgc aagcccaact gccggccggc tgggacagcg cagttgccga gagcttgggt gagcatccac tcttcgcttt cgcgtcataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for ButhA.00725.a.A1.PW37639
PURIFICATION DATe: 1/26/2015
CONCENTRATION: 1.7mg/ml
OBSERVED MW: 34kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 25000µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMTDTTRS LRDCLAPAKL NLFLHITGRR PDGYHELQSV FQLLDWGDRL HFTLRDDGKV SRKTDVPGVP EETDLIVRAA SLLKAHTGTA AGVDIEIDKR LPMGAGLGGG SSDAATTLLA LNRLWKLDLP RATLQSLAVK LGADVPFFVF GKNAFAEGIG EALQAVELPT RWFLVVTPRV HVPTAAIFSE KSLTRDSKPI TITDFLAQQD CNTGWPDSFG RNDMQPVVTS KYAEVAKVVG WFYNLTPARM TGSGASVFAA FKSKAEAGAA QAQLPAGWDS AVAESLGEHP LFAFAS
Validated NT Sequence
tattatgacg cgaaagcgaa gagtggatgc tcacccaagc tctcggcaac tgcgctgtcc cagccggccg gcagttgggc ttgcgccgct cctgcctccg ccttgctctt gaacgctgca aacacgctag ctccggagcc ggtcatccgc gcgggggtca gattataaaa ccatccgacc acctttgcaa cttccgcgta cttgcttgtc acaaccggct gcatgtcatt ccgaccgaaa ctgtcaggcc atcccgtgtt gcagtcttgc tgtgcaagaa agtccgtaat tgtgatgggt ttcgaatctc ttgtcaacga tttttcggaa aaaatcgctg cggtcggaac gtgaacccgc ggtgtcacaa ccagaaacca gcgagtcggc aattctacag cttgcagcgc ttctccgata ccctctgcga acgcattttt tccgaagacg aagaacggca cgtcggcgcc gagcttcacc gcgagcgatt gcagcgtggc gcgcggcaag tcgagcttcc agaggcggtt gagcgcgagc aacgtcgtcg ccgcatccga gctgcctccg ccgaggcccg cgcccatcgg cagtcgcttg tcgatctcga tgtcgacgcc cgccgccgtg cccgtgtgcg ctttcagcag cgacgccgcg cgcacgatga ggtcggtttc ctcgggtacg cccggcacgt cggtcttgcg cgacaccttg ccgtcgtcgc gcagcgtgaa gtgcagccgg tcgccccagt cgagcagctg gaacacgctt tgcagctcgt gatagccgtc cggacgacgg cccgtgatgt gcaggaacag gttcagtttc gccggggcga ggcagtcgcg cagcgagcgg gtcgtatcgg tcatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagcca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDTTRSLR DCLAPAKLNL FLHITGRRPD GYHELQSVFQ LLDWGDRLHF TLRDDGKVSR KTDVPGVPEE TDLIVRAASL LKAHTGTAAG VDIEIDKRLP MGAGLGGGSS DAATTLLALN RLWKLDLPRA TLQSLAVKLG ADVPFFVFGK NAFAEGIGEA LQAVELPTRW FLVVTPRVHV PTAAIFSEKS LTRDSKPITI TDFLAQQDCN TGWPDSFGRN DMQPVVTSKY AEVAKVVGWF YNLTPARMTG SGASVFAAFK SKAEAGAAQA QLPAGWDSAV AESLGEHPLF AFAS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGATACG ACCCGCTCGC TGCGCGACTG CCTCGCCCCG GCGAAACTGA ACCTGTTCCT GCACATCACG GGCCGTCGTC CGGACGGCTA TCACGAGCTG CAAAGCGTGT TCCAGCTGCT CGACTGGGGC GACCGGCTGC ACTTCACGCT GCGCGACGAC GGCAAGGTGT CGCGCAAGAC CGACGTGCCG GGCGTACCCG AGGAAACCGA CCTCATCGTG CGCGCGGCGT CGCTGCTGAA AGCGCACACG GGCACGGCGG CGGGCGTCGA CATCGAGATC GACAAGCGAC TGCCGATGGG CGCGGGCCTC GGCGGAGGCA GCTCGGATGC GGCGACGACG TTGCTCGCGC TCAACCGCCT CTGGAAGCTC GACTTGCCGC GCGCCACGCT GCAATCGCTC GCGGTGAAGC TCGGCGCCGA CGTGCCGTTC TTCGTCTTCG GAAAAAATGC GTTCGCAGAG GGTATCGGAG AAGCGCTGCA AGCTGTAGAA TTGCCGACTC GCTGGTTTCT GGTTGTGACA CCGCGGGTTC ACGTTCCGAC CGCAGCGATT TTTTCCGAAA AATCGTTGAC AAGAGATTCG AAACCCATCA CAATTACGGA CTTTCTTGCA CAGCAAGACT GCAACACGGG ATGGCCTGAC AGTTTCGGTC GGAATGACAT GCAGCCGGTT GTGACAAGCA AGTACGCGGA AGTTGCAAAG GTGGTCGGAT GGTTTTATAA TCTGACCCCC GCGCGGATGA CCGGCTCCGG AGCTAGCGTG TTTGCAGCGT TCAAGAGCAA GGCGGAGGCA GGAGCGGCGC AAGCCCAACT GCCGGCCGGC TGGGACAGCG CAGTTGCCGA GAGCTTGGGT GAGCATCCAC TCTTCGCTTT CGCGTCATAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA