ButhA.00719.a
Agmatinase, putative (EC 3.5.3.11) (BTH_II1941)
| CENTER ID: | ButhA.00719.a |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00719.a.A1.GE32745 | full length | 1 | 320 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
4DZ4
DEPOSITED: 2/29/2012
DETERMINATION: XRay
CLONE: ButhA.00719.a.A1.GE32745
PROTEIN: ButhA.00719.a.A1.PS01293
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127636 |
| PATRIC ID: | fig|271848.6.peg.2160 |
| RefSeq: | YP_440135.1 |
| UniProt: | Q2T3W4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNETLYGDGA IRRPSVYGSS IENTYAGVLS FMRRNYTRDL DGVDVVVSGV PLDLATTFRS GARLGPSAVR AASVQLAELN PYPWGFDPFD DLAVIDYGDC WFDAHHPLSI KPAIVEHART ILQSDARMLT LGGDHYITYP LLIAHAQKYG KPLSLIHFDA HCDTWADDAP DSLNHGTMFY KAVKDGLIDP KASVQVGIRT WNDDYLGINV LDAAWVHEHG ARATLERIES IVGGRPAYLT FDIDCLDPAF APGTGTPVAG GLSSAQALAI VRGLGGVNLI GADVVEVAPA YDQSEITAIA AAHVACDLLC LWRQRKAGAR
NT Sequence
atgaatgaaa cgctttatgg cgacggcgcg atccgccgtc catccgtcta cggttcctcg atcgaaaaca cttacgcggg cgtgctgtcg ttcatgcggc gcaactacac gcgcgatctc gacggcgtcg acgtcgtcgt gtccggcgtg ccgctcgatc tcgcgacgac gtttcgctcc ggcgcgcgcc tgggcccgtc cgcggtgcgc gcggcgagcg tgcagcttgc cgagctgaat ccgtatccct ggggattcga tccgttcgac gatctcgcgg tgatcgacta cggcgactgc tggttcgacg cgcaccatcc gctctcgatc aagcccgcga tcgtcgagca cgcgcggacg atcctgcaat cggacgcgag gatgctgacg ctcggcggcg accactacat cacgtatccg ttgctgatcg cgcatgcgca gaagtacggc aagccgctgt cgctgatcca tttcgacgcg cactgcgaca cgtgggccga cgacgcgccc gacagcctca atcacggcac gatgttctac aaggccgtca aagacgggct gatcgatccg aaggcgtcgg tgcaggtcgg catccgcact tggaacgacg actatctcgg catcaacgtg ctcgacgccg cctgggtgca cgagcacggc gcgcgcgcga cgctcgagcg catcgagtcg atcgtcggcg gccggcctgc ctatctgacg ttcgacatcg actgcctcga tcccgcgttc gcgccgggaa cgggcacgcc ggtcgcgggc gggctgtcgt ccgcgcaggc gctcgcgatc gtgcgcggtc tcggcggcgt gaacctgatc ggcgcggacg tcgttgaggt cgcgcccgct tacgaccaga gcgagatcac cgcgatcgcg gccgcgcatg tcgcatgcga tctgctgtgt ctgtggcggc agcgcaaggc gggcgcgcgc tga
Details for ButhA.00719.a.A1.GE32745
| HARVESTED ON: | 6/20/2011 |
| SEQUENCED ON: | 7/14/2011 |
| EXPECTED MW: | 36kDa |
| OBSERVED MW: | 36kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MGTLEAQTQG PGSMNETLYG DGAIRRPSVY GSSIENTYAG VLSFMRRNYT RDLDGVDVVV SGVPLDLATT FRSGARLGPS AVRAASVQLA ELNPYPWGFD PFDDLAVIDY GDCWFDAHHP LSIKPAIVEH ARTILQSDAR MLTLGGDHYI TYPLLIAHAQ KYGKPLSLIH FDAHCDTWAD DAPDSLNHGT MFYKAVKDGL IDPKASVQVG IRTWNDDYLG INVLDAAWVH EHGARATLER IESIVGGRPA YLTFDIDCLD PAFAPGTGTP VAGGLSSAQA LAIVRGLGGV NLIGADVVEV APAYDQSEIT AIAAAHVACD LLCLWRQRKA GAR
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgaatga aacgctttat ggcgacggcg cgatccgccg tccatccgtc tacggttcct cgatcgaaaa cacttacgcg ggcgtgctgt cgttcatgcg gcgcaactac acgcgcgatc tcgacggcgt cgacgtcgtc gtgtccggcg tgccgctcga tctcgcgacg acgtttcgct ccggcgcgcg cctgggcccg tccgcggtgc gcgcggcgag cgtgcagctt gccgagctga atccgtatcc ctggggattc gatccgttcg acgatctcgc ggtgatcgac tacggcgact gctggttcga cgcgcaccat ccgctctcga tcaagcccgc gatcgtcgag cacgcgcgga cgatcctgca atcggacgcg aggatgctga cgctcggcgg cgaccactac atcacgtatc cgttgctgat cgcgcatgcg cagaagtacg gcaagccgct gtcgctgatc catttcgacg cgcactgcga cacgtgggcc gacgacgcgc ccgacagcct caatcacggc acgatgttct acaaggccgt caaagacggg ctgatcgatc cgaaggcgtc ggtgcaggtc ggcatccgca cttggaacga cgactatctc ggcatcaacg tgctcgacgc cgcctgggtg cacgagcacg gcgcgcgcgc gacgctcgag cgcatcgagt cgatcgtcgg cggccggcct gcctatctga cgttcgacat cgactgcctc gatcccgcgt tcgcgccggg aacgggcacg ccggtcgcgg gcgggctgtc gtccgcgcag gcgctcgcga tcgtgcgcgg tctcggcggc gtgaacctga tcggcgcgga cgtcgttgag gtcgcgcccg cttacgacca gagcgagatc accgcgatcg cggccgcgca tgtcgcatgc gatctgctgt gtctgtggcg gcagcgcaag gcgggcgcgc gctaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNETLYGDG AIRRPSVYGS SIENTYAGVL SFMRRNYTRD LDGVDVVVSG VPLDLATTFR SGARLGPSAV RAASVQLAEL NPYPWGFDPF DDLAVIDYGD CWFDAHHPLS IKPAIVEHAR TILQSDARML TLGGDHYITY PLLIAHAQKY GKPLSLIHFD AHCDTWADDA PDSLNHGTMF YKAVKDGLID PKASVQVGIR TWNDDYLGIN VLDAAWVHEH GARATLERIE SIVGGRPAYL TFDIDCLDPA FAPGTGTPVA GGLSSAQALA IVRGLGGVNL IGADVVEVAP AYDQSEITAI AAAHVACDLL CLWRQRKAGA R
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatgaaacg ctttatggcg acggcgcgat ccgccgtcca tccgtctacg gttcctcgat cgaaaacact tacgcgggcg tgctgtcgtt catgcggcgc aactacacgc gcgatctcga cggcgtcgac gtcgtcgtgt ccggcgtgcc gctcgatctc gcgacgacgt ttcgctccgg cgcgcgcctg ggcccgtccg cggtgcgcgc ggcgagcgtg cagcttgccg agctgaatcc gtatccctgg ggattcgatc cgttcgacga tctcgcggtg atcgactacg gcgactgctg gttcgacgcg caccatccgc tctcgatcaa gcccgcgatc gtcgagcacg cgcggacgat cctgcaatcg gacgcgagga tgctgacgct cggcggcgac cactacatca cgtatccgtt gctgatcgcg catgcgcaga agtacggcaa gccgctgtcg ctgatccatt tcgacgcgca ctgcgacacg tgggccgacg acgcgcccga cagcctcaat cacggcacga tgttctacaa ggccgtcaaa gacgggctga tcgatccgaa ggcgtcggtg caggtcggca tccgcacttg gaacgacgac tatctcggca tcaacgtgct cgacgccgcc tgggtgcacg agcacggcgc gcgcgcgacg ctcgagcgca tcgagtcgat cgtcggcggc cggcctgcct atctgacgtt cgacatcgac tgcctcgatc ccgcgttcgc gccgggaacg ggcacgccgg tcgcgggcgg gctgtcgtcc gcgcaggcgc tcgcgatcgt gcgcggtctc ggcggcgtga acctgatcgg cgcggacgtc gttgaggtcg cgcccgctta cgaccagagc gagatcaccg cgatcgcggc cgcgcatgtc gcatgcgatc tgctgtgtct gtggcggcag cgcaaggcgg gcgcgcgcta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a