ButhA.00630.a

Rod shape-determining protein MreB (BTH_I0146)

CENTER ID: ButhA.00630.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00630.a.A1.GE32799 full length 1 347
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.2789
RefSeq: YP_440704.1
UniProt: Q2T295

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFGFLRSYFS NDLAIDLGTA NTLIYMRGKG IVLDEPSVVS IRQEGGPNGK KTIQAVGKEA KQMLGKVPGN IEAIRPMKDG VIADFTVTEQ MIKQFIKTAH ESRMFSPSPR IIICVPCGST QVERRAIKEA AHGAGASQVY LIEEPMAAAI GAGLPVSEAT GSMVVDIGGG TTEVGVISLG GIVYKGSVRV GGDKFDEAIV NYIRRNYGML IGEQTAEAIK KEIGSAFPGS EVKEMEVKGR NLSEGIPRSF TISSNEILEA LTDPLNQIVS SVKIALEQTP PELGADIAER GMMLTGGGAL LRDLDRLLAE ETGLPVLVAE DPLTCVVRGS GMALERMDKL GSIFSYE
NT Sequence
atgttcggtt ttttgcgcag ctatttctcc aacgatctcg cgatcgatct cggcaccgca aacaccctga tctacatgcg cggcaagggc atcgtgctcg atgagccgtc cgtcgtctcg atccgccagg aaggcggccc gaacggcaag aaaacgattc aagcagtggg caaggaagcg aagcagatgc tcggcaaggt gcccggcaac atcgaggcga tccgtccgat gaaagacggc gtgatcgccg atttcacggt caccgagcag atgatcaagc agttcatcaa gactgcgcac gaatcgcgga tgttctcgcc gtcgccgcgc atcatcatct gcgtgccgtg cggctcgacc caggtcgagc gccgcgcgat caaggaggcg gcccacggcg cgggcgcgtc gcaggtctac ctgatcgagg agccgatggc ggccgcgatc ggcgcgggcc tgccggtgtc ggaggcgacg ggctcgatgg tcgtcgacat cggcggcggc acgacggaag tcggcgtgat ctcgctcggc ggcatcgtgt acaagggttc ggtgcgcgtc ggcggcgaca agttcgacga ggcgatcgtc aactacatcc gccgcaacta cgggatgctg atcggcgagc agacggccga ggcgatcaag aaggagatcg gctccgcgtt cccgggctcc gaagtcaagg aaatggaagt gaagggccgc aacctgtcgg aaggcattcc gcgcagcttc acgatctcca gcaacgaaat cctcgaggcc ctgaccgatc cgctgaacca gatcgtgtca tcggtgaaaa tcgcgctcga gcagacgccg ccggaactcg gcgcggacat cgccgagcgc ggcatgatgc tcacgggcgg cggcgcgctg ctgcgcgacc tcgatcgcct gctcgccgag gaaaccggcc tgcccgtgct cgtcgccgag gacccgctca cctgcgtcgt gcgcggctcc gggatggcgc tcgaacggat ggacaagctc ggcagcatct tctcgtacga gtga
Details for ButhA.00630.a.A1.GE32799
HARVESTED ON: 6/28/2011
SEQUENCED ON: 7/22/2011
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 82
PERCENT COVERAGE: 104
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFGFLRSYF SNDLAIDLGT ANTLIYMRGK GIVLDEPSVV SIRQEGGPNG KKTIQAVGKE AKQMLGKVPG NIEAIRPMKD GVIADFTVTE QMIKQFIKTA HESRMFSPSP RIIICVPCGS TQVERRAIKE AAHGAGASQV YLIEEPMAAA IGAGLPVSEA TGSMVVDIGG GTTEVGVISL GGIVYKGSVR VGGDKFDEAI VNYIRRNYGM LIGEQTAXAI KKEIGSAFPG SEVKEMEVKG RNLSEGIPRS FTISSNEILE ALTDPLNQIX SSVKIALEQT PPXLXAXXXX RXXXSRAXXA AARLXXXSXX XXXRARXXTX XXXXXXXXXX XXXXXXXXXX YXXXXXXXXX XXXXXXXXXX XXX
Validated NT Sequence
tttgtttnnc tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgttcgg ttttttgcgc agctatttct ccaacgatct cgcgatcgat ctcggcaccg caaacaccct gatctacatg cgcggcaagg gcatcgtgct cgatgagccg tccgtcgtct cgatccgcca ggaaggcggc ccgaacggca agaaaacgat tcaagcagtg ggcaaggaag cgaagcagat gctcggcaag gtgcccggca acatcgaggc gatccgtccg atgaaagacg gcgtgatcgc cgatttcacg gtcaccgagc agatgatcaa gcagttcatc aagactgcgc acgaatcgcg gatgttctcg ccgtcgccgc gcatcatcat ctgcgtgccg tgcggctcga cccaggtcga gcgccgcgcg atcaaggagg cggcccacgg cgcgggcgcg tcgcaggtct acctgatcga ggagccgatg gcggccgcga tcggcgcggg cctgccggtg tcggaggcga cgggctcgat ggtcgtcgac atcggcggcg gcacgacgga agtcggcgtg atctcgctcg gcggcatcgt gtacaagggt tcggtgcgcg tcggcggcga caagttcgac gaggcgatcg tcaactacat ccgccgcaac tacgggatgc tgatcggcga gcagacggcc gangcgatca agaaggagat cggctccgcg ttcccgggct ccgaagtcaa ggaaatggaa gtgaagggcc gcaacctgtc ggaaggcatt ccgcgcagct tcacgatctc cagcaacgaa atcctcgagg ccctgaccga tccgctgaac cagatcgngt catcggtgaa aatcgcgctc gagcagacgc cgccnnaact cngcgcggan ntcgncnagc gngnatgntg ntcacgggcg gngngcgctg ctgcgcgact cnatcnnngc tcncnannan cnnnngnccg tgctcgncnc nagaccnctn ncngnntcnn gnnnngntnn ngnngnangn ngnnanntnn nnncatnnnn ncgnncnant atnngcannn nnnnnnnngn nnnnnnnnnn nnnnnnnnnn nnnnnannnn nnnnnnnnnn nnnnnnngnn t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFGFLRSYF SNDLAIDLGT ANTLIYMRGK GIVLDEPSVV SIRQEGGPNG KKTIQAVGKE AKQMLGKVPG NIEAIRPMKD GVIADFTVTE QMIKQFIKTA HESRMFSPSP RIIICVPCGS TQVERRAIKE AAHGAGASQV YLIEEPMAAA IGAGLPVSEA TGSMVVDIGG GTTEVGVISL GGIVYKGSVR VGGDKFDEAI VNYIRRNYGM LIGEQTAEAI KKEIGSAFPG SEVKEMEVKG RNLSEGIPRS FTISSNEILE ALTDPLNQIV SSVKIALEQT PPELGADIAE RGMMLTGGGA LLRDLDRLLA EETGLPVLVA EDPLTCVVRG SGMALERMDK LGSIFSYE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttcggtttt ttgcgcagct atttctccaa cgatctcgcg atcgatctcg gcaccgcaaa caccctgatc tacatgcgcg gcaagggcat cgtgctcgat gagccgtccg tcgtctcgat ccgccaggaa ggcggcccga acggcaagaa aacgattcaa gcagtgggca aggaagcgaa gcagatgctc ggcaaggtgc ccggcaacat cgaggcgatc cgtccgatga aagacggcgt gatcgccgat ttcacggtca ccgagcagat gatcaagcag ttcatcaaga ctgcgcacga atcgcggatg ttctcgccgt cgccgcgcat catcatctgc gtgccgtgcg gctcgaccca ggtcgagcgc cgcgcgatca aggaggcggc ccacggcgcg ggcgcgtcgc aggtctacct gatcgaggag ccgatggcgg ccgcgatcgg cgcgggcctg ccggtgtcgg aggcgacggg ctcgatggtc gtcgacatcg gcggcggcac gacggaagtc ggcgtgatct cgctcggcgg catcgtgtac aagggttcgg tgcgcgtcgg cggcgacaag ttcgacgagg cgatcgtcaa ctacatccgc cgcaactacg ggatgctgat cggcgagcag acggccgagg cgatcaagaa ggagatcggc tccgcgttcc cgggctccga agtcaaggaa atggaagtga agggccgcaa cctgtcggaa ggcattccgc gcagcttcac gatctccagc aacgaaatcc tcgaggccct gaccgatccg ctgaaccaga tcgtgtcatc ggtgaaaatc gcgctcgagc agacgccgcc ggaactcggc gcggacatcg ccgagcgcgg catgatgctc acgggcggcg gcgcgctgct gcgcgacctc gatcgcctgc tcgccgagga aaccggcctg cccgtgctcg tcgccgagga cccgctcacc tgcgtcgtgc gcggctccgg gatggcgctc gaacggatgg acaagctcgg cagcatcttc tcgtacgagt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa