ButhA.00612.a
Lysyl-tRNA synthetase (EC 6.1.1.6) (Lysine--tRNA ligase) (LysRS) (BTH_I1883) (lysS)
| CENTER ID: | ButhA.00612.a |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00612.a.A1.GE32798 | full length | 1 | 508 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
4EX5
DEPOSITED: 4/29/2012
DETERMINATION: XRay
CLONE: ButhA.00612.a.A1.GE32798
PROTEIN: ButhA.00612.a.A1.PS01208
Publications by SSGCID
Ligand co-crystallization of aminoacyl-tRNA synthetases from infectious disease organisms
Clifton MC, Dranow DM, Edwards TE, Lorimer DD, Manoil C, Moen S, Myler PJ, Sankaran B, Sharma A, Staker BL, Van Voorhis WC
Sci Rep - 2017
volume 7, issue 1, pages 223
PMID: 28303005; PMCID: PMC5428304
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|271848.6.peg.4690 |
| RefSeq: | YP_442413.1 |
| UniProt: | Q2SXD6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTEPIQPQAA VAADENQIVA ERRDKLRALR DQGIAYPNDF QPTHHAADLQ TAYADADKEA LEAKSLEVAI AGRMMLKRVM GKASFATVQD GSGQIQFFVT PADVGAETYD AFKKWDLGDI VAARGVLFRT NKGELSVKCT QLRLLAKALR PLPDKFHGLA DQETRYRQRY VDLIVTPETR TTFRARTKAI ASIRKFMGDA DFMEVETPML HPIPGGAAAK PFVTHHNALD MEMFLRIAPE LYLKRLIVGG FERVFEINRN FRNEGVSPRH NPEFTMMEFY AAYTDYRWLM DFTERLIRQA AVDALGTATI QYQGRELDLA QPFHRLTITQ AIQKYAPSYT DGQLSDDAFL RSELKRLGVD VTQPAFLNAG IGALQLALFE ETAEAQLWEP TFIIDYPIEV SPLARESDTV AGITERFELF ITGREIANGF SELNDPEDQA ARFKKQVEQK DAGDEEAMFF DADYIRALEY GMPPTGGCGI GIDRLVMLLT DSPTIRDVLL FPHLRRED
NT Sequence
atgaccgaac cgatccaacc gcaggccgct gtcgcagcgg acgaaaacca gatcgtcgcg gaacgccgcg acaagctgcg cgcgctgcgc gaccaaggca tcgcctatcc gaacgacttc cagccgacgc accacgcggc cgatctgcag accgcatacg cggacgccga caaggaagcg ctcgaggcga agtcgctcga ggtcgcgatc gccggccgga tgatgctcaa gcgcgtgatg ggcaaggcga gcttcgcgac ggtgcaggac ggctcgggtc agatccagtt cttcgtgacg cccgccgacg tcggcgcgga aacgtacgat gcgttcaaga agtgggacct gggcgacatc gtcgccgcgc gcggcgtgct gtttcgcacg aacaagggcg agctgtcggt gaagtgcacg cagctgcggc tgctcgcgaa ggcgctgcgc ccgctgcccg acaagttcca cggcctcgct gaccaggaaa cgcgctatcg ccagcgctac gtcgacctga tcgtcacgcc cgagacgcgc acgacgtttc gcgcgcgcac gaaggcgatc gcgtcgatcc gcaagttcat gggcgacgcc gatttcatgg aagtcgagac gccgatgctg cacccgatcc cgggcggcgc ggccgcgaag ccgttcgtca cgcatcacaa cgcgctcgac atggaaatgt tcctgcgcat cgcgcccgag ctgtacctga agcgcctcat cgtcggcggc ttcgagcgcg tgttcgagat caaccggaat ttccgcaacg agggcgtgtc gccgcgccac aatccggaat tcacgatgat ggagttctac gccgcgtaca ccgactaccg ctggctgatg gacttcaccg agcggctgat ccgccaggcg gccgtggacg cgctcggcac cgcgacgatc cagtaccagg gccgcgagct cgatctcgcg caaccgtttc atcgcctgac gatcacgcag gcgatccaga agtacgcgcc cagctacacg gatggccaac tgtcggacga cgcgttcctg cgcagcgaac tgaagcgcct cggcgtcgac gtcacgcagc ccgcgttcct gaacgccggc atcggcgcgc tgcagctcgc cctcttcgag gaaaccgcgg aagcgcaact gtgggagccg accttcatca tcgactaccc gatcgaagtg tcgccgctcg cgcgcgaatc ggacacggtg gccggcatca ccgagcgttt cgagctcttc atcaccggcc gcgaaatcgc gaacgggttc tcggagctga acgatcccga agaccaggcc gcgcgcttca agaagcaggt cgaacagaag gacgcgggcg acgaggaagc gatgttcttc gatgcggact acatccgcgc gctcgaatac gggatgcctc cgacaggcgg ctgcgggatc ggcatcgacc gtctcgtgat gctgctgacc gacagcccga cgatccgcga cgtgctgctg ttcccgcacc tgcgtcgcga ggactga
Details for ButhA.00612.a.A1.GE32798
| HARVESTED ON: | 6/28/2011 |
| SEQUENCED ON: | 7/22/2011 |
| EXPECTED MW: | 59kDa |
| OBSERVED MW: | 64kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 95 |
Validated AA Sequence
MTEPIQPQAA VAADENQIVA ERRDKLRALR DQGIAYPNDF QPTHHAADLQ TAYADADKEA LEAKSLEVAI AGRMMLKRVM GKASFATVQD GSGQIQFFVT PADVGAETYD AFKKWDLGDI VAARGVLFRT NKGELSVKCT QLRLLAKALR PLPDKFHGLA DQETRYRQRY VDLIVTPETR TTFRARTKAI ASIRKFMGDA XFMEVETPML HPIPGGAAAK PFVTHHNALD MEMFLRIAPE LYLKRLIVGG FERVFEINRN FRNEGVSPRH NPEFTMMEFY AAYTDYRWLM DFTERLIRQA AVDALGTATI QYQGRELDLA QPFHRLTITQ AIQKYAPSYT DGQLSDDAFL RSELKRLGVD VTQPAFLNAG IGALQLALFE ETAEAQLWEP TFIIDYPIEV SPLARESDTV AGITERFELF ITGREIANGF SELNDPEDQA ARFKKQVEQK DAGDEEAMFF DADYIRALEY GMPPTGGCGI GIDRLVMLLT DSPTIRDVLL FPHLRR
Validated NT Sequence
cgcgacgcag gtgcgggaac agcagcacgt cgcggatcgt cgggctgtcg gtcagcagca tcacgagacg gtcgatgccg atcccgcagc cgcctgtcgg aggcatcccg tattcgagcg cgcggatgta gtccgcatcg aagaacatcg cttcctcgtc gcccgcgtcc ttctgttcga cctgcttctt gaagcgcgcg gcctggtctt cgggatcgtt cagctccgag aacccgttcg cgatttcgcg gccggtgatg aagagctcga aacgctcggt gatgccggcc accgtgtccg attcgcgcgc gagcggcgac acttcgatcg ggtagtcgat gatgaaggtc ggctcccaca gttgcgcttc cgcggtttcc tcgaagaggg cgagctgcag cgcgccgatg ccggcgttca ggaacgcggg ctgcgtgacg tcgacgccga ggcgcttcag ttcgctgcgc aggaacgcgt cgtccgacag ttggccatcc gtgtagctgg gcgcgtactt ctggatcgcc tgcgtgatcg tcaggcgatg aaacggttgc gcgagatcga gctcgcggcc ctggtactgg atcgtcgcgg tgccgagcgc gtccacggcc gcctggcgga tcagccgctc ggtgaagtcc atcagccagc ggtagtcggt gtacgcggcg tagaactcca tcatcgtgaa ttccggattg tggcgcggcg acacgccctc gttgcggaaa ttccggttga tctcgaacac gcgctcgaag ccgccgacga tgaggcgctt caggtacagc tcgggcgcga tgcgcaggaa catttccatg tcgagcgcgt tgtgatgcgt gacgaacggc ttcgcggccg cgccgcccgg gatcgggtgc agcatcggcg tctcgacttc catgaaatng gcgtcgccca tgaacttgcg gatcgacgcg atcgccttcg tgcgcgcgcg aaacgtcgtg cgcgtctcgg gcgtgacgat caggtcgacg tagcgctggc gatagcgcgt ttcctggtca gcgaggccgt ggaacttgtc gggcagcggg cgcagcgcct tcgcgagcag ccgcagctgc gtgcacttca ccgacagctc gcccttgttc gtgcgaaaca gcacgccgcg cgcggcgacg atgtcgccca ggtcccactt cttgaacgca tcgtacgttt ccgcgccgac gtcggcgggc gtcacgaaga actggatctg acccgagccg tcctgcaccg tcgcgaagct cgccttgccc atcacgcgct tgagcatcat ccggccggcg atcgcgacct cgagcgactt cgcctcgagc gcttccttgt cggcgtccgc gtatgcggtc tgcagatcgg ccgcgtggtg cgtcggctgg aagtcgttcg gataggcgat gccttggtcg cgcagcgcgc gcagcttgtc gcggcgttcc gcgacgatct ggttttcgtc cgctgcgaca gcggcctgcg gttggatcgg ttcggtcatc gaaccaggac cctgggtctg agcttccagg gt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTEPIQPQA AVAADENQIV AERRDKLRAL RDQGIAYPND FQPTHHAADL QTAYADADKE ALEAKSLEVA IAGRMMLKRV MGKASFATVQ DGSGQIQFFV TPADVGAETY DAFKKWDLGD IVAARGVLFR TNKGELSVKC TQLRLLAKAL RPLPDKFHGL ADQETRYRQR YVDLIVTPET RTTFRARTKA IASIRKFMGD ADFMEVETPM LHPIPGGAAA KPFVTHHNAL DMEMFLRIAP ELYLKRLIVG GFERVFEINR NFRNEGVSPR HNPEFTMMEF YAAYTDYRWL MDFTERLIRQ AAVDALGTAT IQYQGRELDL AQPFHRLTIT QAIQKYAPSY TDGQLSDDAF LRSELKRLGV DVTQPAFLNA GIGALQLALF EETAEAQLWE PTFIIDYPIE VSPLARESDT VAGITERFEL FITGREIANG FSELNDPEDQ AARFKKQVEQ KDAGDEEAMF FDADYIRALE YGMPPTGGCG IGIDRLVMLL TDSPTIRDVL LFPHLRRED
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgaaccg atccaaccgc aggccgctgt cgcagcggac gaaaaccaga tcgtcgcgga acgccgcgac aagctgcgcg cgctgcgcga ccaaggcatc gcctatccga acgacttcca gccgacgcac cacgcggccg atctgcagac cgcatacgcg gacgccgaca aggaagcgct cgaggcgaag tcgctcgagg tcgcgatcgc cggccggatg atgctcaagc gcgtgatggg caaggcgagc ttcgcgacgg tgcaggacgg ctcgggtcag atccagttct tcgtgacgcc cgccgacgtc ggcgcggaaa cgtacgatgc gttcaagaag tgggacctgg gcgacatcgt cgccgcgcgc ggcgtgctgt ttcgcacgaa caagggcgag ctgtcggtga agtgcacgca gctgcggctg ctcgcgaagg cgctgcgccc gctgcccgac aagttccacg gcctcgctga ccaggaaacg cgctatcgcc agcgctacgt cgacctgatc gtcacgcccg agacgcgcac gacgtttcgc gcgcgcacga aggcgatcgc gtcgatccgc aagttcatgg gcgacgccga tttcatggaa gtcgagacgc cgatgctgca cccgatcccg ggcggcgcgg ccgcgaagcc gttcgtcacg catcacaacg cgctcgacat ggaaatgttc ctgcgcatcg cgcccgagct gtacctgaag cgcctcatcg tcggcggctt cgagcgcgtg ttcgagatca accggaattt ccgcaacgag ggcgtgtcgc cgcgccacaa tccggaattc acgatgatgg agttctacgc cgcgtacacc gactaccgct ggctgatgga cttcaccgag cggctgatcc gccaggcggc cgtggacgcg ctcggcaccg cgacgatcca gtaccagggc cgcgagctcg atctcgcgca accgtttcat cgcctgacga tcacgcaggc gatccagaag tacgcgccca gctacacgga tggccaactg tcggacgacg cgttcctgcg cagcgaactg aagcgcctcg gcgtcgacgt cacgcagccc gcgttcctga acgccggcat cggcgcgctg cagctcgccc tcttcgagga aaccgcggaa gcgcaactgt gggagccgac cttcatcatc gactacccga tcgaagtgtc gccgctcgcg cgcgaatcgg acacggtggc cggcatcacc gagcgtttcg agctcttcat caccggccgc gaaatcgcga acgggttctc ggagctgaac gatcccgaag accaggccgc gcgcttcaag aagcaggtcg aacagaagga cgcgggcgac gaggaagcga tgttcttcga tgcggactac atccgcgcgc tcgaatacgg gatgcctccg acaggcggct gcgggatcgg catcgaccgt ctcgtgatgc tgctgaccga cagcccgacg atccgcgacg tgctgctgtt cccgcacctg cgtcgcgagg actaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa