ButhA.00507.a

Ketol-acid reductoisomerase (EC 1.1.1.86) (Acetohydroxy-acid isomeroreductase) (Alpha-keto-beta-hydroxylacil reductoisomerase) (BTH_I1047) (ilvC)

CENTER ID: ButhA.00507.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00507.a.A1.GE32743 full length 1 338
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00507.a.A1.PW33347 full length 1 338

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.3807
RefSeq: YP_441601.1
UniProt: Q2SZP8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKVFYDKDAD LSLIKGKQVT IIGYGSQGHA HALNLKDSGV NVTVGLRRGG ASWSKAENAG LAVKEVAEAV KGADVVMMLL PDEQIAAVYA QEVHANIKEG ATLAFAHGFN VHYGQVIPRA DLDVIMVAPK APGHTVRGTY AQGGGVPHLI AVAQDKSGAA RDIALSYAAA NGGGRAGIIE TNFREETETD LFGEQAVLCG GTVELIKAGF ETLVEAGYAP EMAYFECLHE LKLIVDLIYE GGIANMNYSI SNNAEYGEYV TGPRVVTEET KKAMKQCLTD IQTGEYAKSF ILENKAGAPT LQSRRRLTAE HQIEQVGSKL RAMMPWIAKN KLVDQSKN
NT Sequence
atgaaagttt tctacgacaa ggacgccgat ctctccctca tcaagggcaa gcaggtgacg atcatcggtt acggctcgca gggccatgca catgcgctga acctgaagga cagcggcgtg aacgtgacgg tcggcctgcg tcgcggcggc gcgtcgtgga gcaaggccga gaacgccggc cttgcggtca aggaagtcgc cgaagcggtg aagggcgcgg acgtcgtgat gatgctgctg cccgacgagc agatcgccgc ggtctacgcg caggaagtgc acgcgaacat caaggaaggc gcgacgctcg cgttcgcgca cggcttcaac gtccactacg gccaggtgat cccgcgcgcg gatctcgacg tgatcatggt cgcgccgaag gcgccgggcc acacggtgcg cggcacgtac gcgcagggcg gcggcgtgcc gcacctgatc gcggtcgcgc aggacaagtc gggcgcggcg cgcgacatcg cgctgtcgta cgcggcggcg aacggcggcg gccgtgccgg catcatcgag acgaacttcc gcgaagagac ggaaaccgac ctgttcggcg agcaggccgt gctgtgcggc ggcaccgtcg agctgatcaa ggccggtttc gagacgctcg tcgaagcggg ctacgcgccg gaaatggcgt acttcgagtg cctgcacgag ctcaagctga tcgtcgacct gatctatgaa ggcggcatcg cgaacatgaa ctactcgatc tcgaacaacg ccgaatacgg cgagtacgtg acgggcccgc gcgtcgtcac cgaagagacg aagaaggcga tgaagcagtg cctgaccgac atccagacgg gcgagtacgc gaagagcttc atcctcgaga acaaggcggg cgcgccgacg ctgcagtcgc gccgccgcct gacggccgag caccagatcg agcaggtcgg ctcgaaactg cgcgcgatga tgccgtggat cgcgaagaac aagctcgtcg accagtcgaa gaactaa
Details for ButhA.00507.a.A1.GE32743
HARVESTED ON: 6/20/2011
SEQUENCED ON: 5/3/2016
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMKVFYDK DADLSLIKGK QVTIIGYGSQ GHAHALNLKD SGVNVTVGLR RGGASWSKAE NAGLAVKEVA EAVKGADVVM MLLPDEQIAA VYAQEVHANI KEGATLAFAH GFNVHYGQVI PRADLDVIMV APKAPGHTVR GTYAQGGGVP HLIAVAQDKS GAARDIALSY AAANGGGRAG IIETNFREET ETDLFGEQAV LCGGTVELIK AGFETLVEAG YAPEMAYFEC LHELKLIVDL IYEGGIANMN YSISNNAEYG EYVTGPRVVT EETKKAMKQC LTDIQTGEYA KSFILENKAG APTLQSRRRL TAEHQIEQVG SKLRAMMPWI AKNKLVDQSK N
Validated NT Sequence
ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgaaagtt ttctacgaca aggacgccga tctctccctc atcaagggca agcaggtgac gatcatcggt tacggctcgc agggccatgc acatgcgctg aacctgaagg acagcggcgt gaacgtgacg gtcggcctgc gtcgcggcgg cgcgtcgtgg agcaaggccg agaacgccgg ccttgcggtc aaggaagtcg ccgaagcggt gaagggcgcg gacgtcgtga tgatgctgct gcccgacgag cagatcgccg cggtctacgc gcaggaagtg cacgcgaaca tcaaggaagg cgcgacgctc gcgttcgcgc acggcttcaa cgtccactac ggccaggtga tcccgcgcgc ggatctcgac gtgatcatgg tcgcgccgaa ggcgccgggc cacacggtgc gcggcacgta cgcgcagggc ggcggcgtgc cgcacctgat cgcggtcgcg caggacaagt cgggcgcggc gcgcgacatc gcgctgtcgt acgcggcggc gaacggcggc ggccgtgccg gcatcatcga gacgaacttc cgcgaagaga cggaaaccga cctgttcggc gagcaggccg tgctgtgcgg cggcaccgtc gagctgatca aggccggttt cgagacgctc gtcgaagcgg gctacgcgcc ggaaatggcg tacttcgagt gcctgcacga gctcaagctg atcgtcgacc tgatctatga aggcggcatc gcgaacatga actactcgat ctcgaacaac gccgaatacg gcgagtacgt gacgggcccg cgcgtcgtca ccgaagagac gaagaaggcg atgaagcagt gcctgaccga catccagacg ggcgagtacg cgaagagctt catcctcgag aacaaggcgg gcgcgccgac gctgcagtcg cgccgccgcc tgacggccga gcaccagatc gagcaggtcg gctcgaaact gcgcgcgatg atgccgtgga tcgcgaagaa caagctcgtc gaccagtcga agaactaata a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKVFYDKDA DLSLIKGKQV TIIGYGSQGH AHALNLKDSG VNVTVGLRRG GASWSKAENA GLAVKEVAEA VKGADVVMML LPDEQIAAVY AQEVHANIKE GATLAFAHGF NVHYGQVIPR ADLDVIMVAP KAPGHTVRGT YAQGGGVPHL IAVAQDKSGA ARDIALSYAA ANGGGRAGII ETNFREETET DLFGEQAVLC GGTVELIKAG FETLVEAGYA PEMAYFECLH ELKLIVDLIY EGGIANMNYS ISNNAEYGEY VTGPRVVTEE TKKAMKQCLT DIQTGEYAKS FILENKAGAP TLQSRRRLTA EHQIEQVGSK LRAMMPWIAK NKLVDQSKN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaagttttc tacgacaagg acgccgatct ctccctcatc aagggcaagc aggtgacgat catcggttac ggctcgcagg gccatgcaca tgcgctgaac ctgaaggaca gcggcgtgaa cgtgacggtc ggcctgcgtc gcggcggcgc gtcgtggagc aaggccgaga acgccggcct tgcggtcaag gaagtcgccg aagcggtgaa gggcgcggac gtcgtgatga tgctgctgcc cgacgagcag atcgccgcgg tctacgcgca ggaagtgcac gcgaacatca aggaaggcgc gacgctcgcg ttcgcgcacg gcttcaacgt ccactacggc caggtgatcc cgcgcgcgga tctcgacgtg atcatggtcg cgccgaaggc gccgggccac acggtgcgcg gcacgtacgc gcagggcggc ggcgtgccgc acctgatcgc ggtcgcgcag gacaagtcgg gcgcggcgcg cgacatcgcg ctgtcgtacg cggcggcgaa cggcggcggc cgtgccggca tcatcgagac gaacttccgc gaagagacgg aaaccgacct gttcggcgag caggccgtgc tgtgcggcgg caccgtcgag ctgatcaagg ccggtttcga gacgctcgtc gaagcgggct acgcgccgga aatggcgtac ttcgagtgcc tgcacgagct caagctgatc gtcgacctga tctatgaagg cggcatcgcg aacatgaact actcgatctc gaacaacgcc gaatacggcg agtacgtgac gggcccgcgc gtcgtcaccg aagagacgaa gaaggcgatg aagcagtgcc tgaccgacat ccagacgggc gagtacgcga agagcttcat cctcgagaac aaggcgggcg cgccgacgct gcagtcgcgc cgccgcctga cggccgagca ccagatcgag caggtcggct cgaaactgcg cgcgatgatg ccgtggatcg cgaagaacaa gctcgtcgac cagtcgaaga actaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for ButhA.00507.a.A1.PW33347
PURIFICATION DATe: 9/23/2011
CONCENTRATION: 25.62mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMKVFYDK DADLSLIKGK QVTIIGYGSQ GHAHALNLKD SGVNVTVGLR RGGASWSKAE NAGLAVKEVA EAVKGADVVM MLLPDEQIAA VYAQEVHANI KEGATLAFAH GFNVHYGQVI PRADLDVIMV APKAPGHTVR GTYAQGGGVP HLIAVAQDKS GAARDIALSY AAANGGGRAG IIETNFREET ETDLFGEQAV LCGGTVELIK AGFETLVEAG YAPEMAYFEC LHELKLIVDL IYEGGIANMN YSISNNAEYG EYVTGPRVVT EETKKAMKQC LTDIQTGEYA KSFILENKAG APTLQSRRRL TAEHQIEQVG SKLRAMMPWI AKNKLVDQSK N
Validated NT Sequence
ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgaaagtt ttctacgaca aggacgccga tctctccctc atcaagggca agcaggtgac gatcatcggt tacggctcgc agggccatgc acatgcgctg aacctgaagg acagcggcgt gaacgtgacg gtcggcctgc gtcgcggcgg cgcgtcgtgg agcaaggccg agaacgccgg ccttgcggtc aaggaagtcg ccgaagcggt gaagggcgcg gacgtcgtga tgatgctgct gcccgacgag cagatcgccg cggtctacgc gcaggaagtg cacgcgaaca tcaaggaagg cgcgacgctc gcgttcgcgc acggcttcaa cgtccactac ggccaggtga tcccgcgcgc ggatctcgac gtgatcatgg tcgcgccgaa ggcgccgggc cacacggtgc gcggcacgta cgcgcagggc ggcggcgtgc cgcacctgat cgcggtcgcg caggacaagt cgggcgcggc gcgcgacatc gcgctgtcgt acgcggcggc gaacggcggc ggccgtgccg gcatcatcga gacgaacttc cgcgaagaga cggaaaccga cctgttcggc gagcaggccg tgctgtgcgg cggcaccgtc gagctgatca aggccggttt cgagacgctc gtcgaagcgg gctacgcgcc ggaaatggcg tacttcgagt gcctgcacga gctcaagctg atcgtcgacc tgatctatga aggcggcatc gcgaacatga actactcgat ctcgaacaac gccgaatacg gcgagtacgt gacgggcccg cgcgtcgtca ccgaagagac gaagaaggcg atgaagcagt gcctgaccga catccagacg ggcgagtacg cgaagagctt catcctcgag aacaaggcgg gcgcgccgac gctgcagtcg cgccgccgcc tgacggccga gcaccagatc gagcaggtcg gctcgaaact gcgcgcgatg atgccgtgga tcgcgaagaa caagctcgtc gaccagtcga agaactaata a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKVFYDKDA DLSLIKGKQV TIIGYGSQGH AHALNLKDSG VNVTVGLRRG GASWSKAENA GLAVKEVAEA VKGADVVMML LPDEQIAAVY AQEVHANIKE GATLAFAHGF NVHYGQVIPR ADLDVIMVAP KAPGHTVRGT YAQGGGVPHL IAVAQDKSGA ARDIALSYAA ANGGGRAGII ETNFREETET DLFGEQAVLC GGTVELIKAG FETLVEAGYA PEMAYFECLH ELKLIVDLIY EGGIANMNYS ISNNAEYGEY VTGPRVVTEE TKKAMKQCLT DIQTGEYAKS FILENKAGAP TLQSRRRLTA EHQIEQVGSK LRAMMPWIAK NKLVDQSKN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAAGTTTTC TACGACAAGG ACGCCGATCT CTCCCTCATC AAGGGCAAGC AGGTGACGAT CATCGGTTAC GGCTCGCAGG GCCATGCACA TGCGCTGAAC CTGAAGGACA GCGGCGTGAA CGTGACGGTC GGCCTGCGTC GCGGCGGCGC GTCGTGGAGC AAGGCCGAGA ACGCCGGCCT TGCGGTCAAG GAAGTCGCCG AAGCGGTGAA GGGCGCGGAC GTCGTGATGA TGCTGCTGCC CGACGAGCAG ATCGCCGCGG TCTACGCGCA GGAAGTGCAC GCGAACATCA AGGAAGGCGC GACGCTCGCG TTCGCGCACG GCTTCAACGT CCACTACGGC CAGGTGATCC CGCGCGCGGA TCTCGACGTG ATCATGGTCG CGCCGAAGGC GCCGGGCCAC ACGGTGCGCG GCACGTACGC GCAGGGCGGC GGCGTGCCGC ACCTGATCGC GGTCGCGCAG GACAAGTCGG GCGCGGCGCG CGACATCGCG CTGTCGTACG CGGCGGCGAA CGGCGGCGGC CGTGCCGGCA TCATCGAGAC GAACTTCCGC GAAGAGACGG AAACCGACCT GTTCGGCGAG CAGGCCGTGC TGTGCGGCGG CACCGTCGAG CTGATCAAGG CCGGTTTCGA GACGCTCGTC GAAGCGGGCT ACGCGCCGGA AATGGCGTAC TTCGAGTGCC TGCACGAGCT CAAGCTGATC GTCGACCTGA TCTATGAAGG CGGCATCGCG AACATGAACT ACTCGATCTC GAACAACGCC GAATACGGCG AGTACGTGAC GGGCCCGCGC GTCGTCACCG AAGAGACGAA GAAGGCGATG AAGCAGTGCC TGACCGACAT CCAGACGGGC GAGTACGCGA AGAGCTTCAT CCTCGAGAAC AAGGCGGGCG CGCCGACGCT GCAGTCGCGC CGCCGCCTGA CGGCCGAGCA CCAGATCGAG CAGGTCGGCT CGAAACTGCG CGCGATGATG CCGTGGATCG CGAAGAACAA GCTCGTCGAC CAGTCGAAGA ACTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA