ButhA.00483.a
Glutamate 5-kinase (EC 2.7.2.11) (Gamma-glutamyl kinase) (GK) (BTH_I1143) (proB)
| CENTER ID: | ButhA.00483.a |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00483.a.A1.GE32742 | full length | 1 | 372 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00483.a.A1.PW33346 | full length | 1 | 372 |
Structures
4Q1T
DEPOSITED: 4/4/2014
DETERMINATION: XRay
CLONE: ButhA.00483.a.A1.GE32742
PROTEIN: ButhA.00483.a.A1.PW33346
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_129344 |
| PATRIC ID: | fig|271848.6.peg.3898 |
| RefSeq: | YP_441690.1 |
| UniProt: | Q2SZF9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRSIIADSKR LVVKVGSSLV TNDGRGLDHD AIGRWAAQIA ALRNEGKEVV LVSSGAIAEG MQRLGWSRRP REIDELQAAA AVGQMGLAQV YESRFAEHGI RTAQILLTHA DLADRERYLN ARSTLLTLLR LGVVPIINEN DTVVTDEIKF GDNDTLGALV ANLIEGDALI ILTDQQGLFT ADPRKDPGAT LVAEASAGAP ELEAMAGGAG SSIGRGGMLT KILAAKRAAH SGANTVIASG RERDVLLRLA SGEAIGTQLI ARTARMAARK QWMADHLQVR GHVVIDAGAV DKLTAGGKSL LPIGVVAVQG VFARGEVIAC VNDAGREVAR GITNYSSAEA KLIQRKPSGE IEAVLGYMLE PELIHRDNLV LV
NT Sequence
atgcgttcga tcatcgccga ttcgaagcgc ttggtagtga aagtcggctc gagcctcgtc accaacgatg gccgcgggct cgatcatgac gccatcggcc gatgggctgc ccagatcgcc gcgctgcgca acgagggcaa ggaagtggtg cttgtcagct cgggggcgat cgcggaagga atgcagcgcc tgggctggag caggcggccg cgcgagatcg acgagctgca ggctgccgcg gcggtcgggc aaatggggct cgcgcaggtg tatgaaagcc gttttgccga gcacggaatc cgcaccgcgc agattctcct cacgcacgcc gatcttgctg accgcgaacg ctacctgaac gcgcgctcga cgcttctcac gctgctgcgg ctcggcgtcg tgccgatcat caacgagaac gacaccgtcg tcaccgacga aatcaagttc ggcgacaacg acacgctagg cgcgctcgtc gcgaacctga tcgaaggtga cgcgctcatt atcctgaccg atcagcaggg gcttttcacg gccgatccgc gcaaggaccc gggcgcgacg ctcgttgccg aggcaagcgc cggggcgccg gagctcgaag cgatggcggg cggcgcgggc tcgagcatcg gccgcggcgg catgctgacg aagattctcg cggcgaagcg cgctgcgcac agcggcgcga acacggtgat cgcgagcggt cgcgagcgcg acgtgctctt gcggctcgcg tccggcgagg cgatcggcac gcaactgatc gcgcgtaccg ctcggatggc ggcgcgcaag cagtggatgg cggatcacct gcaggtgcgc ggccacgtcg ttatcgatgc gggcgcggtc gacaagctga cggcaggcgg caagagcctg ttgccgatcg gcgtcgtcgc cgtgcagggc gtgttcgcgc gtggcgaagt gattgcgtgc gtcaatgatg caggccgcga agtcgcgcgc gggatcacca attacagcag cgccgaagcg aagctgatcc agcgcaagcc gagcggcgaa atcgaagcgg tgctcggcta catgctcgag cctgaactga tccatcggga caatctcgtg ctcgtctga
Details for ButhA.00483.a.A1.GE32742
| HARVESTED ON: | 6/20/2011 |
| SEQUENCED ON: | 7/14/2011 |
| EXPECTED MW: | 41kDa |
| OBSERVED MW: | 41kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRSIIADSK RLVVKVGSSL VTNDGRGLDH DAIGRWAAQI AALRNEGKEV VLVSSGAIAE GMQRLGWSRR PREIDELQAA AAVGQMGLAQ VYESRFAEHG IRTAQILLTH ADLADRERYL NARSTLLTLL RLGVVPIINE NDTVVTDEIK FGDNDTLGAL VANLIEGDAL IILTDQQGLF TADPRKDPGA TLVAEASAGA PELEAMAGGA GSSIGRGGML TKILAAKRAA HSGANTVIAS GRERDVLLRL ASGEAIGTQL IARTARMAAR KQWMADHLQV RGHVVIDAGA VDKLTAGGKS LLPIGVVAVQ GVFARGEVIA CVNDAGREVA RGITNYSSAE AKLIQRKPSG EIEAVLGYML EPELIHRDNL VLV
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgtt cgatcatcgc cgattcgaag cgcttggtag tgaaagtcgg ctcgagcctc gtcaccaacg atggccgcgg gctcgatcat gacgccatcg gccgatgggc tgcccagatc gccgcgctgc gcaacgaggg caaggaagtg gtgcttgtca gctcgggggc gatcgcggaa ggaatgcagc gcctgggctg gagcaggcgg ccgcgcgaga tcgacgagct gcaggctgcc gcggcggtcg ggcaaatggg gctcgcgcag gtgtatgaaa gccgttttgc cgagcacgga atccgcaccg cgcagattct cctcacgcac gccgatcttg ctgaccgcga acgctacctg aacgcgcgct cgacgcttct cacgctgctg cggctcggcg tcgtgccgat catcaacgag aacgacaccg tcgtcaccga cgaaatcaag ttcggcgaca acgacacgct aggcgcgctc gtcgcgaacc tgatcgaagg tgacgcgctc attatcctga ccgatcagca ggggcttttc acggccgatc cgcgcaagga cccgggcgcg acgctcgttg ccgaggcaag cgccggggcg ccggagctcg aagcgatggc gggcggcgcg ggctcgagca tcggccgcgg cggcatgctg acgaagattc tcgcggcgaa gcgcgctgcg cacagcggcg cgaacacggt gatcgcgagc ggtcgcgagc gcgacgtgct cttgcggctc gcgtccggcg aggcgatcgg cacgcaactg atcgcgcgta ccgctcggat ggcggcgcgc aagcagtgga tggcggatca cctgcaggtg cgcggccacg tcgttatcga tgcgggcgcg gtcgacaagc tgacggcagg cggcaagagc ctgttgccga tcggcgtcgt cgccgtgcag ggcgtgttcg cgcgtggcga agtgattgcg tgcgtcaatg atgcaggccg cgaagtcgcg cgcgggatca ccaattacag cagcgccgaa gcgaagctga tccagcgcaa gccgagcggc gaaatcgaag cggtgctcgg ctacatgctc gagcctgaac tgatccatcg ggacaatctc gtgctcgtct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRSIIADSK RLVVKVGSSL VTNDGRGLDH DAIGRWAAQI AALRNEGKEV VLVSSGAIAE GMQRLGWSRR PREIDELQAA AAVGQMGLAQ VYESRFAEHG IRTAQILLTH ADLADRERYL NARSTLLTLL RLGVVPIINE NDTVVTDEIK FGDNDTLGAL VANLIEGDAL IILTDQQGLF TADPRKDPGA TLVAEASAGA PELEAMAGGA GSSIGRGGML TKILAAKRAA HSGANTVIAS GRERDVLLRL ASGEAIGTQL IARTARMAAR KQWMADHLQV RGHVVIDAGA VDKLTAGGKS LLPIGVVAVQ GVFARGEVIA CVNDAGREVA RGITNYSSAE AKLIQRKPSG EIEAVLGYML EPELIHRDNL VLV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgttcgatc atcgccgatt cgaagcgctt ggtagtgaaa gtcggctcga gcctcgtcac caacgatggc cgcgggctcg atcatgacgc catcggccga tgggctgccc agatcgccgc gctgcgcaac gagggcaagg aagtggtgct tgtcagctcg ggggcgatcg cggaaggaat gcagcgcctg ggctggagca ggcggccgcg cgagatcgac gagctgcagg ctgccgcggc ggtcgggcaa atggggctcg cgcaggtgta tgaaagccgt tttgccgagc acggaatccg caccgcgcag attctcctca cgcacgccga tcttgctgac cgcgaacgct acctgaacgc gcgctcgacg cttctcacgc tgctgcggct cggcgtcgtg ccgatcatca acgagaacga caccgtcgtc accgacgaaa tcaagttcgg cgacaacgac acgctaggcg cgctcgtcgc gaacctgatc gaaggtgacg cgctcattat cctgaccgat cagcaggggc ttttcacggc cgatccgcgc aaggacccgg gcgcgacgct cgttgccgag gcaagcgccg gggcgccgga gctcgaagcg atggcgggcg gcgcgggctc gagcatcggc cgcggcggca tgctgacgaa gattctcgcg gcgaagcgcg ctgcgcacag cggcgcgaac acggtgatcg cgagcggtcg cgagcgcgac gtgctcttgc ggctcgcgtc cggcgaggcg atcggcacgc aactgatcgc gcgtaccgct cggatggcgg cgcgcaagca gtggatggcg gatcacctgc aggtgcgcgg ccacgtcgtt atcgatgcgg gcgcggtcga caagctgacg gcaggcggca agagcctgtt gccgatcggc gtcgtcgccg tgcagggcgt gttcgcgcgt ggcgaagtga ttgcgtgcgt caatgatgca ggccgcgaag tcgcgcgcgg gatcaccaat tacagcagcg ccgaagcgaa gctgatccag cgcaagccga gcggcgaaat cgaagcggtg ctcggctaca tgctcgagcc tgaactgatc catcgggaca atctcgtgct cgtctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for ButhA.00483.a.A1.PW33346
| PURIFICATION DATe: | 8/13/2011 |
| CONCENTRATION: | 36.96mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRSIIADSK RLVVKVGSSL VTNDGRGLDH DAIGRWAAQI AALRNEGKEV VLVSSGAIAE GMQRLGWSRR PREIDELQAA AAVGQMGLAQ VYESRFAEHG IRTAQILLTH ADLADRERYL NARSTLLTLL RLGVVPIINE NDTVVTDEIK FGDNDTLGAL VANLIEGDAL IILTDQQGLF TADPRKDPGA TLVAEASAGA PELEAMAGGA GSSIGRGGML TKILAAKRAA HSGANTVIAS GRERDVLLRL ASGEAIGTQL IARTARMAAR KQWMADHLQV RGHVVIDAGA VDKLTAGGKS LLPIGVVAVQ GVFARGEVIA CVNDAGREVA RGITNYSSAE AKLIQRKPSG EIEAVLGYML EPELIHRDNL VLV
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgtt cgatcatcgc cgattcgaag cgcttggtag tgaaagtcgg ctcgagcctc gtcaccaacg atggccgcgg gctcgatcat gacgccatcg gccgatgggc tgcccagatc gccgcgctgc gcaacgaggg caaggaagtg gtgcttgtca gctcgggggc gatcgcggaa ggaatgcagc gcctgggctg gagcaggcgg ccgcgcgaga tcgacgagct gcaggctgcc gcggcggtcg ggcaaatggg gctcgcgcag gtgtatgaaa gccgttttgc cgagcacgga atccgcaccg cgcagattct cctcacgcac gccgatcttg ctgaccgcga acgctacctg aacgcgcgct cgacgcttct cacgctgctg cggctcggcg tcgtgccgat catcaacgag aacgacaccg tcgtcaccga cgaaatcaag ttcggcgaca acgacacgct aggcgcgctc gtcgcgaacc tgatcgaagg tgacgcgctc attatcctga ccgatcagca ggggcttttc acggccgatc cgcgcaagga cccgggcgcg acgctcgttg ccgaggcaag cgccggggcg ccggagctcg aagcgatggc gggcggcgcg ggctcgagca tcggccgcgg cggcatgctg acgaagattc tcgcggcgaa gcgcgctgcg cacagcggcg cgaacacggt gatcgcgagc ggtcgcgagc gcgacgtgct cttgcggctc gcgtccggcg aggcgatcgg cacgcaactg atcgcgcgta ccgctcggat ggcggcgcgc aagcagtgga tggcggatca cctgcaggtg cgcggccacg tcgttatcga tgcgggcgcg gtcgacaagc tgacggcagg cggcaagagc ctgttgccga tcggcgtcgt cgccgtgcag ggcgtgttcg cgcgtggcga agtgattgcg tgcgtcaatg atgcaggccg cgaagtcgcg cgcgggatca ccaattacag cagcgccgaa gcgaagctga tccagcgcaa gccgagcggc gaaatcgaag cggtgctcgg ctacatgctc gagcctgaac tgatccatcg ggacaatctc gtgctcgtct
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRSIIADSK RLVVKVGSSL VTNDGRGLDH DAIGRWAAQI AALRNEGKEV VLVSSGAIAE GMQRLGWSRR PREIDELQAA AAVGQMGLAQ VYESRFAEHG IRTAQILLTH ADLADRERYL NARSTLLTLL RLGVVPIINE NDTVVTDEIK FGDNDTLGAL VANLIEGDAL IILTDQQGLF TADPRKDPGA TLVAEASAGA PELEAMAGGA GSSIGRGGML TKILAAKRAA HSGANTVIAS GRERDVLLRL ASGEAIGTQL IARTARMAAR KQWMADHLQV RGHVVIDAGA VDKLTAGGKS LLPIGVVAVQ GVFARGEVIA CVNDAGREVA RGITNYSSAE AKLIQRKPSG EIEAVLGYML EPELIHRDNL VLV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGTTCGATC ATCGCCGATT CGAAGCGCTT GGTAGTGAAA GTCGGCTCGA GCCTCGTCAC CAACGATGGC CGCGGGCTCG ATCATGACGC CATCGGCCGA TGGGCTGCCC AGATCGCCGC GCTGCGCAAC GAGGGCAAGG AAGTGGTGCT TGTCAGCTCG GGGGCGATCG CGGAAGGAAT GCAGCGCCTG GGCTGGAGCA GGCGGCCGCG CGAGATCGAC GAGCTGCAGG CTGCCGCGGC GGTCGGGCAA ATGGGGCTCG CGCAGGTGTA TGAAAGCCGT TTTGCCGAGC ACGGAATCCG CACCGCGCAG ATTCTCCTCA CGCACGCCGA TCTTGCTGAC CGCGAACGCT ACCTGAACGC GCGCTCGACG CTTCTCACGC TGCTGCGGCT CGGCGTCGTG CCGATCATCA ACGAGAACGA CACCGTCGTC ACCGACGAAA TCAAGTTCGG CGACAACGAC ACGCTAGGCG CGCTCGTCGC GAACCTGATC GAAGGTGACG CGCTCATTAT CCTGACCGAT CAGCAGGGGC TTTTCACGGC CGATCCGCGC AAGGACCCGG GCGCGACGCT CGTTGCCGAG GCAAGCGCCG GGGCGCCGGA GCTCGAAGCG ATGGCGGGCG GCGCGGGCTC GAGCATCGGC CGCGGCGGCA TGCTGACGAA GATTCTCGCG GCGAAGCGCG CTGCGCACAG CGGCGCGAAC ACGGTGATCG CGAGCGGTCG CGAGCGCGAC GTGCTCTTGC GGCTCGCGTC CGGCGAGGCG ATCGGCACGC AACTGATCGC GCGTACCGCT CGGATGGCGG CGCGCAAGCA GTGGATGGCG GATCACCTGC AGGTGCGCGG CCACGTCGTT ATCGATGCGG GCGCGGTCGA CAAGCTGACG GCAGGCGGCA AGAGCCTGTT GCCGATCGGC GTCGTCGCCG TGCAGGGCGT GTTCGCGCGT GGCGAAGTGA TTGCGTGCGT CAATGATGCA GGCCGCGAAG TCGCGCGCGG GATCACCAAT TACAGCAGCG CCGAAGCGAA GCTGATCCAG CGCAAGCCGA GCGGCGAAAT CGAAGCGGTG CTCGGCTACA TGCTCGAGCC TGAACTGATC CATCGGGACA ATCTCGTGCT CGTCTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA