ButhA.00448.a
Nicotinate (Nicotinamide) nucleotide adenylyltransferase (EC 2.7.7.18) (BTH_I1012) (nadD)
| CENTER ID: | ButhA.00448.a |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00448.a.A1.GE32740 | full length | 1 | 250 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00448.a.A1.PS01231 | full length | 1 | 250 |
Structures
4WSO
DEPOSITED: 10/28/2014
DETERMINATION: XRay
CLONE: ButhA.00448.a.A1.GE32740
PROTEIN: ButhA.00448.a.A1.PS01231
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_131883 |
| PATRIC ID: | fig|271848.6.peg.3761 |
| RefSeq: | YP_441566.1 |
| UniProt: | Q2SZT3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRSATGRVA APQGFAPNAE PAGLPALPRR IGILGGTFDP IHDGHLALAR RFADVLRLTE LVLMPAGQPY QKQDVSAAEH RLAMTRAAAG SLVLPGVAVS VATDEIEHAG PTYTVETLER WRERLGADAS LSLLIGADQL VRLDTWRDWR RLFDFAHVCA ATRPGFDFAA ASPAVAAEIA SRQASADVLR ATPAGRLLID TTLALDVAAT DIRAHLRACI ARHAQVPDAS AEHVSPAVWA YILQHRLYHP
NT Sequence
atgagtcgct ccgccaccgg ccgagtggcc gctccgcaag gctttgcgcc aaacgccgag cccgccggcc tgcccgcgct gccgcgccgc atcggcatcc tcggcggcac gttcgacccg attcacgacg gccatctcgc gctcgcgcgc cgcttcgccg acgtgctgcg cctgaccgag ctcgtgctga tgccggccgg ccagccctat cagaagcagg acgtgtcggc ggccgagcac cggctcgcga tgacccgcgc ggcggcgggc tcgctcgtgc tgccgggcgt ggccgtcagc gtcgcgaccg acgagatcga gcacgccggg ccgacctaca ccgtcgagac gctcgaacgc tggcgcgagc gtctcggcgc cgacgcgtcg ctgtcgctgc tgatcggcgc ggatcaactc gtgcgcctcg acacgtggcg cgactggcgc cgcctcttcg acttcgcgca cgtatgcgcg gcgacgcggc cgggcttcga cttcgcggcg gcgtcgcccg ccgtcgcggc ggagatcgcg agccggcagg cgagcgcgga cgtgctgcgg gcgacgcccg ccggacgtct tctgatcgac acgacgctcg cgctcgacgt cgccgcgacc gacatccgcg cgcacctgcg cgcctgcatc gcgcgccacg cgcaggtacc cgacgcatcg gccgagcacg tgagccccgc cgtctgggcc tatattctcc aacaccgtct ctaccatccc tga
Details for ButhA.00448.a.A1.GE32740
| HARVESTED ON: | 6/20/2011 |
| SEQUENCED ON: | 7/14/2011 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSRSATGRV AAPQGFAPNA EPAGLPALPR RIGILGGTFD PIHDGHLALA RRFADVLRLT ELVLMPAGQP YQKQDVSAAE HRLAMTRAAA GSLVLPGVAV SVATDEIEHA GPTYTVETLE RWRERLGADA SLSLLIGADQ LVRLDTWRDW RRLFDFAHVC AATRPGFDFA AASPAVAAEI ASRQASADVL RATPAGRLLI DTTLALDVAA TDIRAHLRAC IARHAQVPDA SAEHVSPAVW AYILQHRLYH P
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagtc gctccgccac cggccgagtg gccgctccgc aaggctttgc gccaaacgcc gagcccgccg gcctgcccgc gctgccgcgc cgcatcggca tcctcggcgg cacgttcgac ccgattcacg acggccatct cgcgctcgcg cgccgcttcg ccgacgtgct gcgcctgacc gagctcgtgc tgatgccggc cggccagccc tatcagaagc aggacgtgtc ggcggccgag caccggctcg cgatgacccg cgcggcggcg ggctcgctcg tgctgccggg cgtggccgtc agcgtcgcga ccgacgagat cgagcacgcc gggccgacct acaccgtcga gacgctcgaa cgctggcgcg agcgtctcgg cgccgacgcg tcgctgtcgc tgctgatcgg cgcggatcaa ctcgtgcgcc tcgacacgtg gcgcgactgg cgccgcctct tcgacttcgc gcacgtatgc gcggcgacgc ggccgggctt cgacttcgcg gcggcgtcgc ccgccgtcgc ggcggagatc gcgagccggc aggcgagcgc ggacgtgctg cgggcgacgc ccgccggacg tcttctgatc gacacgacgc tcgcgctcga cgtcgccgcg accgacatcc gcgcgcacct gcgcgcctgc atcgcgcgcc acgcgcaggt acccgacgca tcggccgagc acgtgagccc cgccgtctgg gcctatattc tccaacaccg tctctaccat ccctaata
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSRSATGRV AAPQGFAPNA EPAGLPALPR RIGILGGTFD PIHDGHLALA RRFADVLRLT ELVLMPAGQP YQKQDVSAAE HRLAMTRAAA GSLVLPGVAV SVATDEIEHA GPTYTVETLE RWRERLGADA SLSLLIGADQ LVRLDTWRDW RRLFDFAHVC AATRPGFDFA AASPAVAAEI ASRQASADVL RATPAGRLLI DTTLALDVAA TDIRAHLRAC IARHAQVPDA SAEHVSPAVW AYILQHRLYH P
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtcgctcc gccaccggcc gagtggccgc tccgcaaggc tttgcgccaa acgccgagcc cgccggcctg cccgcgctgc cgcgccgcat cggcatcctc ggcggcacgt tcgacccgat tcacgacggc catctcgcgc tcgcgcgccg cttcgccgac gtgctgcgcc tgaccgagct cgtgctgatg ccggccggcc agccctatca gaagcaggac gtgtcggcgg ccgagcaccg gctcgcgatg acccgcgcgg cggcgggctc gctcgtgctg ccgggcgtgg ccgtcagcgt cgcgaccgac gagatcgagc acgccgggcc gacctacacc gtcgagacgc tcgaacgctg gcgcgagcgt ctcggcgccg acgcgtcgct gtcgctgctg atcggcgcgg atcaactcgt gcgcctcgac acgtggcgcg actggcgccg cctcttcgac ttcgcgcacg tatgcgcggc gacgcggccg ggcttcgact tcgcggcggc gtcgcccgcc gtcgcggcgg agatcgcgag ccggcaggcg agcgcggacg tgctgcgggc gacgcccgcc ggacgtcttc tgatcgacac gacgctcgcg ctcgacgtcg ccgcgaccga catccgcgcg cacctgcgcg cctgcatcgc gcgccacgcg caggtacccg acgcatcggc cgagcacgtg agccccgccg tctgggccta tattctccaa caccgtctct accatcccta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for ButhA.00448.a.A1.PS01231
| PURIFICATION DATe: | 10/4/2011 |
| CONCENTRATION: | 41.6mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSRSATGRV AAPQGFAPNA EPAGLPALPR RIGILGGTFD PIHDGHLALA RRFADVLRLT ELVLMPAGQP YQKQDVSAAE HRLAMTRAAA GSLVLPGVAV SVATDEIEHA GPTYTVETLE RWRERLGADA SLSLLIGADQ LVRLDTWRDW RRLFDFAHVC AATRPGFDFA AASPAVAAEI ASRQASADVL RATPAGRLLI DTTLALDVAA TDIRAHLRAC IARHAQVPDA SAEHVSPAVW AYILQHRLYH P
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagtc gctccgccac cggccgagtg gccgctccgc aaggctttgc gccaaacgcc gagcccgccg gcctgcccgc gctgccgcgc cgcatcggca tcctcggcgg cacgttcgac ccgattcacg acggccatct cgcgctcgcg cgccgcttcg ccgacgtgct gcgcctgacc gagctcgtgc tgatgccggc cggccagccc tatcagaagc aggacgtgtc ggcggccgag caccggctcg cgatgacccg cgcggcggcg ggctcgctcg tgctgccggg cgtggccgtc agcgtcgcga ccgacgagat cgagcacgcc gggccgacct acaccgtcga gacgctcgaa cgctggcgcg agcgtctcgg cgccgacgcg tcgctgtcgc tgctgatcgg cgcggatcaa ctcgtgcgcc tcgacacgtg gcgcgactgg cgccgcctct tcgacttcgc gcacgtatgc gcggcgacgc ggccgggctt cgacttcgcg gcggcgtcgc ccgccgtcgc ggcggagatc gcgagccggc aggcgagcgc ggacgtgctg cgggcgacgc ccgccggacg tcttctgatc gacacgacgc tcgcgctcga cgtcgccgcg accgacatcc gcgcgcacct gcgcgcctgc atcgcgcgcc acgcgcaggt acccgacgca tcggccgagc acgtgagccc cgccgtctgg gcctatattc tccaacaccg tctctaccat ccctaata
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSRSATGRV AAPQGFAPNA EPAGLPALPR RIGILGGTFD PIHDGHLALA RRFADVLRLT ELVLMPAGQP YQKQDVSAAE HRLAMTRAAA GSLVLPGVAV SVATDEIEHA GPTYTVETLE RWRERLGADA SLSLLIGADQ LVRLDTWRDW RRLFDFAHVC AATRPGFDFA AASPAVAAEI ASRQASADVL RATPAGRLLI DTTLALDVAA TDIRAHLRAC IARHAQVPDA SAEHVSPAVW AYILQHRLYH P
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGTCGCTCC GCCACCGGCC GAGTGGCCGC TCCGCAAGGC TTTGCGCCAA ACGCCGAGCC CGCCGGCCTG CCCGCGCTGC CGCGCCGCAT CGGCATCCTC GGCGGCACGT TCGACCCGAT TCACGACGGC CATCTCGCGC TCGCGCGCCG CTTCGCCGAC GTGCTGCGCC TGACCGAGCT CGTGCTGATG CCGGCCGGCC AGCCCTATCA GAAGCAGGAC GTGTCGGCGG CCGAGCACCG GCTCGCGATG ACCCGCGCGG CGGCGGGCTC GCTCGTGCTG CCGGGCGTGG CCGTCAGCGT CGCGACCGAC GAGATCGAGC ACGCCGGGCC GACCTACACC GTCGAGACGC TCGAACGCTG GCGCGAGCGT CTCGGCGCCG ACGCGTCGCT GTCGCTGCTG ATCGGCGCGG ATCAACTCGT GCGCCTCGAC ACGTGGCGCG ACTGGCGCCG CCTCTTCGAC TTCGCGCACG TATGCGCGGC GACGCGGCCG GGCTTCGACT TCGCGGCGGC GTCGCCCGCC GTCGCGGCGG AGATCGCGAG CCGGCAGGCG AGCGCGGACG TGCTGCGGGC GACGCCCGCC GGACGTCTTC TGATCGACAC GACGCTCGCG CTCGACGTCG CCGCGACCGA CATCCGCGCG CACCTGCGCG CCTGCATCGC GCGCCACGCG CAGGTACCCG ACGCATCGGC CGAGCACGTG AGCCCCGCCG TCTGGGCCTA TATTCTCCAA CACCGTCTCT ACCATCCCTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A