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Seattle Structural Genomics Center for Infectious Disease

ButhA.00438.a: Nucleoside diphosphate kinase (NDK) (NDP kinase) (EC 2.7.4.6) (Nucleoside-2-P kinase) (BTH_I2231) (ndk)

Target Characteristics

from organism: Burkholderia thailandensis E264
most recent status: in PDB
center reference id: ButhA.00438.a
is community request: True
associated disease:
NIH risk group: 3
is select agent: False
NIH priority
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
ButhA.00438.a.A1.GE32739 full length 1 141 GE32739
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
ButhA.00438.a.A1.PS01187 full length 1 141

Structures

image of structure for 4DUT
4DUT
Deposited: 2/22/2012
Determination: XRay
Diffraction Image
Clone: ButhA.00438.a.A1.GE32739
Protein: ButhA.00438.a.A1.PS01187
image of structure for 4EK2
4EK2
Deposited: 4/9/2012
Determination: XRay
Diffraction Image
Clone: ButhA.00438.a.A1.GE32739
Protein: ButhA.00438.a.A1.PS01187
image of structure for 4HR2
4HR2
Deposited: 10/26/2012
Determination: XRay
Diffraction Image
Clone: ButhA.00438.a.A1.GE32739
Protein: ButhA.00438.a.A1.PS01187

Publications by SSGCID

Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365

External Resources

Resource Reference ID
OrthoMCL: OG5_126708
PATRIC ID: fig|271848.6.peg.5051
RefSeq: YP_442752.1
UniProt: Q2SWE7

Enzyme & Pathway Information

Pathway Pathway ID EC Number
superpathway of purine nucleotides de novo biosynthesis II DENOVOPURINE2-PWY 2.7.4.6
ppGpp biosynthesis PPGPPMET-PWY 2.7.4.6
superpathway of histidine, purine, and pyrimidine biosynthesis PRPP-PWY 2.7.4.6
superpathway of pyrimidine ribonucleotides de novo biosynthesis PWY0-162 2.7.4.6
salvage pathways of pyrimidine ribonucleotides PWY0-163 2.7.4.6
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) PWY0-166 2.7.4.6
pyrimidine ribonucleotides interconversion PWY-5687 2.7.4.6
superpathway of guanosine nucleotides de novo biosynthesis II PWY-6125 2.7.4.6
superpathway of adenosine nucleotides de novo biosynthesis II PWY-6126 2.7.4.6
pyrimidine deoxyribonucleotides de novo biosynthesis III PWY-6545 2.7.4.6
superpathway of purine nucleotide salvage PWY66-409 2.7.4.6
UTP and CTP de novo biosynthesis PWY-7176 2.7.4.6
pyrimidine deoxyribonucleotides de novo biosynthesis I PWY-7184 2.7.4.6
pyrimidine deoxyribonucleotides de novo biosynthesis II PWY-7187 2.7.4.6
superpathway of pyrimidine ribonucleosides salvage PWY-7196 2.7.4.6
pyrimidine deoxyribonucleotide phosphorylation PWY-7197 2.7.4.6
pyrimidine deoxyribonucleotides de novo biosynthesis IV PWY-7198 2.7.4.6
superpathway of pyrimidine deoxyribonucleoside salvage PWY-7200 2.7.4.6
CMP phosphorylation PWY-7205 2.7.4.6
superpathway of pyrimidine nucleobases salvage PWY-7208 2.7.4.6
pyrimidine deoxyribonucleotides biosynthesis from CTP PWY-7210 2.7.4.6
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis PWY-7211 2.7.4.6
adenosine deoxyribonucleotides de novo biosynthesis II PWY-7220 2.7.4.6
guanosine ribonucleotides de novo biosynthesis PWY-7221 2.7.4.6
guanosine deoxyribonucleotides de novo biosynthesis II PWY-7222 2.7.4.6
purine deoxyribonucleosides salvage PWY-7224 2.7.4.6
guanosine deoxyribonucleotides de novo biosynthesis I PWY-7226 2.7.4.6
adenosine deoxyribonucleotides de novo biosynthesis PWY-7227 2.7.4.6
superpathway of guanosine nucleotides de novo biosynthesis I PWY-7228 2.7.4.6
superpathway of adenosine nucleotides de novo biosynthesis I PWY-7229 2.7.4.6
superpathway of purine nucleotides de novo biosynthesis I PWY-841 2.7.4.6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MALERTLSII KPDAVAKNVI GQIYSRFENA GLKIVAARMA HLSRADAEKF YAVHAERPFF KDLVEFMISG PVMIQVLEGE DAILKNRDLM GATDPKKAEK GTIRADFADS IDANAVHGSD APETARVEIA FFFPEMNVYS R

NT Sequence
atggcactcg agcgcaccct gtcgatcatc aagccggatg cggtggcgaa gaacgtgatc ggccagatct acagccgttt cgaaaacgcc ggcctgaaga tcgtggcggc gcgcatggcg cacctgtcgc gcgccgatgc ggagaagttc tacgccgtgc acgccgagcg tccgttcttc aaggatctcg tcgaattcat gatctcgggc ccggtgatga tccaggttct ggaaggcgag gacgcgatcc tgaagaaccg cgacctgatg ggcgcgacgg atccgaagaa ggcggaaaag ggcacgatcc gcgccgactt cgcggatagc atcgacgcga acgccgtgca cggctcggac gcaccggaaa cggcgcgcgt cgagatcgcg ttcttcttcc cggaaatgaa cgtctactcg cgctaa

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