ButhA.00428.a
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (UDP-N-acetylglucosamine acyltransferase) (EC 2.3.1.129) (BTH_I2039) (lpxA)
| CENTER ID: | ButhA.00428.a |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00428.a.A1.GE32738 | full length | 1 | 262 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00428.a.A1.PS01226 | full length | 1 | 262 |
Structures
4EQY
DEPOSITED: 4/19/2012
DETERMINATION: XRay
CLONE: ButhA.00428.a.A1.GE32738
PROTEIN: ButhA.00428.a.A1.PS01225
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|271848.6.peg.4849 |
| RefSeq: | YP_442563.1 |
| UniProt: | Q2SWY6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRIHPTAII EPGAQLHETV EVGPYAIVGS NVTIGARTTI GSHSVIEGHT TIGEDNRIGH YASVGGRPQD MKYKDEPTRL VIGDRNTIRE FTTIHTGTVQ DAGVTTLGDD NWIMAYVHIG HDCRVGSHVV LSSNAQMAGH VEIGDWAIVG GMSGVHQYVR IGAHSMLGGA SALVQDIPPF VIAAGNKAEP HGINVEGLRR RGFSPDAISA LRSAYRILYK NSLSLEEAKV QLSELAQAGG DGDAAVKALV DFVESSQRGI IR
NT Sequence
atgagcagga ttcaccccac ggcgatcatc gagccgggcg cgcagctcca cgagacggtc gaggtcggcc catacgcgat cgtcggttcg aacgtgacga tcggcgcgcg cacgacgatc ggctcgcaca gcgtgatcga aggccatacg acgatcggcg aagacaatcg catcggccat tacgcgtcgg tgggcggccg tccgcaggac atgaagtaca aggacgagcc gacgcggctc gtgatcggcg atcgcaacac gatccgcgaa ttcacgacga tccacacggg caccgttcag gacgcgggcg tcacgacgct cggcgacgac aactggatca tggcgtacgt gcacatcggt cacgactgcc gcgtcggcag ccacgtggtc ctgtcgagca acgcgcagat ggcggggcac gtcgagatcg gcgactgggc gatcgtcggc gggatgtcgg gcgttcacca gtacgtgcga atcggcgcgc attcgatgct gggcggcgcg tccgcgctcg tgcaggacat tccgccgttc gtgatcgcgg cgggcaacaa ggccgagccg cacgggatca acgtcgaagg gctgcgccga cgcggcttct cgccggacgc aatttccgcg ctgcgcagcg cgtaccgtat cctgtacaag aacagcctgt cgctcgaaga ggcgaaggtc cagctgagcg agctcgcgca ggcgggcggc gacggcgacg cggccgtgaa ggcgctcgtc gacttcgtcg agtcgtcgca gcgcggcatc attcgctga
Details for ButhA.00428.a.A1.GE32738
| HARVESTED ON: | 6/20/2011 |
| SEQUENCED ON: | 7/14/2011 |
| EXPECTED MW: | 30kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSRIHPTAI IEPGAQLHET VEVGPYAIVG SNVTIGARTT IGSHSVIEGH TTIGEDNRIG HYASVGGRPQ DMKYKDEPTR LVIGDRNTIR EFTTIHTGTV QDAGVTTLGD DNWIMAYVHI GHDCRVGSHV VLSSNAQMAG HVEIGDWAIV GGMSGVHQYV RIGAHSMLGG ASALVQDIPP FVIAAGNKAE PHGINVEGLR RRGFSPDAIS ALRSAYRILY KNSLSLEEAK VQLSELAQAG GDGDAAVKAL VDFVESSQRG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagca ggattcaccc cacggcgatc atcgagccgg gcgcgcagct ccacgagacg gtcgaggtcg gcccatacgc gatcgtcggt tcgaacgtga cgatcggcgc gcgcacgacg atcggctcgc acagcgtgat cgaaggccat acgacgatcg gcgaagacaa tcgcatcggc cattacgcgt cggtgggcgg ccgtccgcag gacatgaagt acaaggacga gccgacgcgg ctcgtgatcg gcgatcgcaa cacgatccgc gaattcacga cgatccacac gggcaccgtt caggacgcgg gcgtcacgac gctcggcgac gacaactgga tcatggcgta cgtgcacatc ggtcacgact gccgcgtcgg cagccacgtg gtcctgtcga gcaacgcgca gatggcgggg cacgtcgaga tcggcgactg ggcgatcgtc ggcgggatgt cgggcgttca ccagtacgtg cgaatcggcg cgcattcgat gctgggcggc gcgtccgcgc tcgtgcagga cattccgccg ttcgtgatcg cggcgggcaa caaggccgag ccgcacggga tcaacgtcga agggctgcgc cgacgcggct tctcgccgga cgcaatttcc gcgctgcgca gcgcgtaccg tatcctgtac aagaacagcc tgtcgctcga agaggcgaag gtccagctga gcgagctcgc gcaggcgggc ggcgacggcg acgcggccgt gaaggcgctc gtcgacttcg tcgagtcgtc gcagcgcggc nt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSRIHPTAI IEPGAQLHET VEVGPYAIVG SNVTIGARTT IGSHSVIEGH TTIGEDNRIG HYASVGGRPQ DMKYKDEPTR LVIGDRNTIR EFTTIHTGTV QDAGVTTLGD DNWIMAYVHI GHDCRVGSHV VLSSNAQMAG HVEIGDWAIV GGMSGVHQYV RIGAHSMLGG ASALVQDIPP FVIAAGNKAE PHGINVEGLR RRGFSPDAIS ALRSAYRILY KNSLSLEEAK VQLSELAQAG GDGDAAVKAL VDFVESSQRG IIR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcaggatt caccccacgg cgatcatcga gccgggcgcg cagctccacg agacggtcga ggtcggccca tacgcgatcg tcggttcgaa cgtgacgatc ggcgcgcgca cgacgatcgg ctcgcacagc gtgatcgaag gccatacgac gatcggcgaa gacaatcgca tcggccatta cgcgtcggtg ggcggccgtc cgcaggacat gaagtacaag gacgagccga cgcggctcgt gatcggcgat cgcaacacga tccgcgaatt cacgacgatc cacacgggca ccgttcagga cgcgggcgtc acgacgctcg gcgacgacaa ctggatcatg gcgtacgtgc acatcggtca cgactgccgc gtcggcagcc acgtggtcct gtcgagcaac gcgcagatgg cggggcacgt cgagatcggc gactgggcga tcgtcggcgg gatgtcgggc gttcaccagt acgtgcgaat cggcgcgcat tcgatgctgg gcggcgcgtc cgcgctcgtg caggacattc cgccgttcgt gatcgcggcg ggcaacaagg ccgagccgca cgggatcaac gtcgaagggc tgcgccgacg cggcttctcg ccggacgcaa tttccgcgct gcgcagcgcg taccgtatcc tgtacaagaa cagcctgtcg ctcgaagagg cgaaggtcca gctgagcgag ctcgcgcagg cgggcggcga cggcgacgcg gccgtgaagg cgctcgtcga cttcgtcgag tcgtcgcagc gcggcatcat tcgctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for ButhA.00428.a.A1.PS01226
| PURIFICATION DATe: | 10/6/2011 |
| CONCENTRATION: | 71.74mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSRIHPTAI IEPGAQLHET VEVGPYAIVG SNVTIGARTT IGSHSVIEGH TTIGEDNRIG HYASVGGRPQ DMKYKDEPTR LVIGDRNTIR EFTTIHTGTV QDAGVTTLGD DNWIMAYVHI GHDCRVGSHV VLSSNAQMAG HVEIGDWAIV GGMSGVHQYV RIGAHSMLGG ASALVQDIPP FVIAAGNKAE PHGINVEGLR RRGFSPDAIS ALRSAYRILY KNSLSLEEAK VQLSELAQAG GDGDAAVKAL VDFVESSQRG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagca ggattcaccc cacggcgatc atcgagccgg gcgcgcagct ccacgagacg gtcgaggtcg gcccatacgc gatcgtcggt tcgaacgtga cgatcggcgc gcgcacgacg atcggctcgc acagcgtgat cgaaggccat acgacgatcg gcgaagacaa tcgcatcggc cattacgcgt cggtgggcgg ccgtccgcag gacatgaagt acaaggacga gccgacgcgg ctcgtgatcg gcgatcgcaa cacgatccgc gaattcacga cgatccacac gggcaccgtt caggacgcgg gcgtcacgac gctcggcgac gacaactgga tcatggcgta cgtgcacatc ggtcacgact gccgcgtcgg cagccacgtg gtcctgtcga gcaacgcgca gatggcgggg cacgtcgaga tcggcgactg ggcgatcgtc ggcgggatgt cgggcgttca ccagtacgtg cgaatcggcg cgcattcgat gctgggcggc gcgtccgcgc tcgtgcagga cattccgccg ttcgtgatcg cggcgggcaa caaggccgag ccgcacggga tcaacgtcga agggctgcgc cgacgcggct tctcgccgga cgcaatttcc gcgctgcgca gcgcgtaccg tatcctgtac aagaacagcc tgtcgctcga agaggcgaag gtccagctga gcgagctcgc gcaggcgggc ggcgacggcg acgcggccgt gaaggcgctc gtcgacttcg tcgagtcgtc gcagcgcggc nt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSRIHPTAI IEPGAQLHET VEVGPYAIVG SNVTIGARTT IGSHSVIEGH TTIGEDNRIG HYASVGGRPQ DMKYKDEPTR LVIGDRNTIR EFTTIHTGTV QDAGVTTLGD DNWIMAYVHI GHDCRVGSHV VLSSNAQMAG HVEIGDWAIV GGMSGVHQYV RIGAHSMLGG ASALVQDIPP FVIAAGNKAE PHGINVEGLR RRGFSPDAIS ALRSAYRILY KNSLSLEEAK VQLSELAQAG GDGDAAVKAL VDFVESSQRG IIR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCAGGATT CACCCCACGG CGATCATCGA GCCGGGCGCG CAGCTCCACG AGACGGTCGA GGTCGGCCCA TACGCGATCG TCGGTTCGAA CGTGACGATC GGCGCGCGCA CGACGATCGG CTCGCACAGC GTGATCGAAG GCCATACGAC GATCGGCGAA GACAATCGCA TCGGCCATTA CGCGTCGGTG GGCGGCCGTC CGCAGGACAT GAAGTACAAG GACGAGCCGA CGCGGCTCGT GATCGGCGAT CGCAACACGA TCCGCGAATT CACGACGATC CACACGGGCA CCGTTCAGGA CGCGGGCGTC ACGACGCTCG GCGACGACAA CTGGATCATG GCGTACGTGC ACATCGGTCA CGACTGCCGC GTCGGCAGCC ACGTGGTCCT GTCGAGCAAC GCGCAGATGG CGGGGCACGT CGAGATCGGC GACTGGGCGA TCGTCGGCGG GATGTCGGGC GTTCACCAGT ACGTGCGAAT CGGCGCGCAT TCGATGCTGG GCGGCGCGTC CGCGCTCGTG CAGGACATTC CGCCGTTCGT GATCGCGGCG GGCAACAAGG CCGAGCCGCA CGGGATCAAC GTCGAAGGGC TGCGCCGACG CGGCTTCTCG CCGGACGCAA TTTCCGCGCT GCGCAGCGCG TACCGTATCC TGTACAAGAA CAGCCTGTCG CTCGAAGAGG CGAAGGTCCA GCTGAGCGAG CTCGCGCAGG CGGGCGGCGA CGGCGACGCG GCCGTGAAGG CGCTCGTCGA CTTCGTCGAG TCGTCGCAGC GCGGCATCAT TCGCTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA