ButhA.00325.a

Riboflavin biosynthesis protein RibF (BTH_I0771) (ribF)

CENTER ID: ButhA.00325.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00325.a.A1.GE32794 full length 1 345
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.3488
RefSeq: YP_441327.1
UniProt: Q2T0H2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRHPAALSPT LSPAIVKVFR GLPNAESRAP CALTIGNFDG VHRGHQALLA RVRAAADARG LPVCVMTFEP HPREFFNPAS APPRIAMLRD KLEALREHGV DRVVVEHFNH TFASQSPETF VERTLVHGLH TRWIMVGDDF CYGAKRAGDF ASLKAAGERF GFEVEQMETL ADADGARFSS SGVRAALAAG DLDAAARALG HGYAISGHVT HGLKLGRDLG FPTLNLPIAH KRPALSGIFV VRVHGIGPEP LPGVASLGVR PTVDDSGRVL LEVHLLDWHG DAYGKLVRVE FLKKLRDEAK FVDLETLSRA IAQDVVDTRA YFAEHGRAPG SRATGFATSA TDRIS
NT Sequence
ttgcggcatc cggccgcgct ttcccccacg ctgtcacccg ctatcgtgaa agtctttcgc ggcctgccca acgccgagag ccgcgccccg tgcgcgctga cgatcggcaa cttcgacggt gtccatcgcg gccaccaggc cctgctcgcg cgcgtgcgcg cggcggcgga cgcgcgcggg ctgcccgtgt gcgtgatgac gttcgagccg cacccgcgcg agttcttcaa tccggcgagc gcgccgccgc gcatcgcgat gctgcgcgac aagctcgagg cgctgcgcga gcacggcgtg gaccgcgtgg tcgtcgagca cttcaaccat acgttcgcga gccagtcgcc cgagacgttc gtcgaacgca cgctcgtcca cgggctgcac acccgctgga tcatggtcgg cgacgatttc tgctacggcg cgaaacgcgc gggcgatttc gcgtcgctga aggcggcggg cgagcgcttc ggtttcgagg tcgagcagat ggaaacgctc gccgacgccg acggcgcgcg gttctcgagc tcaggcgtgc gcgccgcgct cgcggcgggg gatctggacg cggccgcgcg agcgctcggc cacggctacg cgatcagcgg ccatgtcacg cacggcctga agctcggccg cgacctcggc ttcccgacgt tgaacctgcc gatcgcgcac aagcgtcccg cgctgtccgg catcttcgtc gtccgcgtgc acggcatcgg gcccgagccg ctgcccggtg tcgcgagcct cggcgtgcgc ccgaccgtcg acgattccgg ccgcgtgctg ctcgaggtgc atctgctcga ctggcacggc gacgcgtacg gcaagctcgt gcgcgtcgaa ttcctgaaga agctgcgcga cgaggcgaaa ttcgtcgatc tcgagacgct ctcgcgcgcg atcgcgcagg acgtcgtcga tacgcgcgcg tacttcgccg agcacggccg tgcgccgggc agccgcgcaa ccggcttcgc gacgtcggcc accgaccgaa ttagctga
Details for ButhA.00325.a.A1.GE32794
HARVESTED ON: 6/28/2011
SEQUENCED ON: 7/22/2011
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MLRHPAALSP TLSPAIVKVF RGLPNAESRA PCALTIGNFD GVHRGHQALL ARVRAAADAR GLPVCVMTFE PHPREFFNPA SAPPRIAMLR DKLEALREHG VDRVVVEHFN HTFASQSPET FVERTLVHGL HTRWIMVGDD FCYGAKRAGD FASLKAAGER FGFEVEQMET LADADGARFS SSGVRAALAA GDLDAAARAL GHGYAISGHV THGLKLGRDL GFPTLNLPIA HKRPALSGIF VVRVHGIGPE PLPGVASLGV RPTVDDSGRV LLEVHLLDWH GDAYGKLVRV EFLKKLRDEA KFVDLETLSR AIAQDVVDTR AYFAEHGRAP GSRATGFATS ATDRIS
Validated NT Sequence
ttattagcta attcggtcgg tggccgacgt cgcgaagccg gttgcgcggc tgcccggcgc acggccgtgc tcggcgaagt acgcgcgcgt atcgacgacg tcctgcgcga tcgcgcgcga gagcgtctcg agatcgacga atttcgcctc gtcgcgcagc ttcttcagga attcgacgcg cacgagcttg ccgtacgcgt cgccgtgcca gtcgagcaga tgcacctcga gcagcacgcg gccggaatcg tcgacggtcg ggcgcacgcc gaggctcgcg acaccgggca gcggctcggg cccgatgccg tgcacgcgga cgacgaagat gccggacagc gcgggacgct tgtgcgcgat cggcaggttc aacgtcggga agccgaggtc gcggccgagc ttcaggccgt gcgtgacatg gccgctgatc gcgtagccgt ggccgagcgc tcgcgcggcc gcgtccagat cccccgccgc gagcgcggcg cgcacgcctg agctcgagaa ccgcgcgccg tcggcgtcgg cgagcgtttc catctgctcg acctcgaaac cgaagcgctc gcccgccgcc ttcagcgacg cgaaatcgcc cgcgcgtttc gcgccgtagc agaaatcgtc gccgaccatg atccagcggg tgtgcagccc gtggacgagc gtgcgttcga cgaacgtctc gggcgactgg ctcgcgaacg tatggttgaa gtgctcgacg accacgcggt ccacgccgtg ctcgcgcagc gcctcgagct tgtcgcgcag catcgcgatg cgcggcggcg cgctcgccgg attgaagaac tcgcgcgggt gcggctcgaa cgtcatcacg cacacgggca gcccgcgcgc gtccgccgcc gcgcgcacgc gcgcgagcag ggcctggtgg ccgcgatgga caccgtcgaa gttgccgatc gtcagcgcgc acggggcgcg gctctcggcg ttgggcaggc cgcgaaagac tttcacgata gcgggtgaca gcgtggggga aagcgcggcc ggatgccgca acatcgaacc aggaccctgg gtctgagctt ccagg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLRHPAALS PTLSPAIVKV FRGLPNAESR APCALTIGNF DGVHRGHQAL LARVRAAADA RGLPVCVMTF EPHPREFFNP ASAPPRIAML RDKLEALREH GVDRVVVEHF NHTFASQSPE TFVERTLVHG LHTRWIMVGD DFCYGAKRAG DFASLKAAGE RFGFEVEQME TLADADGARF SSSGVRAALA AGDLDAAARA LGHGYAISGH VTHGLKLGRD LGFPTLNLPI AHKRPALSGI FVVRVHGIGP EPLPGVASLG VRPTVDDSGR VLLEVHLLDW HGDAYGKLVR VEFLKKLRDE AKFVDLETLS RAIAQDVVDT RAYFAEHGRA PGSRATGFAT SATDRIS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgcggcat ccggccgcgc tttcccccac gctgtcaccc gctatcgtga aagtctttcg cggcctgccc aacgccgaga gccgcgcccc gtgcgcgctg acgatcggca acttcgacgg tgtccatcgc ggccaccagg ccctgctcgc gcgcgtgcgc gcggcggcgg acgcgcgcgg gctgcccgtg tgcgtgatga cgttcgagcc gcacccgcgc gagttcttca atccggcgag cgcgccgccg cgcatcgcga tgctgcgcga caagctcgag gcgctgcgcg agcacggcgt ggaccgcgtg gtcgtcgagc acttcaacca tacgttcgcg agccagtcgc ccgagacgtt cgtcgaacgc acgctcgtcc acgggctgca cacccgctgg atcatggtcg gcgacgattt ctgctacggc gcgaaacgcg cgggcgattt cgcgtcgctg aaggcggcgg gcgagcgctt cggtttcgag gtcgagcaga tggaaacgct cgccgacgcc gacggcgcgc ggttctcgag ctcaggcgtg cgcgccgcgc tcgcggcggg ggatctggac gcggccgcgc gagcgctcgg ccacggctac gcgatcagcg gccatgtcac gcacggcctg aagctcggcc gcgacctcgg cttcccgacg ttgaacctgc cgatcgcgca caagcgtccc gcgctgtccg gcatcttcgt cgtccgcgtg cacggcatcg ggcccgagcc gctgcccggt gtcgcgagcc tcggcgtgcg cccgaccgtc gacgattccg gccgcgtgct gctcgaggtg catctgctcg actggcacgg cgacgcgtac ggcaagctcg tgcgcgtcga attcctgaag aagctgcgcg acgaggcgaa attcgtcgat ctcgagacgc tctcgcgcgc gatcgcgcag gacgtcgtcg atacgcgcgc gtacttcgcc gagcacggcc gtgcgccggg cagccgcgca accggcttcg cgacgtcggc caccgaccga attagctaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa