ButhA.00317.a: Triosephosphate isomerase (TIM) (EC 5.3.1.1) (Triose-phosphate isomerase) (BTH_I1058) (tpiA)
Target Characteristics
| from organism: | Burkholderia thailandensis E264 |
| most recent status: | in PDB |
| center reference id: | ButhA.00317.a |
| is community request: | True |
| associated disease: | |
| NIH risk group: | 3 |
| is select agent: | False |
| NIH priority pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00317.a.A1.GE32736 | full length | 1 | 251 | GE32736 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00317.a.A1.PS01252 | full length | 1 | 251 |
Structures
4G1K
Deposited: 7/10/2012
Determination: XRay
Diffraction Image
Clone: ButhA.00317.a.A1.GE32736
Protein: ButhA.00317.a.A1.PS01252
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| Resource | Reference ID |
|---|---|
| OrthoMCL: | OG5_127002 |
| PATRIC ID: | fig|271848.6.peg.3819 |
| RefSeq: | YP_441612.1 |
| UniProt: | Q2SZN7 |
Enzyme & Pathway Information
| Pathway | Pathway ID | EC Number |
|---|---|---|
| homolactic fermentation | ANAEROFRUCAT-PWY | 5.3.1.1 |
| glycolysis III (from glucose) | ANAGLYCOLYSIS-PWY | 5.3.1.1 |
| Calvin-Benson-Bassham cycle | CALVIN-PWY | 5.3.1.1 |
| gluconeogenesis I | GLUCONEO-PWY | 5.3.1.1 |
| glycolysis I (from glucose 6-phosphate) | GLYCOLYSIS | 5.3.1.1 |
| superpathway of glycolysis and the Entner-Doudoroff pathway | GLYCOLYSIS-E-D | 5.3.1.1 |
| superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass | GLYCOLYSIS-TCA-GLYOX-BYPASS | 5.3.1.1 |
| superpathway of hexitol degradation (bacteria) | HEXITOLDEGSUPER-PWY | 5.3.1.1 |
| formaldehyde assimilation III (dihydroxyacetone cycle) | P185-PWY | 5.3.1.1 |
| glycolysis V (Pyrococcus) | P341-PWY | 5.3.1.1 |
| superpathway of N-acetylneuraminate degradation | P441-PWY | 5.3.1.1 |
| hexitol fermentation to lactate, formate, ethanol and acetate | P461-PWY | 5.3.1.1 |
| oxygenic photosynthesis | PHOTOALL-PWY | 5.3.1.1 |
| glycolysis IV (plant cytosol) | PWY-1042 | 5.3.1.1 |
| superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle | PWY-5464 | 5.3.1.1 |
| glycolysis II (from fructose 6-phosphate) | PWY-5484 | 5.3.1.1 |
| gluconeogenesis II (Methanobacterium thermoautotrophicum) | PWY-6142 | 5.3.1.1 |
| Methanobacterium thermoautotrophicum biosynthetic metabolism | PWY-6146 | 5.3.1.1 |
| sucrose degradation V (sucrose alpha-glucosidase) | PWY66-373 | 5.3.1.1 |
| gluconeogenesis III | PWY66-399 | 5.3.1.1 |
| glycolysis VI (metazoan) | PWY66-400 | 5.3.1.1 |
| 1-butanol autotrophic biosynthesis (engineered) | PWY-6886 | 5.3.1.1 |
| glycerol degradation to butanol | PWY-7003 | 5.3.1.1 |
| ethylene biosynthesis V (engineered) | PWY-7124 | 5.3.1.1 |
| photosynthetic 3-hydroxybutanoate biosynthesis (engineered) | PWY-7218 | 5.3.1.1 |
| superpathway of anaerobic sucrose degradation | PWY-7345 | 5.3.1.1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MSKQRIKRVI GNWKMHGRLS GNQALLTEVA QGAQAVHDNV AIGVCVPFPY LAQAQAQLQG GRVSWGSQDV SAHEQGAYTG EVAAGMVAEF GAAYAIVGHS ERRAYHGESN ETVAAKARRA LAAGLTPIVC VGETLAEREA GTTEQVVGAQ LDAVLAVLSP DEAARIVVAY EPVWAIGTGK SATAEQAQQV HAFLRGRLAA KGAGHVSLLY GGSVKADNAA ELFGQPDIDG GLIGGASLKS GDFLAICRAA K
NT Sequence
atgtcgaaac agagaatcaa gcgggtgatc ggcaactgga agatgcacgg tcgcctgagc ggcaaccagg cattgctgac ggaagtcgcg cagggcgcgc aagcggtaca tgacaacgtc gccatcggcg tgtgcgtgcc gttcccatat ctcgcgcagg cgcaggctca gcttcagggc ggtcgcgtct cgtggggctc gcaggacgtg tccgcgcacg agcagggcgc ctacaccggc gaagtcgcgg ccggcatggt ggcggagttc ggcgcggcgt acgcgatcgt cgggcactcg gagcgccgcg cgtatcacgg cgaatcgaac gagaccgtcg cggcgaaggc gcggcgcgcg ctcgctgcgg ggttgacgcc catcgtgtgc gtcggcgaga cgcttgccga gcgcgaagcg ggcacgaccg agcaggtggt cggcgcgcag ctcgatgcgg tgctcgcggt gctgtcgccg gacgaggcgg cgcgcatcgt cgtcgcatac gagcccgtat gggccatcgg cacgggcaag agcgcgacgg ccgagcaggc gcagcaggtg catgcgttcc tgcgcggacg gctcgcggcg aagggcgccg ggcacgtgtc gctgttgtat ggcggcagcg tgaaggccga caacgctgcc gagctgttcg gccagccgga catcgacggc ggcctgattg gcggcgcgtc gttgaagagc ggggatttcc tggcgatctg ccgggcagcg aagtaa