ButhA.00211.a

Pyrroline-5-carboxylate reductase (EC 1.5.1.2) (BTH_I1287) (proC)

CENTER ID: ButhA.00211.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00211.a.A1.GE32790 full length 1 270
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00211.a.A1.PW33394 full length 1 270

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.4051
RefSeq: YP_441834.1
UniProt: Q2SZ15

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKIAFIGGGN MAAALIGGLV RRGVAASELL AVDINEDARQ RAQAQFGVRT AAAIDATLAG YDAIVLAVKP QVLKEIATAL APHLSTQLVV SIAAGIRGAD LSRWLGDYAR VVRTMPNTPA LVGLGVTGLS ALPGVDAAGR DLASKVLGAV GETVWFDDES QLDAVTAISG SGPAYVFYFI EALQEAARQL GMNDEQGRAL AVATFAGAAQ LAVQSGEPAS VLRERVTSKG GTTAAALASF DAQHVKDSIV RGVLAAQARA KEMGDELGAA
NT Sequence
atgaaaatcg cattcatcgg cggcggcaac atggcggccg cgttgatcgg cggactcgtc aggcgcggcg tcgcggcgag cgagctgctc gccgtcgaca tcaacgaaga tgcgcgtcag cgcgcgcaag cgcaattcgg cgtgcgcacg gcggccgcca tcgacgccac gctcgccggc tacgacgcga tcgtgctcgc cgtgaagccg caggtgctca aggagatcgc gaccgcgctc gcgccgcatc tgtcgacaca gctcgtcgtc agcatcgcgg ccggcattcg cggcgcggac ctgtcgcgct ggctcggcga ttacgcgcgc gtcgtgcgca cgatgccgaa cacgccggcg ctcgtcggcc tcggcgtgac cggcctgtcc gcactgccgg gcgtcgacgc ggcgggccgc gatctcgcat cgaaggtgct cggcgcggtc ggcgagacgg tctggttcga cgacgaatcg cagctcgacg ccgtcaccgc gatctcgggc agcggccccg cgtacgtgtt ctatttcatc gaggcgctgc aggaagcggc gcgccagctc ggcatgaacg acgagcaggg ccgcgcgctc gcggtcgcga cgttcgcggg cgccgcgcag ctcgcggtgc agtcgggcga gcccgcgagc gtgctgcgcg agcgcgtgac gtcgaagggc ggcacgacgg cggccgcgct ggcgtcgttc gacgcgcaac acgtgaagga ctcgatcgtg cgcggcgtgc tcgccgcaca ggcgcgcgcg aaggaaatgg gcgacgagct gggcgctgcg taa
Details for ButhA.00211.a.A1.GE32790
HARVESTED ON: 6/28/2011
SEQUENCED ON: 7/22/2011
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKIAFIGGG NMAAALIGGL VRRGVAASEL LAVDINEDAR QRAQAQFGVR TAAAIDATLA GYDAIVLAVK PQVLKEIATA LAPHLSTQLV VSIAAGIRGA DLSRWLGDYA RVVRTMPNTP ALVGLGVTGL SALPGVDAAG RDLASKVLGA VGETVWFDDE SQLDAVTAIS GSGPAYVFYF IEALQEAARQ LGMNDEQGRA LAVATFAGAA QLAVQSGEPA SVLRERVTSK GGTTAAALAS FDAQHVKDSI VRGVLAAQAR AKEMGDELGA A
Validated NT Sequence
ttattacgca gcgcccagct cgtcgcccat ttccttcgcg cgcgcctgtg cggcgagcac gccgcgcacg atcgagtcct tcacgtgttg cgcgtcgaac gacgccagcg cggccgccgt cgtgccgccc ttcgacgtca cgcgctcgcg cagcacgctc gcgggctcgc ccgactgcac cgcgagctgc gcggcgcccg cgaacgtcgc gaccgcgagc gcgcggccct gctcgtcgtt catgccgagc tggcgcgccg cttcctgcag cgcctcgatg aaatagaaca cgtacgcggg gccgctgccc gagatcgcgg tgacggcgtc gagctgcgat tcgtcgtcga accagaccgt ctcgccgacc gcgccgagca ccttcgatgc gagatcgcgg cccgccgcgt cgacgcccgg cagtgcggac aggccggtca cgccgaggcc gacgagcgcc ggcgtgttcg gcatcgtgcg cacgacgcgc gcgtaatcgc cgagccagcg cgacaggtcc gcgccgcgaa tgccggccgc gatgctgacg acgagctgtg tcgacagatg cggcgcgagc gcggtcgcga tctccttgag cacctgcggc ttcacggcga gcacgatcgc gtcgtagccg gcgagcgtgg cgtcgatggc ggccgccgtg cgcacgccga attgcgcttg cgcgcgctga cgcgcatctt cgttgatgtc gacggcgagc agctcgctcg ccgcgacgcc gcgcctgacg agtccgccga tcaacgcggc cgccatgttg ccgccgccga tgaatgcgat tttcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKIAFIGGG NMAAALIGGL VRRGVAASEL LAVDINEDAR QRAQAQFGVR TAAAIDATLA GYDAIVLAVK PQVLKEIATA LAPHLSTQLV VSIAAGIRGA DLSRWLGDYA RVVRTMPNTP ALVGLGVTGL SALPGVDAAG RDLASKVLGA VGETVWFDDE SQLDAVTAIS GSGPAYVFYF IEALQEAARQ LGMNDEQGRA LAVATFAGAA QLAVQSGEPA SVLRERVTSK GGTTAAALAS FDAQHVKDSI VRGVLAAQAR AKEMGDELGA A
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaaatcgca ttcatcggcg gcggcaacat ggcggccgcg ttgatcggcg gactcgtcag gcgcggcgtc gcggcgagcg agctgctcgc cgtcgacatc aacgaagatg cgcgtcagcg cgcgcaagcg caattcggcg tgcgcacggc ggccgccatc gacgccacgc tcgccggcta cgacgcgatc gtgctcgccg tgaagccgca ggtgctcaag gagatcgcga ccgcgctcgc gccgcatctg tcgacacagc tcgtcgtcag catcgcggcc ggcattcgcg gcgcggacct gtcgcgctgg ctcggcgatt acgcgcgcgt cgtgcgcacg atgccgaaca cgccggcgct cgtcggcctc ggcgtgaccg gcctgtccgc actgccgggc gtcgacgcgg cgggccgcga tctcgcatcg aaggtgctcg gcgcggtcgg cgagacggtc tggttcgacg acgaatcgca gctcgacgcc gtcaccgcga tctcgggcag cggccccgcg tacgtgttct atttcatcga ggcgctgcag gaagcggcgc gccagctcgg catgaacgac gagcagggcc gcgcgctcgc ggtcgcgacg ttcgcgggcg ccgcgcagct cgcggtgcag tcgggcgagc ccgcgagcgt gctgcgcgag cgcgtgacgt cgaagggcgg cacgacggcg gccgcgctgg cgtcgttcga cgcgcaacac gtgaaggact cgatcgtgcg cggcgtgctc gccgcacagg cgcgcgcgaa ggaaatgggc gacgagctgg gcgctgcgta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for ButhA.00211.a.A1.PW33394
PURIFICATION DATe: 8/19/2011
CONCENTRATION: 34.32mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 15
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKIAFIGGG NMAAALIGGL VRRGVAASEL LAVDINEDAR QRAQAQFGVR TAAAIDATLA GYDAIVLAVK PQVLKEIATA LAPHLSTQLV VSIAAGIRGA DLSRWLGDYA RVVRTMPNTP ALVGLGVTGL SALPGVDAAG RDLASKVLGA VGETVWFDDE SQLDAVTAIS GSGPAYVFYF IEALQEAARQ LGMNDEQGRA LAVATFAGAA QLAVQSGEPA SVLRERVTSK GGTTAAALAS FDAQHVKDSI VRGVLAAQAR AKEMGDELGA A
Validated NT Sequence
ttattacgca gcgcccagct cgtcgcccat ttccttcgcg cgcgcctgtg cggcgagcac gccgcgcacg atcgagtcct tcacgtgttg cgcgtcgaac gacgccagcg cggccgccgt cgtgccgccc ttcgacgtca cgcgctcgcg cagcacgctc gcgggctcgc ccgactgcac cgcgagctgc gcggcgcccg cgaacgtcgc gaccgcgagc gcgcggccct gctcgtcgtt catgccgagc tggcgcgccg cttcctgcag cgcctcgatg aaatagaaca cgtacgcggg gccgctgccc gagatcgcgg tgacggcgtc gagctgcgat tcgtcgtcga accagaccgt ctcgccgacc gcgccgagca ccttcgatgc gagatcgcgg cccgccgcgt cgacgcccgg cagtgcggac aggccggtca cgccgaggcc gacgagcgcc ggcgtgttcg gcatcgtgcg cacgacgcgc gcgtaatcgc cgagccagcg cgacaggtcc gcgccgcgaa tgccggccgc gatgctgacg acgagctgtg tcgacagatg cggcgcgagc gcggtcgcga tctccttgag cacctgcggc ttcacggcga gcacgatcgc gtcgtagccg gcgagcgtgg cgtcgatggc ggccgccgtg cgcacgccga attgcgcttg cgcgcgctga cgcgcatctt cgttgatgtc gacggcgagc agctcgctcg ccgcgacgcc gcgcctgacg agtccgccga tcaacgcggc cgccatgttg ccgccgccga tgaatgcgat tttcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKIAFIGGG NMAAALIGGL VRRGVAASEL LAVDINEDAR QRAQAQFGVR TAAAIDATLA GYDAIVLAVK PQVLKEIATA LAPHLSTQLV VSIAAGIRGA DLSRWLGDYA RVVRTMPNTP ALVGLGVTGL SALPGVDAAG RDLASKVLGA VGETVWFDDE SQLDAVTAIS GSGPAYVFYF IEALQEAARQ LGMNDEQGRA LAVATFAGAA QLAVQSGEPA SVLRERVTSK GGTTAAALAS FDAQHVKDSI VRGVLAAQAR AKEMGDELGA A
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAAATCGCA TTCATCGGCG GCGGCAACAT GGCGGCCGCG TTGATCGGCG GACTCGTCAG GCGCGGCGTC GCGGCGAGCG AGCTGCTCGC CGTCGACATC AACGAAGATG CGCGTCAGCG CGCGCAAGCG CAATTCGGCG TGCGCACGGC GGCCGCCATC GACGCCACGC TCGCCGGCTA CGACGCGATC GTGCTCGCCG TGAAGCCGCA GGTGCTCAAG GAGATCGCGA CCGCGCTCGC GCCGCATCTG TCGACACAGC TCGTCGTCAG CATCGCGGCC GGCATTCGCG GCGCGGACCT GTCGCGCTGG CTCGGCGATT ACGCGCGCGT CGTGCGCACG ATGCCGAACA CGCCGGCGCT CGTCGGCCTC GGCGTGACCG GCCTGTCCGC ACTGCCGGGC GTCGACGCGG CGGGCCGCGA TCTCGCATCG AAGGTGCTCG GCGCGGTCGG CGAGACGGTC TGGTTCGACG ACGAATCGCA GCTCGACGCC GTCACCGCGA TCTCGGGCAG CGGCCCCGCG TACGTGTTCT ATTTCATCGA GGCGCTGCAG GAAGCGGCGC GCCAGCTCGG CATGAACGAC GAGCAGGGCC GCGCGCTCGC GGTCGCGACG TTCGCGGGCG CCGCGCAGCT CGCGGTGCAG TCGGGCGAGC CCGCGAGCGT GCTGCGCGAG CGCGTGACGT CGAAGGGCGG CACGACGGCG GCCGCGCTGG CGTCGTTCGA CGCGCAACAC GTGAAGGACT CGATCGTGCG CGGCGTGCTC GCCGCACAGG CGCGCGCGAA GGAAATGGGC GACGAGCTGG GCGCTGCGTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A