SSGCID: Target Page

ButhA.00171.c

3-oxoacyl-[acyl-carrier-protein] synthase

CENTER ID:	ButhA.00171.c
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00171.c.A1.GE32422	Full length( ButhA.00171.c )		1	332

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00171.c.A1.PS01334	Full length( ButhA.00171.c )		1	332

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.2149
RefSeq:	YP_440126.1
UniProt:	Q2T3X3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPHNLEIAGL GHALPERIVG NDEVARVIDT SDAFIRTRTG VRHRRYLAPG QQLADLACPA AERAMADAGV APADIDLLIV NTLSPDHHDP SQACYMQPRL GLREIPCFDI RAQCSGGLYG IEIARHFVAS GLYRNVLLIC AEALSRRIDT SNAGRNLSIL LSDGAAAMVL RAQPDATRGL IDLALGADGT QFDLLQTEAP GAKRPNFIDA DDIAAGRHYF RMRGADMFED ATRRIVDACR QMLDKHRLAL GDIGLVVPHQ PNLRIIDAVI EQLGLPRERC MISVEDLGNM ASAAFPVALA LAHEQGRMPA GQLNLLVTYG AGATWACALY RS

NT Sequence

ATGCCGCATA ACCTCGAGAT CGCCGGGCTT GGCCATGCGC TGCCGGAGCG GATTGTCGGC AACGACGAAG TCGCGCGCGT CATCGACACG AGCGACGCGT TCATCCGCAC GCGCACCGGC GTGCGGCATC GCCGCTACCT CGCGCCCGGC CAGCAGCTCG CGGACCTCGC CTGCCCGGCC GCCGAGCGTG CGATGGCCGA CGCGGGCGTC GCGCCCGCCG ACATCGACCT GCTGATCGTC AACACGCTGT CGCCCGATCA CCACGATCCG TCGCAGGCGT GCTACATGCA GCCGCGGCTC GGGCTGCGCG AGATTCCGTG CTTCGACATC CGCGCGCAAT GCAGCGGCGG ACTGTACGGC ATCGAGATCG CGCGGCACTT CGTCGCGAGC GGCCTGTATC GCAACGTGCT GCTGATCTGC GCGGAAGCGC TGTCGCGGCG GATCGACACG AGCAACGCGG GCCGCAACCT GTCGATCCTG CTGAGCGACG GCGCGGCCGC GATGGTGCTG CGTGCGCAGC CCGACGCCAC GCGCGGCCTC ATCGACCTCG CGCTCGGCGC GGACGGCACG CAGTTCGATC TGCTGCAGAC GGAGGCGCCG GGCGCGAAGC GGCCGAACTT CATCGACGCC GACGACATCG CGGCGGGCCG CCACTACTTC CGGATGCGCG GCGCCGACAT GTTCGAGGAC GCGACGCGCC GCATCGTCGA CGCGTGCCGG CAGATGCTCG ACAAGCATCG GCTCGCGCTC GGCGATATCG GGCTCGTCGT GCCGCATCAG CCGAACCTGC GGATCATCGA CGCGGTGATC GAGCAGCTCG GCCTGCCGCG CGAGCGCTGC ATGATTTCCG TCGAGGACCT CGGCAACATG GCGAGCGCCG CGTTTCCGGT CGCACTCGCG CTCGCGCACG AGCAGGGGCG GATGCCGGCC GGCCAGTTGA ACCTGCTCGT GACCTACGGC GCGGGCGCGA CCTGGGCGTG CGCGCTTTAT CGGAGC

Details for ButhA.00171.c.A1.GE32422

HARVESTED ON:	11/8/2011
SEQUENCED ON:	11/11/2011
EXPECTED MW:	38kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

ttattagctc cgataaagcg cgcacgccca ggtcgcgccc gcgccgtagg tcacgagcag gttcaactgg ccggccggca tccgcccctg ctcgtgcgcg agcgcgagtg cgaccggaaa cgcggcgctc gccatgttgc cgaggtcctc gacggaaatc atgcagcgct cgcgcggcag gccgagctgc tcgatcaccg cgtcgatgat ccgcaggttc ggctgatgcg gcacgacgag cccgatatcg ccgagcgcga gccgatgctt gtcgagcatc tgccggcacg cgtcgacgat gcggcgcgtc gcgtcctcga acatgtcggc gccgcgcatc cggaagtagt ggcggcccgc cgcgatgtcg tcggcgtcga tgaagttcgg ccgcttcgcg cccggcgcct ccgtctgcag cagatcgaac tgcgtgccgt ccgcgccgag cgcgaggtcg atgaggccgc gcgtggcgtc gggctgcgca cgcagcacca tcgcggccgc gccgtcgctc agcaggatcg acaggttgcg gcccgcgttg ctcgtgtcga tccgccgcga cagcgcttcc gcgcagatca gcagcacgtt gcgatacagg ccgctcgcga cgaagtgccg cgcgatctcg atgccgtaca gtccgccgct gcattgcgcg cggatgtcga agcacggaat ctcgcgcagc ccgagccgcg gctgcatgta gcacgcctgc gacggatcgt ggtgatcggg cgacagcgtg ttgacgatca gcaggtcgat gtcggcgggc gcgacgcccg cgtcggccat cgcacgctcg gcggccgggc aggcgaggtc cgcgagctgc tggccgggcg cgaggtagcg gcgatgccgc acgccggtgc gtgtgcggat gaacgcgtcg ctcgtgtcga tgacgcgcgc gacttcgtcg ttgccgacaa tccgctccgg cagcgcatgg ccaagcccgg cgatctcgag gttatgcggc atcgaaccag gaccctgggt ctgagcttcc agggtacc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMPHNLEIAG LGHALPERIV GNDEVARVID TSDAFIRTRT GVRHRRYLAP GQQLADLACP AAERAMADAG VAPADIDLLI VNTLSPDHHD PSQACYMQPR LGLREIPCFD IRAQCSGGLY GIEIARHFVA SGLYRNVLLI CAEALSRRID TSNAGRNLSI LLSDGAAAMV LRAQPDATRG LIDLALGADG TQFDLLQTEA PGAKRPNFID ADDIAAGRHY FRMRGADMFE DATRRIVDAC RQMLDKHRLA LGDIGLVVPH QPNLRIIDAV IEQLGLPRER CMISVEDLGN MASAAFPVAL ALAHEQGRMP AGQLNLLVTY GAGATWACAL YRS

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccgcataac ctcgagatcg ccgggcttgg ccatgcgctg ccggagcgga ttgtcggcaa cgacgaagtc gcgcgcgtca tcgacacgag cgacgcgttc atccgcacgc gcaccggcgt gcggcatcgc cgctacctcg cgcccggcca gcagctcgcg gacctcgcct gcccggccgc cgagcgtgcg atggccgacg cgggcgtcgc gcccgccgac atcgacctgc tgatcgtcaa cacgctgtcg cccgatcacc acgatccgtc gcaggcgtgc tacatgcagc cgcggctcgg gctgcgcgag attccgtgct tcgacatccg cgcgcaatgc agcggcggac tgtacggcat cgagatcgcg cggcacttcg tcgcgagcgg cctgtatcgc aacgtgctgc tgatctgcgc ggaagcgctg tcgcggcgga tcgacacgag caacgcgggc cgcaacctgt cgatcctgct gagcgacggc gcggccgcga tggtgctgcg tgcgcagccc gacgccacgc gcggcctcat cgacctcgcg ctcggcgcgg acggcacgca gttcgatctg ctgcagacgg aggcgccggg cgcgaagcgg ccgaacttca tcgacgccga cgacatcgcg gcgggccgcc actacttccg gatgcgcggc gccgacatgt tcgaggacgc gacgcgccgc atcgtcgacg cgtgccggca gatgctcgac aagcatcggc tcgcgctcgg cgatatcggg ctcgtcgtgc cgcatcagcc gaacctgcgg atcatcgacg cggtgatcga gcagctcggc ctgccgcgcg agcgctgcat gatttccgtc gaggacctcg gcaacatggc gagcgccgcg tttccggtcg cactcgcgct cgcgcacgag caggggcgga tgccggccgg ccagttgaac ctgctcgtga cctacggcgc gggcgcgacc tgggcgtgcg cgctttatcg gagctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for ButhA.00171.c.A1.PS01334

PURIFICATION DATe:	1/10/2012
CONCENTRATION:	19.5mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCGCATAAC CTCGAGATCG CCGGGCTTGG CCATGCGCTG CCGGAGCGGA TTGTCGGCAA CGACGAAGTC GCGCGCGTCA TCGACACGAG CGACGCGTTC ATCCGCACGC GCACCGGCGT GCGGCATCGC CGCTACCTCG CGCCCGGCCA GCAGCTCGCG GACCTCGCCT GCCCGGCCGC CGAGCGTGCG ATGGCCGACG CGGGCGTCGC GCCCGCCGAC ATCGACCTGC TGATCGTCAA CACGCTGTCG CCCGATCACC ACGATCCGTC GCAGGCGTGC TACATGCAGC CGCGGCTCGG GCTGCGCGAG ATTCCGTGCT TCGACATCCG CGCGCAATGC AGCGGCGGAC TGTACGGCAT CGAGATCGCG CGGCACTTCG TCGCGAGCGG CCTGTATCGC AACGTGCTGC TGATCTGCGC GGAAGCGCTG TCGCGGCGGA TCGACACGAG CAACGCGGGC CGCAACCTGT CGATCCTGCT GAGCGACGGC GCGGCCGCGA TGGTGCTGCG TGCGCAGCCC GACGCCACGC GCGGCCTCAT CGACCTCGCG CTCGGCGCGG ACGGCACGCA GTTCGATCTG CTGCAGACGG AGGCGCCGGG CGCGAAGCGG CCGAACTTCA TCGACGCCGA CGACATCGCG GCGGGCCGCC ACTACTTCCG GATGCGCGGC GCCGACATGT TCGAGGACGC GACGCGCCGC ATCGTCGACG CGTGCCGGCA GATGCTCGAC AAGCATCGGC TCGCGCTCGG CGATATCGGG CTCGTCGTGC CGCATCAGCC GAACCTGCGG ATCATCGACG CGGTGATCGA GCAGCTCGGC CTGCCGCGCG AGCGCTGCAT GATTTCCGTC GAGGACCTCG GCAACATGGC GAGCGCCGCG TTTCCGGTCG CACTCGCGCT CGCGCACGAG CAGGGGCGGA TGCCGGCCGG CCAGTTGAAC CTGCTCGTGA CCTACGGCGC GGGCGCGACC TGGGCGTGCG CGCTTTATCG GAGCTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA