SSGCID: Target Page

ButhA.00171.b

Putative uncharacterized protein

CENTER ID:	ButhA.00171.b
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00171.b.A1.GE32421	Full length( ButhA.00171.b )		1	368

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.2150
RefSeq:	YP_440127.1
UniProt:	Q2T3X2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSYNVRMASL ACNLPSRLLR NDDAAFDDVE PIPSEWWQFW GIESRYVIDP HAGESELAMA ERTARQALAR AGVDAGELDL VLFNITSPFV TADDGARRFA PRLSRTLRER LGARSALDAD VEMECASFVL QMQLATNLIK QGRVKRALVC SSERMSAVVD YTSKSSTTFG DGAAAAVLVA DAHDPHGADW LNAVYRSDAT HYDLATMQWR YPRPAADRDD AERQAERFGA YFTLKPEAQE QIARFMPEAI PDIVERLLRK TGLRADDIDA MVFHQPSDIL VRAWAQRLGL SPDQYVIRLA DCACLVSAAV PLALYESLRR GVVKPGSQVV IAGAGAGWGF GAQLWRWGET VVTDAAPTVE AAESEHAA

NT Sequence

ATGTCCTACA ACGTTCGCAT GGCCAGTCTC GCGTGCAATC TGCCGTCGCG CTTGCTGCGC AACGACGATG CCGCATTCGA CGACGTCGAG CCGATTCCGT CCGAATGGTG GCAGTTCTGG GGAATCGAAT CCCGCTACGT GATCGATCCG CACGCGGGCG AATCCGAGCT CGCGATGGCC GAGCGCACCG CGCGCCAGGC GCTCGCGCGC GCGGGCGTCG ATGCGGGCGA GCTCGATCTC GTGCTGTTCA ACATCACGTC ACCGTTCGTC ACGGCGGACG ACGGCGCGCG GCGCTTCGCG CCGCGGCTGT CGCGCACGCT GCGCGAGCGG CTCGGCGCGC GCAGCGCGCT TGACGCCGAT GTCGAGATGG AATGCGCGAG CTTCGTGCTG CAAATGCAGC TCGCGACGAA CCTGATCAAG CAGGGGCGCG TGAAGCGCGC GCTCGTGTGC TCGTCCGAGC GGATGTCGGC GGTCGTCGAT TACACGAGCA AGTCGTCGAC GACGTTCGGC GACGGCGCGG CGGCCGCCGT GCTCGTCGCC GACGCGCACG ATCCGCACGG CGCCGACTGG CTGAATGCCG TCTATCGCAG CGACGCGACG CACTACGATC TCGCGACGAT GCAATGGCGC TACCCGAGGC CCGCGGCGGA CCGCGACGAC GCCGAGCGCC AGGCCGAGCG CTTCGGCGCG TATTTCACGC TGAAGCCCGA GGCGCAGGAG CAGATCGCGC GCTTCATGCC CGAGGCGATC CCCGACATCG TCGAGCGCCT GCTGCGCAAG ACGGGGCTGC GCGCCGACGA TATCGACGCG ATGGTGTTCC ATCAGCCGTC GGATATCCTC GTGCGCGCGT GGGCGCAGCG TCTCGGCCTC TCGCCGGATC AATACGTGAT TCGCCTGGCC GACTGCGCGT GCCTCGTGTC GGCTGCGGTG CCGCTCGCGC TCTACGAATC GCTTCGCCGC GGCGTCGTGA AGCCGGGCTC GCAAGTGGTG ATCGCGGGCG CGGGCGCGGG CTGGGGCTTC GGCGCGCAGC TGTGGCGCTG GGGCGAGACG GTCGTGACCG ACGCGGCGCC GACGGTCGAG GCGGCGGAGA GCGAACATGC CGCA

Details for ButhA.00171.b.A1.GE32421

HARVESTED ON:	11/8/2011
SEQUENCED ON:	11/11/2011
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	65

Validated AA Sequence

Validated NT Sequence

attttgttta actttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgtcc tacaacgttc gcatggccag tctcgcgtgc aatctgccgt cgcgcttgct gcgcaacgac gatgccgcat tcgacgacgt cgagccgatt ccgtccgaat ggtggcagtt ctggggaatc gaatcccgct acgtgatcga tccgcacgcg ggcgaatccg agctcgcgat ggccgagcgc accgcgcgcc aggcgctcgc gcgcgcgggc gtcgatgcgg gcgagctcga tctcgtgctg ttcaacatca cgtcaccgtt cgtcacggcg gacgacggcg cgcggcgctt cgcgccgcgg ctgtcgcgca cgctgcgcga gcggctcggc gcgcgcagcg cgcttgacgc cgatgtcgag atggaatgcg cgagcttcgt gctgcaaatg cagctcgcga cgaacctgat caagcagggg cgcgtgaagc gcgcgctcgt gtgctcgtcc gagcggatgt cggcggtcgt cgattacacg agcaagtcgt cgacgacgtt cggcgacggc gcggcggccg ccgtgctcgt cgccgacgcg cacgatccgc acggcgccga ctggctgaat gccgtctatc gcagcgacgc gacgcactac gatctcgcga cgatgcaatg gcgctacccg aggcccgcgg cggaccgcga cgacgccnag cgccaggncg agcgcttcgc gcgtatttca cgctgaagcc cgaggcgcag gagcagatcg cgcgcttcat gcccgangcg atccccgaca tcgtcnagcg cctgctgcgc aagacggggc tgcgcgccga cgatatcgac gcgatggtgt tccatcagcc gtcggatatc ctcgtgcgcg cgtgggcgca gcgtctcgnn ctctcgnnng atcaatactg attcncctng gccgactgcg nntgcctcgn gtcggctgnn ngcnctcgcg ctctacnanc gcttcnnnnc ngcgtcnnna ncngnntnnn nnnggngatc nnngggnnnn ggnnnngnnt ggggnntngn nnnncnnnnt nnnnnnctgg gnnnnnnnnn ncgtnacnna nnnnnnnnnc nnacnnnnnn nnngnnnnnn nnnatnnnnn nnannnnnnn nnnnnnnnnn nnnnannnnn nnntnnnnnn nnnnnnnnnn nntnnnaa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSYNVRMAS LACNLPSRLL RNDDAAFDDV EPIPSEWWQF WGIESRYVID PHAGESELAM AERTARQALA RAGVDAGELD LVLFNITSPF VTADDGARRF APRLSRTLRE RLGARSALDA DVEMECASFV LQMQLATNLI KQGRVKRALV CSSERMSAVV DYTSKSSTTF GDGAAAAVLV ADAHDPHGAD WLNAVYRSDA THYDLATMQW RYPRPAADRD DAERQAERFG AYFTLKPEAQ EQIARFMPEA IPDIVERLLR KTGLRADDID AMVFHQPSDI LVRAWAQRLG LSPDQYVIRL ADCACLVSAA VPLALYESLR RGVVKPGSQV VIAGAGAGWG FGAQLWRWGE TVVTDAAPTV EAAESEHAA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcctacaac gttcgcatgg ccagtctcgc gtgcaatctg ccgtcgcgct tgctgcgcaa cgacgatgcc gcattcgacg acgtcgagcc gattccgtcc gaatggtggc agttctgggg aatcgaatcc cgctacgtga tcgatccgca cgcgggcgaa tccgagctcg cgatggccga gcgcaccgcg cgccaggcgc tcgcgcgcgc gggcgtcgat gcgggcgagc tcgatctcgt gctgttcaac atcacgtcac cgttcgtcac ggcggacgac ggcgcgcggc gcttcgcgcc gcggctgtcg cgcacgctgc gcgagcggct cggcgcgcgc agcgcgcttg acgccgatgt cgagatggaa tgcgcgagct tcgtgctgca aatgcagctc gcgacgaacc tgatcaagca ggggcgcgtg aagcgcgcgc tcgtgtgctc gtccgagcgg atgtcggcgg tcgtcgatta cacgagcaag tcgtcgacga cgttcggcga cggcgcggcg gccgccgtgc tcgtcgccga cgcgcacgat ccgcacggcg ccgactggct gaatgccgtc tatcgcagcg acgcgacgca ctacgatctc gcgacgatgc aatggcgcta cccgaggccc gcggcggacc gcgacgacgc cgagcgccag gccgagcgct tcggcgcgta tttcacgctg aagcccgagg cgcaggagca gatcgcgcgc ttcatgcccg aggcgatccc cgacatcgtc gagcgcctgc tgcgcaagac ggggctgcgc gccgacgata tcgacgcgat ggtgttccat cagccgtcgg atatcctcgt gcgcgcgtgg gcgcagcgtc tcggcctctc gccggatcaa tacgtgattc gcctggccga ctgcgcgtgc ctcgtgtcgg ctgcggtgcc gctcgcgctc tacgaatcgc ttcgccgcgg cgtcgtgaag ccgggctcgc aagtggtgat cgcgggcgcg ggcgcgggct ggggcttcgg cgcgcagctg tggcgctggg gcgagacggt cgtgaccgac gcggcgccga cggtcgaggc ggcggagagc gaacatgccg cataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa