ButhA.00169.a

Replicative DNA helicase (EC 3.6.1.-) (BTH_I2183) (dnaB)

CENTER ID: ButhA.00169.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00169.a.A1.GE32789 full length 1 460
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.5000
RefSeq: YP_442704.1
UniProt: Q2SWJ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNAPKDPQIE SLKVPPHSIE AEQSVLGGLL LDNGAWDRIA DFLSQSDFYR YDHRVIFEHI GRLIAATRPA DVVTVYEALT TSGKAEDVGG LAYLNALAQN TPSAANIRRY AEIVRDRAVL RRLVSVADEI SADAFNPQGK EVRQLLDEAE SKVFSIAEDG ARGTQGFLEI GPLLTQVVER IDTLYHTANP SDVTGTPTGF VDLDRMTSGM HGGELIIVAG RPSMGKTAFS MNIGEYVAVE YGLPVAVFSM EMPGTQLVMR MLGSVGRLDQ HRMRTGRLTD EDWPKLTHAV QKMSEAQLFI DETGGLNPME LRSRARRLAR QCGKLGLIIV DYLQLMTGSS QGENRATEIS EISRSLKSLA KELDVPVIAL SQLNRGLEQR PNKRPVMSDL RESGAIEQDA DVILFIYRDE VYNPDSPDKG TAEIIIGKQR NGPIGPVRLT FLGQYTKFDN FAGAQNFYGE
NT Sequence
atgaacgcgc cgaaagaccc ccaaatcgaa tcgctgaaag tcccgccgca ctcgatcgag gccgagcagt ccgtgctcgg cggcctgttg ctcgataacg gcgcctggga ccggatcgcc gacttcctgt cgcagagcga tttctaccgg tacgaccatc gcgtcatttt cgagcacatc ggccggctca tcgcggcgac gcgcccggcc gacgtcgtca ccgtctacga ggcgctcacg acgtccggca aggcggagga cgtgggcggg ctcgcgtatc tgaacgcgct cgcgcagaac acgccgagcg ccgcgaacat ccgccgctac gcggaaatcg tccgcgatcg cgcggtattg cgccggctcg tgtcggtcgc cgacgaaatc tcggccgatg cgttcaatcc gcagggcaag gaagtccgcc agctgctcga cgaggccgaa tcgaaggtgt tctcgatcgc cgaggacggc gcgcgcggca cgcagggctt cctcgagatc gggccgctcc tcacgcaagt cgtcgagcgg atcgacaccc tctatcacac cgcgaatccg agcgacgtga caggcacgcc gacgggcttc gtcgacctcg accggatgac gtccggcatg cacggcggcg agctgatcat cgtcgcgggc cgcccgtcga tgggtaagac ggcattctcg atgaacatcg gcgaatacgt cgccgtcgag tacggcttgc ccgtcgcggt gttctcgatg gaaatgccgg gcacgcagct cgtgatgcgg atgctcggct cggtcggcag gctcgatcag caccggatgc gcaccgggcg cctgaccgac gaggattggc cgaagctcac gcacgcggtg cagaagatga gcgaggcgca gctcttcatc gacgagacgg gcggcctgaa tccgatggaa ttgcgctcgc gcgcgcggcg tctcgcgcgc cagtgcggca agctcgggct catcatcgtc gattacctgc agctgatgac gggctcgtcg cagggcgaga accgcgcgac cgaaatctcg gaaatttctc gctcgctgaa gagcctcgcg aaggagctcg acgtgccggt gatcgcgctg tcgcagctca atcgcgggct cgagcagcgg ccgaacaagc ggccggtgat gtcggatctg cgggaatcgg gcgcaatcga acaggatgcg gacgtgatcc tgttcattta ccgtgacgaa gtgtacaacc cggacagccc cgacaagggc accgccgaga tcatcatcgg caagcagcgt aacggcccga tcggccccgt tcggctcacg ttcctggggc aatacacgaa attcgacaat tttgcagggg cgcaaaactt ctacggcgag tag
Details for ButhA.00169.a.A1.GE32789
HARVESTED ON: 6/28/2011
SEQUENCED ON: 7/22/2011
EXPECTED MW: 52kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 95
Validated AA Sequence
MNAPKDPQIE SLKVPPHSIE AEQSVLGGLL LDNGAWDRIA DFLSQSDFYR YDHRVIFEHI GRLIAATRPA DVVTVYEALT TSGKAEDVGG LAYLNALAQN TPSAANIRRY AEIVRDRAVL RRLVSVADEI SADAFNPQGK EVRQLLDEAE SKVFSIAEDG ARGTQGFLEI GPLLTQVVER IDTLYHTANP SDVTGTPTGF VDLDRMTSGM HGGELIIVAG RPSMGKTAFS MNIGEYVAVE YGLPVAVFSM EMPGTQLVMR MLGSVGRLDQ HRMRTXRLTD EDWPKLTHAV QKMSEAQLFX DETGGLNPME LRSRARRLAR QCGKLGLIIV DYLQXMTGSS QGENRATEIS EISRSXKSLA KELDVPVIAL SQLNRGLEQR PNKRPVMSDL RESGAIEQDA DVILFIYRDE VYNPDSPDKG TAEIIIGKQR NGPIGPVRLT FLGQYTKFDN FAGAQNFYGE
Validated NT Sequence
ttattactcg ccgtagaagt tttgcgcccc tgcaaaattg tcgaatttcg tgtattgccc caggaacgtg agccgaacgg ggccgatcgg gccgttacgc tgcttgccga tgatgatctc ggcggtgccc ttgtcggggc tgtccgggtt gtacacttcg tcacggtaaa tgaacaggat cacgtccgca tcctgttcga ttgcgcccga ttcccgcaga tccgacatca ccggccgctt gttcggccgc tgctcgagcc cgcgattgag ctgcgacagc gcgatcaccg gcacgtcgag ctccttcgcg aggctcttca ncgagcgaga aatttccgag atttcggtcg cgcggttctc gccctgcgac gagcccgtca tcanctgcag gtaatcgacg atgatgagcc cgagcttgcc gcactggcgc gcgagacgcc gcgcgcgcga gcgcaattcc atcggattca ggccgcccgt ctcgtcnatg aagagctgcg cctcgctcat cttctgcacc gcgtgcgtga gcttcggcca atcctcgtcg gtcaggcgcc nggtgcgcat ccggtgctga tcgagcctgc cgaccgagcc gagcatccgc atcacgagct gcgtgcccgg catttccatc gagaacaccg cgacgggcaa gccgtactcg acggcgacgt attcgccgat gttcatcgag aatgccgtct tacccatcga cgggcggccc gcgacgatga tcagctcgcc gccgtgcatg ccggacgtca tccggtcgag gtcgacgaag cccgtcggcg tgcctgtcac gtcgctcgga ttcgcggtgt gatagagggt gtcgatccgc tcgacgactt gcgtgaggag cggcccgatc tcgaggaagc cctgcgtgcc gcgcgcgccg tcctcggcga tcgagaacac cttcgattcg gcctcgtcga gcagctggcg gacttccttg ccctgcggat tgaacgcatc ggccgagatt tcgtcggcga ccgacacgag ccggcgcaat accgcgcgat cgcggacgat ttccgcgtag cggcggatgt tcgcggcgct cggcgtgttc tgcgcgagcg cgttcagata cgcgagcccg cccacgtcct ccgccttgcc ggacgtcgtg agcgcctcgt agacggtgac gacgtcggcc gggcgcgtcg ccgcgatgag ccggccgatg tgctcgaaaa tgacgcgatg gtcgtaccgg tagaaatcgc tctgcgacag gaagtcggcg atccggtccc aggcgccgtt atcgagcaac aggccgccga gcacggactg ctcggcctcg atcgagtgcg gcgggacttt cagcgattcg atttgggggt ctttcggcgc gttcatcgaa ccaggaccct gggtctgagc ttccagg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNAPKDPQI ESLKVPPHSI EAEQSVLGGL LLDNGAWDRI ADFLSQSDFY RYDHRVIFEH IGRLIAATRP ADVVTVYEAL TTSGKAEDVG GLAYLNALAQ NTPSAANIRR YAEIVRDRAV LRRLVSVADE ISADAFNPQG KEVRQLLDEA ESKVFSIAED GARGTQGFLE IGPLLTQVVE RIDTLYHTAN PSDVTGTPTG FVDLDRMTSG MHGGELIIVA GRPSMGKTAF SMNIGEYVAV EYGLPVAVFS MEMPGTQLVM RMLGSVGRLD QHRMRTGRLT DEDWPKLTHA VQKMSEAQLF IDETGGLNPM ELRSRARRLA RQCGKLGLII VDYLQLMTGS SQGENRATEI SEISRSLKSL AKELDVPVIA LSQLNRGLEQ RPNKRPVMSD LRESGAIEQD ADVILFIYRD EVYNPDSPDK GTAEIIIGKQ RNGPIGPVRL TFLGQYTKFD NFAGAQNFYG E
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacgcgccg aaagaccccc aaatcgaatc gctgaaagtc ccgccgcact cgatcgaggc cgagcagtcc gtgctcggcg gcctgttgct cgataacggc gcctgggacc ggatcgccga cttcctgtcg cagagcgatt tctaccggta cgaccatcgc gtcattttcg agcacatcgg ccggctcatc gcggcgacgc gcccggccga cgtcgtcacc gtctacgagg cgctcacgac gtccggcaag gcggaggacg tgggcgggct cgcgtatctg aacgcgctcg cgcagaacac gccgagcgcc gcgaacatcc gccgctacgc ggaaatcgtc cgcgatcgcg cggtattgcg ccggctcgtg tcggtcgccg acgaaatctc ggccgatgcg ttcaatccgc agggcaagga agtccgccag ctgctcgacg aggccgaatc gaaggtgttc tcgatcgccg aggacggcgc gcgcggcacg cagggcttcc tcgagatcgg gccgctcctc acgcaagtcg tcgagcggat cgacaccctc tatcacaccg cgaatccgag cgacgtgaca ggcacgccga cgggcttcgt cgacctcgac cggatgacgt ccggcatgca cggcggcgag ctgatcatcg tcgcgggccg cccgtcgatg ggtaagacgg cattctcgat gaacatcggc gaatacgtcg ccgtcgagta cggcttgccc gtcgcggtgt tctcgatgga aatgccgggc acgcagctcg tgatgcggat gctcggctcg gtcggcaggc tcgatcagca ccggatgcgc accgggcgcc tgaccgacga ggattggccg aagctcacgc acgcggtgca gaagatgagc gaggcgcagc tcttcatcga cgagacgggc ggcctgaatc cgatggaatt gcgctcgcgc gcgcggcgtc tcgcgcgcca gtgcggcaag ctcgggctca tcatcgtcga ttacctgcag ctgatgacgg gctcgtcgca gggcgagaac cgcgcgaccg aaatctcgga aatttctcgc tcgctgaaga gcctcgcgaa ggagctcgac gtgccggtga tcgcgctgtc gcagctcaat cgcgggctcg agcagcggcc gaacaagcgg ccggtgatgt cggatctgcg ggaatcgggc gcaatcgaac aggatgcgga cgtgatcctg ttcatttacc gtgacgaagt gtacaacccg gacagccccg acaagggcac cgccgagatc atcatcggca agcagcgtaa cggcccgatc ggccccgttc ggctcacgtt cctggggcaa tacacgaaat tcgacaattt tgcaggggcg caaaacttct acggcgagta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a