ButhA.00168.a
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (EC 2.7.7.60) (4-diphosphocytidyl-2C-methyl-D-erythritol synthase) (MEP cytidylyltransferase) (MCT) (BTH_I2089) (ispD)
| CENTER ID: | ButhA.00168.a |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00168.a.A1.GE32734 | full length | 1 | 236 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00168.a.A1.PW37637 | full length | 1 | 236 |
Structures
4YS8
DEPOSITED: 3/16/2015
DETERMINATION: XRay
CLONE: ButhA.00168.a.A1.GE32734
PROTEIN: ButhA.00168.a.A1.PW33338
4ZDQ
DEPOSITED: 4/18/2015
DETERMINATION: XRay
CLONE: ButhA.00168.a.A1.GE32734
PROTEIN: ButhA.00168.a.A1.PW33338
Publications by SSGCID
Crystal structure and biophysical characterization of IspD from Burkholderia thailandensis and Mycobacterium paratuberculosis
Blain JM, Dranow DM, Edwards TE, Hagen TJ, Horn JR, Lorimer DD, Myers J, Myler PJ, Pierce PG, Sullivan A, Weiss MS, Higgins T, Hartnett B
Acta Crystallogr F Struct Biol Commun - 2024
volume 80 Pt 2, issue 2024 Feb 1, pages 43-51
PMID: 38305785; PMCID: PMC10836425
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_129829 |
| PATRIC ID: | fig|271848.6.peg.4901 |
| RefSeq: | YP_442613.1 |
| UniProt: | Q2SWT6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTSRLFALIP CAGTGSRSGS ALPKQYRTLA GRALLHYTLA AFDACSEFAQ TLVVISPDDA HFDARRFAGL RFAVRRCGGA SRQASVMNGL IQLAEFGATD ADWVLVHDAA RPGITPALIR TLIGALKDDP VGGIVALPVA DTLKRVPAGG DAIERTESRN GLWQAQTPQM FRIGMLRDAI QRAQLEGRDL TDEASAIEWA GHTPRVVQGS LRNFKVTYPE DFDLAEAILA HPARAS
NT Sequence
gtgacctccc gactcttcgc cctgattccg tgcgccggca ccggcagccg ctccggctcg gcattgccga agcaataccg cacgctcgcc ggccgcgcgc tcctgcatta cacgctcgcc gcgttcgacg cgtgcagcga gttcgcgcaa acgctcgtcg tgatttcccc cgacgacgcc cacttcgacg cccgccgctt tgccggtctg cgcttcgcgg tgcggcgctg cggcggcgcg tcccgacagg cgtcggtgat gaacgggctg atccagctcg ccgaattcgg cgcgacggac gccgactggg tgctcgtgca cgacgccgcg cgccccggca tcacgccggc gctgatccgc acgctgatcg gcgcgctgaa ggacgacccc gtcggcggca tcgtcgcgct gccggtcgcc gatacgctca agcgcgtgcc ggcgggcggc gacgcgatcg agcgcaccga atcgcgcaac ggcctctggc aggcgcagac gccgcagatg ttccgcatcg gcatgctgcg cgacgcgatc cagcgcgcgc agctcgaagg tcgcgatctg accgacgaag cgagcgcgat cgaatgggcg ggccatacgc cgcgcgtcgt gcagggcagt ctgcgcaact tcaaggtcac gtacccggag gatttcgacc tcgcggaggc gattctcgcg catccggcgc gcgccagttg a
Details for ButhA.00168.a.A1.GE32734
| HARVESTED ON: | 6/20/2011 |
| SEQUENCED ON: | 5/1/2015 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTSRLFAL IPCAGTGSRS GSALPKQYRT LAGRALLHYT LAAFDACSEF AQTLVVISPD DAHFDARRFA GLRFAVRRCG GASRQASVMN GLIQLAEFGA TDADWVLVHD AARPGITPAL IRTLIGALKD DPVGGIVALP VADTLKRVPA GGDAIERTES RNGLWQAQTP QMFRIGMLRD AIQRAQLEGR DLTDEASAIE WAGHTPRVVQ GSLRNFKVTY PEDFDLAEAI LAHPARAS
Validated NT Sequence
ttattaactg gcgcgcgccg gatgcgcgag aatcgcctcc gcgaggtcga aatcctccgg gtacgtgacc ttgaagttgc gcagactgcc ctgcacgacg cgcggcgtat ggcccgccca ttcgatcgcg ctcgcttcgt cggtcagatc gcgaccttcg agctgcgcgc gctggatcgc gtcgcgcagc atgccgatgc ggaacatctg cggcgtctgc gcctgccaga ggccgttgcg cgattcggtg cgctcgatcg cgtcgccgcc cgccggcacg cgcttgagcg tatcggcgac cggcagcgcg acgatgccgc cgacggggtc gtccttcagc gcgccgatca gcgtgcggat cagcgccggc gtgatgccgg ggcgcgcggc gtcgtgcacg agcacccagt cggcgtccgt cgcgccgaat tcggcgagct ggatcagccc gttcatcacc gacgcctgtc gggacgcgcc gccgcagcgc cgcaccgcga agcgcagacc ggcaaagcgg cgggcgtcga agtgggcgtc gtcgggggaa atcacgacga gcgtttgcgc gaactcgctg cacgcgtcga acgcggcgag cgtgtaatgc aggagcgcgc ggccggcgag cgtgcggtat tgcttcggca atgccgagcc ggagcggctg ccggtgccgg cgcacggaat cagggcgaag agtcgggagg tcaccatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTSRLFAL IPCAGTGSRS GSALPKQYRT LAGRALLHYT LAAFDACSEF AQTLVVISPD DAHFDARRFA GLRFAVRRCG GASRQASVMN GLIQLAEFGA TDADWVLVHD AARPGITPAL IRTLIGALKD DPVGGIVALP VADTLKRVPA GGDAIERTES RNGLWQAQTP QMFRIGMLRD AIQRAQLEGR DLTDEASAIE WAGHTPRVVQ GSLRNFKVTY PEDFDLAEAI LAHPARAS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacctcc cgactcttcg ccctgattcc gtgcgccggc accggcagcc gctccggctc ggcattgccg aagcaatacc gcacgctcgc cggccgcgcg ctcctgcatt acacgctcgc cgcgttcgac gcgtgcagcg agttcgcgca aacgctcgtc gtgatttccc ccgacgacgc ccacttcgac gcccgccgct ttgccggtct gcgcttcgcg gtgcggcgct gcggcggcgc gtcccgacag gcgtcggtga tgaacgggct gatccagctc gccgaattcg gcgcgacgga cgccgactgg gtgctcgtgc acgacgccgc gcgccccggc atcacgccgg cgctgatccg cacgctgatc ggcgcgctga aggacgaccc cgtcggcggc atcgtcgcgc tgccggtcgc cgatacgctc aagcgcgtgc cggcgggcgg cgacgcgatc gagcgcaccg aatcgcgcaa cggcctctgg caggcgcaga cgccgcagat gttccgcatc ggcatgctgc gcgacgcgat ccagcgcgcg cagctcgaag gtcgcgatct gaccgacgaa gcgagcgcga tcgaatgggc gggccatacg ccgcgcgtcg tgcagggcag tctgcgcaac ttcaaggtca cgtacccgga ggatttcgac ctcgcggagg cgattctcgc gcatccggcg cgcgccagtt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for ButhA.00168.a.A1.PW37637
| PURIFICATION DATe: | 1/26/2015 |
| CONCENTRATION: | 2.8mg/ml |
| OBSERVED MW: | 27kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 30000µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTSRLFAL IPCAGTGSRS GSALPKQYRT LAGRALLHYT LAAFDACSEF AQTLVVISPD DAHFDARRFA GLRFAVRRCG GASRQASVMN GLIQLAEFGA TDADWVLVHD AARPGITPAL IRTLIGALKD DPVGGIVALP VADTLKRVPA GGDAIERTES RNGLWQAQTP QMFRIGMLRD AIQRAQLEGR DLTDEASAIE WAGHTPRVVQ GSLRNFKVTY PEDFDLAEAI LAHPARAS
Validated NT Sequence
ttattaactg gcgcgcgccg gatgcgcgag aatcgcctcc gcgaggtcga aatcctccgg gtacgtgacc ttgaagttgc gcagactgcc ctgcacgacg cgcggcgtat ggcccgccca ttcgatcgcg ctcgcttcgt cggtcagatc gcgaccttcg agctgcgcgc gctggatcgc gtcgcgcagc atgccgatgc ggaacatctg cggcgtctgc gcctgccaga ggccgttgcg cgattcggtg cgctcgatcg cgtcgccgcc cgccggcacg cgcttgagcg tatcggcgac cggcagcgcg acgatgccgc cgacggggtc gtccttcagc gcgccgatca gcgtgcggat cagcgccggc gtgatgccgg ggcgcgcggc gtcgtgcacg agcacccagt cggcgtccgt cgcgccgaat tcggcgagct ggatcagccc gttcatcacc gacgcctgtc gggacgcgcc gccgcagcgc cgcaccgcga agcgcagacc ggcaaagcgg cgggcgtcga agtgggcgtc gtcgggggaa atcacgacga gcgtttgcgc gaactcgctg cacgcgtcga acgcggcgag cgtgtaatgc aggagcgcgc ggccggcgag cgtgcggtat tgcttcggca atgccgagcc ggagcggctg ccggtgccgg cgcacggaat cagggcgaag agtcgggagg tcaccatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTSRLFAL IPCAGTGSRS GSALPKQYRT LAGRALLHYT LAAFDACSEF AQTLVVISPD DAHFDARRFA GLRFAVRRCG GASRQASVMN GLIQLAEFGA TDADWVLVHD AARPGITPAL IRTLIGALKD DPVGGIVALP VADTLKRVPA GGDAIERTES RNGLWQAQTP QMFRIGMLRD AIQRAQLEGR DLTDEASAIE WAGHTPRVVQ GSLRNFKVTY PEDFDLAEAI LAHPARAS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACCTCC CGACTCTTCG CCCTGATTCC GTGCGCCGGC ACCGGCAGCC GCTCCGGCTC GGCATTGCCG AAGCAATACC GCACGCTCGC CGGCCGCGCG CTCCTGCATT ACACGCTCGC CGCGTTCGAC GCGTGCAGCG AGTTCGCGCA AACGCTCGTC GTGATTTCCC CCGACGACGC CCACTTCGAC GCCCGCCGCT TTGCCGGTCT GCGCTTCGCG GTGCGGCGCT GCGGCGGCGC GTCCCGACAG GCGTCGGTGA TGAACGGGCT GATCCAGCTC GCCGAATTCG GCGCGACGGA CGCCGACTGG GTGCTCGTGC ACGACGCCGC GCGCCCCGGC ATCACGCCGG CGCTGATCCG CACGCTGATC GGCGCGCTGA AGGACGACCC CGTCGGCGGC ATCGTCGCGC TGCCGGTCGC CGATACGCTC AAGCGCGTGC CGGCGGGCGG CGACGCGATC GAGCGCACCG AATCGCGCAA CGGCCTCTGG CAGGCGCAGA CGCCGCAGAT GTTCCGCATC GGCATGCTGC GCGACGCGAT CCAGCGCGCG CAGCTCGAAG GTCGCGATCT GACCGACGAA GCGAGCGCGA TCGAATGGGC GGGCCATACG CCGCGCGTCG TGCAGGGCAG TCTGCGCAAC TTCAAGGTCA CGTACCCGGA GGATTTCGAC CTCGCGGAGG CGATTCTCGC GCATCCGGCG CGCGCCAGTT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA