SSGCID: Target Page

ButhA.00166.b

UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (EC 3.5.1.-) (UDP-3-O-acyl-GlcNAc deacetylase) (BTH_I1125) (lpxC)

CENTER ID:	ButhA.00166.b
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00166.b.A1.GE32788	full length		1	305

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00166.b.A1.PS01257	full length		1	305

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.3881
RefSeq:	YP_441673.1
UniProt:	Q2SZH6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLKQRTIKSI VKTVGIGLHS GRKVELTLRP AAPDTGIVFS RVDLPTPVDI PASALSIGDT RLASVLQKDG ARVSTVEHLM SACAGLGIDN LYVDVTAEEI PIMDGSAASF VFLIQSAGIE EQNAAKKFIK VTKPVEIRDG DKFARLDPYF GFKLKFTIDF RHPAVDKTGQ ELEVDFANTS YVREIARART FGFAHEVEMM RELGLARGGS MDNAIVLDEY RILNNDGLRY DDEFVKHKML DAIGDLYVVG HPLLASYTAY KSGHGLNNAL LRELLAHEDA YEIVTFDDPK AAPTGFGFDA QTAFA

NT Sequence

atgttgaagc agcgcaccat caaatcgatc gtcaagactg tcggcatcgg cctgcactcg ggccgcaagg tcgaactgac gcttcgcccc gcggcgccgg acacgggcat cgtgttctcg cgcgtcgatc tgccgacgcc cgtcgacatt ccggcttcgg cgctgtcgat cggcgacacg cggctcgcgt ccgtgctgca gaaggacggc gcgcgcgtgt cgacggtcga gcatctgatg tcggcgtgtg cgggcctcgg catcgacaac ctctacgtcg acgtgacggc cgaggaaatc ccgatcatgg acggcagtgc ggcgtcgttc gtgttcctga tccagtcggc cggcatcgaa gagcagaacg cggcgaagaa gttcatcaag gtgacgaagc ccgtcgagat tcgcgacggc gacaagttcg cgcgtctcga tccctacttc ggcttcaagc tgaagttcac gatcgatttc cgccatccgg ccgtcgacaa gacgggccag gaactcgagg ttgacttcgc gaacacgtcg tacgtgcgcg agatcgcgcg cgcgcgcaca ttcggcttcg cgcacgaggt cgagatgatg cgcgagctcg ggctggcgcg cggcggcagc atggacaacg cgatcgtgct cgacgaatac cggattctca ataacgacgg actccgctac gacgacgagt tcgtcaagca caagatgctc gacgcgatcg gcgatctgta cgtggtcggc catccgctcc tcgcgtcgta caccgcgtac aagtcgggcc acgggctgaa caatgcgctg ctgcgcgagc tgctcgcgca cgaggacgcc tacgagatcg tgacgttcga cgatccgaag gcggcgccga cgggcttcgg cttcgacgcg cagacggcat tcgcgtga

Details for ButhA.00166.b.A1.GE32788

HARVESTED ON:	6/28/2011
SEQUENCED ON:	7/22/2011
EXPECTED MW:	35kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMLKQRTI KSIVKTVGIG LHSGRKVELT LRPAAPDTGI VFSRVDLPTP VDIPASALSI GDTRLASVLQ KDGARVSTVE HLMSACAGLG IDNLYVDVTA EEIPIMDGSA ASFVFLIQSA GIEEQNAAKK FIKVTKPVEI RDGDKFARLD PYFGFKLKFT IDFRHPAVDK TGQELEVDFA NTSYVREIAR ARTFGFAHEV EMMRELGLAR GGSMDNAIVL DEYRILNNDG LRYDDEFVKH KMLDAIGDLY VVGHPLLASY TAYKSGHGLN NALLRELLAH EDAYEIVTFD DPKAAPTGFG FDAQTAFA

Validated NT Sequence

ttattacgcg aatgccgtct gcgcgtcgaa gccgaagccc gtcggcgccg ccttcggatc gtcgaacgtc acgatctcgt aggcgtcctc gtgcgcgagc agctcgcgca gcagcgcatt gttcagcccg tggcccgact tgtacgcggt gtacgacgcg aggagcggat ggccgaccac gtacagatcg ccgatcgcgt cgagcatctt gtgcttgacg aactcgtcgt cgtagcggag tccgtcgtta ttgagaatcc ggtattcgtc gagcacgatc gcgttgtcca tgctgccgcc gcgcgccagc ccgagctcgc gcatcatctc gacctcgtgc gcgaagccga atgtgcgcgc gcgcgcgatc tcgcgcacgt acgacgtgtt cgcgaagtca acctcgagtt cctggcccgt cttgtcgacg gccggatggc ggaaatcgat cgtgaacttc agcttgaagc cgaagtaggg atcgagacgc gcgaacttgt cgccgtcgcg aatctcgacg ggcttcgtca ccttgatgaa cttcttcgcc gcgttctgct cttcgatgcc ggccgactgg atcaggaaca cgaacgacgc cgcactgccg tccatgatcg ggatttcctc ggccgtcacg tcgacgtaga ggttgtcgat gccgaggccc gcacacgccg acatcagatg ctcgaccgtc gacacgcgcg cgccgtcctt ctgcagcacg gacgcgagcc gcgtgtcgcc gatcgacagc gccgaagccg gaatgtcgac gggcgtcggc agatcgacgc gcgagaacac gatgcccgtg tccggcgccg cggggcgaag cgtcagttcg accttgcggc ccgagtgcag gccgatgccg acagtcttga cgatcgattt gatggtgcgc tgcttcaaca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatgg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLKQRTIKS IVKTVGIGLH SGRKVELTLR PAAPDTGIVF SRVDLPTPVD IPASALSIGD TRLASVLQKD GARVSTVEHL MSACAGLGID NLYVDVTAEE IPIMDGSAAS FVFLIQSAGI EEQNAAKKFI KVTKPVEIRD GDKFARLDPY FGFKLKFTID FRHPAVDKTG QELEVDFANT SYVREIARAR TFGFAHEVEM MRELGLARGG SMDNAIVLDE YRILNNDGLR YDDEFVKHKM LDAIGDLYVV GHPLLASYTA YKSGHGLNNA LLRELLAHED AYEIVTFDDP KAAPTGFGFD AQTAFA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgaagcag cgcaccatca aatcgatcgt caagactgtc ggcatcggcc tgcactcggg ccgcaaggtc gaactgacgc ttcgccccgc ggcgccggac acgggcatcg tgttctcgcg cgtcgatctg ccgacgcccg tcgacattcc ggcttcggcg ctgtcgatcg gcgacacgcg gctcgcgtcc gtgctgcaga aggacggcgc gcgcgtgtcg acggtcgagc atctgatgtc ggcgtgtgcg ggcctcggca tcgacaacct ctacgtcgac gtgacggccg aggaaatccc gatcatggac ggcagtgcgg cgtcgttcgt gttcctgatc cagtcggccg gcatcgaaga gcagaacgcg gcgaagaagt tcatcaaggt gacgaagccc gtcgagattc gcgacggcga caagttcgcg cgtctcgatc cctacttcgg cttcaagctg aagttcacga tcgatttccg ccatccggcc gtcgacaaga cgggccagga actcgaggtt gacttcgcga acacgtcgta cgtgcgcgag atcgcgcgcg cgcgcacatt cggcttcgcg cacgaggtcg agatgatgcg cgagctcggg ctggcgcgcg gcggcagcat ggacaacgcg atcgtgctcg acgaataccg gattctcaat aacgacggac tccgctacga cgacgagttc gtcaagcaca agatgctcga cgcgatcggc gatctgtacg tggtcggcca tccgctcctc gcgtcgtaca ccgcgtacaa gtcgggccac gggctgaaca atgcgctgct gcgcgagctg ctcgcgcacg aggacgccta cgagatcgtg acgttcgacg atccgaaggc ggcgccgacg ggcttcggct tcgacgcgca gacggcattc gcgtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for ButhA.00166.b.A1.PS01257

PURIFICATION DATe:	11/2/2011
CONCENTRATION:	38.49mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	50µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGAAGCAG CGCACCATCA AATCGATCGT CAAGACTGTC GGCATCGGCC TGCACTCGGG CCGCAAGGTC GAACTGACGC TTCGCCCCGC GGCGCCGGAC ACGGGCATCG TGTTCTCGCG CGTCGATCTG CCGACGCCCG TCGACATTCC GGCTTCGGCG CTGTCGATCG GCGACACGCG GCTCGCGTCC GTGCTGCAGA AGGACGGCGC GCGCGTGTCG ACGGTCGAGC ATCTGATGTC GGCGTGTGCG GGCCTCGGCA TCGACAACCT CTACGTCGAC GTGACGGCCG AGGAAATCCC GATCATGGAC GGCAGTGCGG CGTCGTTCGT GTTCCTGATC CAGTCGGCCG GCATCGAAGA GCAGAACGCG GCGAAGAAGT TCATCAAGGT GACGAAGCCC GTCGAGATTC GCGACGGCGA CAAGTTCGCG CGTCTCGATC CCTACTTCGG CTTCAAGCTG AAGTTCACGA TCGATTTCCG CCATCCGGCC GTCGACAAGA CGGGCCAGGA ACTCGAGGTT GACTTCGCGA ACACGTCGTA CGTGCGCGAG ATCGCGCGCG CGCGCACATT CGGCTTCGCG CACGAGGTCG AGATGATGCG CGAGCTCGGG CTGGCGCGCG GCGGCAGCAT GGACAACGCG ATCGTGCTCG ACGAATACCG GATTCTCAAT AACGACGGAC TCCGCTACGA CGACGAGTTC GTCAAGCACA AGATGCTCGA CGCGATCGGC GATCTGTACG TGGTCGGCCA TCCGCTCCTC GCGTCGTACA CCGCGTACAA GTCGGGCCAC GGGCTGAACA ATGCGCTGCT GCGCGAGCTG CTCGCGCACG AGGACGCCTA CGAGATCGTG ACGTTCGACG ATCCGAAGGC GGCGCCGACG GGCTTCGGCT TCGACGCGCA GACGGCATTC GCGTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA