ButhA.00162.a

DNA gyrase subunit B (EC 5.99.1.3) (BTH_I3241) (gyrB)

CENTER ID: ButhA.00162.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00162.a.A1.GE32732 full length 1 867
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.6132
RefSeq: YP_443735.2
UniProt: Q2STL4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAIRTLERRR ATPAPERGEK PEAARFFRRK HPAATRVAQQ NRKTPMTEQH NSQPENSYGA SSIQILEGLE AVRKRPGMYI GDTSDGTGLH HLVFEVLDNS IDEALAGYCN DIHVTIHADN SISVTDNGRG IPTDVKLNDK HEPKRSAAEI VMTELHAGGK FDQNSYKVSG GLHGVGVSCV NALSSWLRLT VRRDGKKHFM EFHRGIAQNR VLEERDGEQV SPMQVIGDTE NRGTEVHFMA DSTIFGTVEY HYDILAKRIR ELSFLNNGVR IRLTDQRSGK EDDFAFAGGV KGFVEYINKT KSVLHPTIFH INGEKDGVGV EVAMQWNDSY NENVLCFTNN IPQRDGGTHL TGLRAAMTRV INKYIADNEI AKKAKVETSG DDMREGLSCV LSVKVPEPKF SSQTKDKLVS SEVRAPVEEV VAKALEEFLL ETPNDAKIIC GKIVEAARAR DAARKAREMT RRKGVLDGVG LPGKLADCQE KDPAKSEIYI VEGDSAGGSA KQGRDRKFQA ILPLRGKVLN VEKARYDKLL SSEQIVTLVT ALGCGIGKDD YNLDKLRYHR IIIMTDADVD GAHIRTLLLT FFYRQMPEMI ERGYVYIAQP PLYKVKAGRD ERYLKDDTEL NAHMLRLALQ GSELVPTENG TPISGDALGE LARSYLLARG VVERLSRLYD PAALEAVMDG VAIDLSSEAS TEASAKALAA MLSDDSKTEV RVVPAYDAVR EQRSLRVERT HHGNVRVSVI DEEFQHTADY QQLVNTANTF KGLIGAGAAI KRGERSSNVA DFKEAMKWLM ADAERNMSKQ RYKGLGEMNP EQLWETTMDP AVRRLLRVQI EDAIAADGIF TTLMGDEVEP RRAFIENNAL RAGNIDV
NT Sequence
atggcgattc gcacgcttga gcgccggcgc gcgacgccgg cccccgaaag gggcgaaaaa cccgaggcgg cccggttttt tcggcggaag caccccgccg ccactcgagt agcccagcag aaccggaaga cacccatgac tgaacagcac aattcgcagc ccgaaaatag ctacggcgcg tcgtcgatcc agatcctcga aggcctggaa gcggtgcgca agcgacccgg gatgtacatc ggcgacacgt cggacggcac cggtctgcac cacctcgtgt tcgaggtgct cgacaactcg atcgacgaag cgcttgccgg ctactgcaac gacatccacg tgaccattca cgcggacaat tcgatctccg tgaccgacaa cggccgcggc atcccgaccg acgtcaagct caacgacaag cacgaaccga agcgctcggc cgccgagatc gtgatgaccg agctgcacgc gggcggcaag ttcgaccaga acagctacaa ggtgtcgggc ggcctgcacg gcgtcggcgt atcgtgcgtg aacgcgctct ccagctggct gcgcctcacc gtgcgccgcg acggcaagaa gcacttcatg gaattccatc gcggcatcgc gcagaaccgc gtgctcgagg agcgcgacgg cgagcaggtg tcgccgatgc aggtgatcgg cgacaccgag aaccgcggca ccgaagtgca cttcatggcc gattcgacga tcttcggcac cgtcgaatac cactacgaca tcctcgcgaa acgcatccgc gagctgtcgt tcctgaacaa cggcgtgcgc atccgcctga ccgaccagcg ctccggcaag gaagacgatt tcgcgttcgc gggcggcgtg aagggcttcg tcgagtacat caacaagacg aagagcgtgc tgcatccgac gatcttccac atcaacggcg agaaggacgg cgtcggcgtg gaagtggcga tgcagtggaa tgacagctac aacgaaaacg tgctgtgctt cacgaacaac atcccgcagc gcgacggcgg cacgcacttg accggcctgc gcgcggcgat gacgcgcgtg atcaacaagt acatcgccga caacgaaatc gcgaagaagg caaaggtcga gacgagcggc gacgacatgc gcgaagggct ttcgtgcgtg ctgtcggtga aggtgccgga gccgaagttc agctcgcaga cgaaggacaa gctggtttcg tccgaagtgc gcgcgccggt ggaggaagtg gtggcgaagg cgctcgagga gttcctgctc gaaacgccga acgacgcgaa gatcatctgc ggcaagatcg tcgaagcggc gcgcgcgcgc gatgccgcgc gcaaggcgcg cgagatgacg cgccgcaagg gcgtgctcga cggcgtgggc ctgccgggca agctcgcgga ttgccaggag aaggacccgg cgaaatcgga gatctacatc gtcgagggcg attcggcggg cggctcggcg aagcaagggc gcgaccgcaa gttccaggcg atcctgccgc tgcgcggcaa ggtgctgaac gtggagaagg cgcgctacga caagctgctg tcgtccgagc agatcgtcac gctcgtgacc gcgctcggct gcgggatcgg caaggacgac tacaacctcg acaagctgcg ctaccaccgg atcatcatca tgaccgatgc ggacgtggac ggcgcgcaca tccgcacgct gctgctgacg ttcttctacc ggcagatgcc ggagatgatc gagcgcggct acgtgtacat cgcgcagccg ccgctctaca aggtgaaggc tggccgcgac gagcgctacc tgaaggacga caccgagctg aacgcgcaca tgctgcgcct cgcgctgcag ggctcggagc tcgtgccgac cgagaacggc acaccgatct cgggcgacgc gctcggcgag cttgcgcgct cgtatctgct cgcgcggggc gtggtggagc ggctgagccg cctgtacgac ccggccgcgc tcgaggcggt catggacggc gtcgcgatcg atctgtcgag cgaggcgtcg acggaggcgt cggcgaaggc gctggccgcc atgctgtccg acgattcgaa gaccgaagtg cgcgtcgtgc ccgcttacga tgcggtgcgc gagcagcgct cgctgcgtgt cgagcgcacg catcacggca acgtgcgcgt atcggtgatc gacgaggagt tccagcacac ggctgattat cagcaactcg taaacaccgc gaatacgttc aagggtttga tcggcgcggg ggcggcgatc aagcgcggcg aacggagctc gaacgtggcg gacttcaagg aagcgatgaa gtggctgatg gccgacgccg agcggaacat gtctaagcag cggtataagg ggttgggcga gatgaacccc gagcagctgt gggaaacgac gatggacccg gcggtgcggc ggttgctgcg cgtgcagatc gaggatgcga ttgcggcgga tgggatcttt accaccctca tgggggacga ggtcgagccg cgacgggcgt ttatcgagaa taatgcgttg cgggcgggga atattgatgt ttga
Details for ButhA.00162.a.A1.GE32732
HARVESTED ON: 6/20/2011
SEQUENCED ON: 7/14/2011
EXPECTED MW: 98kDa
OBSERVED MW: 98kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 79
PERCENT COVERAGE: 32
Validated AA Sequence
MGTLEAQTQG PGSMAIRTLE RRRATPAPER GEKPEAARFF RRKHPAATRV AQQNRKTPMT EQHNSQPENS YGASSIQILE GLEAVRKRPG MYIGDTSDGT GLHHLVFEVL DNSIDEALAG YCNDIHVTIH ADNSISVTDN GRGIPTDVXL NDKHEPKRSA AEIVMTELHA GGKFDQNSYK VSGGLHGVXV SCVNALSSWL RLTXXXDGXK HFMEFHXXXA XNRVLEERDG EQVSPMXXSA XXXXAAPXXT XWPXXRXSAP XXTXTTSSRX XSASCXXXXX XXXXXDXXRS GK
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggcgat tcgcacgctt gagcgccggc gcgcgacgcc ggcccccgaa aggggcgaaa aacccgaggc ggcccggttt tttcggcgga agcaccccgc cgccactcga gtagcccagc agaaccggaa gacacccatg actgaacagc acaattcgca gcccgaaaat agctacggcg cgtcgtcgat ccagatcctc gaaggcctgg aagcggtgcg caagcgaccc gggatgtaca tcggcgacac gtcggacggc accggtctgc accacctcgt gttcgaggtg ctcgacaact cgatcgacga agcgcttgcc ggctactgca acgacatcca cgtgaccatt cacgcggaca attcgatctc cgtgaccgac aacggccgcg gcatcccgac cgacgtcnag ctcaacgaca agcacgaacc gaagcgctcg gccgccgaga tcgtgatgac cgagctgcac gcgggcggca agttcgacca gaacagctac aaggtgtcgg gcggnctgca cggcgtnngc gtatcgtgcg tgaacgcgct ctccagctgg ctgcgcctca ccgngnnnng cgacggcnan aagcacttca tggaattcca tcncnncntc gcgcnnaacc gcgtgctcga ggagcgcgac ggcgagcagg tgtcgccgat gnnnngatcg gcgannncna nnnncgcggc accnnnnngc acttnntggc cgatncnacg ancntcggca ccgtnnnnta ccantacgac atcctcgcgn annncatccg cgagctgtcn ntnnnganaa nngnnnnngn ntcnnnctga cnnncnncgn tccggnaagg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAIRTLERR RATPAPERGE KPEAARFFRR KHPAATRVAQ QNRKTPMTEQ HNSQPENSYG ASSIQILEGL EAVRKRPGMY IGDTSDGTGL HHLVFEVLDN SIDEALAGYC NDIHVTIHAD NSISVTDNGR GIPTDVKLND KHEPKRSAAE IVMTELHAGG KFDQNSYKVS GGLHGVGVSC VNALSSWLRL TVRRDGKKHF MEFHRGIAQN RVLEERDGEQ VSPMQVIGDT ENRGTEVHFM ADSTIFGTVE YHYDILAKRI RELSFLNNGV RIRLTDQRSG KEDDFAFAGG VKGFVEYINK TKSVLHPTIF HINGEKDGVG VEVAMQWNDS YNENVLCFTN NIPQRDGGTH LTGLRAAMTR VINKYIADNE IAKKAKVETS GDDMREGLSC VLSVKVPEPK FSSQTKDKLV SSEVRAPVEE VVAKALEEFL LETPNDAKII CGKIVEAARA RDAARKAREM TRRKGVLDGV GLPGKLADCQ EKDPAKSEIY IVEGDSAGGS AKQGRDRKFQ AILPLRGKVL NVEKARYDKL LSSEQIVTLV TALGCGIGKD DYNLDKLRYH RIIIMTDADV DGAHIRTLLL TFFYRQMPEM IERGYVYIAQ PPLYKVKAGR DERYLKDDTE LNAHMLRLAL QGSELVPTEN GTPISGDALG ELARSYLLAR GVVERLSRLY DPAALEAVMD GVAIDLSSEA STEASAKALA AMLSDDSKTE VRVVPAYDAV REQRSLRVER THHGNVRVSV IDEEFQHTAD YQQLVNTANT FKGLIGAGAA IKRGERSSNV ADFKEAMKWL MADAERNMSK QRYKGLGEMN PEQLWETTMD PAVRRLLRVQ IEDAIAADGI FTTLMGDEVE PRRAFIENNA LRAGNIDV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgattcgc acgcttgagc gccggcgcgc gacgccggcc cccgaaaggg gcgaaaaacc cgaggcggcc cggttttttc ggcggaagca ccccgccgcc actcgagtag cccagcagaa ccggaagaca cccatgactg aacagcacaa ttcgcagccc gaaaatagct acggcgcgtc gtcgatccag atcctcgaag gcctggaagc ggtgcgcaag cgacccggga tgtacatcgg cgacacgtcg gacggcaccg gtctgcacca cctcgtgttc gaggtgctcg acaactcgat cgacgaagcg cttgccggct actgcaacga catccacgtg accattcacg cggacaattc gatctccgtg accgacaacg gccgcggcat cccgaccgac gtcaagctca acgacaagca cgaaccgaag cgctcggccg ccgagatcgt gatgaccgag ctgcacgcgg gcggcaagtt cgaccagaac agctacaagg tgtcgggcgg cctgcacggc gtcggcgtat cgtgcgtgaa cgcgctctcc agctggctgc gcctcaccgt gcgccgcgac ggcaagaagc acttcatgga attccatcgc ggcatcgcgc agaaccgcgt gctcgaggag cgcgacggcg agcaggtgtc gccgatgcag gtgatcggcg acaccgagaa ccgcggcacc gaagtgcact tcatggccga ttcgacgatc ttcggcaccg tcgaatacca ctacgacatc ctcgcgaaac gcatccgcga gctgtcgttc ctgaacaacg gcgtgcgcat ccgcctgacc gaccagcgct ccggcaagga agacgatttc gcgttcgcgg gcggcgtgaa gggcttcgtc gagtacatca acaagacgaa gagcgtgctg catccgacga tcttccacat caacggcgag aaggacggcg tcggcgtgga agtggcgatg cagtggaatg acagctacaa cgaaaacgtg ctgtgcttca cgaacaacat cccgcagcgc gacggcggca cgcacttgac cggcctgcgc gcggcgatga cgcgcgtgat caacaagtac atcgccgaca acgaaatcgc gaagaaggca aaggtcgaga cgagcggcga cgacatgcgc gaagggcttt cgtgcgtgct gtcggtgaag gtgccggagc cgaagttcag ctcgcagacg aaggacaagc tggtttcgtc cgaagtgcgc gcgccggtgg aggaagtggt ggcgaaggcg ctcgaggagt tcctgctcga aacgccgaac gacgcgaaga tcatctgcgg caagatcgtc gaagcggcgc gcgcgcgcga tgccgcgcgc aaggcgcgcg agatgacgcg ccgcaagggc gtgctcgacg gcgtgggcct gccgggcaag ctcgcggatt gccaggagaa ggacccggcg aaatcggaga tctacatcgt cgagggcgat tcggcgggcg gctcggcgaa gcaagggcgc gaccgcaagt tccaggcgat cctgccgctg cgcggcaagg tgctgaacgt ggagaaggcg cgctacgaca agctgctgtc gtccgagcag atcgtcacgc tcgtgaccgc gctcggctgc gggatcggca aggacgacta caacctcgac aagctgcgct accaccggat catcatcatg accgatgcgg acgtggacgg cgcgcacatc cgcacgctgc tgctgacgtt cttctaccgg cagatgccgg agatgatcga gcgcggctac gtgtacatcg cgcagccgcc gctctacaag gtgaaggctg gccgcgacga gcgctacctg aaggacgaca ccgagctgaa cgcgcacatg ctgcgcctcg cgctgcaggg ctcggagctc gtgccgaccg agaacggcac accgatctcg ggcgacgcgc tcggcgagct tgcgcgctcg tatctgctcg cgcggggcgt ggtggagcgg ctgagccgcc tgtacgaccc ggccgcgctc gaggcggtca tggacggcgt cgcgatcgat ctgtcgagcg aggcgtcgac ggaggcgtcg gcgaaggcgc tggccgccat gctgtccgac gattcgaaga ccgaagtgcg cgtcgtgccc gcttacgatg cggtgcgcga gcagcgctcg ctgcgtgtcg agcgcacgca tcacggcaac gtgcgcgtat cggtgatcga cgaggagttc cagcacacgg ctgattatca gcaactcgta aacaccgcga atacgttcaa gggtttgatc ggcgcggggg cggcgatcaa gcgcggcgaa cggagctcga acgtggcgga cttcaaggaa gcgatgaagt ggctgatggc cgacgccgag cggaacatgt ctaagcagcg gtataagggg ttgggcgaga tgaaccccga gcagctgtgg gaaacgacga tggacccggc ggtgcggcgg ttgctgcgcg tgcagatcga ggatgcgatt gcggcggatg ggatctttac caccctcatg ggggacgagg tcgagccgcg acgggcgttt atcgagaata atgcgttgcg ggcggggaat attgatgttt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa