ButhA.00157.a

DNA gyrase, A subunit (EC 5.99.1.3) (BTH_I1632) (gyrA)

CENTER ID: ButhA.00157.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00157.a.A1.GE32731 full length 1 866
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00157.a.A1.PW33335 full length 1 866

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.4418
RefSeq: YP_442169.1
UniProt: Q2SY30

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDQFAKETLP TSLEEEMRKS YLDYAMSVIV GRALPDVRDG LKPVHRRVLF AMHELNNDWN RPYKKSARIV GDVIGKYHPH GDTAVYDTIV RMAQDFSLRY MLVDGQGNFG SVDGDNAAAM RYTEIRMAKI GHELLADIDK ETVDFGPNYD GSEMQPLILP SRIPNLLING SSGIAVGMAT NIPPHNLNEV VDACLYLLNN PQASVDELIE IIPAPDFPTA GIIYGVAGVR DGYRTGRGRV VMRAATHFEE IDRGQRMAII VDELPYQVNK RSLLERIAEL VNEKKLEGIS DIRDESDKSG MRVVIELKRG EVPEVVLNNL YKATQLQDTF GMNMVALVDN QPKLLNLREI LQCFLSHRRE VLTRRTVYEL RKARERGHVL EGLAVALANI DEFIAIIKAA PTPPIAKQEL MAKSWDSSLV REMLTRAEAE NAAAGGRAAY RPEGLSPAFG MQGDGLYRLS DTQAQEILQM RLQRLTGLEQ DKIIGEYRDV MAQIADLLDI LARPERITVM IGEELAAVKA EFGDARRSKI ELNATELNTE DLITPQDMVV TMSHAGYVKS QPLSEYRAQK RGGRGKQATQ MKEDDWIETL FIANTHDYIL CFSNRGRVYW VKVYEVPQGS RNSRGRPIVN MFPLQDGEKI NVVLPVKEFS ADKYVFMTTA LGTVKKTPLE AFSRPMKKGI IAVGLDDGDY LIGAAITDGA HDVMLFSDAG KAVRFDENDV RPMGREARGV RGMQLDDGQQ VIAMLVAGSE EQSVLTATEN GYGKRTPITE YTRHGRGTKG MIAIQTSERN GRVVAATLVD TEDQIMLITT AGVLIRTRVS EIREMGRATQ GVTLISLDEG TKLSGLQQIA EADADADDDA SEAGDA
NT Sequence
atggatcaat tcgccaaaga gaccctgccc acctccctcg aggaggaaat gcgcaagtcg tatctcgatt acgccatgag cgtgatcgtc ggacgtgccc ttccggatgt ccgcgatggc ttgaagcccg tgcaccggcg cgtgctgttc gcgatgcacg agttgaacaa cgactggaac cggccctaca agaagtccgc gcgtatcgtc ggcgacgtga tcggtaaata ccatccgcac ggcgacactg cggtctacga cacgatcgtc cggatggccc aggatttctc gctgcgctac atgctcgtcg acggccaggg caacttcggc tcggtcgacg gcgacaacgc ggccgcgatg cgctacaccg aaatccggat ggccaagatc ggccacgaac tgcttgcgga catcgacaag gaaaccgtcg acttcggccc gaactacgac ggcagcgaga tgcagccgct catcctgccg tcgcggattc cgaacctcct catcaacggt tcgtcgggga tcgcggtcgg catggcgacc aacattccgc cgcacaacct gaacgaggtc gtcgacgcat gcctgtacct gctgaacaat ccgcaggcga gcgtcgacga gctgatcgag atcatccccg cgcccgattt cccgacggcg ggcatcatct acggcgttgc cggcgtgcgc gatggctacc gcaccggccg cgggcgcgtc gtgatgcgcg cggccacgca cttcgaggag atcgaccgcg gccagcggat ggcgatcatc gtcgacgagc tgccgtacca ggtcaacaag cgctcgctgc tcgagcggat cgccgagctc gtcaacgaga agaagctcga gggcatctcc gacattcgcg acgagtcgga caagagcggc atgcgggtcg tgatcgagct caagcgcggc gaggtgcccg aggtcgtgct gaacaacctg tacaaggcga ctcagctgca ggacacgttc ggcatgaaca tggtcgcgct cgtcgacaac cagccgaagc tcctgaatct gagggagatc ctgcagtgct tcctgtcgca tcggcgcgaa gtgctgacgc ggcgcacagt ctacgaactg cgcaaggcgc gcgagcgcgg ccacgtgctc gaaggtctcg ccgtcgcgct cgcgaacatc gacgagttca tcgcgatcat caaggccgcg ccgacgccgc cgatcgcgaa gcaggagctg atggcgaagt cgtgggattc gtcgctcgtg cgcgagatgc tcacgcgcgc ggaggcggag aacgcggcgg cgggcgggcg cgcggcgtac cgcccggaag ggctgagccc ggcgttcggc atgcagggcg acggcctgta ccgtttgtcc gacacgcagg cgcaggaaat cctccagatg cgcctgcaac gcctgacggg cctcgagcag gacaagatca tcggcgaata tcgcgacgtg atggcgcaga tcgcggattt gctggatatc ctggcccgcc ccgagcggat cacggtgatg atcggcgaag aactggctgc ggtgaaggcc gagttcggcg atgcgcgccg ctcgaagatc gagctgaacg cgaccgagct gaacaccgaa gacctgatta ctccgcagga catggtggtc acgatgtcgc acgcgggcta tgtgaagtcg cagccgctgt ccgagtaccg cgcgcagaag cgcggtggcc gcggcaagca ggcgacgcag atgaaagaag acgactggat cgagacgctc ttcatcgcga acacgcacga ctacatcctg tgcttctcga accgcggccg cgtgtactgg gtcaaggtct acgaggtgcc gcagggctcg cgcaactcgc gcggccgtcc gatcgtcaac atgttcccgc tgcaggacgg cgagaagatc aacgtcgtgc tgcccgtgaa ggaattctcg gccgacaagt acgtgttcat gacgacggct ttgggcacgg tcaagaagac gccgctcgaa gcgttcagcc gcccgatgaa gaagggcatc atcgcggtcg gcctcgacga cggcgactat ctgatcggcg cggcgatcac cgacggtgcg cacgacgtga tgctgttctc cgacgccggc aaggcggtgc gcttcgacga gaacgacgtg cgtccgatgg gccgcgaggc gcgcggcgtg cgcgggatgc agctcgacga cgggcagcag gtgatcgcga tgctcgtcgc gggcagcgag gagcagtcgg tgctgaccgc gaccgagaac ggctacggca agcgcacgcc gatcaccgag tacacgcgcc acgggcgcgg cacgaagggg atgatcgcga tccagacgtc cgagcgcaac ggccgcgtcg ttgcggcgac gctcgtcgac accgaggatc agatcatgct gatcacgacg gcgggcgtgc tgatccgcac gcgcgtatcg gagatccgcg agatggggcg ggcgacgcaa ggtgttacac tcatcagtct cgatgaaggc accaagcttt cgggtctgca gcagatcgcc gaggcggacg ccgatgccga cgacgacgcg tcggaggccg gcgacgcctg a
Details for ButhA.00157.a.A1.GE32731
HARVESTED ON: 6/20/2011
SEQUENCED ON: 7/14/2011
EXPECTED MW: 98kDa
OBSERVED MW: 98kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 92
PERCENT COVERAGE: 33
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDQFAKETL PTSLEEEMRK SYLDYAMSVI VGRALPDVRD GLKPVHRRVL FAMHELNNDW NRPYKKSARI VGDVIGKYHP HGDTAVYDTI VRMAQDFSLR YMLVDGQGNF GSVDGDNAAA MRYTEIRMAK IGHELLADID KETVDFGPNY DGSEMQPLIL PSRIPNLLIN GSSGIAVGMA TNIPPHNLNE VVDACLYLLN NPQASVDELI EIIPAPDFPT AGIIYGVAGV RDGYRTGRGR VVMRAXXTSR RSTAXSXWRS SSXSCRTXST SARC
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggatc aattcgccaa agagaccctg cccacctccc tcgaggagga aatgcgcaag tcgtatctcg attacgccat gagcgtgatc gtcggacgtg cccttccgga tgtccgcgat ggcttgaagc ccgtgcaccg gcgcgtgctg ttcgcgatgc acgagttgaa caacgactgg aaccggccct acaagaagtc cgcgcgtatc gtcggcgacg tgatcggtaa ataccatccg cacggcgaca ctgcggtcta cgacacgatc gtccggatgg cccaggattt ctcgctgcgc tacatgctcg tcgacggcca gggcaacttc ggctcggtcg acggcgacaa cgcggccgcg atgcgctaca ccgaaatccg gatggccaag atcggccacg aactgcttgc ggacatcgac aaggaaaccg tcgacttcgg cccgaactac gacggcagcg agatgcagcc gctcatcctg ccgtcgcgga ttccgaacct cctcatcaac ggttcgtcgg ggatcgcggt cggcatggcg accaacattc cgccgcacaa cctgaacgag gtcgtcgacg catgcctgta cctgctgaac aatccgcagg cgagcgtcga cgagctgatc gagatcatcc ccgcgcccga tttcccgacg gcgggcatca tctacggcgt tgccggcgtg cgcgatggct accgcaccgg ccgcgggcgc gtcgtgatgc gcgcgncnng cacttcgagg agatcgaccg cgnncagcgn atggcgatca tcgtcganna gctgccgtac cnggtcnaca agcgctcgct gct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDQFAKETL PTSLEEEMRK SYLDYAMSVI VGRALPDVRD GLKPVHRRVL FAMHELNNDW NRPYKKSARI VGDVIGKYHP HGDTAVYDTI VRMAQDFSLR YMLVDGQGNF GSVDGDNAAA MRYTEIRMAK IGHELLADID KETVDFGPNY DGSEMQPLIL PSRIPNLLIN GSSGIAVGMA TNIPPHNLNE VVDACLYLLN NPQASVDELI EIIPAPDFPT AGIIYGVAGV RDGYRTGRGR VVMRAATHFE EIDRGQRMAI IVDELPYQVN KRSLLERIAE LVNEKKLEGI SDIRDESDKS GMRVVIELKR GEVPEVVLNN LYKATQLQDT FGMNMVALVD NQPKLLNLRE ILQCFLSHRR EVLTRRTVYE LRKARERGHV LEGLAVALAN IDEFIAIIKA APTPPIAKQE LMAKSWDSSL VREMLTRAEA ENAAAGGRAA YRPEGLSPAF GMQGDGLYRL SDTQAQEILQ MRLQRLTGLE QDKIIGEYRD VMAQIADLLD ILARPERITV MIGEELAAVK AEFGDARRSK IELNATELNT EDLITPQDMV VTMSHAGYVK SQPLSEYRAQ KRGGRGKQAT QMKEDDWIET LFIANTHDYI LCFSNRGRVY WVKVYEVPQG SRNSRGRPIV NMFPLQDGEK INVVLPVKEF SADKYVFMTT ALGTVKKTPL EAFSRPMKKG IIAVGLDDGD YLIGAAITDG AHDVMLFSDA GKAVRFDEND VRPMGREARG VRGMQLDDGQ QVIAMLVAGS EEQSVLTATE NGYGKRTPIT EYTRHGRGTK GMIAIQTSER NGRVVAATLV DTEDQIMLIT TAGVLIRTRV SEIREMGRAT QGVTLISLDE GTKLSGLQQI AEADADADDD ASEAGDA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatcaattc gccaaagaga ccctgcccac ctccctcgag gaggaaatgc gcaagtcgta tctcgattac gccatgagcg tgatcgtcgg acgtgccctt ccggatgtcc gcgatggctt gaagcccgtg caccggcgcg tgctgttcgc gatgcacgag ttgaacaacg actggaaccg gccctacaag aagtccgcgc gtatcgtcgg cgacgtgatc ggtaaatacc atccgcacgg cgacactgcg gtctacgaca cgatcgtccg gatggcccag gatttctcgc tgcgctacat gctcgtcgac ggccagggca acttcggctc ggtcgacggc gacaacgcgg ccgcgatgcg ctacaccgaa atccggatgg ccaagatcgg ccacgaactg cttgcggaca tcgacaagga aaccgtcgac ttcggcccga actacgacgg cagcgagatg cagccgctca tcctgccgtc gcggattccg aacctcctca tcaacggttc gtcggggatc gcggtcggca tggcgaccaa cattccgccg cacaacctga acgaggtcgt cgacgcatgc ctgtacctgc tgaacaatcc gcaggcgagc gtcgacgagc tgatcgagat catccccgcg cccgatttcc cgacggcggg catcatctac ggcgttgccg gcgtgcgcga tggctaccgc accggccgcg ggcgcgtcgt gatgcgcgcg gccacgcact tcgaggagat cgaccgcggc cagcggatgg cgatcatcgt cgacgagctg ccgtaccagg tcaacaagcg ctcgctgctc gagcggatcg ccgagctcgt caacgagaag aagctcgagg gcatctccga cattcgcgac gagtcggaca agagcggcat gcgggtcgtg atcgagctca agcgcggcga ggtgcccgag gtcgtgctga acaacctgta caaggcgact cagctgcagg acacgttcgg catgaacatg gtcgcgctcg tcgacaacca gccgaagctc ctgaatctga gggagatcct gcagtgcttc ctgtcgcatc ggcgcgaagt gctgacgcgg cgcacagtct acgaactgcg caaggcgcgc gagcgcggcc acgtgctcga aggtctcgcc gtcgcgctcg cgaacatcga cgagttcatc gcgatcatca aggccgcgcc gacgccgccg atcgcgaagc aggagctgat ggcgaagtcg tgggattcgt cgctcgtgcg cgagatgctc acgcgcgcgg aggcggagaa cgcggcggcg ggcgggcgcg cggcgtaccg cccggaaggg ctgagcccgg cgttcggcat gcagggcgac ggcctgtacc gtttgtccga cacgcaggcg caggaaatcc tccagatgcg cctgcaacgc ctgacgggcc tcgagcagga caagatcatc ggcgaatatc gcgacgtgat ggcgcagatc gcggatttgc tggatatcct ggcccgcccc gagcggatca cggtgatgat cggcgaagaa ctggctgcgg tgaaggccga gttcggcgat gcgcgccgct cgaagatcga gctgaacgcg accgagctga acaccgaaga cctgattact ccgcaggaca tggtggtcac gatgtcgcac gcgggctatg tgaagtcgca gccgctgtcc gagtaccgcg cgcagaagcg cggtggccgc ggcaagcagg cgacgcagat gaaagaagac gactggatcg agacgctctt catcgcgaac acgcacgact acatcctgtg cttctcgaac cgcggccgcg tgtactgggt caaggtctac gaggtgccgc agggctcgcg caactcgcgc ggccgtccga tcgtcaacat gttcccgctg caggacggcg agaagatcaa cgtcgtgctg cccgtgaagg aattctcggc cgacaagtac gtgttcatga cgacggcttt gggcacggtc aagaagacgc cgctcgaagc gttcagccgc ccgatgaaga agggcatcat cgcggtcggc ctcgacgacg gcgactatct gatcggcgcg gcgatcaccg acggtgcgca cgacgtgatg ctgttctccg acgccggcaa ggcggtgcgc ttcgacgaga acgacgtgcg tccgatgggc cgcgaggcgc gcggcgtgcg cgggatgcag ctcgacgacg ggcagcaggt gatcgcgatg ctcgtcgcgg gcagcgagga gcagtcggtg ctgaccgcga ccgagaacgg ctacggcaag cgcacgccga tcaccgagta cacgcgccac gggcgcggca cgaaggggat gatcgcgatc cagacgtccg agcgcaacgg ccgcgtcgtt gcggcgacgc tcgtcgacac cgaggatcag atcatgctga tcacgacggc gggcgtgctg atccgcacgc gcgtatcgga gatccgcgag atggggcggg cgacgcaagg tgttacactc atcagtctcg atgaaggcac caagctttcg ggtctgcagc agatcgccga ggcggacgcc gatgccgacg acgacgcgtc ggaggccggc gacgcctaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for ButhA.00157.a.A1.PW33335
PURIFICATION DATe: 8/19/2011
CONCENTRATION: 34.74mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 100µl
PERCENT IDENTITY: 92
PERCENT COVERAGE: 33
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDQFAKETL PTSLEEEMRK SYLDYAMSVI VGRALPDVRD GLKPVHRRVL FAMHELNNDW NRPYKKSARI VGDVIGKYHP HGDTAVYDTI VRMAQDFSLR YMLVDGQGNF GSVDGDNAAA MRYTEIRMAK IGHELLADID KETVDFGPNY DGSEMQPLIL PSRIPNLLIN GSSGIAVGMA TNIPPHNLNE VVDACLYLLN NPQASVDELI EIIPAPDFPT AGIIYGVAGV RDGYRTGRGR VVMRAXXTSR RSTAXSXWRS SSXSCRTXST SARC
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggatc aattcgccaa agagaccctg cccacctccc tcgaggagga aatgcgcaag tcgtatctcg attacgccat gagcgtgatc gtcggacgtg cccttccgga tgtccgcgat ggcttgaagc ccgtgcaccg gcgcgtgctg ttcgcgatgc acgagttgaa caacgactgg aaccggccct acaagaagtc cgcgcgtatc gtcggcgacg tgatcggtaa ataccatccg cacggcgaca ctgcggtcta cgacacgatc gtccggatgg cccaggattt ctcgctgcgc tacatgctcg tcgacggcca gggcaacttc ggctcggtcg acggcgacaa cgcggccgcg atgcgctaca ccgaaatccg gatggccaag atcggccacg aactgcttgc ggacatcgac aaggaaaccg tcgacttcgg cccgaactac gacggcagcg agatgcagcc gctcatcctg ccgtcgcgga ttccgaacct cctcatcaac ggttcgtcgg ggatcgcggt cggcatggcg accaacattc cgccgcacaa cctgaacgag gtcgtcgacg catgcctgta cctgctgaac aatccgcagg cgagcgtcga cgagctgatc gagatcatcc ccgcgcccga tttcccgacg gcgggcatca tctacggcgt tgccggcgtg cgcgatggct accgcaccgg ccgcgggcgc gtcgtgatgc gcgcgncnng cacttcgagg agatcgaccg cgnncagcgn atggcgatca tcgtcganna gctgccgtac cnggtcnaca agcgctcgct gct
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDQFAKETL PTSLEEEMRK SYLDYAMSVI VGRALPDVRD GLKPVHRRVL FAMHELNNDW NRPYKKSARI VGDVIGKYHP HGDTAVYDTI VRMAQDFSLR YMLVDGQGNF GSVDGDNAAA MRYTEIRMAK IGHELLADID KETVDFGPNY DGSEMQPLIL PSRIPNLLIN GSSGIAVGMA TNIPPHNLNE VVDACLYLLN NPQASVDELI EIIPAPDFPT AGIIYGVAGV RDGYRTGRGR VVMRAATHFE EIDRGQRMAI IVDELPYQVN KRSLLERIAE LVNEKKLEGI SDIRDESDKS GMRVVIELKR GEVPEVVLNN LYKATQLQDT FGMNMVALVD NQPKLLNLRE ILQCFLSHRR EVLTRRTVYE LRKARERGHV LEGLAVALAN IDEFIAIIKA APTPPIAKQE LMAKSWDSSL VREMLTRAEA ENAAAGGRAA YRPEGLSPAF GMQGDGLYRL SDTQAQEILQ MRLQRLTGLE QDKIIGEYRD VMAQIADLLD ILARPERITV MIGEELAAVK AEFGDARRSK IELNATELNT EDLITPQDMV VTMSHAGYVK SQPLSEYRAQ KRGGRGKQAT QMKEDDWIET LFIANTHDYI LCFSNRGRVY WVKVYEVPQG SRNSRGRPIV NMFPLQDGEK INVVLPVKEF SADKYVFMTT ALGTVKKTPL EAFSRPMKKG IIAVGLDDGD YLIGAAITDG AHDVMLFSDA GKAVRFDEND VRPMGREARG VRGMQLDDGQ QVIAMLVAGS EEQSVLTATE NGYGKRTPIT EYTRHGRGTK GMIAIQTSER NGRVVAATLV DTEDQIMLIT TAGVLIRTRV SEIREMGRAT QGVTLISLDE GTKLSGLQQI AEADADADDD ASEAGDA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGATCAATTC GCCAAAGAGA CCCTGCCCAC CTCCCTCGAG GAGGAAATGC GCAAGTCGTA TCTCGATTAC GCCATGAGCG TGATCGTCGG ACGTGCCCTT CCGGATGTCC GCGATGGCTT GAAGCCCGTG CACCGGCGCG TGCTGTTCGC GATGCACGAG TTGAACAACG ACTGGAACCG GCCCTACAAG AAGTCCGCGC GTATCGTCGG CGACGTGATC GGTAAATACC ATCCGCACGG CGACACTGCG GTCTACGACA CGATCGTCCG GATGGCCCAG GATTTCTCGC TGCGCTACAT GCTCGTCGAC GGCCAGGGCA ACTTCGGCTC GGTCGACGGC GACAACGCGG CCGCGATGCG CTACACCGAA ATCCGGATGG CCAAGATCGG CCACGAACTG CTTGCGGACA TCGACAAGGA AACCGTCGAC TTCGGCCCGA ACTACGACGG CAGCGAGATG CAGCCGCTCA TCCTGCCGTC GCGGATTCCG AACCTCCTCA TCAACGGTTC GTCGGGGATC GCGGTCGGCA TGGCGACCAA CATTCCGCCG CACAACCTGA ACGAGGTCGT CGACGCATGC CTGTACCTGC TGAACAATCC GCAGGCGAGC GTCGACGAGC TGATCGAGAT CATCCCCGCG CCCGATTTCC CGACGGCGGG CATCATCTAC GGCGTTGCCG GCGTGCGCGA TGGCTACCGC ACCGGCCGCG GGCGCGTCGT GATGCGCGCG GCCACGCACT TCGAGGAGAT CGACCGCGGC CAGCGGATGG CGATCATCGT CGACGAGCTG CCGTACCAGG TCAACAAGCG CTCGCTGCTC GAGCGGATCG CCGAGCTCGT CAACGAGAAG AAGCTCGAGG GCATCTCCGA CATTCGCGAC GAGTCGGACA AGAGCGGCAT GCGGGTCGTG ATCGAGCTCA AGCGCGGCGA GGTGCCCGAG GTCGTGCTGA ACAACCTGTA CAAGGCGACT CAGCTGCAGG ACACGTTCGG CATGAACATG GTCGCGCTCG TCGACAACCA GCCGAAGCTC CTGAATCTGA GGGAGATCCT GCAGTGCTTC CTGTCGCATC GGCGCGAAGT GCTGACGCGG CGCACAGTCT ACGAACTGCG CAAGGCGCGC GAGCGCGGCC ACGTGCTCGA AGGTCTCGCC GTCGCGCTCG CGAACATCGA CGAGTTCATC GCGATCATCA AGGCCGCGCC GACGCCGCCG ATCGCGAAGC AGGAGCTGAT GGCGAAGTCG TGGGATTCGT CGCTCGTGCG CGAGATGCTC ACGCGCGCGG AGGCGGAGAA CGCGGCGGCG GGCGGGCGCG CGGCGTACCG CCCGGAAGGG CTGAGCCCGG CGTTCGGCAT GCAGGGCGAC GGCCTGTACC GTTTGTCCGA CACGCAGGCG CAGGAAATCC TCCAGATGCG CCTGCAACGC CTGACGGGCC TCGAGCAGGA CAAGATCATC GGCGAATATC GCGACGTGAT GGCGCAGATC GCGGATTTGC TGGATATCCT GGCCCGCCCC GAGCGGATCA CGGTGATGAT CGGCGAAGAA CTGGCTGCGG TGAAGGCCGA GTTCGGCGAT GCGCGCCGCT CGAAGATCGA GCTGAACGCG ACCGAGCTGA ACACCGAAGA CCTGATTACT CCGCAGGACA TGGTGGTCAC GATGTCGCAC GCGGGCTATG TGAAGTCGCA GCCGCTGTCC GAGTACCGCG CGCAGAAGCG CGGTGGCCGC GGCAAGCAGG CGACGCAGAT GAAAGAAGAC GACTGGATCG AGACGCTCTT CATCGCGAAC ACGCACGACT ACATCCTGTG CTTCTCGAAC CGCGGCCGCG TGTACTGGGT CAAGGTCTAC GAGGTGCCGC AGGGCTCGCG CAACTCGCGC GGCCGTCCGA TCGTCAACAT GTTCCCGCTG CAGGACGGCG AGAAGATCAA CGTCGTGCTG CCCGTGAAGG AATTCTCGGC CGACAAGTAC GTGTTCATGA CGACGGCTTT GGGCACGGTC AAGAAGACGC CGCTCGAAGC GTTCAGCCGC CCGATGAAGA AGGGCATCAT CGCGGTCGGC CTCGACGACG GCGACTATCT GATCGGCGCG GCGATCACCG ACGGTGCGCA CGACGTGATG CTGTTCTCCG ACGCCGGCAA GGCGGTGCGC TTCGACGAGA ACGACGTGCG TCCGATGGGC CGCGAGGCGC GCGGCGTGCG CGGGATGCAG CTCGACGACG GGCAGCAGGT GATCGCGATG CTCGTCGCGG GCAGCGAGGA GCAGTCGGTG CTGACCGCGA CCGAGAACGG CTACGGCAAG CGCACGCCGA TCACCGAGTA CACGCGCCAC GGGCGCGGCA CGAAGGGGAT GATCGCGATC CAGACGTCCG AGCGCAACGG CCGCGTCGTT GCGGCGACGC TCGTCGACAC CGAGGATCAG ATCATGCTGA TCACGACGGC GGGCGTGCTG ATCCGCACGC GCGTATCGGA GATCCGCGAG ATGGGGCGGG CGACGCAAGG TGTTACACTC ATCAGTCTCG ATGAAGGCAC CAAGCTTTCG GGTCTGCAGC AGATCGCCGA GGCGGACGCC GATGCCGACG ACGACGCGTC GGAGGCCGGC GACGCCTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA