ButhA.00153.a

DNA primase (BTH_II0620)

CENTER ID: ButhA.00153.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00153.a.A1.GE32730 full length 1 625
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00153.a.A1.PW33334 full length 1 625

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.705
RefSeq: YP_438820.1
UniProt: Q2T7M9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIPHSFLQDL LNRVDIVDVV GRYVQLKKGG ANFMGLCPFH NEKSPSFTVS PTKQFYHCFG CGAHGTAIGF LMEHAGLTFP EAVQELAQSV GLTVPHEPSP MRGAGGGSGE GYAPPAASKS VTTALSDVMQ TACDYYRKQL RGAPNAIQYL KRRGLTGEIA LRFGLGYAPD GWQNLEAAFD DYRHDSLVDA GLVIVSDKTD ANGAARRYDR FRERIMFPIR NVKGQVIGFG GRVLDGGEPK YLNSPETPLF NKGSELYGLF EARLAIREHK YALVVEGYMD VVALAQLGFP NAVATLGTAC TPVHVQKLLR QTDTVVFSFD GDAAGRRAAR RALEACLPHA ADNRTIRFLF LPPEHDPDSY VREFGTDAFS EQVKRAMPLS QFLLNEATAG KELDQPEGRA KALFDAKPLL QALPANALRA QIMHMFADRL RIPFDEVAAL AEVDARIAAP ARQAPARSER RRVTDSEKRA LRNLVMHPRI AAVLDDESAA TLRALPRIGE LFDEVIGHAL ALGEGAEFRL LSDVLRNSAN APTYDEIFRE ILVYDENVRD LMQQNPESDA AQEGLRERER IAGEELQAAV LKMRYDACCD RLNSLSKRSA PSSEELAEIK DLHQNLADMK RRLGL
NT Sequence
gtgattccgc attcgttcct gcaggatttg ctgaaccgcg tcgacatcgt cgacgtggtg ggccggtacg tgcagctcaa gaagggcggc gccaatttca tggggctgtg cccgtttcac aacgagaaga gcccgtcgtt tacggtcagt ccgaccaagc agttctatca ttgcttcggc tgcggtgcgc acggcaccgc gatcggcttc ctgatggagc acgcggggct cacgtttccc gaggcggtgc aggagctcgc gcagtcggtc gggctcacgg tgccgcatga accgtcgccg atgcgaggcg cgggcggcgg ctcgggcgag ggctacgcgc cgcccgccgc gtcgaaatcg gtcacgaccg cgctgtccga cgtgatgcag accgcctgcg actattaccg caagcagctg cgcggtgcgc cgaacgcgat ccagtatctg aagcggcgcg gcctgacggg cgagatcgcg ctgcgcttcg ggctcggcta cgcgccggac ggctggcaga acctcgaggc ggcgttcgac gattatcgtc acgattcgct cgtcgacgcc ggcctcgtga tcgtcagcga taagaccgac gcgaacggcg ccgcgcgccg ctacgaccgg ttccgcgagc ggatcatgtt cccgatccgc aacgtgaagg ggcaggtcat cggctttggc ggccgggtgc tggacggcgg cgagcccaag tatctgaatt cgcccgagac gccgctgttc aacaagggca gcgagttgta cggcctgttc gaggcgaggc tcgcgattcg cgagcacaag tacgcgctcg tcgtcgaagg gtacatggac gtcgtcgcgc tcgcgcagct cggcttcccg aatgcggtgg cgaccctcgg caccgcgtgc acgccggtcc acgtgcagaa gctgctgcgg cagacggaca cggtcgtctt cagcttcgac ggcgatgcgg ccggccggcg cgccgcgcgg cgcgcgctcg aggcgtgcct gccgcacgcg gcggacaacc ggacgatccg cttcctcttc ctgcccccgg agcacgatcc ggacagctac gtgcgggaat tcgggacgga tgcgttttcg gagcaggtga agcgggcgat gccgctgtcg cagttcctgc tgaacgaagc gacggcgggt aaggagctcg accagccgga agggcgcgcg aaggcgctgt tcgatgcgaa gccgctgctg caggcgctgc ccgcgaacgc gttgcgcgcg cagatcatgc atatgttcgc ggaccggctg cggattccgt tcgacgaagt cgccgcgctc gccgaggtgg acgcgcggat cgccgcgccg gcccgccagg cgcccgcccg cagcgagcgg cggcgcgtga cggacagcga gaagcgcgcg ttgcgcaacc tggtgatgca tccccgtatc gcggcggtgc tcgacgacga gagtgctgcg acgctgcggg cgctgccgag aatcggggaa ctgttcgacg aggtaatcgg ccacgcgctc gcgctcggag aaggggcgga attcaggctt ttgtccgacg tgctacgcaa ttcggccaac gcgcccacct acgacgaaat tttccgggaa attctggtct atgatgaaaa tgtccgcgac ttgatgcagc agaatccgga aagcgacgcg gcgcaggaag gattgcgcga gcgagaacgg atcgcaggtg aggagcttca ggcagcggtg ctcaagatgc gctatgacgc gtgctgcgac aggctcaaca gcctgtcgaa gcgctcggcg ccctcttccg aggaattggc ggaaatcaag gacttgcatc agaacctggc agacatgaaa cggaggctcg ggctgtga
Details for ButhA.00153.a.A1.GE32730
HARVESTED ON: 6/20/2011
SEQUENCED ON: 7/14/2011
EXPECTED MW: 71kDa
OBSERVED MW: 71kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 70
PERCENT COVERAGE: 62
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVIPHSFLQ DLLNRVDIVD VVGRYVQLKK GGANFMGLCP FHNEKSPSFT VSPTKQFYHC FGCGAHGTAI GFLMEHAGLT FPEAVQELAQ SVGLTVPHEP SPMRGAGGGS GEGYAPPAAS KSVTTALSDV MQTACDYYRK QLRGAPNAIQ YLKRRGLTGE IALRFGLGYA PDGWQNLEAA FDDYRHDSLV DAXLVIVSDK TDANGAARRY DRXRERIMFP IRNVKGXVIG FGGRXLDGGE PKYLNSPXTP XFTRXASCXX XXXXXSRXAX XXTRSSXXXX XXRXAXXAXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXRA RGVXAARGGQ PDDPXXLXAP GARSGQXRAG IRDGCVFGAG EAGDAAVAVX AERSDGG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga ttccgcattc gttcctgcag gatttgctga accgcgtcga catcgtcgac gtggtgggcc ggtacgtgca gctcaagaag ggcggcgcca atttcatggg gctgtgcccg tttcacaacg agaagagccc gtcgtttacg gtcagtccga ccaagcagtt ctatcattgc ttcggctgcg gtgcgcacgg caccgcgatc ggcttcctga tggagcacgc ggggctcacg tttcccgagg cggtgcagga gctcgcgcag tcggtcgggc tcacggtgcc gcatgaaccg tcgccgatgc gaggcgcggg cggcggctcg ggcgagggct acgcgccgcc cgccgcgtcg aaatcggtca cgaccgcgct gtccgacgtg atgcagaccg cctgcgacta ttaccgcaag cagctgcgcg gtgcgccgaa cgcgatccag tatctgaagc ggcgcggcct gacgggcgag atcgcgctgc gcttcgggct cggctacgcg ccggacggct ggcagaacct cgaggcggcg ttcgacgatt atcgtcacga ttcgctcgtc gacgccngcc tcgtgatcgt cagcgataag accgacgcga acggcgccgc gcgccgctac gaccgnntcc gcgagcggat catgttcccg atccgcaacg tgaaggggcn ggtcatcggc tttggcggcc gggngctgga cggcggcgag cccaagtatc tgaattcgcc nnagacgccn ntgttcacaa ggnnagcgag ttgtanggnn ngtnnnangc nangctcgcg antngcnnnn nnnantacnc gctcgtcgnc nannnnnnnt ngnnntcgtc ncgcnnnnnc agctcnnnnn nccnnangnn nnnnnancnn nnnnnnnnnn nnnnnnncnn nnnnnnnnna nntgcnnnnn nnnnnnnnnn nnnnnnntnn nnttnnnngn nnnnnnnnnn gnnnnncnnn nnggcgcgct cgaggcgtnc ntgccgcacg cggcggacaa ccggacgatc cgnttcntct tnctgccccc ggagcacgat cnggacagnt acgtgcggga attcgggacg gatgcgtttt cggagcaggt gaagcgggcg atgccgctgt cgcagttcnt gctgaacgaa gcgacggcgg gtaaggagct cgaccagccg gaagggcgcg cgaaggcgct gttcgatgcg aagccgctgc tgcaggcgct gcccgcgaac gcgttgcgcg cgcagatcat gcatatgttc gcggaccggc tgcggattcc gttcgacgaa gtcgccgcgc tcgccgaggt ggacgcgcgg atcgccgcgc cggcccgcca ggcgcccgcc cgcagcgagc ggcggcgcgt gacggacagc gagaagcgcg cgttgcgcaa cctggtgatg catccccgta tcgcggcggt gctcgacgac gagagtgctg cgacgctgcg ggcgctgccg agaatcgggg aactgttcga cgaggtaatc ggccacgcgc tcgcgctcgg agaaggggcg gaattcaggc ttttgtccga cgtgctacgc aattcggcca acgcgcccac ctacgacgaa attttccggg aaattctggt ctatgatgaa aatgtccgcg acttgatgca gcagaatccg gaaagcgacg cggcgcagga aggattgcgc gagcgagaac ggatcgcagg tgaggagctt caggcagcgg tgctcaagat gcgctatgac gcgtgctgcg acaggctcaa cagcctgtcg aagcgctcgg cgccctcttc cgaggaattg gcggaaatca aggacttgca tcagaacctg gcagacatga aacggaggct cgggctgt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVIPHSFLQ DLLNRVDIVD VVGRYVQLKK GGANFMGLCP FHNEKSPSFT VSPTKQFYHC FGCGAHGTAI GFLMEHAGLT FPEAVQELAQ SVGLTVPHEP SPMRGAGGGS GEGYAPPAAS KSVTTALSDV MQTACDYYRK QLRGAPNAIQ YLKRRGLTGE IALRFGLGYA PDGWQNLEAA FDDYRHDSLV DAGLVIVSDK TDANGAARRY DRFRERIMFP IRNVKGQVIG FGGRVLDGGE PKYLNSPETP LFNKGSELYG LFEARLAIRE HKYALVVEGY MDVVALAQLG FPNAVATLGT ACTPVHVQKL LRQTDTVVFS FDGDAAGRRA ARRALEACLP HAADNRTIRF LFLPPEHDPD SYVREFGTDA FSEQVKRAMP LSQFLLNEAT AGKELDQPEG RAKALFDAKP LLQALPANAL RAQIMHMFAD RLRIPFDEVA ALAEVDARIA APARQAPARS ERRRVTDSEK RALRNLVMHP RIAAVLDDES AATLRALPRI GELFDEVIGH ALALGEGAEF RLLSDVLRNS ANAPTYDEIF REILVYDENV RDLMQQNPES DAAQEGLRER ERIAGEELQA AVLKMRYDAC CDRLNSLSKR SAPSSEELAE IKDLHQNLAD MKRRLGL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgattccg cattcgttcc tgcaggattt gctgaaccgc gtcgacatcg tcgacgtggt gggccggtac gtgcagctca agaagggcgg cgccaatttc atggggctgt gcccgtttca caacgagaag agcccgtcgt ttacggtcag tccgaccaag cagttctatc attgcttcgg ctgcggtgcg cacggcaccg cgatcggctt cctgatggag cacgcggggc tcacgtttcc cgaggcggtg caggagctcg cgcagtcggt cgggctcacg gtgccgcatg aaccgtcgcc gatgcgaggc gcgggcggcg gctcgggcga gggctacgcg ccgcccgccg cgtcgaaatc ggtcacgacc gcgctgtccg acgtgatgca gaccgcctgc gactattacc gcaagcagct gcgcggtgcg ccgaacgcga tccagtatct gaagcggcgc ggcctgacgg gcgagatcgc gctgcgcttc gggctcggct acgcgccgga cggctggcag aacctcgagg cggcgttcga cgattatcgt cacgattcgc tcgtcgacgc cggcctcgtg atcgtcagcg ataagaccga cgcgaacggc gccgcgcgcc gctacgaccg gttccgcgag cggatcatgt tcccgatccg caacgtgaag gggcaggtca tcggctttgg cggccgggtg ctggacggcg gcgagcccaa gtatctgaat tcgcccgaga cgccgctgtt caacaagggc agcgagttgt acggcctgtt cgaggcgagg ctcgcgattc gcgagcacaa gtacgcgctc gtcgtcgaag ggtacatgga cgtcgtcgcg ctcgcgcagc tcggcttccc gaatgcggtg gcgaccctcg gcaccgcgtg cacgccggtc cacgtgcaga agctgctgcg gcagacggac acggtcgtct tcagcttcga cggcgatgcg gccggccggc gcgccgcgcg gcgcgcgctc gaggcgtgcc tgccgcacgc ggcggacaac cggacgatcc gcttcctctt cctgcccccg gagcacgatc cggacagcta cgtgcgggaa ttcgggacgg atgcgttttc ggagcaggtg aagcgggcga tgccgctgtc gcagttcctg ctgaacgaag cgacggcggg taaggagctc gaccagccgg aagggcgcgc gaaggcgctg ttcgatgcga agccgctgct gcaggcgctg cccgcgaacg cgttgcgcgc gcagatcatg catatgttcg cggaccggct gcggattccg ttcgacgaag tcgccgcgct cgccgaggtg gacgcgcgga tcgccgcgcc ggcccgccag gcgcccgccc gcagcgagcg gcggcgcgtg acggacagcg agaagcgcgc gttgcgcaac ctggtgatgc atccccgtat cgcggcggtg ctcgacgacg agagtgctgc gacgctgcgg gcgctgccga gaatcgggga actgttcgac gaggtaatcg gccacgcgct cgcgctcgga gaaggggcgg aattcaggct tttgtccgac gtgctacgca attcggccaa cgcgcccacc tacgacgaaa ttttccggga aattctggtc tatgatgaaa atgtccgcga cttgatgcag cagaatccgg aaagcgacgc ggcgcaggaa ggattgcgcg agcgagaacg gatcgcaggt gaggagcttc aggcagcggt gctcaagatg cgctatgacg cgtgctgcga caggctcaac agcctgtcga agcgctcggc gccctcttcc gaggaattgg cggaaatcaa ggacttgcat cagaacctgg cagacatgaa acggaggctc gggctgtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for ButhA.00153.a.A1.PW33334
PURIFICATION DATe: 8/19/2011
CONCENTRATION: 21.46mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 12
VIAL VOLUME: 200µl
PERCENT IDENTITY: 70
PERCENT COVERAGE: 62
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVIPHSFLQ DLLNRVDIVD VVGRYVQLKK GGANFMGLCP FHNEKSPSFT VSPTKQFYHC FGCGAHGTAI GFLMEHAGLT FPEAVQELAQ SVGLTVPHEP SPMRGAGGGS GEGYAPPAAS KSVTTALSDV MQTACDYYRK QLRGAPNAIQ YLKRRGLTGE IALRFGLGYA PDGWQNLEAA FDDYRHDSLV DAXLVIVSDK TDANGAARRY DRXRERIMFP IRNVKGXVIG FGGRXLDGGE PKYLNSPXTP XFTRXASCXX XXXXXSRXAX XXTRSSXXXX XXRXAXXAXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXRA RGVXAARGGQ PDDPXXLXAP GARSGQXRAG IRDGCVFGAG EAGDAAVAVX AERSDGG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga ttccgcattc gttcctgcag gatttgctga accgcgtcga catcgtcgac gtggtgggcc ggtacgtgca gctcaagaag ggcggcgcca atttcatggg gctgtgcccg tttcacaacg agaagagccc gtcgtttacg gtcagtccga ccaagcagtt ctatcattgc ttcggctgcg gtgcgcacgg caccgcgatc ggcttcctga tggagcacgc ggggctcacg tttcccgagg cggtgcagga gctcgcgcag tcggtcgggc tcacggtgcc gcatgaaccg tcgccgatgc gaggcgcggg cggcggctcg ggcgagggct acgcgccgcc cgccgcgtcg aaatcggtca cgaccgcgct gtccgacgtg atgcagaccg cctgcgacta ttaccgcaag cagctgcgcg gtgcgccgaa cgcgatccag tatctgaagc ggcgcggcct gacgggcgag atcgcgctgc gcttcgggct cggctacgcg ccggacggct ggcagaacct cgaggcggcg ttcgacgatt atcgtcacga ttcgctcgtc gacgccngcc tcgtgatcgt cagcgataag accgacgcga acggcgccgc gcgccgctac gaccgnntcc gcgagcggat catgttcccg atccgcaacg tgaaggggcn ggtcatcggc tttggcggcc gggngctgga cggcggcgag cccaagtatc tgaattcgcc nnagacgccn ntgttcacaa ggnnagcgag ttgtanggnn ngtnnnangc nangctcgcg antngcnnnn nnnantacnc gctcgtcgnc nannnnnnnt ngnnntcgtc ncgcnnnnnc agctcnnnnn nccnnangnn nnnnnancnn nnnnnnnnnn nnnnnnncnn nnnnnnnnna nntgcnnnnn nnnnnnnnnn nnnnnnntnn nnttnnnngn nnnnnnnnnn gnnnnncnnn nnggcgcgct cgaggcgtnc ntgccgcacg cggcggacaa ccggacgatc cgnttcntct tnctgccccc ggagcacgat cnggacagnt acgtgcggga attcgggacg gatgcgtttt cggagcaggt gaagcgggcg atgccgctgt cgcagttcnt gctgaacgaa gcgacggcgg gtaaggagct cgaccagccg gaagggcgcg cgaaggcgct gttcgatgcg aagccgctgc tgcaggcgct gcccgcgaac gcgttgcgcg cgcagatcat gcatatgttc gcggaccggc tgcggattcc gttcgacgaa gtcgccgcgc tcgccgaggt ggacgcgcgg atcgccgcgc cggcccgcca ggcgcccgcc cgcagcgagc ggcggcgcgt gacggacagc gagaagcgcg cgttgcgcaa cctggtgatg catccccgta tcgcggcggt gctcgacgac gagagtgctg cgacgctgcg ggcgctgccg agaatcgggg aactgttcga cgaggtaatc ggccacgcgc tcgcgctcgg agaaggggcg gaattcaggc ttttgtccga cgtgctacgc aattcggcca acgcgcccac ctacgacgaa attttccggg aaattctggt ctatgatgaa aatgtccgcg acttgatgca gcagaatccg gaaagcgacg cggcgcagga aggattgcgc gagcgagaac ggatcgcagg tgaggagctt caggcagcgg tgctcaagat gcgctatgac gcgtgctgcg acaggctcaa cagcctgtcg aagcgctcgg cgccctcttc cgaggaattg gcggaaatca aggacttgca tcagaacctg gcagacatga aacggaggct cgggctgt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVIPHSFLQ DLLNRVDIVD VVGRYVQLKK GGANFMGLCP FHNEKSPSFT VSPTKQFYHC FGCGAHGTAI GFLMEHAGLT FPEAVQELAQ SVGLTVPHEP SPMRGAGGGS GEGYAPPAAS KSVTTALSDV MQTACDYYRK QLRGAPNAIQ YLKRRGLTGE IALRFGLGYA PDGWQNLEAA FDDYRHDSLV DAGLVIVSDK TDANGAARRY DRFRERIMFP IRNVKGQVIG FGGRVLDGGE PKYLNSPETP LFNKGSELYG LFEARLAIRE HKYALVVEGY MDVVALAQLG FPNAVATLGT ACTPVHVQKL LRQTDTVVFS FDGDAAGRRA ARRALEACLP HAADNRTIRF LFLPPEHDPD SYVREFGTDA FSEQVKRAMP LSQFLLNEAT AGKELDQPEG RAKALFDAKP LLQALPANAL RAQIMHMFAD RLRIPFDEVA ALAEVDARIA APARQAPARS ERRRVTDSEK RALRNLVMHP RIAAVLDDES AATLRALPRI GELFDEVIGH ALALGEGAEF RLLSDVLRNS ANAPTYDEIF REILVYDENV RDLMQQNPES DAAQEGLRER ERIAGEELQA AVLKMRYDAC CDRLNSLSKR SAPSSEELAE IKDLHQNLAD MKRRLGL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGATTCCG CATTCGTTCC TGCAGGATTT GCTGAACCGC GTCGACATCG TCGACGTGGT GGGCCGGTAC GTGCAGCTCA AGAAGGGCGG CGCCAATTTC ATGGGGCTGT GCCCGTTTCA CAACGAGAAG AGCCCGTCGT TTACGGTCAG TCCGACCAAG CAGTTCTATC ATTGCTTCGG CTGCGGTGCG CACGGCACCG CGATCGGCTT CCTGATGGAG CACGCGGGGC TCACGTTTCC CGAGGCGGTG CAGGAGCTCG CGCAGTCGGT CGGGCTCACG GTGCCGCATG AACCGTCGCC GATGCGAGGC GCGGGCGGCG GCTCGGGCGA GGGCTACGCG CCGCCCGCCG CGTCGAAATC GGTCACGACC GCGCTGTCCG ACGTGATGCA GACCGCCTGC GACTATTACC GCAAGCAGCT GCGCGGTGCG CCGAACGCGA TCCAGTATCT GAAGCGGCGC GGCCTGACGG GCGAGATCGC GCTGCGCTTC GGGCTCGGCT ACGCGCCGGA CGGCTGGCAG AACCTCGAGG CGGCGTTCGA CGATTATCGT CACGATTCGC TCGTCGACGC CGGCCTCGTG ATCGTCAGCG ATAAGACCGA CGCGAACGGC GCCGCGCGCC GCTACGACCG GTTCCGCGAG CGGATCATGT TCCCGATCCG CAACGTGAAG GGGCAGGTCA TCGGCTTTGG CGGCCGGGTG CTGGACGGCG GCGAGCCCAA GTATCTGAAT TCGCCCGAGA CGCCGCTGTT CAACAAGGGC AGCGAGTTGT ACGGCCTGTT CGAGGCGAGG CTCGCGATTC GCGAGCACAA GTACGCGCTC GTCGTCGAAG GGTACATGGA CGTCGTCGCG CTCGCGCAGC TCGGCTTCCC GAATGCGGTG GCGACCCTCG GCACCGCGTG CACGCCGGTC CACGTGCAGA AGCTGCTGCG GCAGACGGAC ACGGTCGTCT TCAGCTTCGA CGGCGATGCG GCCGGCCGGC GCGCCGCGCG GCGCGCGCTC GAGGCGTGCC TGCCGCACGC GGCGGACAAC CGGACGATCC GCTTCCTCTT CCTGCCCCCG GAGCACGATC CGGACAGCTA CGTGCGGGAA TTCGGGACGG ATGCGTTTTC GGAGCAGGTG AAGCGGGCGA TGCCGCTGTC GCAGTTCCTG CTGAACGAAG CGACGGCGGG TAAGGAGCTC GACCAGCCGG AAGGGCGCGC GAAGGCGCTG TTCGATGCGA AGCCGCTGCT GCAGGCGCTG CCCGCGAACG CGTTGCGCGC GCAGATCATG CATATGTTCG CGGACCGGCT GCGGATTCCG TTCGACGAAG TCGCCGCGCT CGCCGAGGTG GACGCGCGGA TCGCCGCGCC GGCCCGCCAG GCGCCCGCCC GCAGCGAGCG GCGGCGCGTG ACGGACAGCG AGAAGCGCGC GTTGCGCAAC CTGGTGATGC ATCCCCGTAT CGCGGCGGTG CTCGACGACG AGAGTGCTGC GACGCTGCGG GCGCTGCCGA GAATCGGGGA ACTGTTCGAC GAGGTAATCG GCCACGCGCT CGCGCTCGGA GAAGGGGCGG AATTCAGGCT TTTGTCCGAC GTGCTACGCA ATTCGGCCAA CGCGCCCACC TACGACGAAA TTTTCCGGGA AATTCTGGTC TATGATGAAA ATGTCCGCGA CTTGATGCAG CAGAATCCGG AAAGCGACGC GGCGCAGGAA GGATTGCGCG AGCGAGAACG GATCGCAGGT GAGGAGCTTC AGGCAGCGGT GCTCAAGATG CGCTATGACG CGTGCTGCGA CAGGCTCAAC AGCCTGTCGA AGCGCTCGGC GCCCTCTTCC GAGGAATTGG CGGAAATCAA GGACTTGCAT CAGAACCTGG CAGACATGAA ACGGAGGCTC GGGCTGTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA