ButhA.00150.a

Bifunctional protein glmU [Includes: UDP-N-acetylglucosamine pyrophosphorylase (EC 2.7.7.23) (N-acetylglucosamine-1-phosphate uridyltransferase); Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)] (BTH_I0289) (glmU)

CENTER ID: ButhA.00150.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00150.a.A1.GE32729 full length 1 453
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00150.a.A1.PW33333 full length 1 453

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.2949
RefSeq: YP_440847.1
UniProt: Q2T1V2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNIVILAAGT GKRMRSALPK VLHPLAGRPL LSHVIDTARA LAPSRLVVVI GHGAERVRAA VAAPDVQFAV QEQQLGTGHA VRQALPLLDP SQPTLVLYGD VPLTRAATLR RLADAATDAR YGVLTVTLDD PTGYGRIVRD QAGCVTRIVE QKDASADELK IAEINTGIVV APTAQLSMWL GALGNDNAQG EYYLTDVVEQ AIEAGFEIVT TQPDDEWETL GVNSKAQLAE LERIHQRKLA EALLADGVTL ADPARIDVRG KLTCGRDVSI DVNCVFEGDV TLADGVTIGA NCVIRNAAIA AGARVDAFSH LDGATLGANT VVGPYARLRP GAVLADDAHV GNFVEVKNAT LGHGSKANHL TYLGDADIGA RVNVGAGTIT CNYDGANKFR TVIEDDVFVG SDTQFVAPVR VGRGVTVAAG TTVWKDVAEG MLVLNDKTQT AKSGYVRPVK KKS
NT Sequence
atgaatatcg tgattttggc ggcaggcacc ggcaagcgca tgcgttcggc gctgccgaaa gtgcttcatc ctctggccgg caggcccctt ctctcccacg tgatcgacac cgcccgcgcg ctcgcgccgt cccggctcgt cgtcgtgatc ggccacggcg ccgagcgggt gcgcgcggcc gtcgccgcgc ccgacgtgca gttcgcggtg caggagcaac agctcggcac ggggcacgcg gtgcgccagg cgctgccgct gctcgacccg tcgcagccga cgctcgtgct gtacggcgac gtgccgctca cgcgcgcggc gacgctcagg cgcctcgccg acgccgcgac cgacgcccgc tacggcgtgc tgaccgtcac gctcgacgat ccgacgggct acgggcgcat cgtgcgcgac caggcggggt gcgtcacgcg catcgtcgag cagaaggacg cttcggccga cgagctgaag atcgccgaaa tcaacacggg catcgtcgtc gcgcctaccg cgcagctgtc gatgtggctc ggcgcgctcg gcaacgacaa cgcgcagggc gagtactacc tgaccgacgt cgtcgagcag gcgatcgaag cgggcttcga gatcgtcacg acgcagccgg acgacgaatg ggagacgctc ggcgtcaaca gcaaggcgca gctcgccgag ctcgagcgca ttcatcagcg caagctcgcc gaagcgctgc ttgccgatgg cgtgacgctc gccgatccgg cgcgcatcga cgtgcgcggc aagctcacgt gcgggcgcga cgtgtcgatc gacgtgaact gcgtgttcga aggcgacgtg acgcttgccg acggcgtgac gatcggcgcg aactgcgtga tccgcaacgc ggcgatcgcc gcgggcgcgc gcgtcgacgc gttctcgcat ctcgacggcg cgacgctcgg cgcgaacacg gtcgtcggcc cgtatgcacg gctgcgcccg ggcgcggtgc tcgccgacga cgcgcacgtc ggcaacttcg tcgaggtgaa gaacgcgacg ctcggccacg gctcgaaggc gaaccatctg acctatctcg gcgacgcgga catcggcgcg cgcgtgaacg tcggcgcggg cacgatcacg tgcaactacg acggcgcgaa caagttccgc acggtcatcg aggacgacgt gttcgtcggc tcggacacgc agttcgtcgc gccggtgcgc gtcggccgcg gcgtgacggt cgcggcgggc acgaccgtgt ggaaggacgt ggccgaaggc atgctcgtgc tcaacgacaa gacgcagacc gcgaaaagcg gctacgtgcg tcccgtcaag aagaagagct ga
Details for ButhA.00150.a.A1.GE32729
HARVESTED ON: 6/20/2011
SEQUENCED ON: 7/14/2011
EXPECTED MW: 49kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNIVILAAG TGKRMRSALP KVLHPLAGRP LLSHVIDTAR ALAPSRLVVV IGHGAERVRA AVAAPDVQFA VQEQQLGTGH AVRQALPLLD PSQPTLVLYG DVPLTRAATL RRLADAATDA RYGVLTVTLD DPTGYGRIVR DQAGCVTRIV EQKDASADEL KIAEINTGIV VAPTAQLSMW LGALGNDNAQ GEYYLTDVVE QAIEAGFEIV TTQPDDEWET LGVNSKAQLA ELERIHQRKL AEALLADGVT LADPARIDVR GKLTCGRDVS IDVNCVFEGD VTLADGVTIG ANCVIRNAAI AAGARVDAFS HLDGATLGAN TVVGPYARLR PGAVLADDAH VGNFVEVKNA TLGHGSKANH LTYLGDADIG ARVNVGAGTI TCNYDGANKF RTVIEDDVFV GSDTQFVAPV RVGRGVTVAA GTTVWKDVAE GMLVLNDKTQ TAKSGYVRPV KK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaata tcgtgatttt ggcggcaggc accggcaagc gcatgcgttc ggcgctgccg aaagtgcttc atcctctggc cggcaggccc cttctctccc acgtgatcga caccgcccgc gcgctcgcgc cgtcccggct cgtcgtcgtg atcggccacg gcgccgagcg ggtgcgcgcg gccgtcgccg cgcccgacgt gcagttcgcg gtgcaggagc aacagctcgg cacggggcac gcggtgcgcc aggcgctgcc gctgctcgac ccgtcgcagc cgacgctcgt gctgtacggc gacgtgccgc tcacgcgcgc ggcgacgctc aggcgcctcg ccgacgccgc gaccgacgcc cgctacggcg tgctgaccgt cacgctcgac gatccgacgg gctacgggcg catcgtgcgc gaccaggcgg ggtgcgtcac gcgcatcgtc gagcagaagg acgcttcggc cgacgagctg aagatcgccg aaatcaacac gggcatcgtc gtcgcgccta ccgcgcagct gtcgatgtgg ctcggcgcgc tcggcaacga caacgcgcag ggcgagtact acctgaccga cgtcgtcgag caggcgatcg aagcgggctt cgagatcgtc acgacgcagc cggacgacga atgggagacg ctcggcgtca acagcaaggc gcagctcgcc gagctcgagc gcattcatca gcgcaagctc gccgaagcgc tgcttgccga tggcgtgacg ctcgccgatc cggcgcgcat cgacgtgcgc ggcaagctca cgtgcgggcg cgacgtgtcg atcgacgtga actgcgtgtt cgaaggcgac gtgacgcttg ccgacggcgt gacgatcggc gcgaactgcg tgatccgcaa cgcggcgatc gccgcgggcg cgcgcgtcga cgcgttctcg catctcgacg gcgcgacgct cggcgcgaac acggtcgtcg gcccgtatgc acggctgcgc ccgggcgcgg tgctcgccga cgacgcgcac gtcggcaact tcgtcgaggt gaagaacgcg acgctcggcc acggctcgaa ggcgaaccat ctgacctatc tcggcgacgc ggacatcggc gcgcgcgtga acgtcggcgc gggcacgatc acgtgcaact acgacggcgc gaacaagttc cgcacggtca tcgaggacga cgtgttcgtc ggctcggaca cgcagttcgt cgcgccggtg cgcgtcggcc gcggcgtgac ggtcgcggcg ggcacgaccg tgtggaagga cgtggccgaa ggcatgctcg tgctcaacga caagacgcag accgcgaaaa gcggctacgt gcgtcccgtc aagaaga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNIVILAAG TGKRMRSALP KVLHPLAGRP LLSHVIDTAR ALAPSRLVVV IGHGAERVRA AVAAPDVQFA VQEQQLGTGH AVRQALPLLD PSQPTLVLYG DVPLTRAATL RRLADAATDA RYGVLTVTLD DPTGYGRIVR DQAGCVTRIV EQKDASADEL KIAEINTGIV VAPTAQLSMW LGALGNDNAQ GEYYLTDVVE QAIEAGFEIV TTQPDDEWET LGVNSKAQLA ELERIHQRKL AEALLADGVT LADPARIDVR GKLTCGRDVS IDVNCVFEGD VTLADGVTIG ANCVIRNAAI AAGARVDAFS HLDGATLGAN TVVGPYARLR PGAVLADDAH VGNFVEVKNA TLGHGSKANH LTYLGDADIG ARVNVGAGTI TCNYDGANKF RTVIEDDVFV GSDTQFVAPV RVGRGVTVAA GTTVWKDVAE GMLVLNDKTQ TAKSGYVRPV KKKS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatatcgtg attttggcgg caggcaccgg caagcgcatg cgttcggcgc tgccgaaagt gcttcatcct ctggccggca ggccccttct ctcccacgtg atcgacaccg cccgcgcgct cgcgccgtcc cggctcgtcg tcgtgatcgg ccacggcgcc gagcgggtgc gcgcggccgt cgccgcgccc gacgtgcagt tcgcggtgca ggagcaacag ctcggcacgg ggcacgcggt gcgccaggcg ctgccgctgc tcgacccgtc gcagccgacg ctcgtgctgt acggcgacgt gccgctcacg cgcgcggcga cgctcaggcg cctcgccgac gccgcgaccg acgcccgcta cggcgtgctg accgtcacgc tcgacgatcc gacgggctac gggcgcatcg tgcgcgacca ggcggggtgc gtcacgcgca tcgtcgagca gaaggacgct tcggccgacg agctgaagat cgccgaaatc aacacgggca tcgtcgtcgc gcctaccgcg cagctgtcga tgtggctcgg cgcgctcggc aacgacaacg cgcagggcga gtactacctg accgacgtcg tcgagcaggc gatcgaagcg ggcttcgaga tcgtcacgac gcagccggac gacgaatggg agacgctcgg cgtcaacagc aaggcgcagc tcgccgagct cgagcgcatt catcagcgca agctcgccga agcgctgctt gccgatggcg tgacgctcgc cgatccggcg cgcatcgacg tgcgcggcaa gctcacgtgc gggcgcgacg tgtcgatcga cgtgaactgc gtgttcgaag gcgacgtgac gcttgccgac ggcgtgacga tcggcgcgaa ctgcgtgatc cgcaacgcgg cgatcgccgc gggcgcgcgc gtcgacgcgt tctcgcatct cgacggcgcg acgctcggcg cgaacacggt cgtcggcccg tatgcacggc tgcgcccggg cgcggtgctc gccgacgacg cgcacgtcgg caacttcgtc gaggtgaaga acgcgacgct cggccacggc tcgaaggcga accatctgac ctatctcggc gacgcggaca tcggcgcgcg cgtgaacgtc ggcgcgggca cgatcacgtg caactacgac ggcgcgaaca agttccgcac ggtcatcgag gacgacgtgt tcgtcggctc ggacacgcag ttcgtcgcgc cggtgcgcgt cggccgcggc gtgacggtcg cggcgggcac gaccgtgtgg aaggacgtgg ccgaaggcat gctcgtgctc aacgacaaga cgcagaccgc gaaaagcggc tacgtgcgtc ccgtcaagaa gaagagctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for ButhA.00150.a.A1.PW33333
PURIFICATION DATe: 8/19/2011
CONCENTRATION: 30.77mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNIVILAAG TGKRMRSALP KVLHPLAGRP LLSHVIDTAR ALAPSRLVVV IGHGAERVRA AVAAPDVQFA VQEQQLGTGH AVRQALPLLD PSQPTLVLYG DVPLTRAATL RRLADAATDA RYGVLTVTLD DPTGYGRIVR DQAGCVTRIV EQKDASADEL KIAEINTGIV VAPTAQLSMW LGALGNDNAQ GEYYLTDVVE QAIEAGFEIV TTQPDDEWET LGVNSKAQLA ELERIHQRKL AEALLADGVT LADPARIDVR GKLTCGRDVS IDVNCVFEGD VTLADGVTIG ANCVIRNAAI AAGARVDAFS HLDGATLGAN TVVGPYARLR PGAVLADDAH VGNFVEVKNA TLGHGSKANH LTYLGDADIG ARVNVGAGTI TCNYDGANKF RTVIEDDVFV GSDTQFVAPV RVGRGVTVAA GTTVWKDVAE GMLVLNDKTQ TAKSGYVRPV KK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaata tcgtgatttt ggcggcaggc accggcaagc gcatgcgttc ggcgctgccg aaagtgcttc atcctctggc cggcaggccc cttctctccc acgtgatcga caccgcccgc gcgctcgcgc cgtcccggct cgtcgtcgtg atcggccacg gcgccgagcg ggtgcgcgcg gccgtcgccg cgcccgacgt gcagttcgcg gtgcaggagc aacagctcgg cacggggcac gcggtgcgcc aggcgctgcc gctgctcgac ccgtcgcagc cgacgctcgt gctgtacggc gacgtgccgc tcacgcgcgc ggcgacgctc aggcgcctcg ccgacgccgc gaccgacgcc cgctacggcg tgctgaccgt cacgctcgac gatccgacgg gctacgggcg catcgtgcgc gaccaggcgg ggtgcgtcac gcgcatcgtc gagcagaagg acgcttcggc cgacgagctg aagatcgccg aaatcaacac gggcatcgtc gtcgcgccta ccgcgcagct gtcgatgtgg ctcggcgcgc tcggcaacga caacgcgcag ggcgagtact acctgaccga cgtcgtcgag caggcgatcg aagcgggctt cgagatcgtc acgacgcagc cggacgacga atgggagacg ctcggcgtca acagcaaggc gcagctcgcc gagctcgagc gcattcatca gcgcaagctc gccgaagcgc tgcttgccga tggcgtgacg ctcgccgatc cggcgcgcat cgacgtgcgc ggcaagctca cgtgcgggcg cgacgtgtcg atcgacgtga actgcgtgtt cgaaggcgac gtgacgcttg ccgacggcgt gacgatcggc gcgaactgcg tgatccgcaa cgcggcgatc gccgcgggcg cgcgcgtcga cgcgttctcg catctcgacg gcgcgacgct cggcgcgaac acggtcgtcg gcccgtatgc acggctgcgc ccgggcgcgg tgctcgccga cgacgcgcac gtcggcaact tcgtcgaggt gaagaacgcg acgctcggcc acggctcgaa ggcgaaccat ctgacctatc tcggcgacgc ggacatcggc gcgcgcgtga acgtcggcgc gggcacgatc acgtgcaact acgacggcgc gaacaagttc cgcacggtca tcgaggacga cgtgttcgtc ggctcggaca cgcagttcgt cgcgccggtg cgcgtcggcc gcggcgtgac ggtcgcggcg ggcacgaccg tgtggaagga cgtggccgaa ggcatgctcg tgctcaacga caagacgcag accgcgaaaa gcggctacgt gcgtcccgtc aagaaga
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNIVILAAG TGKRMRSALP KVLHPLAGRP LLSHVIDTAR ALAPSRLVVV IGHGAERVRA AVAAPDVQFA VQEQQLGTGH AVRQALPLLD PSQPTLVLYG DVPLTRAATL RRLADAATDA RYGVLTVTLD DPTGYGRIVR DQAGCVTRIV EQKDASADEL KIAEINTGIV VAPTAQLSMW LGALGNDNAQ GEYYLTDVVE QAIEAGFEIV TTQPDDEWET LGVNSKAQLA ELERIHQRKL AEALLADGVT LADPARIDVR GKLTCGRDVS IDVNCVFEGD VTLADGVTIG ANCVIRNAAI AAGARVDAFS HLDGATLGAN TVVGPYARLR PGAVLADDAH VGNFVEVKNA TLGHGSKANH LTYLGDADIG ARVNVGAGTI TCNYDGANKF RTVIEDDVFV GSDTQFVAPV RVGRGVTVAA GTTVWKDVAE GMLVLNDKTQ TAKSGYVRPV KKKS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAATATCGTG ATTTTGGCGG CAGGCACCGG CAAGCGCATG CGTTCGGCGC TGCCGAAAGT GCTTCATCCT CTGGCCGGCA GGCCCCTTCT CTCCCACGTG ATCGACACCG CCCGCGCGCT CGCGCCGTCC CGGCTCGTCG TCGTGATCGG CCACGGCGCC GAGCGGGTGC GCGCGGCCGT CGCCGCGCCC GACGTGCAGT TCGCGGTGCA GGAGCAACAG CTCGGCACGG GGCACGCGGT GCGCCAGGCG CTGCCGCTGC TCGACCCGTC GCAGCCGACG CTCGTGCTGT ACGGCGACGT GCCGCTCACG CGCGCGGCGA CGCTCAGGCG CCTCGCCGAC GCCGCGACCG ACGCCCGCTA CGGCGTGCTG ACCGTCACGC TCGACGATCC GACGGGCTAC GGGCGCATCG TGCGCGACCA GGCGGGGTGC GTCACGCGCA TCGTCGAGCA GAAGGACGCT TCGGCCGACG AGCTGAAGAT CGCCGAAATC AACACGGGCA TCGTCGTCGC GCCTACCGCG CAGCTGTCGA TGTGGCTCGG CGCGCTCGGC AACGACAACG CGCAGGGCGA GTACTACCTG ACCGACGTCG TCGAGCAGGC GATCGAAGCG GGCTTCGAGA TCGTCACGAC GCAGCCGGAC GACGAATGGG AGACGCTCGG CGTCAACAGC AAGGCGCAGC TCGCCGAGCT CGAGCGCATT CATCAGCGCA AGCTCGCCGA AGCGCTGCTT GCCGATGGCG TGACGCTCGC CGATCCGGCG CGCATCGACG TGCGCGGCAA GCTCACGTGC GGGCGCGACG TGTCGATCGA CGTGAACTGC GTGTTCGAAG GCGACGTGAC GCTTGCCGAC GGCGTGACGA TCGGCGCGAA CTGCGTGATC CGCAACGCGG CGATCGCCGC GGGCGCGCGC GTCGACGCGT TCTCGCATCT CGACGGCGCG ACGCTCGGCG CGAACACGGT CGTCGGCCCG TATGCACGGC TGCGCCCGGG CGCGGTGCTC GCCGACGACG CGCACGTCGG CAACTTCGTC GAGGTGAAGA ACGCGACGCT CGGCCACGGC TCGAAGGCGA ACCATCTGAC CTATCTCGGC GACGCGGACA TCGGCGCGCG CGTGAACGTC GGCGCGGGCA CGATCACGTG CAACTACGAC GGCGCGAACA AGTTCCGCAC GGTCATCGAG GACGACGTGT TCGTCGGCTC GGACACGCAG TTCGTCGCGC CGGTGCGCGT CGGCCGCGGC GTGACGGTCG CGGCGGGCAC GACCGTGTGG AAGGACGTGG CCGAAGGCAT GCTCGTGCTC AACGACAAGA CGCAGACCGC GAAAAGCGGC TACGTGCGTC CCGTCAAGAA GAAGAGCTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA