ButhA.00149.a

Methoxy mycolic acid synthase 2

CENTER ID: ButhA.00149.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00149.a.B1.GE36122 Full length( ButhA.00149.a ) 1 311
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00149.a.B1.PS01694 Full length( ButhA.00149.a ) 1 311

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.314
RefSeq: YP_438481.1
UniProt: Q2T8L8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTVHLESRES VAPNRGAPYG ASADAIQYHY DIGNAFLALA QERGRNYSCA MYEEGDTHEQ AQIRKLDYHI AQIRAHGAAR VLDIGCGWGA LLDRLVTVAG VKQAVGLTLS NEQIRYIGEQ YPHPNVDVLL RNWQDYEPEQ PFDGIISLGA FEHFAKIDED KVQAYRHFFR KCHDFLKPGG RLSLQTMGYG DVPRERRHSD LFIAREVFPE SDLPYLADIV RACEMLFEVE LVRNDRHDYV KTMRAWFENL RAHRREALEL APLDVVERYE RLYRTMSYSF DLGAFVLYRI TFRRIEPNRI DAGARHRAAL A
NT Sequence
ATGACCGTCC ATCTCGAAAG CCGCGAATCC GTCGCGCCGA ATCGCGGCGC GCCGTACGGC GCGTCCGCCG ACGCGATCCA GTACCACTAC GACATCGGCA ATGCCTTCCT CGCGCTCGCG CAGGAACGCG GCCGCAACTA CTCGTGCGCG ATGTACGAGG AAGGCGATAC GCACGAGCAG GCGCAGATCC GCAAGCTCGA CTATCACATC GCGCAGATCC GTGCGCACGG TGCGGCGCGC GTGCTCGATA TCGGCTGCGG CTGGGGCGCG CTGCTCGACC GGCTCGTGAC GGTCGCCGGC GTGAAGCAGG CCGTGGGCCT CACGCTGTCG AACGAGCAGA TCCGCTACAT CGGCGAGCAA TACCCGCATC CGAACGTCGA CGTGCTGCTG CGCAACTGGC AGGACTACGA GCCCGAGCAG CCGTTCGACG GCATCATCTC GCTCGGCGCG TTCGAGCACT TCGCGAAGAT CGACGAAGAC AAGGTGCAGG CGTACCGGCA CTTCTTCAGG AAATGCCATG ACTTCCTGAA ACCGGGCGGG CGCCTTTCGC TGCAGACGAT GGGCTACGGC GACGTGCCGC GCGAGCGCCG GCATTCGGAC CTCTTCATCG CGCGCGAAGT CTTTCCGGAA TCGGATCTGC CGTATCTGGC CGATATCGTG CGCGCCTGCG AGATGCTGTT CGAAGTCGAA CTCGTGCGCA ACGATCGCCA CGATTACGTG AAGACGATGC GCGCGTGGTT CGAGAACCTG CGCGCGCATC GCCGCGAGGC GCTCGAGCTC GCGCCGCTCG ACGTGGTCGA GCGCTACGAG CGGCTTTACC GGACGATGAG CTATTCGTTC GATCTCGGCG CGTTCGTGCT GTATCGAATC ACGTTCCGGC GCATCGAGCC GAACCGGATC GACGCCGGCG CGCGGCATCG GGCGGCGCTC GCA
Details for ButhA.00149.a.B1.GE36122
HARVESTED ON: 9/26/2012
SEQUENCED ON: 9/25/2012
EXPECTED MW: 36kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MTVHLESRES VAPNRGAPYG ASADAIQYHY DIGNAFLALA QERGRNYSCA MYEEGDTHEQ AQIRKLDYHI AQIRAHGAAR VLDIGCGWGA LLDRLVTVAG VKQAVGLTLS NEQIRYIGEQ YPHPNVDVLL RNWQDYEPEQ PFDGIISLGA FEHFAKIDED KVQAYRHFFR KCHDFLKPGG RLSLQTMGYG DVPRERRHSD LFIAREVFPE SDLPYLADIV RACEMLFEVE LVRNDRHDYV KTMRAWFENL RAHRREALEL APLDVVERYE RLYRTMSYSF DLGAFVLYRI TFRRIEPNRI DAGARHRAAL A
Validated NT Sequence
tactnnctta tgcgagcgcc gcccgatgcc gcgcgccggc gtcgatccgg ttcggctcga tgcgccggaa cgtgattcga tacagcacga acgcgccgag atcgaacgaa tagctcatcg tccggtaaag ccgctcgtag cgctcgacca cgtcgagcgg cgcgagctcg agcgcctcgc ggcgatgcgc gcgcaggttc tcgaaccacg cgcgcatcgt cttcacgtaa tcgtggcgat cgttgcgcac gagttcgact tcgaacagca tctcgcaggc gcgcacgata tcggccagat acggcagatc cgattccgga aagacttcgc gcgcgatgaa gaggtccgaa tgccggcgct cgcgcggcac gtcgccgtag cccatcgtct gcagcgaaag gcgcccgccc ggtttcagga agtcatggca tttcctgaag aagtgccggt acgcctgcac cttgtcttcg tcgatcttcg cgaagtgctc gaacgcgccg agcgagatga tgccgtcgaa cggctgctcg ggctcgtagt cctgccagtt gcgcagcagc acgtcgacgt tcggatgcgg gtattgctcg ccgatgtagc ggatctgctc gttcgacagc gtgaggccca cggcctgctt cacgccggcg accgtcacga gccggtcgag cagcgcgccc cagccgcagc cgatatcgag cacgcgcgcc gcaccgtgcg cacggatctg cgcgatgtga tagtcgagct tgcggatctg cgcctgctcg tgcgtatcgc cttcctcgta catcgcgcac gagtagttgc ggccgcgttc ctgcgcgagc gcgaggaagg cattgccgat gtcgtagtgg tactggatcg cgtcggcgga cgcgccgtac ggcgcgccgc gattcggcgc gacggattcg cggctttcga gatggacggt catatggtgg tggtgg
Expected Protein Sequence
MAHHHHHHMT VHLESRESVA PNRGAPYGAS ADAIQYHYDI GNAFLALAQE RGRNYSCAMY EEGDTHEQAQ IRKLDYHIAQ IRAHGAARVL DIGCGWGALL DRLVTVAGVK QAVGLTLSNE QIRYIGEQYP HPNVDVLLRN WQDYEPEQPF DGIISLGAFE HFAKIDEDKV QAYRHFFRKC HDFLKPGGRL SLQTMGYGDV PRERRHSDLF IAREVFPESD LPYLADIVRA CEMLFEVELV RNDRHDYVKT MRAWFENLRA HRREALELAP LDVVERYERL YRTMSYSFDL GAFVLYRITF RRIEPNRIDA GARHRAALA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg tccatctcga aagccgcgaa tccgtcgcgc cgaatcgcgg cgcgccgtac ggcgcgtccg ccgacgcgat ccagtaccac tacgacatcg gcaatgcctt cctcgcgctc gcgcaggaac gcggccgcaa ctactcgtgc gcgatgtacg aggaaggcga tacgcacgag caggcgcaga tccgcaagct cgactatcac atcgcgcaga tccgtgcgca cggtgcggcg cgcgtgctcg atatcggctg cggctggggc gcgctgctcg accggctcgt gacggtcgcc ggcgtgaagc aggccgtggg cctcacgctg tcgaacgagc agatccgcta catcggcgag caatacccgc atccgaacgt cgacgtgctg ctgcgcaact ggcaggacta cgagcccgag cagccgttcg acggcatcat ctcgctcggc gcgttcgagc acttcgcgaa gatcgacgaa gacaaggtgc aggcgtaccg gcacttcttc aggaaatgcc atgacttcct gaaaccgggc gggcgccttt cgctgcagac gatgggctac ggcgacgtgc cgcgcgagcg ccggcattcg gacctcttca tcgcgcgcga agtctttccg gaatcggatc tgccgtatct ggccgatatc gtgcgcgcct gcgagatgct gttcgaagtc gaactcgtgc gcaacgatcg ccacgattac gtgaagacga tgcgcgcgtg gttcgagaac ctgcgcgcgc atcgccgcga ggcgctcgag ctcgcgccgc tcgacgtggt cgagcgctac gagcggcttt accggacgat gagctattcg ttcgatctcg gcgcgttcgt gctgtatcga atcacgttcc ggcgcatcga gccgaaccgg atcgacgccg gcgcgcggca tcgggcggcg ctcgcatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for ButhA.00149.a.B1.PS01694
PURIFICATION DATe: 11/29/2012
CONCENTRATION: 8.6mg/ml
OBSERVED MW: 35kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MTVHLESRES VAPNRGAPYG ASADAIQYHY DIGNAFLALA QERGRNYSCA MYEEGDTHEQ AQIRKLDYHI AQIRAHGAAR VLDIGCGWGA LLDRLVTVAG VKQAVGLTLS NEQIRYIGEQ YPHPNVDVLL RNWQDYEPEQ PFDGIISLGA FEHFAKIDED KVQAYRHFFR KCHDFLKPGG RLSLQTMGYG DVPRERRHSD LFIAREVFPE SDLPYLADIV RACEMLFEVE LVRNDRHDYV KTMRAWFENL RAHRREALEL APLDVVERYE RLYRTMSYSF DLGAFVLYRI TFRRIEPNRI DAGARHRAAL A
Validated NT Sequence
tactnnctta tgcgagcgcc gcccgatgcc gcgcgccggc gtcgatccgg ttcggctcga tgcgccggaa cgtgattcga tacagcacga acgcgccgag atcgaacgaa tagctcatcg tccggtaaag ccgctcgtag cgctcgacca cgtcgagcgg cgcgagctcg agcgcctcgc ggcgatgcgc gcgcaggttc tcgaaccacg cgcgcatcgt cttcacgtaa tcgtggcgat cgttgcgcac gagttcgact tcgaacagca tctcgcaggc gcgcacgata tcggccagat acggcagatc cgattccgga aagacttcgc gcgcgatgaa gaggtccgaa tgccggcgct cgcgcggcac gtcgccgtag cccatcgtct gcagcgaaag gcgcccgccc ggtttcagga agtcatggca tttcctgaag aagtgccggt acgcctgcac cttgtcttcg tcgatcttcg cgaagtgctc gaacgcgccg agcgagatga tgccgtcgaa cggctgctcg ggctcgtagt cctgccagtt gcgcagcagc acgtcgacgt tcggatgcgg gtattgctcg ccgatgtagc ggatctgctc gttcgacagc gtgaggccca cggcctgctt cacgccggcg accgtcacga gccggtcgag cagcgcgccc cagccgcagc cgatatcgag cacgcgcgcc gcaccgtgcg cacggatctg cgcgatgtga tagtcgagct tgcggatctg cgcctgctcg tgcgtatcgc cttcctcgta catcgcgcac gagtagttgc ggccgcgttc ctgcgcgagc gcgaggaagg cattgccgat gtcgtagtgg tactggatcg cgtcggcgga cgcgccgtac ggcgcgccgc gattcggcgc gacggattcg cggctttcga gatggacggt catatggtgg tggtgg
Expressed Protein Sequence
MAHHHHHHMT VHLESRESVA PNRGAPYGAS ADAIQYHYDI GNAFLALAQE RGRNYSCAMY EEGDTHEQAQ IRKLDYHIAQ IRAHGAARVL DIGCGWGALL DRLVTVAGVK QAVGLTLSNE QIRYIGEQYP HPNVDVLLRN WQDYEPEQPF DGIISLGAFE HFAKIDEDKV QAYRHFFRKC HDFLKPGGRL SLQTMGYGDV PRERRHSDLF IAREVFPESD LPYLADIVRA CEMLFEVELV RNDRHDYVKT MRAWFENLRA HRREALELAP LDVVERYERL YRTMSYSFDL GAFVLYRITF RRIEPNRIDA GARHRAALA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCG TCCATCTCGA AAGCCGCGAA TCCGTCGCGC CGAATCGCGG CGCGCCGTAC GGCGCGTCCG CCGACGCGAT CCAGTACCAC TACGACATCG GCAATGCCTT CCTCGCGCTC GCGCAGGAAC GCGGCCGCAA CTACTCGTGC GCGATGTACG AGGAAGGCGA TACGCACGAG CAGGCGCAGA TCCGCAAGCT CGACTATCAC ATCGCGCAGA TCCGTGCGCA CGGTGCGGCG CGCGTGCTCG ATATCGGCTG CGGCTGGGGC GCGCTGCTCG ACCGGCTCGT GACGGTCGCC GGCGTGAAGC AGGCCGTGGG CCTCACGCTG TCGAACGAGC AGATCCGCTA CATCGGCGAG CAATACCCGC ATCCGAACGT CGACGTGCTG CTGCGCAACT GGCAGGACTA CGAGCCCGAG CAGCCGTTCG ACGGCATCAT CTCGCTCGGC GCGTTCGAGC ACTTCGCGAA GATCGACGAA GACAAGGTGC AGGCGTACCG GCACTTCTTC AGGAAATGCC ATGACTTCCT GAAACCGGGC GGGCGCCTTT CGCTGCAGAC GATGGGCTAC GGCGACGTGC CGCGCGAGCG CCGGCATTCG GACCTCTTCA TCGCGCGCGA AGTCTTTCCG GAATCGGATC TGCCGTATCT GGCCGATATC GTGCGCGCCT GCGAGATGCT GTTCGAAGTC GAACTCGTGC GCAACGATCG CCACGATTAC GTGAAGACGA TGCGCGCGTG GTTCGAGAAC CTGCGCGCGC ATCGCCGCGA GGCGCTCGAG CTCGCGCCGC TCGACGTGGT CGAGCGCTAC GAGCGGCTTT ACCGGACGAT GAGCTATTCG TTCGATCTCG GCGCGTTCGT GCTGTATCGA ATCACGTTCC GGCGCATCGA GCCGAACCGG ATCGACGCCG GCGCGCGGCA TCGGGCGGCG CTCGCATGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T