SSGCID: Target Page

ButhA.00148.b

Site-specific recombinase, phage integrase family

CENTER ID:	ButhA.00148.b
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00148.b.B1.GE36119	Full length( ButhA.00148.b )		1	222

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
RefSeq:	YP_443627.1
UniProt:	Q2STX2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVAAWRDARL GEVSSGTALR ELQLLGHVFS VAIREWGAQL PSNPVSLIRK PRSGKARDRV LTTSERTALI SACSQCHNPW IKPVVIFALE TAARRGEILA LTWQDVDLAR RTATLQITKA GQPRRIPLSA TCVTMLEALP RSLDGRVFPV SMETVKQAYE RAVARAGITN FTFHDLRHDA LTRLAKQGLS ILELRAISGH ATANMLQRYV SIDAGELAMK LG

NT Sequence

ATGGTCGCGG CGTGGCGGGA TGCCCGACTT GGAGAAGTTT CGTCCGGCAC CGCACTGCGG GAGCTTCAGC TTCTTGGACA TGTCTTCAGC GTAGCGATTC GCGAGTGGGG CGCTCAGCTT CCCAGCAATC CCGTTAGCCT TATCAGGAAG CCACGGTCTG GCAAGGCCCG AGATCGCGTG CTGACCACAT CGGAAAGGAC CGCGCTCATC TCGGCTTGCA GTCAGTGTCA CAACCCGTGG ATTAAGCCTG TCGTGATCTT CGCGCTGGAA ACCGCTGCAC GACGCGGCGA AATCCTTGCA CTGACATGGC AAGACGTTGA TTTGGCACGT CGGACGGCGA CCCTACAGAT TACGAAGGCA GGGCAACCGC GAAGGATTCC GCTATCCGCA ACGTGTGTCA CCATGCTTGA AGCACTACCG CGCAGCCTTG ACGGACGGGT CTTCCCTGTT TCGATGGAAA CAGTCAAGCA GGCATACGAA CGAGCGGTTG CACGGGCCGG TATCACCAAC TTCACTTTCC ATGATCTGAG ACACGATGCA CTGACGCGAC TGGCAAAGCA GGGGCTTTCC ATTCTGGAAC TTCGGGCAAT ATCCGGTCAC GCAACCGCGA ACATGCTTCA GCGATACGTG TCGATTGACG CCGGTGAATT GGCAATGAAG CTCGGC

Details for ButhA.00148.b.B1.GE36119

HARVESTED ON:	9/26/2012
SEQUENCED ON:	9/25/2012
EXPECTED MW:	25kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV AAWRDARLGE VSSGTALREL QLLGHVFSVA IREWGAQLPS NPVSLIRKPR SGKARDRVLT TSERTALISA CSQCHNPWIK PVVIFALETA ARRGEILALT WQDVDLARRT ATLQITKAGQ PRRIPLSATC VTMLEALPRS LDGRVFPVSM ETVKQAYERA VARAGITNFT FHDLRHDALT RLAKQGLSIL ELRAISGHAT ANMLQRYVSI DAXELAMKLG

Validated NT Sequence

ttnnnnnnnt taagaaggag anntaccatg gctcaccacc accaccacca tatggtcgcg gcgtggcggg atgcccgact tggagaagtt tcgtccggca ccgcactgcg ggagcttcag cttcttggac atgtcttcag cgtagcgatt cgcgagtggg gcgctcagct tcccagcaat cccgttagcc ttatcaggaa gccacggtct ggcaaggccc gagatcgcgt gctgaccaca tcggaaagga ccgcgctcat ctcggcttgc agtcagtgtc acaacccgtg gattaagcct gtcgtgatct tcgcgctgga aaccgctgca cgacgcggcg aaatccttgc actgacatgg caagacgttg atttggcacg tcggacggcg accctacaga ttacgaaggc agggcaaccg cgaaggattc cgctatccgc aacgtgtgtc accatgcttg aagcactacc gcgcagcctt gacggacggg tcttccctgt ttcgatggaa acagtcaagc aggcatacga acgagcggtt gcacgggccg gtatcaccaa cttcactttc catgatctga gacacgatgc actgacgcga ctggcaaagc aggggctttc cattctggaa cttcgggcaa tatccggtca cgcaaccgcg aacatgcttc agcgatacgt gtcgattgac gccngtgaat tggcaatgaa gctcggctaa gtgagtaaga taggatccgg ctgcnnnnnn ccccnnnccn gnncnnnnng nnnnnnnntn nnnnnnnnnn gnnnnctnnn nnnnannna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtcg cggcgtggcg ggatgcccga cttggagaag tttcgtccgg caccgcactg cgggagcttc agcttcttgg acatgtcttc agcgtagcga ttcgcgagtg gggcgctcag cttcccagca atcccgttag ccttatcagg aagccacggt ctggcaaggc ccgagatcgc gtgctgacca catcggaaag gaccgcgctc atctcggctt gcagtcagtg tcacaacccg tggattaagc ctgtcgtgat cttcgcgctg gaaaccgctg cacgacgcgg cgaaatcctt gcactgacat ggcaagacgt tgatttggca cgtcggacgg cgaccctaca gattacgaag gcagggcaac cgcgaaggat tccgctatcc gcaacgtgtg tcaccatgct tgaagcacta ccgcgcagcc ttgacggacg ggtcttccct gtttcgatgg aaacagtcaa gcaggcatac gaacgagcgg ttgcacgggc cggtatcacc aacttcactt tccatgatct gagacacgat gcactgacgc gactggcaaa gcaggggctt tccattctgg aacttcgggc aatatccggt cacgcaaccg cgaacatgct tcagcgatac gtgtcgattg acgccggtga attggcaatg aagctcggct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat