ButhA.00137.b

UDP-N-acetylmuramate--L-alanine ligase (EC 6.3.2.8) (UDP-N-acetylmuramoyl-L-alanine synthetase) (BTH_I1119) (murC)

CENTER ID: ButhA.00137.b
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00137.b.A1.GE32725 full length 1 465
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00137.b.A1.PW33329 full length 1 465

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.3875
RefSeq: YP_441667.1
UniProt: Q2SZI2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKHIVKHIHF VGIGGAGMSG IAEVLVNLGY QVSGSDLARN AVTERLEALG ARVSIGHDAA NIEGANAVVV STAVRSDNPE VLAARRLRVP IVPRAVMLAE LMRLKQGIAI AGTHGKTTTT SLVASVLAAG GLDPTFVIGG RLTSAGANAR LGTGDFIVAE ADESDASFLN LYPVIEVITN IDADHMDTYG HDFARLKQAF IEFTQRLPFY GSAVVCIDDP NVRQIVPLIS KPVVRYGFAA DAQVRAENVE ARDGRMHFTV LREGREPLPV VLNLPGLHNV QNALAAIAIA TDLDVADTAI QQALAEFNGV GRRFQRYGEM PAAGGGAYTL IDDYGHHPVE MAATIAAARG AFPGRRLVLA FQPHRYTRTR DCFDDFVNVL STVDALVLTE VYAAGEAPIS TANGDALSRA LRAAGKVEPV FVATVDEVPD ALAKLARAGD VVITMGAGSI GGVPGKLAQD TQQKG
NT Sequence
atgaaacaca tcgtcaaaca cattcatttc gtcgggatcg gcggcgcggg catgagcggc atcgccgaag tgctcgtgaa cctgggctat caggtgagcg gctccgatct cgcgcgcaac gcggtgaccg agcgcctcga ggcgctcggc gcgcgcgtgt cgatcggcca cgacgcggcg aacatcgagg gcgcgaacgc ggtggtcgtg tcgaccgcgg tgcgctcgga caacccggag gtgctcgccg cgcgccgcct gcgcgtgccg atcgtgccgc gcgcggtgat gctcgcggag ctgatgcgcc tgaagcaggg gatcgcgatc gccggcacgc acggcaagac gacgacgacg agcctcgtcg cgagcgtgct cgccgcgggc gggctcgatc cgacgttcgt gatcggcggg cgtctcacga gcgccggggc gaacgcgcgg ctcggcacgg gcgacttcat cgtcgcggaa gccgacgagt cggacgcgtc gttcctgaac ctgtatccgg tgatcgaggt catcacgaac atcgacgccg atcacatgga cacctacggc cacgatttcg cgcggctcaa gcaggcgttc atcgaattca cgcagcggct gccgttctac ggcagcgccg tcgtgtgcat cgacgacccg aacgtgcggc agatcgtgcc gctgatctcg aagcccgtcg tgcgctacgg cttcgcggcg gatgcgcagg tgcgcgcgga gaacgtcgag gcgcgcgacg gccggatgca cttcacggtg ctgcgcgagg ggcgcgagcc gctgcccgtc gtcctgaacc tgccgggcct gcacaacgtg cagaacgcgc tcgccgcgat cgcgatcgcg accgatctcg acgtggccga cacggcgatt cagcaggcgc tcgcggaatt caacggcgtg ggccggcgct tccagcgcta cggggagatg ccggccgcgg gcggcggcgc gtacacgctg atcgacgact acggccacca tccggtcgag atggcggcga cgatcgcggc ggcgcgcggc gcgttcccgg gccgccggct cgtgctcgct ttccagccgc atcgctacac gcgcacgcgc gactgcttcg acgatttcgt caacgtgctg tcgacggtcg acgcgctggt gctgaccgag gtgtacgcgg ccggcgaggc gccgatttcg accgcgaacg gcgatgcgct gtcgcgcgcg ctgcgcgcgg cgggcaaggt cgagccggta ttcgtcgcga cggtcgacga ggtgccggac gcgctcgcga agctcgcgcg cgccggcgac gtggtgatca cgatgggcgc gggctcgatc ggcggggtgc cgggcaagct cgcgcaggac acgcaacaga agggatga
Details for ButhA.00137.b.A1.GE32725
HARVESTED ON: 6/20/2011
SEQUENCED ON: 7/19/2011
EXPECTED MW: 51kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKHIVKHIH FVGIGGAGMS GIAEVLVNLG YQVSGSDLAR NAVTERLEAL GARVSIGHDA ANIEGANAVV VSTAVRSDNP EVLAARRLRV PIVPRAVMLA ELMRLKQGIA IAGTHGKTTT TSLVASVLAA GGLDPTFVIG GRLTSAGANA RLGTGDFIVA EADESDASFL NLYPVIEVIT NIDADHMDTY GHDFARLKQA FIEFTQRLPF YGSAVVCIDD PNVRQIVPLI SKPVVRYGFA ADAQVRAENV EARDGRMHFT VLREGREPLP VVLNLPGLHN VQNALAAIAI ATDLDVADTA IQQALAEFNG VGRRFQRYGE MPAAGGGAYT LIDDYGHHPV EMAATIAAAR GAFPGRRLVL AFQPHRYTRT RDCFDDFVNV LSTVDALVLT EVYAAGEAPI STANGDALSR ALRAAGKVEP VFVATVDEVP DALAKLARAG DVVITMGAGS IGGVPGKLAQ DTQQ
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaac acatcgtcaa acacattcat ttcgtcggga tcggcggcgc gggcatgagc ggcatcgccg aagtgctcgt gaacctgggc tatcaggtga gcggctccga tctcgcgcgc aacgcggtga ccgagcgcct cgaggcgctc ggcgcgcgcg tgtcgatcgg ccacgacgcg gcgaacatcg agggcgcgaa cgcggtggtc gtgtcgaccg cggtgcgctc ggacaacccg gaggtgctcg ccgcgcgccg cctgcgcgtg ccgatcgtgc cgcgcgcggt gatgctcgcg gagctgatgc gcctgaagca ggggatcgcg atcgccggca cgcacggcaa gacgacgacg acgagcctcg tcgcgagcgt gctcgccgcg ggcgggctcg atccgacgtt cgtgatcggc gggcgtctca cgagcgccgg ggcgaacgcg cggctcggca cgggcgactt catcgtcgcg gaagccgacg agtcggacgc gtcgttcctg aacctgtatc cggtgatcga ggtcatcacg aacatcgacg ccgatcacat ggacacctac ggccacgatt tcgcgcggct caagcaggcg ttcatcgaat tcacgcagcg gctgccgttc tacggcagcg ccgtcgtgtg catcgacgac ccgaacgtgc ggcagatcgt gccgctgatc tcgaagcccg tcgtgcgcta cggcttcgcg gcggatgcgc aggtgcgcgc ggagaacgtc gaggcgcgcg acggccggat gcacttcacg gtgctgcgcg aggggcgcga gccgctgccc gtcgtcctga acctgccggg cctgcacaac gtgcagaacg cgctcgccgc gatcgcgatc gcgaccgatc tcgacgtggc cgacacggcg attcagcagg cgctcgcgga attcaacggc gtgggccggc gcttccagcg ctacggggag atgccggccg cgggcggcgg cgcgtacacg ctgatcgacg actacggcca ccatccggtc gagatggcgg cgacgatcgc ggcggcgcgc ggcgcgttcc cgggccgccg gctcgtgctc gctttccagc cgcatcgcta cacgcgcacg cgcgactgct tcgacgattt cgtcaacgtg ctgtcgacgg tcgacgcgct ggtgctgacc gaggtgtacg cggccggcga ggcgccgatt tcgaccgcga acggcgatgc gctgtcgcgc gcgctgcgcg cggcgggcaa ggtcgagccg gtattcgtcg cgacggtcga cgaggtgccg gacgcgctcg cgaagctcgc gcgcgccggc gacgtggtga tcacgatggg cgcgggctcg atcggcgggg tgccgggcaa gctcgcgcag gacacgcaac aga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKHIVKHIH FVGIGGAGMS GIAEVLVNLG YQVSGSDLAR NAVTERLEAL GARVSIGHDA ANIEGANAVV VSTAVRSDNP EVLAARRLRV PIVPRAVMLA ELMRLKQGIA IAGTHGKTTT TSLVASVLAA GGLDPTFVIG GRLTSAGANA RLGTGDFIVA EADESDASFL NLYPVIEVIT NIDADHMDTY GHDFARLKQA FIEFTQRLPF YGSAVVCIDD PNVRQIVPLI SKPVVRYGFA ADAQVRAENV EARDGRMHFT VLREGREPLP VVLNLPGLHN VQNALAAIAI ATDLDVADTA IQQALAEFNG VGRRFQRYGE MPAAGGGAYT LIDDYGHHPV EMAATIAAAR GAFPGRRLVL AFQPHRYTRT RDCFDDFVNV LSTVDALVLT EVYAAGEAPI STANGDALSR ALRAAGKVEP VFVATVDEVP DALAKLARAG DVVITMGAGS IGGVPGKLAQ DTQQKG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaacacatc gtcaaacaca ttcatttcgt cgggatcggc ggcgcgggca tgagcggcat cgccgaagtg ctcgtgaacc tgggctatca ggtgagcggc tccgatctcg cgcgcaacgc ggtgaccgag cgcctcgagg cgctcggcgc gcgcgtgtcg atcggccacg acgcggcgaa catcgagggc gcgaacgcgg tggtcgtgtc gaccgcggtg cgctcggaca acccggaggt gctcgccgcg cgccgcctgc gcgtgccgat cgtgccgcgc gcggtgatgc tcgcggagct gatgcgcctg aagcagggga tcgcgatcgc cggcacgcac ggcaagacga cgacgacgag cctcgtcgcg agcgtgctcg ccgcgggcgg gctcgatccg acgttcgtga tcggcgggcg tctcacgagc gccggggcga acgcgcggct cggcacgggc gacttcatcg tcgcggaagc cgacgagtcg gacgcgtcgt tcctgaacct gtatccggtg atcgaggtca tcacgaacat cgacgccgat cacatggaca cctacggcca cgatttcgcg cggctcaagc aggcgttcat cgaattcacg cagcggctgc cgttctacgg cagcgccgtc gtgtgcatcg acgacccgaa cgtgcggcag atcgtgccgc tgatctcgaa gcccgtcgtg cgctacggct tcgcggcgga tgcgcaggtg cgcgcggaga acgtcgaggc gcgcgacggc cggatgcact tcacggtgct gcgcgagggg cgcgagccgc tgcccgtcgt cctgaacctg ccgggcctgc acaacgtgca gaacgcgctc gccgcgatcg cgatcgcgac cgatctcgac gtggccgaca cggcgattca gcaggcgctc gcggaattca acggcgtggg ccggcgcttc cagcgctacg gggagatgcc ggccgcgggc ggcggcgcgt acacgctgat cgacgactac ggccaccatc cggtcgagat ggcggcgacg atcgcggcgg cgcgcggcgc gttcccgggc cgccggctcg tgctcgcttt ccagccgcat cgctacacgc gcacgcgcga ctgcttcgac gatttcgtca acgtgctgtc gacggtcgac gcgctggtgc tgaccgaggt gtacgcggcc ggcgaggcgc cgatttcgac cgcgaacggc gatgcgctgt cgcgcgcgct gcgcgcggcg ggcaaggtcg agccggtatt cgtcgcgacg gtcgacgagg tgccggacgc gctcgcgaag ctcgcgcgcg ccggcgacgt ggtgatcacg atgggcgcgg gctcgatcgg cggggtgccg ggcaagctcg cgcaggacac gcaacagaag ggataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for ButhA.00137.b.A1.PW33329
PURIFICATION DATe: 8/12/2011
CONCENTRATION: 43.28mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKHIVKHIH FVGIGGAGMS GIAEVLVNLG YQVSGSDLAR NAVTERLEAL GARVSIGHDA ANIEGANAVV VSTAVRSDNP EVLAARRLRV PIVPRAVMLA ELMRLKQGIA IAGTHGKTTT TSLVASVLAA GGLDPTFVIG GRLTSAGANA RLGTGDFIVA EADESDASFL NLYPVIEVIT NIDADHMDTY GHDFARLKQA FIEFTQRLPF YGSAVVCIDD PNVRQIVPLI SKPVVRYGFA ADAQVRAENV EARDGRMHFT VLREGREPLP VVLNLPGLHN VQNALAAIAI ATDLDVADTA IQQALAEFNG VGRRFQRYGE MPAAGGGAYT LIDDYGHHPV EMAATIAAAR GAFPGRRLVL AFQPHRYTRT RDCFDDFVNV LSTVDALVLT EVYAAGEAPI STANGDALSR ALRAAGKVEP VFVATVDEVP DALAKLARAG DVVITMGAGS IGGVPGKLAQ DTQQ
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaac acatcgtcaa acacattcat ttcgtcggga tcggcggcgc gggcatgagc ggcatcgccg aagtgctcgt gaacctgggc tatcaggtga gcggctccga tctcgcgcgc aacgcggtga ccgagcgcct cgaggcgctc ggcgcgcgcg tgtcgatcgg ccacgacgcg gcgaacatcg agggcgcgaa cgcggtggtc gtgtcgaccg cggtgcgctc ggacaacccg gaggtgctcg ccgcgcgccg cctgcgcgtg ccgatcgtgc cgcgcgcggt gatgctcgcg gagctgatgc gcctgaagca ggggatcgcg atcgccggca cgcacggcaa gacgacgacg acgagcctcg tcgcgagcgt gctcgccgcg ggcgggctcg atccgacgtt cgtgatcggc gggcgtctca cgagcgccgg ggcgaacgcg cggctcggca cgggcgactt catcgtcgcg gaagccgacg agtcggacgc gtcgttcctg aacctgtatc cggtgatcga ggtcatcacg aacatcgacg ccgatcacat ggacacctac ggccacgatt tcgcgcggct caagcaggcg ttcatcgaat tcacgcagcg gctgccgttc tacggcagcg ccgtcgtgtg catcgacgac ccgaacgtgc ggcagatcgt gccgctgatc tcgaagcccg tcgtgcgcta cggcttcgcg gcggatgcgc aggtgcgcgc ggagaacgtc gaggcgcgcg acggccggat gcacttcacg gtgctgcgcg aggggcgcga gccgctgccc gtcgtcctga acctgccggg cctgcacaac gtgcagaacg cgctcgccgc gatcgcgatc gcgaccgatc tcgacgtggc cgacacggcg attcagcagg cgctcgcgga attcaacggc gtgggccggc gcttccagcg ctacggggag atgccggccg cgggcggcgg cgcgtacacg ctgatcgacg actacggcca ccatccggtc gagatggcgg cgacgatcgc ggcggcgcgc ggcgcgttcc cgggccgccg gctcgtgctc gctttccagc cgcatcgcta cacgcgcacg cgcgactgct tcgacgattt cgtcaacgtg ctgtcgacgg tcgacgcgct ggtgctgacc gaggtgtacg cggccggcga ggcgccgatt tcgaccgcga acggcgatgc gctgtcgcgc gcgctgcgcg cggcgggcaa ggtcgagccg gtattcgtcg cgacggtcga cgaggtgccg gacgcgctcg cgaagctcgc gcgcgccggc gacgtggtga tcacgatggg cgcgggctcg atcggcgggg tgccgggcaa gctcgcgcag gacacgcaac aga
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKHIVKHIH FVGIGGAGMS GIAEVLVNLG YQVSGSDLAR NAVTERLEAL GARVSIGHDA ANIEGANAVV VSTAVRSDNP EVLAARRLRV PIVPRAVMLA ELMRLKQGIA IAGTHGKTTT TSLVASVLAA GGLDPTFVIG GRLTSAGANA RLGTGDFIVA EADESDASFL NLYPVIEVIT NIDADHMDTY GHDFARLKQA FIEFTQRLPF YGSAVVCIDD PNVRQIVPLI SKPVVRYGFA ADAQVRAENV EARDGRMHFT VLREGREPLP VVLNLPGLHN VQNALAAIAI ATDLDVADTA IQQALAEFNG VGRRFQRYGE MPAAGGGAYT LIDDYGHHPV EMAATIAAAR GAFPGRRLVL AFQPHRYTRT RDCFDDFVNV LSTVDALVLT EVYAAGEAPI STANGDALSR ALRAAGKVEP VFVATVDEVP DALAKLARAG DVVITMGAGS IGGVPGKLAQ DTQQKG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAACACATC GTCAAACACA TTCATTTCGT CGGGATCGGC GGCGCGGGCA TGAGCGGCAT CGCCGAAGTG CTCGTGAACC TGGGCTATCA GGTGAGCGGC TCCGATCTCG CGCGCAACGC GGTGACCGAG CGCCTCGAGG CGCTCGGCGC GCGCGTGTCG ATCGGCCACG ACGCGGCGAA CATCGAGGGC GCGAACGCGG TGGTCGTGTC GACCGCGGTG CGCTCGGACA ACCCGGAGGT GCTCGCCGCG CGCCGCCTGC GCGTGCCGAT CGTGCCGCGC GCGGTGATGC TCGCGGAGCT GATGCGCCTG AAGCAGGGGA TCGCGATCGC CGGCACGCAC GGCAAGACGA CGACGACGAG CCTCGTCGCG AGCGTGCTCG CCGCGGGCGG GCTCGATCCG ACGTTCGTGA TCGGCGGGCG TCTCACGAGC GCCGGGGCGA ACGCGCGGCT CGGCACGGGC GACTTCATCG TCGCGGAAGC CGACGAGTCG GACGCGTCGT TCCTGAACCT GTATCCGGTG ATCGAGGTCA TCACGAACAT CGACGCCGAT CACATGGACA CCTACGGCCA CGATTTCGCG CGGCTCAAGC AGGCGTTCAT CGAATTCACG CAGCGGCTGC CGTTCTACGG CAGCGCCGTC GTGTGCATCG ACGACCCGAA CGTGCGGCAG ATCGTGCCGC TGATCTCGAA GCCCGTCGTG CGCTACGGCT TCGCGGCGGA TGCGCAGGTG CGCGCGGAGA ACGTCGAGGC GCGCGACGGC CGGATGCACT TCACGGTGCT GCGCGAGGGG CGCGAGCCGC TGCCCGTCGT CCTGAACCTG CCGGGCCTGC ACAACGTGCA GAACGCGCTC GCCGCGATCG CGATCGCGAC CGATCTCGAC GTGGCCGACA CGGCGATTCA GCAGGCGCTC GCGGAATTCA ACGGCGTGGG CCGGCGCTTC CAGCGCTACG GGGAGATGCC GGCCGCGGGC GGCGGCGCGT ACACGCTGAT CGACGACTAC GGCCACCATC CGGTCGAGAT GGCGGCGACG ATCGCGGCGG CGCGCGGCGC GTTCCCGGGC CGCCGGCTCG TGCTCGCTTT CCAGCCGCAT CGCTACACGC GCACGCGCGA CTGCTTCGAC GATTTCGTCA ACGTGCTGTC GACGGTCGAC GCGCTGGTGC TGACCGAGGT GTACGCGGCC GGCGAGGCGC CGATTTCGAC CGCGAACGGC GATGCGCTGT CGCGCGCGCT GCGCGCGGCG GGCAAGGTCG AGCCGGTATT CGTCGCGACG GTCGACGAGG TGCCGGACGC GCTCGCGAAG CTCGCGCGCG CCGGCGACGT GGTGATCACG ATGGGCGCGG GCTCGATCGG CGGGGTGCCG GGCAAGCTCG CGCAGGACAC GCAACAGAAG GGATAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA