SSGCID: Target Page

ButhA.00137.a

UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase

CENTER ID:	ButhA.00137.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00137.a.A2.GE32724	Mature protein( ButhA.00137.a )		22	470

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00137.a.A2.PS01452	Mature protein( ButhA.00137.a )		22	470

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.3924
RefSeq:	YP_441715.1
UniProt:	Q2SZD4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHIHILGICG TFMGGLAVLA RAAGHTVTGC DAGVYPPMST QLEAQGIRLI EGYGAEQIDL KPDLFVIGNV VSRGNPLMEA ILDRGLPYVS GPQWLGEHVL AGKWVLAVAG THGKTTTSSM LAWILEDAGL NPGFLIGGVP LNFGVSARLT DSSFFVIEAD EYDTAFFDKR SKFVHYRPRT AILNNLEFDH ADIFPDLAAI ETQFHHLVRT VPSVGRLVTN GREDALERVL SRGCWSDVER FGVDGGWQAL PAEDGVPADE RFAVYWRSER VGAVDWHVQG EHNRMNALAA IAAARHVGVP PAQAATALAT FRNVKRRMEV RGSVDGVTVY DDFAHHPTAI ETTIAGLRAR IGRENTRILA VLEPRSNTMK LGVMKAQLPA SLADADLVFG YGAPSGRDAL GWSLPDALAP LGDKARAFDD LHALVKAVTV SARPGDHVLV MSNGGFGGVH QKLLDALSAR GDAAPARGSM

NT Sequence

ATGCATATCC ACATTCTCGG CATCTGCGGC ACCTTCATGG GTGGTCTGGC CGTGCTCGCC CGCGCGGCGG GCCATACGGT GACCGGTTGC GACGCAGGCG TCTATCCGCC GATGAGCACG CAGCTCGAGG CGCAGGGCAT CCGGCTGATC GAGGGCTACG GCGCGGAACA GATCGACCTG AAGCCCGATC TGTTCGTGAT CGGCAACGTG GTGTCGCGCG GCAACCCGCT GATGGAGGCG ATCCTCGATC GAGGGTTGCC TTACGTGTCC GGACCGCAAT GGCTTGGCGA GCATGTGCTT GCAGGTAAGT GGGTGCTCGC TGTCGCCGGT ACGCACGGCA AGACGACGAC GAGTTCGATG CTCGCGTGGA TTCTCGAAGA CGCCGGCCTG AATCCGGGCT TCCTGATCGG CGGTGTGCCG CTGAACTTCG GTGTGTCCGC GCGGCTCACC GATTCGAGCT TCTTCGTGAT CGAGGCGGAC GAGTACGACA CGGCGTTCTT CGATAAGCGT TCGAAATTCG TCCATTACCG GCCTCGAACC GCGATCCTGA ATAACCTCGA ATTCGATCAT GCCGATATCT TTCCAGATCT TGCCGCGATC GAGACTCAAT TTCATCATTT GGTGCGCACG GTGCCGAGCG TCGGCCGGCT CGTGACGAAC GGCCGTGAAG ACGCGCTCGA GCGCGTGCTG TCGCGCGGTT GCTGGAGCGA TGTCGAGCGC TTCGGCGTCG ACGGCGGGTG GCAGGCGTTG CCCGCGGAGG ACGGCGTGCC GGCCGACGAG CGCTTCGCCG TGTACTGGCG CAGCGAGCGC GTCGGCGCCG TCGACTGGCA CGTGCAAGGC GAACACAACC GGATGAATGC ACTTGCGGCG ATCGCGGCCG CGCGTCACGT GGGCGTGCCG CCCGCGCAGG CGGCCACGGC GCTCGCGACG TTTCGCAACG TCAAGCGCCG GATGGAAGTG CGCGGCAGCG TCGACGGCGT GACTGTCTAC GACGATTTCG CACATCACCC GACCGCGATC GAGACGACGA TCGCCGGGCT TCGCGCGCGC ATCGGTCGTG AAAATACTCG CATCCTCGCC GTGCTGGAGC CGCGATCGAA CACGATGAAG CTCGGCGTGA TGAAGGCGCA GCTGCCGGCG AGCCTCGCCG ATGCGGATCT CGTATTCGGC TACGGCGCGC CGAGCGGCCG CGACGCGCTC GGCTGGAGCC TGCCGGACGC GCTCGCGCCG CTCGGCGACA AGGCGCGCGC TTTCGACGAT CTGCACGCGC TCGTGAAGGC CGTGACGGTG TCGGCGCGGC CGGGCGACCA CGTGCTCGTG ATGAGCAACG GCGGCTTCGG CGGCGTCCAC CAGAAGTTGC TCGACGCGCT GTCTGCGCGT GGCGACGCGG CGCCGGCCCG GGGCAGCATG

Details for ButhA.00137.a.A2.GE32724

HARVESTED ON:	6/20/2011
SEQUENCED ON:	7/14/2011
EXPECTED MW:	50kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	95
PERCENT COVERAGE:	51

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAAGHTVTG CDAXVYPPMS TQLEAQGIRL IEGYGAEQID LKPDLFVIGN VVSRGNPLME AILDRGLPYV SGPQWLGEHV LAGKWVLAVA GTHGKTTTSS MLAWILEDAG LNPGFLXGGV PLNFGVSARL TDSSFXXIEA DEYDTAFXXK RSKFXXYRPR TAILNNLEFD HADIFPDLAA IETXFHHLVR TVPSVXRLVT XGREDALERV LSRGCWSDVE RFX

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcgg cgggccatac ggtgaccggt tgcgacgcan gcgtctatcc gccgatgagc acgcagctcg aggcgcaggg catccggctg atcgagggct acggcgcgga acagatcgac ctgaagcccg atctgttcgt gatcggcaac gtggtgtcgc gcggcaaccc gctgatggag gcgatcctcg atcgagggtt gccttacgtg tccggaccgc aatggcttgg cgagcatgtg cttgcaggta agtgggtgct cgctgtcgcc ggtacgcacg gcaagacgac gacgagttcg atgctcgcgt ggattctcga agacgccggc ctgaatccgg gcttcctgat nggcggtgtg ccgctgaact tcggtgtgtc cgcgcggctc accgattcga gcttcttnnn gatcgaggcg gacgagtacg acacggcgtt cttnnntaag cgttcgaaat tcnnnnntta ccggcctcga accgcgatcc tgaataacct cgaattcgat catgccgata tctttccnga tcttgccgcg atcgagactc antttcatca tttggtgcgc acggtgccna gcgtcnnncg gctcgtgacg aanggncgtg aagacgcgct cgagcgcgtg ctgtcgcgcg gttgctggag cgatgtcgag cgcttcngcg tc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAAGHTVTG CDAGVYPPMS TQLEAQGIRL IEGYGAEQID LKPDLFVIGN VVSRGNPLME AILDRGLPYV SGPQWLGEHV LAGKWVLAVA GTHGKTTTSS MLAWILEDAG LNPGFLIGGV PLNFGVSARL TDSSFFVIEA DEYDTAFFDK RSKFVHYRPR TAILNNLEFD HADIFPDLAA IETQFHHLVR TVPSVGRLVT NGREDALERV LSRGCWSDVE RFGVDGGWQA LPAEDGVPAD ERFAVYWRSE RVGAVDWHVQ GEHNRMNALA AIAAARHVGV PPAQAATALA TFRNVKRRME VRGSVDGVTV YDDFAHHPTA IETTIAGLRA RIGRENTRIL AVLEPRSNTM KLGVMKAQLP ASLADADLVF GYGAPSGRDA LGWSLPDALA PLGDKARAFD DLHALVKAVT VSARPGDHVL VMSNGGFGGV HQKLLDALSA RGDAAPARGS M

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggcgggc catacggtga ccggttgcga cgcaggcgtc tatccgccga tgagcacgca gctcgaggcg cagggcatcc ggctgatcga gggctacggc gcggaacaga tcgacctgaa gcccgatctg ttcgtgatcg gcaacgtggt gtcgcgcggc aacccgctga tggaggcgat cctcgatcga gggttgcctt acgtgtccgg accgcaatgg cttggcgagc atgtgcttgc aggtaagtgg gtgctcgctg tcgccggtac gcacggcaag acgacgacga gttcgatgct cgcgtggatt ctcgaagacg ccggcctgaa tccgggcttc ctgatcggcg gtgtgccgct gaacttcggt gtgtccgcgc ggctcaccga ttcgagcttc ttcgtgatcg aggcggacga gtacgacacg gcgttcttcg ataagcgttc gaaattcgtc cattaccggc ctcgaaccgc gatcctgaat aacctcgaat tcgatcatgc cgatatcttt ccagatcttg ccgcgatcga gactcaattt catcatttgg tgcgcacggt gccgagcgtc ggccggctcg tgacgaacgg ccgtgaagac gcgctcgagc gcgtgctgtc gcgcggttgc tggagcgatg tcgagcgctt cggcgtcgac ggcgggtggc aggcgttgcc cgcggaggac ggcgtgccgg ccgacgagcg cttcgccgtg tactggcgca gcgagcgcgt cggcgccgtc gactggcacg tgcaaggcga acacaaccgg atgaatgcac ttgcggcgat cgcggccgcg cgtcacgtgg gcgtgccgcc cgcgcaggcg gccacggcgc tcgcgacgtt tcgcaacgtc aagcgccgga tggaagtgcg cggcagcgtc gacggcgtga ctgtctacga cgatttcgca catcacccga ccgcgatcga gacgacgatc gccgggcttc gcgcgcgcat cggtcgtgaa aatactcgca tcctcgccgt gctggagccg cgatcgaaca cgatgaagct cggcgtgatg aaggcgcagc tgccggcgag cctcgccgat gcggatctcg tattcggcta cggcgcgccg agcggccgcg acgcgctcgg ctggagcctg ccggacgcgc tcgcgccgct cggcgacaag gcgcgcgctt tcgacgatct gcacgcgctc gtgaaggccg tgacggtgtc ggcgcggccg ggcgaccacg tgctcgtgat gagcaacggc ggcttcggcg gcgtccacca gaagttgctc gacgcgctgt ctgcgcgtgg cgacgcggcg ccggcccggg gcagcatgta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a

Details for ButhA.00137.a.A2.PS01452

PURIFICATION DATe:	4/5/2012
CONCENTRATION:	31.2mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	110µl
PERCENT IDENTITY:	95
PERCENT COVERAGE:	51

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGGCGGGC CATACGGTGA CCGGTTGCGA CGCAGGCGTC TATCCGCCGA TGAGCACGCA GCTCGAGGCG CAGGGCATCC GGCTGATCGA GGGCTACGGC GCGGAACAGA TCGACCTGAA GCCCGATCTG TTCGTGATCG GCAACGTGGT GTCGCGCGGC AACCCGCTGA TGGAGGCGAT CCTCGATCGA GGGTTGCCTT ACGTGTCCGG ACCGCAATGG CTTGGCGAGC ATGTGCTTGC AGGTAAGTGG GTGCTCGCTG TCGCCGGTAC GCACGGCAAG ACGACGACGA GTTCGATGCT CGCGTGGATT CTCGAAGACG CCGGCCTGAA TCCGGGCTTC CTGATCGGCG GTGTGCCGCT GAACTTCGGT GTGTCCGCGC GGCTCACCGA TTCGAGCTTC TTCGTGATCG AGGCGGACGA GTACGACACG GCGTTCTTCG ATAAGCGTTC GAAATTCGTC CATTACCGGC CTCGAACCGC GATCCTGAAT AACCTCGAAT TCGATCATGC CGATATCTTT CCAGATCTTG CCGCGATCGA GACTCAATTT CATCATTTGG TGCGCACGGT GCCGAGCGTC GGCCGGCTCG TGACGAACGG CCGTGAAGAC GCGCTCGAGC GCGTGCTGTC GCGCGGTTGC TGGAGCGATG TCGAGCGCTT CGGCGTCGAC GGCGGGTGGC AGGCGTTGCC CGCGGAGGAC GGCGTGCCGG CCGACGAGCG CTTCGCCGTG TACTGGCGCA GCGAGCGCGT CGGCGCCGTC GACTGGCACG TGCAAGGCGA ACACAACCGG ATGAATGCAC TTGCGGCGAT CGCGGCCGCG CGTCACGTGG GCGTGCCGCC CGCGCAGGCG GCCACGGCGC TCGCGACGTT TCGCAACGTC AAGCGCCGGA TGGAAGTGCG CGGCAGCGTC GACGGCGTGA CTGTCTACGA CGATTTCGCA CATCACCCGA CCGCGATCGA GACGACGATC GCCGGGCTTC GCGCGCGCAT CGGTCGTGAA AATACTCGCA TCCTCGCCGT GCTGGAGCCG CGATCGAACA CGATGAAGCT CGGCGTGATG AAGGCGCAGC TGCCGGCGAG CCTCGCCGAT GCGGATCTCG TATTCGGCTA CGGCGCGCCG AGCGGCCGCG ACGCGCTCGG CTGGAGCCTG CCGGACGCGC TCGCGCCGCT CGGCGACAAG GCGCGCGCTT TCGACGATCT GCACGCGCTC GTGAAGGCCG TGACGGTGTC GGCGCGGCCG GGCGACCACG TGCTCGTGAT GAGCAACGGC GGCTTCGGCG GCGTCCACCA GAAGTTGCTC GACGCGCTGT CTGCGCGTGG CGACGCGGCG CCGGCCCGGG GCAGCATGTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A