ButhA.00134.a

4-hydroxyphenylpyruvate dioxygenase

CENTER ID: ButhA.00134.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00134.a.B1.GE36114 Full length( ButhA.00134.a ) 1 381
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.1356
RefSeq: YP_439418.1
UniProt: Q2T5Y1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSSASPASS DPAFAGPPGD NPLGMAGLEF VEFASREPDA LARRFEQLGF KAIARHVSKA VTLYRQGPMN FLVNAQPDSF AARYADEYGT GVCAIGIRVD DAQRAFERAI ELGAWAFEGE RIGVGELTIP AIQGIGASHI HFVDRWRGRG GLRGGVGDIS IFDVDFRPID VATAQADLDY FGAGLRRVDH LTQTVGRGRM QEWLDFYRDL LHFREIHELN ANWHVSEEAR VMVSPCGDVR IPVYEEGTRR TELMHEYLPD HPGEGVQHIA LATDDILACA DALAANGVEF VEPPARYYDE IEARLPGCRI DVDALRARRI LVDGEIGDDG VPRLFLQTFV KRRPGEIFFE IVERRGHHGF GEGNLRALAH ARNAARGALR Q
NT Sequence
ATGTCCAGCT CAGCCAGTCC CGCGTCCAGC GATCCCGCGT TTGCCGGCCC GCCGGGCGAC AACCCGCTCG GCATGGCGGG GCTCGAATTC GTCGAATTCG CGTCGCGCGA GCCTGACGCG CTCGCGCGGC GCTTCGAGCA GCTCGGTTTC AAGGCGATCG CGCGGCACGT CAGCAAGGCG GTCACGCTCT ACCGGCAAGG GCCGATGAAC TTTCTCGTGA ACGCGCAGCC CGATTCGTTC GCCGCGCGCT ACGCGGACGA ATATGGCACG GGCGTGTGTG CGATCGGCAT TCGCGTCGAC GACGCGCAGC GCGCGTTCGA GCGCGCGATC GAGCTCGGCG CGTGGGCGTT CGAGGGCGAG CGGATCGGCG TCGGCGAATT GACGATTCCG GCGATCCAGG GGATCGGCGC GTCGCACATC CATTTCGTCG ACCGCTGGCG CGGGCGCGGC GGATTGCGCG GCGGGGTCGG CGACATCTCG ATCTTCGACG TCGATTTCCG CCCGATCGAC GTCGCCACGG CGCAGGCGGA CCTCGACTAC TTCGGCGCCG GCCTGCGGCG CGTCGATCAC CTGACGCAGA CGGTCGGCCG TGGCCGGATG CAGGAGTGGC TCGATTTCTA TCGCGATCTG CTGCACTTCC GCGAGATCCA TGAACTCAAC GCGAACTGGC ACGTGTCGGA GGAGGCGCGC GTGATGGTGT CGCCGTGCGG CGACGTGCGG ATTCCGGTGT ACGAGGAGGG CACGAGGCGC ACCGAGCTGA TGCACGAGTA TCTGCCCGAC CATCCGGGCG AGGGGGTGCA GCACATCGCG CTCGCGACCG ACGACATTCT CGCGTGCGCG GACGCGCTCG CGGCGAACGG CGTCGAGTTC GTCGAGCCGC CCGCGCGCTA CTACGACGAG ATCGAGGCGC GATTGCCCGG CTGCCGGATC GACGTCGATG CGCTGCGCGC GCGCCGCATT CTCGTCGACG GCGAGATCGG CGACGACGGC GTGCCGAGGC TGTTTCTCCA GACGTTCGTC AAGCGCCGGC CCGGCGAGAT CTTCTTCGAG ATCGTCGAGC GGCGCGGGCA TCACGGCTTC GGCGAGGGCA ATCTGCGCGC GCTCGCGCAC GCGAGGAATG CGGCGCGCGG CGCGCTCAGG CAG
Details for ButhA.00134.a.B1.GE36114
HARVESTED ON: 9/26/2012
SEQUENCED ON: 9/25/2012
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 19
PERCENT COVERAGE: 90
Validated AA Sequence
MPAPLDDLEE DLAGPALDER LEKQPRHAVV ADLAVDENAA RAQRIDVDPA AGQSRLDLVV VARGRLDELD AVRRERVRAR ENVVGRERDV LHPLARMVGQ ILVHQLGAPR ALLVHRNPHV AARRHHHARL LRHVPVRVEF MDLAEVQQIA IEIEPLLHPA TADRLRQVID APQAGAEVVE VRLRRGDVDR AEIDVEDRDV ADPAAQSAAP APAVDEMDVR RADPLDRRNR QFADADPLAL ERPRAELDRA LERALRVVDA NADRTHARAI FVRVARGERI GLRVHEKVHR PLPVERDRLA DVPRDRLETE LLEAPRERVR LARREFDEFE PRHAERVVAR RAGKRGIAGR G
Validated NT Sequence
cgcagattgc cctcgccgaa gccgtgatgc ccgcgccgct cgacgatctc gaagaagatc tcgccgggcc ggcgcttgac gaacgtctgg agaaacagcc tcggcacgcc gtcgtcgccg atctcgccgt cgacgagaat gcggcgcgcg cgcagcgcat cgacgtcgat ccggcagccg ggcaatcgcg cctcgatctc gtcgtagtag cgcgcgggcg gctcgacgaa ctcgacgccg ttcgccgcga gcgcgtccgc gcacgcgaga atgtcgtcgg tcgcgagcgc gatgtgctgc accccctcgc ccggatggtc gggcagatac tcgtgcatca gctcggtgcg cctcgtgccc tcctcgtaca ccggaatccg cacgtcgccg cacggcgaca ccatcacgcg cgcctcctcc gacacgtgcc agttcgcgtt gagttcatgg atctcgcgga agtgcagcag atcgcgatag aaatcgagcc actcctgcat ccggccacgg ccgaccgtct gcgtcaggtg atcgacgcgc cgcaggcngg cgccgaagta gtcgaggtcc gcctgcgccg tggcgacgtc gatcgggcgg aaatcgacgt cgaagatcga gatgtcgccg accccgccgc gcaatccgcc gcgcccgcgc cagcggtcga cgaaatggat gtgcgacgcg ccgatcccct ggatcgccgg aatcgtcaat tcgccgacgc cgatccgctc gccctcgaac gcccacgcgc cgagctcgat cgcgcgctcg aacgcgcgct gcgcgtcgtc gacgcgaatg ccgatcgcac acacgcccgt gccatattcg tccgcgtagc gcgcggcgaa cgaatcgggc tgcgcgttca cgagaaagtt catcggccct tgccggtaga gcgtgaccgc cttgctgacg tgccgcgcga tcgccttgaa accgagctgc tcgaagcgcc gcgcgagcgc gtcaggctcg cgcgacgcga attcgacgaa ttcgagcccc gccatgccga gcgggttgtc gcccggcggg ccggcaaacg cgggatcgct ggacgcggga ct
Expected Protein Sequence
MAHHHHHHMS SSASPASSDP AFAGPPGDNP LGMAGLEFVE FASREPDALA RRFEQLGFKA IARHVSKAVT LYRQGPMNFL VNAQPDSFAA RYADEYGTGV CAIGIRVDDA QRAFERAIEL GAWAFEGERI GVGELTIPAI QGIGASHIHF VDRWRGRGGL RGGVGDISIF DVDFRPIDVA TAQADLDYFG AGLRRVDHLT QTVGRGRMQE WLDFYRDLLH FREIHELNAN WHVSEEARVM VSPCGDVRIP VYEEGTRRTE LMHEYLPDHP GEGVQHIALA TDDILACADA LAANGVEFVE PPARYYDEIE ARLPGCRIDV DALRARRILV DGEIGDDGVP RLFLQTFVKR RPGEIFFEIV ERRGHHGFGE GNLRALAHAR NAARGALRQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcca gctcagccag tcccgcgtcc agcgatcccg cgtttgccgg cccgccgggc gacaacccgc tcggcatggc ggggctcgaa ttcgtcgaat tcgcgtcgcg cgagcctgac gcgctcgcgc ggcgcttcga gcagctcggt ttcaaggcga tcgcgcggca cgtcagcaag gcggtcacgc tctaccggca agggccgatg aactttctcg tgaacgcgca gcccgattcg ttcgccgcgc gctacgcgga cgaatatggc acgggcgtgt gtgcgatcgg cattcgcgtc gacgacgcgc agcgcgcgtt cgagcgcgcg atcgagctcg gcgcgtgggc gttcgagggc gagcggatcg gcgtcggcga attgacgatt ccggcgatcc aggggatcgg cgcgtcgcac atccatttcg tcgaccgctg gcgcgggcgc ggcggattgc gcggcggggt cggcgacatc tcgatcttcg acgtcgattt ccgcccgatc gacgtcgcca cggcgcaggc ggacctcgac tacttcggcg ccggcctgcg gcgcgtcgat cacctgacgc agacggtcgg ccgtggccgg atgcaggagt ggctcgattt ctatcgcgat ctgctgcact tccgcgagat ccatgaactc aacgcgaact ggcacgtgtc ggaggaggcg cgcgtgatgg tgtcgccgtg cggcgacgtg cggattccgg tgtacgagga gggcacgagg cgcaccgagc tgatgcacga gtatctgccc gaccatccgg gcgagggggt gcagcacatc gcgctcgcga ccgacgacat tctcgcgtgc gcggacgcgc tcgcggcgaa cggcgtcgag ttcgtcgagc cgcccgcgcg ctactacgac gagatcgagg cgcgattgcc cggctgccgg atcgacgtcg atgcgctgcg cgcgcgccgc attctcgtcg acggcgagat cggcgacgac ggcgtgccga ggctgtttct ccagacgttc gtcaagcgcc ggcccggcga gatcttcttc gagatcgtcg agcggcgcgg gcatcacggc ttcggcgagg gcaatctgcg cgcgctcgcg cacgcgagga atgcggcgcg cggcgcgctc aggcagtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t