SSGCID: Target Page

ButhA.00133.a

Cysteinyl-tRNA synthetase (EC 6.1.1.16) (Cysteine--tRNA ligase) (CysRS) (BTH_I1941) (cysS)

CENTER ID:	ButhA.00133.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00133.a.A1.GE32723	full length		1	465

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00133.a.A1.PW35855	full length		1	465

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.4748
RefSeq:	YP_442471.1
UniProt:	Q2SX78

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MESLRIYNTL ARDKQDFVPR QPGEVRMYVC GITVYDYCHI GHARMVVVFD IVQRWLRAAG YRVTYVRNIT DIDDKIIRRA LENGETIQSL TRRFIDAMNA DFDALGVERP DIEPRATDFI PQMLGMIEKL EANGYAYQAK DGDVNYSVRK FANYGKLSGK SLEDLRAGER VAAKDAKDDP LDFVLWKRAK PEEPAGASWE SKYGAGRPGW HIECSAMGCT LLGEHFDIHG GGQDLQFPHH ENEIAQSEGA TGQTFVNYWM HNGFVQVDSE KMSKSLGNFF TIREVLEKYD AEVVRFFIVR THYRSPLNYS DVHLDDARAS LTRLYTALKD VTPDAAPLDW SEAHAQRFAA AMNDDFNTAV AVAVLFELAT EVNRTREPAL ARQLKRLAGL LGLLGREPRE FLQQAAGAAR TGALEPDEIE ARIAARVAAK QAKNYAEADR IRAELLEAGI ALEDKPGGST EWRRV

NT Sequence

atggaatcac tgcgcatcta caacacgctc gcgcgtgaca agcaagactt cgtgccgcgg cagcccggcg aagtgcggat gtacgtctgc gggatcaccg tctacgacta ttgccatatc ggccatgcgc ggatggtggt cgtgttcgac atcgtccagc gctggctgcg cgcggccggc tatcgcgtga cgtacgtgcg caacatcacc gacatcgacg acaagatcat tcgccgcgcg ctcgagaacg gcgagacgat ccagtcgctc acgcgccgct tcatcgatgc gatgaacgcg gatttcgacg cgctcggcgt cgagcggccg gacatcgagc cgcgcgcgac cgatttcatc ccgcagatgc tcgggatgat cgagaagctc gaggcgaacg ggtacgccta tcaggcgaag gatggcgacg tcaactattc ggtgcgcaag ttcgcgaact acggcaagct gtccggcaag tcgctcgagg acttgcgcgc gggcgaacgc gtcgccgcga aggacgcgaa ggacgacccg ctcgatttcg tgctgtggaa acgcgcgaag cctgaggagc cggccggcgc gtcgtgggaa tcgaagtacg gcgcgggccg cccgggctgg cacatcgaat gctctgcgat gggctgcacg ctgctcggcg agcacttcga cattcacggc ggcggccagg acctgcagtt cccgcatcac gagaacgaga tcgcgcagag cgagggtgcg accggacaaa ctttcgtcaa ttattggatg cacaacggct tcgtgcaggt cgatagtgag aagatgtcga agtcgctcgg caacttcttc acgatccgcg aagtgctgga gaagtacgat gccgaggtcg tgcgcttctt catcgtgcgc acgcactatc gttcgccgct caactacagc gacgttcatc tcgacgacgc gcgcgcgtcg ctcacgcgtc tttacacggc gctgaaggac gtgacgccgg atgcggcgcc gctcgactgg agcgaggcgc acgcgcagcg tttcgcggcc gcgatgaacg acgacttcaa cacggcggtc gcggtcgccg tgctgttcga gctcgcgacc gaggtgaacc gcacgcgcga gccggcgctc gcgcgccagc tcaagcggct cgcgggcctg ctcggcctgc tcggccgcga gccgcgcgag ttcctgcagc aggcggcggg cgccgcgcgc acgggcgcgc tcgagccgga cgagatcgaa gcgaggatcg ccgcgcgcgt cgcggcgaag caggcgaaga actatgccga agcggaccgg atccgcgcgg aactgctcga agccgggatc gcacttgaag acaaaccggg cggatcgacc gagtggcgtc gcgtatga

Details for ButhA.00133.a.A1.GE32723

HARVESTED ON:	6/20/2011
SEQUENCED ON:	7/14/2011
EXPECTED MW:	54kDa
OBSERVED MW:	55kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MGTLEAQTQG PGSMESLRIY NTLARDKQDF VPRQPGEVRM YVCGITVYDY CHIGHARMVV VFDIVQRWLR AAGYRVTYVR NITDIDDKII RRALENGETI QSLTRRFIDA MNADFDALGV ERPDIEPRAT DFIPQMLGMI EKLEANGYAY QAKDGDVNYS VRKFANYGKL SGKSLEDLRA GERVAAKDAK DDPLDFVLWK RAKPEEPAGA SWESKYGAGR PGWHIECSAM GCTLLGEHFD IHGGGQDLQF PHHENEIAQS EGATGQTFVN YWMHNGFVQV DSEKMSKSLG NFFTIREVLE KYDAEVVRFF IVRTHYRSPL NYSDVHLDDA RASLTRLYTA LKDVTPDAAP LDWSEAHAQR FAAAMNDDFN TAVAVAVLFE LATEVNRTRE PALARQLKRL AGLLGLLGRE PREFLQQAAG AARTGALEPD EIEARIAARV AAKQAKNYAE ADRIRAELLE AGIALEDKPG GSTECVAYN

Validated NT Sequence

ttattatacg cgacgcactc ggtcgatccg cccggtttgt cttcaagtgc gatcccggct tcgagcagtt ccgcgcggat ccggtccgct tcggcatagt tcttcgcctg cttcgccgcg acgcgcgcgg cgatcctcgc ttcgatctcg tccggctcga gcgcgcccgt gcgcgcggcg cccgccgcct gctgcaggaa ctcgcgcggc tcgcggccga gcaggccgag caggcccgcg agccgcttga gctggcgcgc gagcgccggc tcgcgcgtgc ggttcacctc ggtcgcgagc tcgaacagca cggcgaccgc gaccgccgtg ttgaagtcgt cgttcatcgc ggccgcgaaa cgctgcgcgt gcgcctcgct ccagtcgagc ggcgccgcat ccggcgtcac gtccttcagc gccgtgtaaa gacgcgtgag cgacgcgcgc gcgtcgtcga gatgaacgtc gctgtagttg agcggcgaac gatagtgcgt gcgcacgatg aagaagcgca cgacctcggc atcgtacttc tccagcactt cgcggatcgt gaagaagttg ccgagcgact tcgacatctt ctcactatcg acctgcacga agccgttgtg catccaataa ttgacgaaag tttgtccggt cgcaccctcg ctctgcgcga tctcgttctc gtgatgcggg aactgcaggt cctggccgcc gccgtgaatg tcgaagtgct cgccgagcag cgtgcagccc atcgcagagc attcgatgtg ccagcccggg cggcccgcgc cgtacttcga ttcccacgac gcgccggccg gctcctcagg cttcgcgcgt ttccacagca cgaaatcgag cgggtcgtcc ttcgcgtcct tcgcggcgac gcgttcgccc gcgcgcaagt cctcgagcga cttgccggac agcttgccgt agttcgcgaa cttgcgcacc gaatagttga cgtcgccatc cttcgcctga taggcgtacc cgttcgcctc gagcttctcg atcatcccga gcatctgcgg gatgaaatcg gtcgcgcgcg gctcgatgtc cggccgctcg acgccgagcg cgtcgaaatc cgcgttcatc gcatcgatga agcggcgcgt gagcgactgg atcgtctcgc cgttctcgag cgcgcggcga atgatcttgt cgtcgatgtc ggtgatgttg cgcacgtacg tcacgcgata gccggccgcg cgcagccagc gctggacgat gtcgaacacg accaccatcc gcgcatggcc gatatggcaa tagtcgtaga cggtgatccc gcagacgtac atccgcactt cgccgggctg ccgcggcacg aagtcttgct tgtcacgcgc gagcgtgttg tagatgcgca gtgattccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc ca

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMESLRIYNT LARDKQDFVP RQPGEVRMYV CGITVYDYCH IGHARMVVVF DIVQRWLRAA GYRVTYVRNI TDIDDKIIRR ALENGETIQS LTRRFIDAMN ADFDALGVER PDIEPRATDF IPQMLGMIEK LEANGYAYQA KDGDVNYSVR KFANYGKLSG KSLEDLRAGE RVAAKDAKDD PLDFVLWKRA KPEEPAGASW ESKYGAGRPG WHIECSAMGC TLLGEHFDIH GGGQDLQFPH HENEIAQSEG ATGQTFVNYW MHNGFVQVDS EKMSKSLGNF FTIREVLEKY DAEVVRFFIV RTHYRSPLNY SDVHLDDARA SLTRLYTALK DVTPDAAPLD WSEAHAQRFA AAMNDDFNTA VAVAVLFELA TEVNRTREPA LARQLKRLAG LLGLLGREPR EFLQQAAGAA RTGALEPDEI EARIAARVAA KQAKNYAEAD RIRAELLEAG IALEDKPGGS TEWRRV

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaatcactg cgcatctaca acacgctcgc gcgtgacaag caagacttcg tgccgcggca gcccggcgaa gtgcggatgt acgtctgcgg gatcaccgtc tacgactatt gccatatcgg ccatgcgcgg atggtggtcg tgttcgacat cgtccagcgc tggctgcgcg cggccggcta tcgcgtgacg tacgtgcgca acatcaccga catcgacgac aagatcattc gccgcgcgct cgagaacggc gagacgatcc agtcgctcac gcgccgcttc atcgatgcga tgaacgcgga tttcgacgcg ctcggcgtcg agcggccgga catcgagccg cgcgcgaccg atttcatccc gcagatgctc gggatgatcg agaagctcga ggcgaacggg tacgcctatc aggcgaagga tggcgacgtc aactattcgg tgcgcaagtt cgcgaactac ggcaagctgt ccggcaagtc gctcgaggac ttgcgcgcgg gcgaacgcgt cgccgcgaag gacgcgaagg acgacccgct cgatttcgtg ctgtggaaac gcgcgaagcc tgaggagccg gccggcgcgt cgtgggaatc gaagtacggc gcgggccgcc cgggctggca catcgaatgc tctgcgatgg gctgcacgct gctcggcgag cacttcgaca ttcacggcgg cggccaggac ctgcagttcc cgcatcacga gaacgagatc gcgcagagcg agggtgcgac cggacaaact ttcgtcaatt attggatgca caacggcttc gtgcaggtcg atagtgagaa gatgtcgaag tcgctcggca acttcttcac gatccgcgaa gtgctggaga agtacgatgc cgaggtcgtg cgcttcttca tcgtgcgcac gcactatcgt tcgccgctca actacagcga cgttcatctc gacgacgcgc gcgcgtcgct cacgcgtctt tacacggcgc tgaaggacgt gacgccggat gcggcgccgc tcgactggag cgaggcgcac gcgcagcgtt tcgcggccgc gatgaacgac gacttcaaca cggcggtcgc ggtcgccgtg ctgttcgagc tcgcgaccga ggtgaaccgc acgcgcgagc cggcgctcgc gcgccagctc aagcggctcg cgggcctgct cggcctgctc ggccgcgagc cgcgcgagtt cctgcagcag gcggcgggcg ccgcgcgcac gggcgcgctc gagccggacg agatcgaagc gaggatcgcc gcgcgcgtcg cggcgaagca ggcgaagaac tatgccgaag cggaccggat ccgcgcggaa ctgctcgaag ccgggatcgc acttgaagac aaaccgggcg gatcgaccga gtggcgtcgc gtataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for ButhA.00133.a.A1.PW35855

PURIFICATION DATe:	3/16/2012
CONCENTRATION:	24mg/ml
OBSERVED MW:	48kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	110µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAATCACTG CGCATCTACA ACACGCTCGC GCGTGACAAG CAAGACTTCG TGCCGCGGCA GCCCGGCGAA GTGCGGATGT ACGTCTGCGG GATCACCGTC TACGACTATT GCCATATCGG CCATGCGCGG ATGGTGGTCG TGTTCGACAT CGTCCAGCGC TGGCTGCGCG CGGCCGGCTA TCGCGTGACG TACGTGCGCA ACATCACCGA CATCGACGAC AAGATCATTC GCCGCGCGCT CGAGAACGGC GAGACGATCC AGTCGCTCAC GCGCCGCTTC ATCGATGCGA TGAACGCGGA TTTCGACGCG CTCGGCGTCG AGCGGCCGGA CATCGAGCCG CGCGCGACCG ATTTCATCCC GCAGATGCTC GGGATGATCG AGAAGCTCGA GGCGAACGGG TACGCCTATC AGGCGAAGGA TGGCGACGTC AACTATTCGG TGCGCAAGTT CGCGAACTAC GGCAAGCTGT CCGGCAAGTC GCTCGAGGAC TTGCGCGCGG GCGAACGCGT CGCCGCGAAG GACGCGAAGG ACGACCCGCT CGATTTCGTG CTGTGGAAAC GCGCGAAGCC TGAGGAGCCG GCCGGCGCGT CGTGGGAATC GAAGTACGGC GCGGGCCGCC CGGGCTGGCA CATCGAATGC TCTGCGATGG GCTGCACGCT GCTCGGCGAG CACTTCGACA TTCACGGCGG CGGCCAGGAC CTGCAGTTCC CGCATCACGA GAACGAGATC GCGCAGAGCG AGGGTGCGAC CGGACAAACT TTCGTCAATT ATTGGATGCA CAACGGCTTC GTGCAGGTCG ATAGTGAGAA GATGTCGAAG TCGCTCGGCA ACTTCTTCAC GATCCGCGAA GTGCTGGAGA AGTACGATGC CGAGGTCGTG CGCTTCTTCA TCGTGCGCAC GCACTATCGT TCGCCGCTCA ACTACAGCGA CGTTCATCTC GACGACGCGC GCGCGTCGCT CACGCGTCTT TACACGGCGC TGAAGGACGT GACGCCGGAT GCGGCGCCGC TCGACTGGAG CGAGGCGCAC GCGCAGCGTT TCGCGGCCGC GATGAACGAC GACTTCAACA CGGCGGTCGC GGTCGCCGTG CTGTTCGAGC TCGCGACCGA GGTGAACCGC ACGCGCGAGC CGGCGCTCGC GCGCCAGCTC AAGCGGCTCG CGGGCCTGCT CGGCCTGCTC GGCCGCGAGC CGCGCGAGTT CCTGCAGCAG GCGGCGGGCG CCGCGCGCAC GGGCGCGCTC GAGCCGGACG AGATCGAAGC GAGGATCGCC GCGCGCGTCG CGGCGAAGCA GGCGAAGAAC TATGCCGAAG CGGACCGGAT CCGCGCGGAA CTGCTCGAAG CCGGGATCGC ACTTGAAGAC AAACCGGGCG GATCGACCGA GTGGCGTCGC GTATAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA