ButhA.00125.b

Chemotaxis protein methyltransferase CheR

CENTER ID: ButhA.00125.b
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00125.b.B1.GE36113 Full length( ButhA.00125.b ) 1 273
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.174
RefSeq: YP_438361.1
UniProt: Q2T8Y8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNSVDFEYTD ADFRRIARHV YQRAGIVLTE AKRSLAYGRL VRRLRVLGIG RFSDYLDRLE ANRSSDEWQH FVNALTTNLT SFFRESYHFD ILAAQMQAAS GPFRIWCAAA STGEEPYSLA MTACDAHGTA ANRVEIVASD IDTKVLAAAQ AGIYKLHQLD KMPLRRKQAY FQRGSGSNGG AVRVKPALRG MVTFRQINLL TEPWPLDGPF DAVFCRNVLI YFDAPTQQKV LRNMARHLKP DGRLYVGHSE NLRALDDVYA PCGRAVYELV NGR
NT Sequence
ATGAATAGCG TGGATTTCGA ATACACGGAT GCGGATTTCC GCCGGATTGC GCGGCATGTT TATCAGCGTG CGGGGATCGT GCTGACCGAG GCGAAGCGCT CGCTTGCGTA CGGACGCCTG GTGCGCCGCT TACGTGTGCT GGGCATCGGG CGCTTTTCGG ACTATCTCGA TCGACTCGAA GCCAATCGGT CGAGTGACGA ATGGCAGCAT TTCGTCAACG CGCTGACGAC CAATTTGACG TCATTCTTTC GCGAGAGCTA CCACTTCGAC ATTCTCGCCG CGCAGATGCA GGCGGCTTCG GGGCCGTTCC GGATCTGGTG CGCGGCGGCG AGCACGGGCG AGGAGCCTTA CTCGCTCGCG ATGACCGCGT GCGATGCGCA CGGGACGGCG GCGAACCGCG TCGAGATCGT CGCCTCCGAC ATCGACACGA AGGTGCTCGC CGCGGCGCAG GCCGGCATCT ACAAGCTGCA TCAGCTCGAC AAGATGCCGC TGCGCCGCAA GCAGGCCTAT TTTCAGCGAG GCAGCGGCAG CAACGGCGGC GCGGTGCGCG TCAAGCCCGC GCTGCGCGGC ATGGTCACGT TCCGGCAGAT CAATCTGCTG ACGGAACCGT GGCCGCTCGA TGGCCCGTTC GACGCGGTCT TCTGCCGAAA CGTCCTGATT TATTTCGACG CGCCGACGCA GCAGAAGGTG TTGCGCAACA TGGCTCGGCA CCTGAAGCCG GACGGTCGCC TATACGTCGG TCACTCCGAA AACCTGAGGG CCCTCGACGA TGTCTACGCA CCGTGCGGCC GCGCGGTCTA CGAGCTCGTG AATGGGCGT
Details for ButhA.00125.b.B1.GE36113
HARVESTED ON: 9/26/2012
SEQUENCED ON: 9/25/2012
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 93
PERCENT COVERAGE: 101
Validated AA Sequence
MAHHHHHHMN SVDFEYTDAD FRRXARHVYQ RAGIVLTEAK RSLAYGRLVR RLXVLGIGRF SDYLDRLEAN RSSDEWQHFV NALTTNLTSF FRESYHFDIL AAQMQAASGP FRIWCAAAST GEEPYSLAMT ACDAHGTAAN RVEIVASDID TKVLAAAQAG IYKLHQLDKM PLRRKQAYFQ RGSGSNGGAV RVKPALRGMV TFRQINLLTE PWPLDGPFDA VFCRNVLIYF DAPTQQKVLR NMARHLKPDG RLYVGHSENL RALDXXYXXX XPXXXXXXXX XXXXX
Validated NT Sequence
annnnnntnn nnnnntttaa naaggagann taccatggct caccaccacc accaccatat gaatagcgtg gatttcgaat acacggatgc ggatttccgc cggatngcgc ggcatgttta tcagcgtgcg gggatcgtgc tgaccgaggc gaagcgctcg cttgcgtacg gacgcctggt gcgccgctta cntgtgctgg gcatcgggcg cttttcggac tatctcgatc gactcgaagc caatcggtcg agtgacgaat ggcagcattt cgtcaacgcg ctgacgacca atttgacgtc attctttcgc gagagctacc acttcgacat tctcgccgcg cagatgcagg cggcttcggg gccgttccgg atctggtgcg cggcggcgag cacgggcgag gagccttact cgctcgcgat gaccgcgtgc gatgcgcacg ggacggcggc gaaccgcgtc gagatcgtcg cctccgacat cgacacgaag gtgctcgccg cggcgcaggc cggcatctac aagctgcatc agctcgacaa gatgccgctg cgccgcaagc aggcctattt tcagcgaggc agcggcagca acggcggcgc ggtgcgcgtc aagcccgcgc tgcgcggcat ggtcacgttc cggcagatca atctgctgac ggaaccgtgg ccgctcgatg gcccgttcga cgcggtcttc tgccgaaacg tcctgattta tttcgacgcg ccgacgcagc agaaggtgtt gcgcaacatg gctcggcacc tgaagccgga cggtcgccta tacgtcggtc actccgaaaa cctgagggcc ctcgacgann tctacnnnnn cnngnngccc nnnnnnnnnn nnannnnnnn nnnangggnn nntantgant aanatnnnnn nnnnnnnnnn nnnnnnnnnn nnnanannna aaaaaancnc nnaanntann ngncnnntnn tggtcnnngn ngnnnnnnnn nnnncnnnnn nnganannnn nnnnnnnnnn ngannnnnnn nnnnnnnnnn nncnnnnnnn ncgnnnnnnn nnnntnnnnn nnnnnnnnnn nnnnnncaaa nnaanannnn nnnnnnnnnn nnnnnngnnn ntnnnnnnnn nnncncgcnn aannnnnnnn nntnnnnnnn nnnnnnnnnn nnnnnnncnn nntnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnng
Expected Protein Sequence
MAHHHHHHMN SVDFEYTDAD FRRIARHVYQ RAGIVLTEAK RSLAYGRLVR RLRVLGIGRF SDYLDRLEAN RSSDEWQHFV NALTTNLTSF FRESYHFDIL AAQMQAASGP FRIWCAAAST GEEPYSLAMT ACDAHGTAAN RVEIVASDID TKVLAAAQAG IYKLHQLDKM PLRRKQAYFQ RGSGSNGGAV RVKPALRGMV TFRQINLLTE PWPLDGPFDA VFCRNVLIYF DAPTQQKVLR NMARHLKPDG RLYVGHSENL RALDDVYAPC GRAVYELVNG R
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata gcgtggattt cgaatacacg gatgcggatt tccgccggat tgcgcggcat gtttatcagc gtgcggggat cgtgctgacc gaggcgaagc gctcgcttgc gtacggacgc ctggtgcgcc gcttacgtgt gctgggcatc gggcgctttt cggactatct cgatcgactc gaagccaatc ggtcgagtga cgaatggcag catttcgtca acgcgctgac gaccaatttg acgtcattct ttcgcgagag ctaccacttc gacattctcg ccgcgcagat gcaggcggct tcggggccgt tccggatctg gtgcgcggcg gcgagcacgg gcgaggagcc ttactcgctc gcgatgaccg cgtgcgatgc gcacgggacg gcggcgaacc gcgtcgagat cgtcgcctcc gacatcgaca cgaaggtgct cgccgcggcg caggccggca tctacaagct gcatcagctc gacaagatgc cgctgcgccg caagcaggcc tattttcagc gaggcagcgg cagcaacggc ggcgcggtgc gcgtcaagcc cgcgctgcgc ggcatggtca cgttccggca gatcaatctg ctgacggaac cgtggccgct cgatggcccg ttcgacgcgg tcttctgccg aaacgtcctg atttatttcg acgcgccgac gcagcagaag gtgttgcgca acatggctcg gcacctgaag ccggacggtc gcctatacgt cggtcactcc gaaaacctga gggccctcga cgatgtctac gcaccgtgcg gccgcgcggt ctacgagctc gtgaatgggc gttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat