SSGCID: Target Page

ButhA.00120.a

GTPase obg

CENTER ID:	ButhA.00120.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00120.a.A1.GE32419	Full length( ButhA.00120.a )		1	372

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.3897
RefSeq:	YP_441689.1
UniProt:	Q2SZG0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKFIDEARIE VIAGDGGDGS ASMRREKFVP FGGPDGGDGG RGGSVYVIAD RNINTLIDYR YAKKHMARNG ENGRGSDCYG KGGDDVTLRM PVGTVINDMD TGELIADLTE HDQKVLVAKG GAGGLGNLHF KSSTNRAPRQ KTDGKPGERR MLKLELKVLA DVGLLGMPNA GKSTFISSVS NAKPKIADYP FTTLAPNLGV VRVGPGKSFV IADIPGLIEG AAEGAGLGHQ FLRHLQRTGL LLHLVDLAPF DESVDPVAEA KAIVGELRKY DESLYQKPRW LVLNKLDMVP EDERRTRVVD FIERFGWTGP VFEISALTGQ GCEGLVYAIY DYLAEHSDAH RAELAEDLAS DVRFRDASGA GGEPHEREAD AP

NT Sequence

ATGAAGTTCA TTGACGAAGC GCGAATCGAG GTCATCGCCG GGGACGGGGG CGATGGCAGC GCGTCGATGC GCCGCGAGAA ATTCGTTCCG TTCGGCGGAC CGGACGGCGG CGACGGCGGC CGCGGCGGCA GCGTGTACGT GATCGCGGAT CGCAACATCA ATACGCTGAT CGACTACCGG TACGCGAAGA AGCACATGGC GCGCAACGGC GAGAACGGCC GCGGCTCGGA TTGCTACGGC AAGGGCGGCG ACGACGTCAC GCTGCGCATG CCGGTCGGCA CCGTCATCAA CGACATGGAT ACGGGCGAGC TGATCGCCGA TCTGACCGAG CACGACCAGA AGGTGCTCGT CGCGAAGGGC GGCGCGGGCG GCCTCGGCAA CCTGCATTTC AAGTCGAGCA CGAACCGCGC GCCGCGCCAG AAGACGGACG GCAAGCCGGG CGAGCGGCGC ATGCTGAAGC TCGAACTGAA GGTGCTCGCG GACGTCGGCC TGCTCGGGAT GCCGAACGCG GGCAAATCGA CGTTCATTTC GTCGGTGTCG AACGCGAAGC CGAAGATCGC CGATTATCCG TTCACGACGC TTGCGCCGAA TCTCGGCGTG GTGCGCGTCG GGCCGGGCAA GAGCTTCGTG ATCGCCGATA TTCCGGGTCT GATCGAAGGC GCGGCGGAAG GCGCGGGCCT CGGCCATCAG TTTCTGCGGC ATTTGCAGCG CACGGGTTTG CTGCTGCATC TCGTCGATCT CGCGCCGTTC GATGAAAGCG TCGATCCCGT CGCGGAGGCG AAGGCGATCG TCGGCGAGCT GCGCAAGTAC GACGAATCGC TGTATCAGAA GCCACGCTGG CTCGTGTTGA ACAAGCTCGA CATGGTGCCG GAGGACGAGC GCCGCACGCG CGTCGTCGAT TTCATCGAGC GCTTCGGCTG GACGGGCCCC GTGTTCGAGA TTTCCGCGCT GACGGGGCAG GGCTGCGAAG GCCTTGTCTA TGCGATCTAC GATTATCTCG CCGAGCATTC GGATGCGCAT CGCGCCGAGC TCGCCGAGGA TCTGGCTTCG GACGTGCGCT TTCGCGACGC GTCGGGCGCA GGCGGCGAGC CGCACGAGCG CGAAGCCGAC GCGCCC

Details for ButhA.00120.a.A1.GE32419

HARVESTED ON:	11/8/2011
SEQUENCED ON:	11/11/2011
EXPECTED MW:	42kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	89
PERCENT COVERAGE:	60

Validated AA Sequence

Validated NT Sequence

ttttgtttna ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaagt tcattgacga agcgcgaatc gaggtcatcg ccggggacgg gggcgatggc agcgcgtcga tgcgccgcga gaaattcgtt ccgttcggcg gaccggacgg cggcgacggc ggccgcggcg gcagcgtgta cgtgatcgcg gatcgcaaca tcaatacgct gatcgactac cggtacgcga agaagcacat ggcgcgcaac ggcgagaacg gccgcggctc ggattgctac ggcaagggcg gcgacgacgt cacgctgcgc atgccggtcg gcaccgtcat caacgacatg gatacgggcg agctgatcgc cgatctgacc gagcacgacc agaaggtgct cgtcgcgaag ggcggcgcgg gcggcctcgg caacctgcat ttcaagtcga gcacgaaccg cgcgccgcgc cagaagacgg acggcaagcc gggcgagcgg cgcatgctga agctcgaact gaaggtgctc gcggacgtcg gcctgctcgg gatgccgaac gcgggcaaat cgacgttcat ttcgtcggtg tcgaacgcga agccgacgcg ccctaataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gcnnnnnc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMKFIDEARI EVIAGDGGDG SASMRREKFV PFGGPDGGDG GRGGSVYVIA DRNINTLIDY RYAKKHMARN GENGRGSDCY GKGGDDVTLR MPVGTVINDM DTGELIADLT EHDQKVLVAK GGAGGLGNLH FKSSTNRAPR QKTDGKPGER RMLKLELKVL ADVGLLGMPN AGKSTFISSV SNAKPKIADY PFTTLAPNLG VVRVGPGKSF VIADIPGLIE GAAEGAGLGH QFLRHLQRTG LLLHLVDLAP FDESVDPVAE AKAIVGELRK YDESLYQKPR WLVLNKLDMV PEDERRTRVV DFIERFGWTG PVFEISALTG QGCEGLVYAI YDYLAEHSDA HRAELAEDLA SDVRFRDASG AGGEPHEREA DAP

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagttcatt gacgaagcgc gaatcgaggt catcgccggg gacgggggcg atggcagcgc gtcgatgcgc cgcgagaaat tcgttccgtt cggcggaccg gacggcggcg acggcggccg cggcggcagc gtgtacgtga tcgcggatcg caacatcaat acgctgatcg actaccggta cgcgaagaag cacatggcgc gcaacggcga gaacggccgc ggctcggatt gctacggcaa gggcggcgac gacgtcacgc tgcgcatgcc ggtcggcacc gtcatcaacg acatggatac gggcgagctg atcgccgatc tgaccgagca cgaccagaag gtgctcgtcg cgaagggcgg cgcgggcggc ctcggcaacc tgcatttcaa gtcgagcacg aaccgcgcgc cgcgccagaa gacggacggc aagccgggcg agcggcgcat gctgaagctc gaactgaagg tgctcgcgga cgtcggcctg ctcgggatgc cgaacgcggg caaatcgacg ttcatttcgt cggtgtcgaa cgcgaagccg aagatcgccg attatccgtt cacgacgctt gcgccgaatc tcggcgtggt gcgcgtcggg ccgggcaaga gcttcgtgat cgccgatatt ccgggtctga tcgaaggcgc ggcggaaggc gcgggcctcg gccatcagtt tctgcggcat ttgcagcgca cgggtttgct gctgcatctc gtcgatctcg cgccgttcga tgaaagcgtc gatcccgtcg cggaggcgaa ggcgatcgtc ggcgagctgc gcaagtacga cgaatcgctg tatcagaagc cacgctggct cgtgttgaac aagctcgaca tggtgccgga ggacgagcgc cgcacgcgcg tcgtcgattt catcgagcgc ttcggctgga cgggccccgt gttcgagatt tccgcgctga cggggcaggg ctgcgaaggc cttgtctatg cgatctacga ttatctcgcc gagcattcgg atgcgcatcg cgccgagctc gccgaggatc tggcttcgga cgtgcgcttt cgcgacgcgt cgggcgcagg cggcgagccg cacgagcgcg aagccgacgc gccctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa