SSGCID: Target Page

ButhA.00119.a

D-alanine--D-alanine ligase (EC 6.3.2.4) (D-Ala-D-Ala ligase) (D-alanylalanine synthetase) (BTH_I1120) (ddl)

CENTER ID:	ButhA.00119.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00119.a.A1.GE32722	full length		1	312

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00119.a.A1.PW33326	full length		1	312

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.3876
RefSeq:	YP_441668.1
UniProt:	Q2SZI1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSGIDPKRFG KVAVLLGGES AERDVSLNSG RLVLQGLRDA GIDAHPFDPA QRPLAALKDE GFVRAFNALH GGYGENGQIQ GALDFYGIRY TGSGVLGSAL GLDKFRTKLV WQQTGIPTPP FETVMRGDDY AARAKDIVAK LGMPLFVKPA SEGSSVAVEK VKSADALPAA LEEAAKHDKI VIVEKSIEGG GEYTACIAAD LDLPLIKIVP AGEFYDYHAK YIANDTQYLI PCGLDAAKEA EFKRIARRAF DVLGCTDWGR ADFMLDAAGN PYFLEVNTAP GMTDHSLPPK AARAVGISYS ELVVKVLSLT LD

NT Sequence

atgagcggga tcgatccgaa acgtttcggc aaggtggcgg tgctgctcgg cggggaatcc gccgagcgcg atgtgtcgct gaactccggc cggctggtgc tgcagggctt gcgcgacgca ggcatcgacg cgcatccgtt cgatccggcg cagcggccgc tcgctgcgct caaggacgaa ggcttcgtgc gcgcgttcaa cgcgttgcac ggcggttacg gcgagaacgg tcagatccag ggcgcgctcg atttctacgg catccgctac acgggcagcg gcgtgctcgg ctcggcgctc gggctcgaca agttccgcac gaagctcgtc tggcagcaga cgggcattcc gacgccgccg ttcgagacgg tgatgcgcgg ggacgactac gcggcgcgcg cgaaggacat cgtcgcgaag ctcggcatgc cgctcttcgt gaagccggcg agcgagggct cgagcgtcgc ggtcgagaag gtgaaaagcg ccgatgcgtt gcccgccgca ctggaagaag cggcgaagca cgacaagatc gtgatcgtcg agaagagcat cgaaggcggc ggcgaataca ccgcgtgcat cgccgccgat ctcgatttgc cgctcatcaa gatcgtgcct gcgggcgagt tctacgacta tcacgcgaag tacatcgcga acgacacgca atatctgatt ccgtgcggcc tggatgcggc aaaggaagcc gagttcaagc gcatcgcgcg ccgcgcgttc gacgtgctcg gctgcacgga ttgggggcgc gcggatttca tgctcgatgc cgcaggcaac ccgtatttcc tcgaagtgaa caccgcgccg gggatgaccg atcactcgct gccgccgaag gcggcgcgcg cggtcggcat cagctattcg gagctggtgg tgaaagtgct gtcgctcacg ctcgattga

Details for ButhA.00119.a.A1.GE32722

HARVESTED ON:	6/20/2011
SEQUENCED ON:	7/14/2011
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMSGIDPK RFGKVAVLLG GESAERDVSL NSGRLVLQGL RDAGIDAHPF DPAQRPLAAL KDEGFVRAFN ALHGGYGENG QIQGALDFYG IRYTGSGVLG SALGLDKFRT KLVWQQTGIP TPPFETVMRG DDYAARAKDI VAKLGMPLFV KPASEGSSVA VEKVKSADAL PAALEEAAKH DKIVIVEKSI EGGGEYTACI AADLDLPLIK IVPAGEFYDY HAKYIANDTQ YLIPCGLDAA KEAEFKRIAR RAFDVLGCTD WGRADFMLDA AGNPYFLEVN TAPGMTDHSL PPKAARAVGI SYSELVVKVL SLTLD

Validated NT Sequence

tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgagcgg gatcgatccg aaacgtttcg gcaaggtggc ggtgctgctc ggcggggaat ccgccgagcg cgatgtgtcg ctgaactccg gccggctggt gctgcagggc ttgcgcgacg caggcatcga cgcgcatccg ttcgatccgg cgcagcggcc gctcgctgcg ctcaaggacg aaggcttcgt gcgcgcgttc aacgcgttgc acggcggtta cggcgagaac ggtcagatcc agggcgcgct cgatttctac ggcatccgct acacgggcag cggcgtgctc ggctcggcgc tcgggctcga caagttccgc acgaagctcg tctggcagca gacgggcatt ccgacgccgc cgttcgagac ggtgatgcgc ggggacgact acgcggcgcg cgcgaaggac atcgtcgcga agctcggcat gccgctcttc gtgaagccgg cgagcgaggg ctcgagcgtc gcggtcgaga aggtgaaaag cgccgatgcg ttgcccgccg cactggaaga agcggcgaag cacgacaaga tcgtgatcgt cgagaagagc atcgaaggcg gcggcgaata caccgcgtgc atcgccgccg atctcgattt gccgctcatc aagatcgtgc ctgcgggcga gttctacgac tatcacgcga agtacatcgc gaacgacacg caatatctga ttccgtgcgg cctggatgcg gcaaaggaag ccgagttcaa gcgcatcgcg cgccgcgcgt tcgacgtgct cggctgcacg gattgggggc gcgcggattt catgctcgat gccgcaggca acccgtattt cctcgaagtg aacaccgcgc cggggatgac cgatcactcg ctgccgccga aggcggcgcg cgcggtcggc atcagctatt cggagctggt ggtgaaagtg ctgtcgctca cgctcgatt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSGIDPKRF GKVAVLLGGE SAERDVSLNS GRLVLQGLRD AGIDAHPFDP AQRPLAALKD EGFVRAFNAL HGGYGENGQI QGALDFYGIR YTGSGVLGSA LGLDKFRTKL VWQQTGIPTP PFETVMRGDD YAARAKDIVA KLGMPLFVKP ASEGSSVAVE KVKSADALPA ALEEAAKHDK IVIVEKSIEG GGEYTACIAA DLDLPLIKIV PAGEFYDYHA KYIANDTQYL IPCGLDAAKE AEFKRIARRA FDVLGCTDWG RADFMLDAAG NPYFLEVNTA PGMTDHSLPP KAARAVGISY SELVVKVLSL TLD

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgggatc gatccgaaac gtttcggcaa ggtggcggtg ctgctcggcg gggaatccgc cgagcgcgat gtgtcgctga actccggccg gctggtgctg cagggcttgc gcgacgcagg catcgacgcg catccgttcg atccggcgca gcggccgctc gctgcgctca aggacgaagg cttcgtgcgc gcgttcaacg cgttgcacgg cggttacggc gagaacggtc agatccaggg cgcgctcgat ttctacggca tccgctacac gggcagcggc gtgctcggct cggcgctcgg gctcgacaag ttccgcacga agctcgtctg gcagcagacg ggcattccga cgccgccgtt cgagacggtg atgcgcgggg acgactacgc ggcgcgcgcg aaggacatcg tcgcgaagct cggcatgccg ctcttcgtga agccggcgag cgagggctcg agcgtcgcgg tcgagaaggt gaaaagcgcc gatgcgttgc ccgccgcact ggaagaagcg gcgaagcacg acaagatcgt gatcgtcgag aagagcatcg aaggcggcgg cgaatacacc gcgtgcatcg ccgccgatct cgatttgccg ctcatcaaga tcgtgcctgc gggcgagttc tacgactatc acgcgaagta catcgcgaac gacacgcaat atctgattcc gtgcggcctg gatgcggcaa aggaagccga gttcaagcgc atcgcgcgcc gcgcgttcga cgtgctcggc tgcacggatt gggggcgcgc ggatttcatg ctcgatgccg caggcaaccc gtatttcctc gaagtgaaca ccgcgccggg gatgaccgat cactcgctgc cgccgaaggc ggcgcgcgcg gtcggcatca gctattcgga gctggtggtg aaagtgctgt cgctcacgct cgattaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for ButhA.00119.a.A1.PW33326

PURIFICATION DATe:	8/12/2011
CONCENTRATION:	22.47mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	16
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGGGATC GATCCGAAAC GTTTCGGCAA GGTGGCGGTG CTGCTCGGCG GGGAATCCGC CGAGCGCGAT GTGTCGCTGA ACTCCGGCCG GCTGGTGCTG CAGGGCTTGC GCGACGCAGG CATCGACGCG CATCCGTTCG ATCCGGCGCA GCGGCCGCTC GCTGCGCTCA AGGACGAAGG CTTCGTGCGC GCGTTCAACG CGTTGCACGG CGGTTACGGC GAGAACGGTC AGATCCAGGG CGCGCTCGAT TTCTACGGCA TCCGCTACAC GGGCAGCGGC GTGCTCGGCT CGGCGCTCGG GCTCGACAAG TTCCGCACGA AGCTCGTCTG GCAGCAGACG GGCATTCCGA CGCCGCCGTT CGAGACGGTG ATGCGCGGGG ACGACTACGC GGCGCGCGCG AAGGACATCG TCGCGAAGCT CGGCATGCCG CTCTTCGTGA AGCCGGCGAG CGAGGGCTCG AGCGTCGCGG TCGAGAAGGT GAAAAGCGCC GATGCGTTGC CCGCCGCACT GGAAGAAGCG GCGAAGCACG ACAAGATCGT GATCGTCGAG AAGAGCATCG AAGGCGGCGG CGAATACACC GCGTGCATCG CCGCCGATCT CGATTTGCCG CTCATCAAGA TCGTGCCTGC GGGCGAGTTC TACGACTATC ACGCGAAGTA CATCGCGAAC GACACGCAAT ATCTGATTCC GTGCGGCCTG GATGCGGCAA AGGAAGCCGA GTTCAAGCGC ATCGCGCGCC GCGCGTTCGA CGTGCTCGGC TGCACGGATT GGGGGCGCGC GGATTTCATG CTCGATGCCG CAGGCAACCC GTATTTCCTC GAAGTGAACA CCGCGCCGGG GATGACCGAT CACTCGCTGC CGCCGAAGGC GGCGCGCGCG GTCGGCATCA GCTATTCGGA GCTGGTGGTG AAAGTGCTGT CGCTCACGCT CGATTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA