ButhA.00118.d

UTP-glucose-1-phosphate uridylyltransferase

CENTER ID: ButhA.00118.d
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00118.d.B1.GE36111 Full length( ButhA.00118.d ) 1 294
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.786
RefSeq: YP_438898.1
UniProt: Q2T7F1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKVTKAVFPV AGLGTRFLPA TKASPKEMLP VVDKPLIQYA VEEAINAGIT EMIFVTGRSK RAIEDHFDKS FEIESELEAR GKEKLLELVR GIKPSHVDCF YVRQPAALGL GHAVLCAEKL VHGEPFAVIL ADDLLHGDQP VLKQLVDVFN HYHSSVIGVE TIERDDSRSY GVVEGREWEE DIIKLSGIIE KPAPEHAPSN LGVVGRYVLM PSIFEHLRKI KPGAGGELQL TDAVQSLLTE EQVLAYRYYG TRFDCGSKLG YLKATIELAL QHPEVGREFE AYLRNCLPAL AAVA
NT Sequence
TTGAAAGTCA CCAAAGCCGT GTTCCCCGTT GCCGGTCTCG GCACACGGTT TTTGCCCGCC ACCAAGGCGA GTCCGAAGGA AATGCTGCCC GTCGTCGACA AGCCGCTGAT CCAGTATGCG GTCGAAGAGG CGATCAATGC GGGCATCACC GAGATGATCT TCGTGACGGG CCGCAGCAAG CGCGCGATCG AAGATCACTT CGACAAATCG TTCGAGATCG AATCCGAGCT CGAGGCGCGC GGCAAGGAAA AGCTGCTCGA GCTCGTGCGC GGCATCAAGC CGAGCCACGT CGACTGCTTC TACGTGCGCC AGCCGGCCGC GCTCGGCCTG GGCCACGCGG TGCTCTGCGC GGAGAAGCTC GTGCACGGCG AGCCGTTCGC GGTGATCCTC GCCGACGACT TGCTGCACGG CGACCAGCCG GTGCTCAAGC AGCTCGTCGA CGTCTTCAAT CACTATCACA GCTCGGTGAT CGGCGTCGAG ACGATCGAGC GCGATGACAG CCGCTCGTAC GGCGTCGTCG AGGGCCGCGA GTGGGAAGAG GACATCATCA AGCTGTCGGG CATCATCGAG AAGCCGGCGC CCGAGCATGC GCCTTCGAAT CTCGGCGTCG TCGGCCGCTA CGTGCTGATG CCGAGCATCT TCGAGCATCT GCGCAAGATC AAGCCGGGCG CGGGCGGCGA GCTGCAACTG ACGGACGCGG TGCAGTCGCT GCTGACCGAG GAGCAGGTGC TCGCATACCG ATACTACGGC ACCCGCTTCG ATTGCGGCAG CAAGCTCGGC TATCTGAAGG CGACGATCGA GCTCGCGCTG CAGCATCCGG AAGTCGGCCG CGAATTCGAG GCATACCTGC GCAACTGCCT GCCGGCGCTC GCCGCGGTCG CT
Details for ButhA.00118.d.B1.GE36111
HARVESTED ON: 9/26/2012
SEQUENCED ON: 9/25/2012
EXPECTED MW: 33kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MLKVTKAVFP VAGLGTRFLP ATKASPKEML PVVDKPLIQY AVEEAINAGI TEMIFVTGRS KRAIEDHFDK SFEIESELEA RGKEKLLELV RGIKPSHVDC FYVRQPAALG LGHAVLCAEK LVHGEPFAVI LADDLLHGDQ PVLKQLVDVF NHYHSSVIGV ETIERDDSRS YGVVEGREWE EDIIKLSGII EKPAPEHAPS NLGVVGRYVL MPSIFEHLRK IKPGAGGELQ LTDAVQSLLT EEQVLAYRYY GTRFDCGSKL GYLKATIELA LQHPEVGREF EAYLRNCLPA LAAVA
Validated NT Sequence
cttaagcgac cgcggcgagc gccggcaggc agttgcgcag gtatgcctcg aattcgcggc cgacttccgg atgctgcagc gcgagctcga tcgtcgcctt cagatagccg agcttgctgc cgcaatcgaa gcgggtgccg tagtatcggt atgcgagcac ctgctcctcg gtcagcagcg actgcaccgc gtccgtcagt tgcagctcgc cgcccgcgcc cggcttgatc ttgcgcagat gctcgaagat gctcggcatc agcacgtagc ggccgacgac gccgagattc gaaggcgcat gctcgggcgc cggcttctcg atgatgcccg acagcttgat gatgtcctct tcccactcgc ggccctcgac gacgccgtac gagcggctgt catcgcgctc gatcgtctcg acgccgatca ccgagctgtg atagtgattg aagacgtcga cgagctgctt gagcaccggc tggtcgccgt gcagcaagtc gtcggcgagg atcaccgcga acggctcgcc gtgcacgagc ttctccgcgc agagcaccgc gtggcccagg ccgagcgcgg ccggctggcg cacgtagaag cagtcgacgt ggctcggctt gatgccgcgc acgagctcga gcagcttttc cttgccgcgc gcctcgagct cggattcgat ctcgaacgat ttgtcgaagt gatcttcgat cgcgcgcttg ctgcggcccg tcacgaagat catctcggtg atgcccgcat tgatcgcctc ttcgaccgca tactggatca gcggcttgtc gacgacgggc agcatttcct tcggactcgc cttggtggcg ggcaaaaacc gtgtgccgag accggcaacg gggaacacgg ctttggtgac tttcaacata tagtggt
Expected Protein Sequence
MAHHHHHHML KVTKAVFPVA GLGTRFLPAT KASPKEMLPV VDKPLIQYAV EEAINAGITE MIFVTGRSKR AIEDHFDKSF EIESELEARG KEKLLELVRG IKPSHVDCFY VRQPAALGLG HAVLCAEKLV HGEPFAVILA DDLLHGDQPV LKQLVDVFNH YHSSVIGVET IERDDSRSYG VVEGREWEED IIKLSGIIEK PAPEHAPSNL GVVGRYVLMP SIFEHLRKIK PGAGGELQLT DAVQSLLTEE QVLAYRYYGT RFDCGSKLGY LKATIELALQ HPEVGREFEA YLRNCLPALA AVA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttga aagtcaccaa agccgtgttc cccgttgccg gtctcggcac acggtttttg cccgccacca aggcgagtcc gaaggaaatg ctgcccgtcg tcgacaagcc gctgatccag tatgcggtcg aagaggcgat caatgcgggc atcaccgaga tgatcttcgt gacgggccgc agcaagcgcg cgatcgaaga tcacttcgac aaatcgttcg agatcgaatc cgagctcgag gcgcgcggca aggaaaagct gctcgagctc gtgcgcggca tcaagccgag ccacgtcgac tgcttctacg tgcgccagcc ggccgcgctc ggcctgggcc acgcggtgct ctgcgcggag aagctcgtgc acggcgagcc gttcgcggtg atcctcgccg acgacttgct gcacggcgac cagccggtgc tcaagcagct cgtcgacgtc ttcaatcact atcacagctc ggtgatcggc gtcgagacga tcgagcgcga tgacagccgc tcgtacggcg tcgtcgaggg ccgcgagtgg gaagaggaca tcatcaagct gtcgggcatc atcgagaagc cggcgcccga gcatgcgcct tcgaatctcg gcgtcgtcgg ccgctacgtg ctgatgccga gcatcttcga gcatctgcgc aagatcaagc cgggcgcggg cggcgagctg caactgacgg acgcggtgca gtcgctgctg accgaggagc aggtgctcgc ataccgatac tacggcaccc gcttcgattg cggcagcaag ctcggctatc tgaaggcgac gatcgagctc gcgctgcagc atccggaagt cggccgcgaa ttcgaggcat acctgcgcaa ctgcctgccg gcgctcgccg cggtcgcttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat