ButhA.00118.b

UTP-glucose-1-phosphate uridylyltransferase

CENTER ID: ButhA.00118.b
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00118.b.B1.GE36109 Full length( ButhA.00118.b ) 1 293
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00118.b.B1.PS01678 Full length( ButhA.00118.b ) 1 293

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.4135
RefSeq: YP_441910.1
UniProt: Q2SYT9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKVTKAVFPV AGLGTRFLPA TKASPKEMLP VVDKPLIQYA VEEAIAAGIT EMIFVTGRSK RAIEDHFDKS YEIEAELEAR GKEKLLSLVR SIKPRHVDCF YVRQAEALGL GHAVLCAQKL VGDSPFAVIL ADDLLDGRPP VLRQMIDVFN HYHTSVIGVE EIAPQDSKSY GVIDGKRWED DLFKLSGIVE KPDPAAAPSN FGVVGRYVLT PRIFDHLRAL KPGAGGELQL TDALQSLLKD EQVLAHRYDG TRFDCGSKIG YLKATVEFAL RHPEVATEFE RYLSERFPEL VAA
NT Sequence
TTGAAAGTCA CCAAAGCCGT ATTCCCCGTC GCCGGCCTCG GCACCCGCTT TCTGCCCGCA ACGAAAGCGA GTCCGAAGGA AATGTTGCCG GTCGTCGACA AACCTCTGAT TCAATATGCG GTCGAGGAAG CGATCGCCGC AGGCATCACC GAGATGATCT TCGTCACGGG CCGTAGCAAG CGCGCGATCG AGGATCACTT CGACAAGTCA TACGAGATCG AGGCCGAACT CGAGGCGCGC GGCAAGGAAA AACTGCTGTC GCTCGTGCGC AGCATCAAGC CGCGGCACGT CGACTGTTTC TACGTGCGCC AGGCCGAGGC GCTCGGGCTC GGTCATGCGG TGCTGTGCGC GCAGAAGCTC GTCGGCGACA GCCCGTTCGC CGTCATCCTC GCGGACGATC TGCTCGACGG CCGGCCGCCC GTGCTGCGGC AGATGATCGA CGTGTTCAAT CACTATCACA CGTCGGTCAT CGGCGTCGAA GAGATCGCGC CGCAAGATTC GAAGTCGTAT GGCGTGATCG ACGGCAAGCG CTGGGAGGAC GATCTCTTCA AGCTGTCGGG CATCGTCGAG AAGCCCGATC CCGCCGCCGC GCCGTCCAAT TTCGGCGTCG TCGGCCGCTA TGTGCTGACG CCGCGCATCT TCGATCATCT GCGCGCGCTC AAGCCCGGCG CCGGCGGAGA ACTGCAATTG ACCGACGCGC TGCAATCGCT GCTGAAGGAC GAGCAAGTGC TCGCGCATCG TTACGACGGC ACACGCTTCG ATTGCGGCAG CAAGATCGGC TATCTGAAGG CGACGGTCGA GTTCGCGCTG CGTCACCCCG AAGTCGCGAC GGAATTCGAG CGGTACCTGA GCGAGCGTTT CCCGGAGCTC GTCGCGGCG
Details for ButhA.00118.b.B1.GE36109
HARVESTED ON: 9/26/2012
SEQUENCED ON: 9/27/2012
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MLKVTKAVFP VAGLGTRFLP ATKASPKEML PVVDKPLIQY AVEEAIAAGI TEMIFVTGRS KRAIEDHFDK SYEIEAELEA RGKEKLLSLV RSIKPRHVDC FYVRQAEALG LGHAVLCAQK LVGDSPFAVI LADDLLDGRP PVLRQMIDVF NHYHTSVIGV EEIAPQDSKS YGVIDGKRWE DDLFKLSGIV EKPDPAAAPS NFGVVGRYVL TPRIFDHLRA LKPGAGGELQ LTDALQSLLK DEQVLAHRYD GTRFDCGSKI GYLKATVEFA LRHPEVATEF ERYLSERFPE
Validated NT Sequence
agctccggga aacgctcgct caggtaccgc tcgaattccg tcgcgacttc ggggtgacgc agcgcgaact cgaccgtcgc cttcagatag ccgatcttgc tgccgcaatc gaagcgtgtg ccgtcgtaac gatgcgcgag cacttgctcg tccttcagca gcgattgcag cgcgtcggtc aattgcagtt ctccgccggc gccgggcttg agcgcgcgca gatgatcgaa gatgcgcggc gtcagcacat agcggccgac gacgccgaaa ttggacggcg cggcggcggg atcgggcttc tcgacgatgc ccgacagctt gaagagatcg tcctcccagc gcttgccgtc gatcacgcca tacgacttcg aatcttgcgg cgcgatctct tcgacgccga tgaccgacgt gtgatagtga ttgaacacgt cgatcatctg ccgcagcacg ggcggccggc cgtcgagcag atcgtccgcg aggatgacgg cgaacgggct gtcgccgacg agcttctgcg cgcacagcac cgcatgaccg agcccgagcg cctcggcctg gcgcacgtag aaacagtcga cgtgccgcgg cttgatgctg cgcacgagcg acagcagttt ttccttgccg cgcgcctcga gttcggcctc gatctcgtat gacttgtcga agtgatcctc gatcgcgcgc ttgctacggc ccgtgacgaa gatcatctcg gtgatgcctg cggcgatcgc ttcctcgacc gcatattgaa tcagaggttt gtcgacgacc ggcaacattt ccttcggact cgctttcgtt gcgggcagaa agcgggtgcc gaggccggcg acggggaata cggctttggt gactttcaac at
Expected Protein Sequence
MAHHHHHHML KVTKAVFPVA GLGTRFLPAT KASPKEMLPV VDKPLIQYAV EEAIAAGITE MIFVTGRSKR AIEDHFDKSY EIEAELEARG KEKLLSLVRS IKPRHVDCFY VRQAEALGLG HAVLCAQKLV GDSPFAVILA DDLLDGRPPV LRQMIDVFNH YHTSVIGVEE IAPQDSKSYG VIDGKRWEDD LFKLSGIVEK PDPAAAPSNF GVVGRYVLTP RIFDHLRALK PGAGGELQLT DALQSLLKDE QVLAHRYDGT RFDCGSKIGY LKATVEFALR HPEVATEFER YLSERFPELV AA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttga aagtcaccaa agccgtattc cccgtcgccg gcctcggcac ccgctttctg cccgcaacga aagcgagtcc gaaggaaatg ttgccggtcg tcgacaaacc tctgattcaa tatgcggtcg aggaagcgat cgccgcaggc atcaccgaga tgatcttcgt cacgggccgt agcaagcgcg cgatcgagga tcacttcgac aagtcatacg agatcgaggc cgaactcgag gcgcgcggca aggaaaaact gctgtcgctc gtgcgcagca tcaagccgcg gcacgtcgac tgtttctacg tgcgccaggc cgaggcgctc gggctcggtc atgcggtgct gtgcgcgcag aagctcgtcg gcgacagccc gttcgccgtc atcctcgcgg acgatctgct cgacggccgg ccgcccgtgc tgcggcagat gatcgacgtg ttcaatcact atcacacgtc ggtcatcggc gtcgaagaga tcgcgccgca agattcgaag tcgtatggcg tgatcgacgg caagcgctgg gaggacgatc tcttcaagct gtcgggcatc gtcgagaagc ccgatcccgc cgccgcgccg tccaatttcg gcgtcgtcgg ccgctatgtg ctgacgccgc gcatcttcga tcatctgcgc gcgctcaagc ccggcgccgg cggagaactg caattgaccg acgcgctgca atcgctgctg aaggacgagc aagtgctcgc gcatcgttac gacggcacac gcttcgattg cggcagcaag atcggctatc tgaaggcgac ggtcgagttc gcgctgcgtc accccgaagt cgcgacggaa ttcgagcggt acctgagcga gcgtttcccg gagctcgtcg cggcgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for ButhA.00118.b.B1.PS01678
PURIFICATION DATe: 11/20/2012
CONCENTRATION: 80mg/ml
OBSERVED MW: 34kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MLKVTKAVFP VAGLGTRFLP ATKASPKEML PVVDKPLIQY AVEEAIAAGI TEMIFVTGRS KRAIEDHFDK SYEIEAELEA RGKEKLLSLV RSIKPRHVDC FYVRQAEALG LGHAVLCAQK LVGDSPFAVI LADDLLDGRP PVLRQMIDVF NHYHTSVIGV EEIAPQDSKS YGVIDGKRWE DDLFKLSGIV EKPDPAAAPS NFGVVGRYVL TPRIFDHLRA LKPGAGGELQ LTDALQSLLK DEQVLAHRYD GTRFDCGSKI GYLKATVEFA LRHPEVATEF ERYLSERFPE
Validated NT Sequence
agctccggga aacgctcgct caggtaccgc tcgaattccg tcgcgacttc ggggtgacgc agcgcgaact cgaccgtcgc cttcagatag ccgatcttgc tgccgcaatc gaagcgtgtg ccgtcgtaac gatgcgcgag cacttgctcg tccttcagca gcgattgcag cgcgtcggtc aattgcagtt ctccgccggc gccgggcttg agcgcgcgca gatgatcgaa gatgcgcggc gtcagcacat agcggccgac gacgccgaaa ttggacggcg cggcggcggg atcgggcttc tcgacgatgc ccgacagctt gaagagatcg tcctcccagc gcttgccgtc gatcacgcca tacgacttcg aatcttgcgg cgcgatctct tcgacgccga tgaccgacgt gtgatagtga ttgaacacgt cgatcatctg ccgcagcacg ggcggccggc cgtcgagcag atcgtccgcg aggatgacgg cgaacgggct gtcgccgacg agcttctgcg cgcacagcac cgcatgaccg agcccgagcg cctcggcctg gcgcacgtag aaacagtcga cgtgccgcgg cttgatgctg cgcacgagcg acagcagttt ttccttgccg cgcgcctcga gttcggcctc gatctcgtat gacttgtcga agtgatcctc gatcgcgcgc ttgctacggc ccgtgacgaa gatcatctcg gtgatgcctg cggcgatcgc ttcctcgacc gcatattgaa tcagaggttt gtcgacgacc ggcaacattt ccttcggact cgctttcgtt gcgggcagaa agcgggtgcc gaggccggcg acggggaata cggctttggt gactttcaac at
Expressed Protein Sequence
MAHHHHHHML KVTKAVFPVA GLGTRFLPAT KASPKEMLPV VDKPLIQYAV EEAIAAGITE MIFVTGRSKR AIEDHFDKSY EIEAELEARG KEKLLSLVRS IKPRHVDCFY VRQAEALGLG HAVLCAQKLV GDSPFAVILA DDLLDGRPPV LRQMIDVFNH YHTSVIGVEE IAPQDSKSYG VIDGKRWEDD LFKLSGIVEK PDPAAAPSNF GVVGRYVLTP RIFDHLRALK PGAGGELQLT DALQSLLKDE QVLAHRYDGT RFDCGSKIGY LKATVEFALR HPEVATEFER YLSERFPELV AA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTGA AAGTCACCAA AGCCGTATTC CCCGTCGCCG GCCTCGGCAC CCGCTTTCTG CCCGCAACGA AAGCGAGTCC GAAGGAAATG TTGCCGGTCG TCGACAAACC TCTGATTCAA TATGCGGTCG AGGAAGCGAT CGCCGCAGGC ATCACCGAGA TGATCTTCGT CACGGGCCGT AGCAAGCGCG CGATCGAGGA TCACTTCGAC AAGTCATACG AGATCGAGGC CGAACTCGAG GCGCGCGGCA AGGAAAAACT GCTGTCGCTC GTGCGCAGCA TCAAGCCGCG GCACGTCGAC TGTTTCTACG TGCGCCAGGC CGAGGCGCTC GGGCTCGGTC ATGCGGTGCT GTGCGCGCAG AAGCTCGTCG GCGACAGCCC GTTCGCCGTC ATCCTCGCGG ACGATCTGCT CGACGGCCGG CCGCCCGTGC TGCGGCAGAT GATCGACGTG TTCAATCACT ATCACACGTC GGTCATCGGC GTCGAAGAGA TCGCGCCGCA AGATTCGAAG TCGTATGGCG TGATCGACGG CAAGCGCTGG GAGGACGATC TCTTCAAGCT GTCGGGCATC GTCGAGAAGC CCGATCCCGC CGCCGCGCCG TCCAATTTCG GCGTCGTCGG CCGCTATGTG CTGACGCCGC GCATCTTCGA TCATCTGCGC GCGCTCAAGC CCGGCGCCGG CGGAGAACTG CAATTGACCG ACGCGCTGCA ATCGCTGCTG AAGGACGAGC AAGTGCTCGC GCATCGTTAC GACGGCACAC GCTTCGATTG CGGCAGCAAG ATCGGCTATC TGAAGGCGAC GGTCGAGTTC GCGCTGCGTC ACCCCGAAGT CGCGACGGAA TTCGAGCGGT ACCTGAGCGA GCGTTTCCCG GAGCTCGTCG CGGCGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT