ButhA.00116.a

4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262) (4-(phosphohydroxy)-L-threonine dehydrogenase) (BTH_I0577) (pdxA)

CENTER ID: ButhA.00116.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00116.a.A1.GE32881 full length 1 349
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00116.a.A1.PS01184 full length 1 349

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.3266
RefSeq: YP_441134.1
UniProt: Q2T115

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSARPQPLRI AITTGEPAGV GPELTARALA DAATRWPDAH FTVLGDAGLI AGRAAAAGVD WGRVTAGGET GHVADAHVAD AHVANAHVAV AHRALAAPAE AGKLNPANGR YVLALLDAAI DGALAGKYDA IVTAPLQKST INDAGVPFTG HTEYLAERTH TPRVVMMLAG TGERPLRVAL ATTHLPLKDV AAALTIDGLV GTLSIIDRDL RERFGLAAPR ILVTGLNPHA GENGYLGREE IDVIEPALER ARAAGIDARG PYPADTLFQP RHLEHADCVL AMFHDQGLPV LKYATFGEGI NVTLGLPIIR TSVDHGTALD LAGTGRADPG SLVAAIDTAV TMARHQRAG
NT Sequence
atgtccgccc gtccgcagcc gctgcgcatc gcgatcacga ccggcgagcc ggccggcgtc ggccccgagc tgacggcgcg cgcgctcgcc gatgcggcga cgcgctggcc ggacgcgcac ttcacggtgc tcggcgacgc gggcctgatc gccgggcgcg cggccgcggc cggcgtcgac tgggggcgcg tgacggcggg cggcgagacg gggcatgttg ccgacgcgca tgtcgcagat gcgcacgtcg ccaacgcgca cgtcgccgtc gctcatcgcg cgcttgccgc gccggccgaa gcgggcaagc tgaatccggc gaacgggcgc tacgtgctcg cgctgctcga tgcggcgatc gacggcgcgc tcgccggcaa atacgacgcg atcgtcaccg cgccgctgca aaagagcacg atcaacgacg ccggcgtgcc gtttaccggc cataccgaat atctcgccga gcgcacgcac acgccgcgcg tcgtgatgat gcttgcgggc acgggcgagc ggccgctgcg cgtcgcgctc gcgacgacgc acctgccgct caaggacgtg gcggccgcgc tgacgatcga cggcctcgtc ggcacgctgt cgatcatcga tcgcgacctg cgcgaacgct tcggtctcgc cgcgccgcgc atcctcgtga cgggcctcaa tccgcatgcg ggcgagaacg gttatctcgg ccgcgaggaa atcgacgtga tcgagccggc gctcgaacgc gcgcgggcgg cgggcatcga cgcgcgcggc ccctatccgg ccgacacgct gttccagccg cgccacctcg agcacgcgga ttgcgtgctt gcgatgttcc acgatcaggg gctgccggtc ctcaagtacg cgacgttcgg cgaaggcatc aacgtcacgc tcggcctgcc gatcatccgc acgtcggtcg atcacggcac cgcgctcgat ctcgccggca cgggccgcgc cgatccgggc agcctcgtcg ccgcgatcga caccgccgtg acgatggcgc gccatcagcg cgcgggttga
Details for ButhA.00116.a.A1.GE32881
HARVESTED ON: 7/12/2011
SEQUENCED ON: 7/28/2011
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 22
PERCENT COVERAGE: 95
Validated AA Sequence
MARHRHGGVD RGDEAARIGA ARAGEIERGA VIDRRADDRQ AERDVDAFAE RRVLEDRQPL IVEHRKHAIR VLEVARLEQR VGRIGAARVD ARRPRAFERR LDHVDFLAAE ITVLARMRIE ARHEDARRGE TEAFAQVAID DRQRADEAVD RQRGRHVLER QVRRRERDAQ RPLARARKHH HDARRVRALG EIFGMAGKRH AGVVDRALLQ RRGDDRVVFA GERAVDRRIE QREHVAPVRR IQLARFGRRG KRAMSDGDVR VGDVRICDMR VGNMPRLAAR RHAPPVDAGR GRAPGDQARV AEHREVRVRP ARRRIGERAR RQLGADAGRL AGRDRDAQRL RTGGHRTRTL GLSFQ
Validated NT Sequence
ccgcgcgctg atggcgcgcc atcgtcacgg cggtgtcgat cgcggcgacg aggctgcccg gatcggcgcg gcccgtgccg gcgagatcga gcgcggtgcc gtgatcgacc gacgtgcgga tgatcggcag gccgagcgtg acgttgatgc cttcgccgaa cgtcgcgtac ttgaggaccg gcagcccctg atcgtggaac atcgcaagca cgcaatccgc gtgctcgagg tggcgcggct ggaacagcgt gtcggccgga taggggccgc gcgcgtcgat gcccgccgcc cgcgcgcgtt cgagcgccgg ctcgatcacg tcgatttcct cgcggccgag ataaccgttc tcgcccgcat gcggattgag gcccgtcacg aggatgcgcg gcgcggcgag accgaagcgt tcgcgcaggt cgcgatcgat gatcgacagc gtgccgacga ggccgtcgat cgtcagcgcg gccgccacgt ccttgagcgg caggtgcgtc gtcgcgagcg cgacgcgcag cggccgctcg cccgtgcccg caagcatcat cacgacgcgc ggcgtgtgcg tgcgctcggc gagatattcg gtatggccgg taaacggcac gccggcgtcg ttgatcgtgc tcttttgcag cggcgcggtg acgatcgcgt cgtatttgcc ggcgagcgcg ccgtcgatcg ccgcatcgag cagcgcgagc acgtagcgcc cgttcgccgg attcagcttg cccgcttcgg ccggcgcggc aagcgcgcga tgagcgacgg cgacgtgcgc gttggcgacg tgcgcatctg cgacatgcgc gtcggcaaca tgccccgtct cgccgcccgc cgtcacgcgc ccccagtcga cgccggccgc ggccgcgcgc ccggcgatca ggcccgcgtc gccgagcacc gtgaagtgcg cgtccggcca gcgcgtcgcc gcatcggcga gcgcgcgcgc cgtcagctcg gggccgacgc cggccggctc gccggtcgtg atcgcgatgc gcagcggctg cggacgggcg gacatcgaac caggaccctg ggtctgagct tccagggt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSARPQPLR IAITTGEPAG VGPELTARAL ADAATRWPDA HFTVLGDAGL IAGRAAAAGV DWGRVTAGGE TGHVADAHVA DAHVANAHVA VAHRALAAPA EAGKLNPANG RYVLALLDAA IDGALAGKYD AIVTAPLQKS TINDAGVPFT GHTEYLAERT HTPRVVMMLA GTGERPLRVA LATTHLPLKD VAAALTIDGL VGTLSIIDRD LRERFGLAAP RILVTGLNPH AGENGYLGRE EIDVIEPALE RARAAGIDAR GPYPADTLFQ PRHLEHADCV LAMFHDQGLP VLKYATFGEG INVTLGLPII RTSVDHGTAL DLAGTGRADP GSLVAAIDTA VTMARHQRAG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccgcccgt ccgcagccgc tgcgcatcgc gatcacgacc ggcgagccgg ccggcgtcgg ccccgagctg acggcgcgcg cgctcgccga tgcggcgacg cgctggccgg acgcgcactt cacggtgctc ggcgacgcgg gcctgatcgc cgggcgcgcg gccgcggccg gcgtcgactg ggggcgcgtg acggcgggcg gcgagacggg gcatgttgcc gacgcgcatg tcgcagatgc gcacgtcgcc aacgcgcacg tcgccgtcgc tcatcgcgcg cttgccgcgc cggccgaagc gggcaagctg aatccggcga acgggcgcta cgtgctcgcg ctgctcgatg cggcgatcga cggcgcgctc gccggcaaat acgacgcgat cgtcaccgcg ccgctgcaaa agagcacgat caacgacgcc ggcgtgccgt ttaccggcca taccgaatat ctcgccgagc gcacgcacac gccgcgcgtc gtgatgatgc ttgcgggcac gggcgagcgg ccgctgcgcg tcgcgctcgc gacgacgcac ctgccgctca aggacgtggc ggccgcgctg acgatcgacg gcctcgtcgg cacgctgtcg atcatcgatc gcgacctgcg cgaacgcttc ggtctcgccg cgccgcgcat cctcgtgacg ggcctcaatc cgcatgcggg cgagaacggt tatctcggcc gcgaggaaat cgacgtgatc gagccggcgc tcgaacgcgc gcgggcggcg ggcatcgacg cgcgcggccc ctatccggcc gacacgctgt tccagccgcg ccacctcgag cacgcggatt gcgtgcttgc gatgttccac gatcaggggc tgccggtcct caagtacgcg acgttcggcg aaggcatcaa cgtcacgctc ggcctgccga tcatccgcac gtcggtcgat cacggcaccg cgctcgatct cgccggcacg ggccgcgccg atccgggcag cctcgtcgcc gcgatcgaca ccgccgtgac gatggcgcgc catcagcgcg cgggttaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for ButhA.00116.a.A1.PS01184
PURIFICATION DATe: 8/29/2011
CONCENTRATION: 33.68mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 22
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MARHRHGGVD RGDEAARIGA ARAGEIERGA VIDRRADDRQ AERDVDAFAE RRVLEDRQPL IVEHRKHAIR VLEVARLEQR VGRIGAARVD ARRPRAFERR LDHVDFLAAE ITVLARMRIE ARHEDARRGE TEAFAQVAID DRQRADEAVD RQRGRHVLER QVRRRERDAQ RPLARARKHH HDARRVRALG EIFGMAGKRH AGVVDRALLQ RRGDDRVVFA GERAVDRRIE QREHVAPVRR IQLARFGRRG KRAMSDGDVR VGDVRICDMR VGNMPRLAAR RHAPPVDAGR GRAPGDQARV AEHREVRVRP ARRRIGERAR RQLGADAGRL AGRDRDAQRL RTGGHRTRTL GLSFQ
Validated NT Sequence
ccgcgcgctg atggcgcgcc atcgtcacgg cggtgtcgat cgcggcgacg aggctgcccg gatcggcgcg gcccgtgccg gcgagatcga gcgcggtgcc gtgatcgacc gacgtgcgga tgatcggcag gccgagcgtg acgttgatgc cttcgccgaa cgtcgcgtac ttgaggaccg gcagcccctg atcgtggaac atcgcaagca cgcaatccgc gtgctcgagg tggcgcggct ggaacagcgt gtcggccgga taggggccgc gcgcgtcgat gcccgccgcc cgcgcgcgtt cgagcgccgg ctcgatcacg tcgatttcct cgcggccgag ataaccgttc tcgcccgcat gcggattgag gcccgtcacg aggatgcgcg gcgcggcgag accgaagcgt tcgcgcaggt cgcgatcgat gatcgacagc gtgccgacga ggccgtcgat cgtcagcgcg gccgccacgt ccttgagcgg caggtgcgtc gtcgcgagcg cgacgcgcag cggccgctcg cccgtgcccg caagcatcat cacgacgcgc ggcgtgtgcg tgcgctcggc gagatattcg gtatggccgg taaacggcac gccggcgtcg ttgatcgtgc tcttttgcag cggcgcggtg acgatcgcgt cgtatttgcc ggcgagcgcg ccgtcgatcg ccgcatcgag cagcgcgagc acgtagcgcc cgttcgccgg attcagcttg cccgcttcgg ccggcgcggc aagcgcgcga tgagcgacgg cgacgtgcgc gttggcgacg tgcgcatctg cgacatgcgc gtcggcaaca tgccccgtct cgccgcccgc cgtcacgcgc ccccagtcga cgccggccgc ggccgcgcgc ccggcgatca ggcccgcgtc gccgagcacc gtgaagtgcg cgtccggcca gcgcgtcgcc gcatcggcga gcgcgcgcgc cgtcagctcg gggccgacgc cggccggctc gccggtcgtg atcgcgatgc gcagcggctg cggacgggcg gacatcgaac caggaccctg ggtctgagct tccagggt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSARPQPLR IAITTGEPAG VGPELTARAL ADAATRWPDA HFTVLGDAGL IAGRAAAAGV DWGRVTAGGE TGHVADAHVA DAHVANAHVA VAHRALAAPA EAGKLNPANG RYVLALLDAA IDGALAGKYD AIVTAPLQKS TINDAGVPFT GHTEYLAERT HTPRVVMMLA GTGERPLRVA LATTHLPLKD VAAALTIDGL VGTLSIIDRD LRERFGLAAP RILVTGLNPH AGENGYLGRE EIDVIEPALE RARAAGIDAR GPYPADTLFQ PRHLEHADCV LAMFHDQGLP VLKYATFGEG INVTLGLPII RTSVDHGTAL DLAGTGRADP GSLVAAIDTA VTMARHQRAG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCCGCCCGT CCGCAGCCGC TGCGCATCGC GATCACGACC GGCGAGCCGG CCGGCGTCGG CCCCGAGCTG ACGGCGCGCG CGCTCGCCGA TGCGGCGACG CGCTGGCCGG ACGCGCACTT CACGGTGCTC GGCGACGCGG GCCTGATCGC CGGGCGCGCG GCCGCGGCCG GCGTCGACTG GGGGCGCGTG ACGGCGGGCG GCGAGACGGG GCATGTTGCC GACGCGCATG TCGCAGATGC GCACGTCGCC AACGCGCACG TCGCCGTCGC TCATCGCGCG CTTGCCGCGC CGGCCGAAGC GGGCAAGCTG AATCCGGCGA ACGGGCGCTA CGTGCTCGCG CTGCTCGATG CGGCGATCGA CGGCGCGCTC GCCGGCAAAT ACGACGCGAT CGTCACCGCG CCGCTGCAAA AGAGCACGAT CAACGACGCC GGCGTGCCGT TTACCGGCCA TACCGAATAT CTCGCCGAGC GCACGCACAC GCCGCGCGTC GTGATGATGC TTGCGGGCAC GGGCGAGCGG CCGCTGCGCG TCGCGCTCGC GACGACGCAC CTGCCGCTCA AGGACGTGGC GGCCGCGCTG ACGATCGACG GCCTCGTCGG CACGCTGTCG ATCATCGATC GCGACCTGCG CGAACGCTTC GGTCTCGCCG CGCCGCGCAT CCTCGTGACG GGCCTCAATC CGCATGCGGG CGAGAACGGT TATCTCGGCC GCGAGGAAAT CGACGTGATC GAGCCGGCGC TCGAACGCGC GCGGGCGGCG GGCATCGACG CGCGCGGCCC CTATCCGGCC GACACGCTGT TCCAGCCGCG CCACCTCGAG CACGCGGATT GCGTGCTTGC GATGTTCCAC GATCAGGGGC TGCCGGTCCT CAAGTACGCG ACGTTCGGCG AAGGCATCAA CGTCACGCTC GGCCTGCCGA TCATCCGCAC GTCGGTCGAT CACGGCACCG CGCTCGATCT CGCCGGCACG GGCCGCGCCG ATCCGGGCAG CCTCGTCGCC GCGATCGACA CCGCCGTGAC GATGGCGCGC CATCAGCGCG CGGGTTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA