ButhA.00113.a

3-oxoacyl-(Acyl-carrier-protein) synthase II

CENTER ID: ButhA.00113.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00113.a.A1.GE32417 Full length( ButhA.00113.a ) 1 421
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00113.a.A1.PW32337 Full length( ButhA.00113.a ) 1 421

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.4510
RefSeq: YP_442256.1
UniProt: Q2SXU3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSFNGKEVTV SRRRVVVTGL GLISPVGNNV ADGWANLVAG KSGIVNITKF DATNFSTRFA GEVKGFNVED YIPAKEARHM DTFIHYGIAA GMQAMQDSGF QVTDENAERV GVVVGSGIGG LPMIEVTQTE LLNRGPRRIS PFFVPASIIN MVSGHLSIKF GIKGPNLAIV TACTTGLHCI GEAARLIEYG DADVMIAGGA EATVSPLGIG GFAAARALSQ RNDDPATASR PWDKDRDGFV LGEGAGVMVL EEYEHAKARG AKIYAEIGGY GMSGDAYHMT APLEDGDGAR RCMLAALRNA GINVDQVNYL NAHGTSTPLG DVAETVAIKR AFGDHAKQIV VNSTKSMTGH LLGGSGGLES VFTVLAVHNQ VSPPTINIFN QDPACDLDYC ANEAREMKID VALKNSFGFG GTNGTLVFKR V
NT Sequence
TTGTCATTCA ATGGAAAAGA GGTTACCGTG AGCCGCCGTC GTGTTGTTGT TACAGGCCTT GGGCTGATTT CGCCTGTTGG CAATAATGTT GCCGACGGCT GGGCCAATCT CGTGGCCGGC AAGTCCGGCA TCGTCAATAT CACGAAGTTC GACGCGACGA ACTTCTCGAC CCGCTTCGCA GGCGAGGTGA AGGGCTTCAA CGTCGAGGAT TACATCCCGG CGAAGGAAGC GCGCCACATG GATACGTTCA TCCATTACGG GATCGCGGCC GGCATGCAGG CGATGCAGGA CAGCGGCTTC CAGGTCACCG ACGAAAACGC GGAGCGCGTC GGCGTCGTGG TCGGCTCGGG CATCGGCGGG CTGCCGATGA TCGAGGTCAC GCAGACCGAG CTGCTTAACC GCGGCCCGCG CCGGATCTCG CCGTTCTTCG TGCCGGCGTC GATCATCAAT ATGGTGTCCG GCCATCTGTC GATCAAGTTC GGCATCAAGG GCCCGAATCT GGCGATCGTC ACCGCTTGCA CGACCGGCCT GCACTGCATC GGCGAAGCTG CGCGCCTCAT CGAATACGGC GACGCGGACG TGATGATCGC GGGCGGCGCG GAGGCGACGG TGTCGCCGCT CGGCATCGGC GGCTTCGCGG CGGCGCGCGC GCTGTCGCAG CGCAACGACG ATCCGGCGAC CGCGAGCCGC CCGTGGGACA AGGACCGCGA CGGCTTCGTG CTCGGCGAAG GCGCGGGCGT GATGGTGCTC GAGGAGTACG AGCACGCGAA GGCGCGGGGC GCGAAGATCT ACGCGGAAAT CGGGGGCTAC GGGATGAGCG GCGACGCGTA TCACATGACG GCTCCGCTCG AGGATGGCGA CGGTGCGCGA CGCTGCATGC TGGCCGCGCT GCGCAACGCC GGCATCAACG TCGATCAGGT CAACTACCTG AACGCGCACG GCACGTCGAC GCCGCTTGGC GACGTCGCGG AAACGGTCGC GATCAAGCGG GCGTTCGGCG ATCACGCGAA GCAAATCGTC GTCAACTCGA CGAAGTCGAT GACGGGCCAC CTGCTCGGCG GGTCGGGCGG GCTCGAATCG GTGTTCACGG TCCTCGCCGT CCACAACCAG GTGTCGCCGC CGACGATCAA CATCTTCAAC CAGGATCCCG CGTGCGATCT GGATTATTGC GCGAACGAAG CGCGCGAGAT GAAGATCGAT GTCGCGTTGA AGAACTCCTT CGGCTTCGGC GGGACTAACG GCACGCTGGT CTTCAAGCGC GTC
Details for ButhA.00113.a.A1.GE32417
HARVESTED ON: 11/8/2011
SEQUENCED ON: 11/11/2011
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MLSFNGKEVT VSRRRVVVTG LGLISPVGNN VADGWANLVA GKSGIVNITK FDATNFSTRF AGEVKGFNVE DYIPAKEARH MDTFIHYGIA AGMQAMQDSG FQVTDENAER VGVVVGSGIG GLPMIEVTQT ELLNRGPRRI SPFFVPASII NMVSGHLSIK FGIKGPNLAI VTACTTGLHC IGEAARLIEY GDADVMIAGG AEATVSPLGI GGFAAARALS QRNDDPATAS RPWDKDRDGF VLGEGAGVMV LEEYEHAKAR GAKIYAEIGG YGMSGDAYHM TAPLEDGDGA RRCMLAALRN AGINVDQVNY LNAHGTSTPL GDVAETVAIK RAFGDHAKQI VVNSTKSMTG HLLGGSGGLE SVFTVLAVHN QVSPPTINIF NQDPACDLDY CANEAREMKI DVALKNSFGF GGTNGTLVFK R
Validated NT Sequence
gacgcgcttg aagaccagcg tgccgttagt cccgccgaag ccgaaggagt tcttcaacgc gacatcgatc ttcatctcgc gcgcttcgtt cgcgcaataa tccagatcgc acgcgggatc ctggttgaag atgttgatcg tcggcggcga cacctggttg tggacggcga ggaccgtgaa caccgattcg agcccgcccg acccgccgag caggtggccc gtcatcgact tcgtcgagtt gacgacgatt tgcttcgcgt gatcgccgaa cgcccgcttg atcgcgaccg tttccgcgac gtcgccaagc ggcgtcgacg tgccgtgcgc gttcaggtag ttgacctgat cgacgttgat gccggcgttg cgcagcgcgg ccagcatgca gcgtcgcgca ccgtcgccat cctcgagcgg agccgtcatg tgatacgcgt cgccgctcat cccgtagccc ccgatttccg cgtagatctt cgcgccccgc gccttcgcgt gctcgtactc ctcgagcacc atcacgcccg cgccttcgcc gagcacgaag ccgtcgcggt ccttgtccca cgggcggctc gcggtcgccg gatcgtcgtt gcgctgcgac agcgcgcgcg ccgccgcgaa gccgccgatg ccgagcggcg acaccgtcgc ctccgcgccg cccgcgatca tcacgtccgc gtcgccgtat tcgatgaggc gcgcagcttc gccgatgcag tgcaggccgg tcgtgcaagc ggtgacgatc gccagattcg ggcccttgat gccgaacttg atcgacagat ggccggacac catattgatg atcgacgccg gcacgaagaa cggcgagatc cggcgcgggc cgcggttaag cagctcggtc tgcgtgacct cgatcatcgg cagcccgccg atgcccgagc cgaccacgac gccgacgcgc tccgcgtttt cgtcggtgac ctggaagccg ctgtcctgca tcgcctgcat gccggccgcg atcccgtaat ggatgaacgt atccatgtgg cgcgcttcct tcgccgggat gtaatcctcg acgttgaagc ccttcacctc gcctgcgaag cgggtcgaga agttcgtcgc gtcgaacttc gtgatattga cgatgccgga cttgccggcc acgagattgg cccagccgtc ggcaacatta ttgccaacag gcgaaatcag cccaaggcct gtaacaacaa cacgacggcg gctcacggta acctcttttc cattgaatga caacatcgaa ccaggaccct gggtctgagc ttccagggta cc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLSFNGKEV TVSRRRVVVT GLGLISPVGN NVADGWANLV AGKSGIVNIT KFDATNFSTR FAGEVKGFNV EDYIPAKEAR HMDTFIHYGI AAGMQAMQDS GFQVTDENAE RVGVVVGSGI GGLPMIEVTQ TELLNRGPRR ISPFFVPASI INMVSGHLSI KFGIKGPNLA IVTACTTGLH CIGEAARLIE YGDADVMIAG GAEATVSPLG IGGFAAARAL SQRNDDPATA SRPWDKDRDG FVLGEGAGVM VLEEYEHAKA RGAKIYAEIG GYGMSGDAYH MTAPLEDGDG ARRCMLAALR NAGINVDQVN YLNAHGTSTP LGDVAETVAI KRAFGDHAKQ IVVNSTKSMT GHLLGGSGGL ESVFTVLAVH NQVSPPTINI FNQDPACDLD YCANEAREMK IDVALKNSFG FGGTNGTLVF KRV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgtcattc aatggaaaag aggttaccgt gagccgccgt cgtgttgttg ttacaggcct tgggctgatt tcgcctgttg gcaataatgt tgccgacggc tgggccaatc tcgtggccgg caagtccggc atcgtcaata tcacgaagtt cgacgcgacg aacttctcga cccgcttcgc aggcgaggtg aagggcttca acgtcgagga ttacatcccg gcgaaggaag cgcgccacat ggatacgttc atccattacg ggatcgcggc cggcatgcag gcgatgcagg acagcggctt ccaggtcacc gacgaaaacg cggagcgcgt cggcgtcgtg gtcggctcgg gcatcggcgg gctgccgatg atcgaggtca cgcagaccga gctgcttaac cgcggcccgc gccggatctc gccgttcttc gtgccggcgt cgatcatcaa tatggtgtcc ggccatctgt cgatcaagtt cggcatcaag ggcccgaatc tggcgatcgt caccgcttgc acgaccggcc tgcactgcat cggcgaagct gcgcgcctca tcgaatacgg cgacgcggac gtgatgatcg cgggcggcgc ggaggcgacg gtgtcgccgc tcggcatcgg cggcttcgcg gcggcgcgcg cgctgtcgca gcgcaacgac gatccggcga ccgcgagccg cccgtgggac aaggaccgcg acggcttcgt gctcggcgaa ggcgcgggcg tgatggtgct cgaggagtac gagcacgcga aggcgcgggg cgcgaagatc tacgcggaaa tcgggggcta cgggatgagc ggcgacgcgt atcacatgac ggctccgctc gaggatggcg acggtgcgcg acgctgcatg ctggccgcgc tgcgcaacgc cggcatcaac gtcgatcagg tcaactacct gaacgcgcac ggcacgtcga cgccgcttgg cgacgtcgcg gaaacggtcg cgatcaagcg ggcgttcggc gatcacgcga agcaaatcgt cgtcaactcg acgaagtcga tgacgggcca cctgctcggc gggtcgggcg ggctcgaatc ggtgttcacg gtcctcgccg tccacaacca ggtgtcgccg ccgacgatca acatcttcaa ccaggatccc gcgtgcgatc tggattattg cgcgaacgaa gcgcgcgaga tgaagatcga tgtcgcgttg aagaactcct tcggcttcgg cgggactaac ggcacgctgg tcttcaagcg cgtctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for ButhA.00113.a.A1.PW32337
PURIFICATION DATe: 1/14/2012
CONCENTRATION: 31.4mg/ml
OBSERVED MW: 43kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MLSFNGKEVT VSRRRVVVTG LGLISPVGNN VADGWANLVA GKSGIVNITK FDATNFSTRF AGEVKGFNVE DYIPAKEARH MDTFIHYGIA AGMQAMQDSG FQVTDENAER VGVVVGSGIG GLPMIEVTQT ELLNRGPRRI SPFFVPASII NMVSGHLSIK FGIKGPNLAI VTACTTGLHC IGEAARLIEY GDADVMIAGG AEATVSPLGI GGFAAARALS QRNDDPATAS RPWDKDRDGF VLGEGAGVMV LEEYEHAKAR GAKIYAEIGG YGMSGDAYHM TAPLEDGDGA RRCMLAALRN AGINVDQVNY LNAHGTSTPL GDVAETVAIK RAFGDHAKQI VVNSTKSMTG HLLGGSGGLE SVFTVLAVHN QVSPPTINIF NQDPACDLDY CANEAREMKI DVALKNSFGF GGTNGTLVFK R
Validated NT Sequence
gacgcgcttg aagaccagcg tgccgttagt cccgccgaag ccgaaggagt tcttcaacgc gacatcgatc ttcatctcgc gcgcttcgtt cgcgcaataa tccagatcgc acgcgggatc ctggttgaag atgttgatcg tcggcggcga cacctggttg tggacggcga ggaccgtgaa caccgattcg agcccgcccg acccgccgag caggtggccc gtcatcgact tcgtcgagtt gacgacgatt tgcttcgcgt gatcgccgaa cgcccgcttg atcgcgaccg tttccgcgac gtcgccaagc ggcgtcgacg tgccgtgcgc gttcaggtag ttgacctgat cgacgttgat gccggcgttg cgcagcgcgg ccagcatgca gcgtcgcgca ccgtcgccat cctcgagcgg agccgtcatg tgatacgcgt cgccgctcat cccgtagccc ccgatttccg cgtagatctt cgcgccccgc gccttcgcgt gctcgtactc ctcgagcacc atcacgcccg cgccttcgcc gagcacgaag ccgtcgcggt ccttgtccca cgggcggctc gcggtcgccg gatcgtcgtt gcgctgcgac agcgcgcgcg ccgccgcgaa gccgccgatg ccgagcggcg acaccgtcgc ctccgcgccg cccgcgatca tcacgtccgc gtcgccgtat tcgatgaggc gcgcagcttc gccgatgcag tgcaggccgg tcgtgcaagc ggtgacgatc gccagattcg ggcccttgat gccgaacttg atcgacagat ggccggacac catattgatg atcgacgccg gcacgaagaa cggcgagatc cggcgcgggc cgcggttaag cagctcggtc tgcgtgacct cgatcatcgg cagcccgccg atgcccgagc cgaccacgac gccgacgcgc tccgcgtttt cgtcggtgac ctggaagccg ctgtcctgca tcgcctgcat gccggccgcg atcccgtaat ggatgaacgt atccatgtgg cgcgcttcct tcgccgggat gtaatcctcg acgttgaagc ccttcacctc gcctgcgaag cgggtcgaga agttcgtcgc gtcgaacttc gtgatattga cgatgccgga cttgccggcc acgagattgg cccagccgtc ggcaacatta ttgccaacag gcgaaatcag cccaaggcct gtaacaacaa cacgacggcg gctcacggta acctcttttc cattgaatga caacatcgaa ccaggaccct gggtctgagc ttccagggta cc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLSFNGKEV TVSRRRVVVT GLGLISPVGN NVADGWANLV AGKSGIVNIT KFDATNFSTR FAGEVKGFNV EDYIPAKEAR HMDTFIHYGI AAGMQAMQDS GFQVTDENAE RVGVVVGSGI GGLPMIEVTQ TELLNRGPRR ISPFFVPASI INMVSGHLSI KFGIKGPNLA IVTACTTGLH CIGEAARLIE YGDADVMIAG GAEATVSPLG IGGFAAARAL SQRNDDPATA SRPWDKDRDG FVLGEGAGVM VLEEYEHAKA RGAKIYAEIG GYGMSGDAYH MTAPLEDGDG ARRCMLAALR NAGINVDQVN YLNAHGTSTP LGDVAETVAI KRAFGDHAKQ IVVNSTKSMT GHLLGGSGGL ESVFTVLAVH NQVSPPTINI FNQDPACDLD YCANEAREMK IDVALKNSFG FGGTNGTLVF KRV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGTCATTC AATGGAAAAG AGGTTACCGT GAGCCGCCGT CGTGTTGTTG TTACAGGCCT TGGGCTGATT TCGCCTGTTG GCAATAATGT TGCCGACGGC TGGGCCAATC TCGTGGCCGG CAAGTCCGGC ATCGTCAATA TCACGAAGTT CGACGCGACG AACTTCTCGA CCCGCTTCGC AGGCGAGGTG AAGGGCTTCA ACGTCGAGGA TTACATCCCG GCGAAGGAAG CGCGCCACAT GGATACGTTC ATCCATTACG GGATCGCGGC CGGCATGCAG GCGATGCAGG ACAGCGGCTT CCAGGTCACC GACGAAAACG CGGAGCGCGT CGGCGTCGTG GTCGGCTCGG GCATCGGCGG GCTGCCGATG ATCGAGGTCA CGCAGACCGA GCTGCTTAAC CGCGGCCCGC GCCGGATCTC GCCGTTCTTC GTGCCGGCGT CGATCATCAA TATGGTGTCC GGCCATCTGT CGATCAAGTT CGGCATCAAG GGCCCGAATC TGGCGATCGT CACCGCTTGC ACGACCGGCC TGCACTGCAT CGGCGAAGCT GCGCGCCTCA TCGAATACGG CGACGCGGAC GTGATGATCG CGGGCGGCGC GGAGGCGACG GTGTCGCCGC TCGGCATCGG CGGCTTCGCG GCGGCGCGCG CGCTGTCGCA GCGCAACGAC GATCCGGCGA CCGCGAGCCG CCCGTGGGAC AAGGACCGCG ACGGCTTCGT GCTCGGCGAA GGCGCGGGCG TGATGGTGCT CGAGGAGTAC GAGCACGCGA AGGCGCGGGG CGCGAAGATC TACGCGGAAA TCGGGGGCTA CGGGATGAGC GGCGACGCGT ATCACATGAC GGCTCCGCTC GAGGATGGCG ACGGTGCGCG ACGCTGCATG CTGGCCGCGC TGCGCAACGC CGGCATCAAC GTCGATCAGG TCAACTACCT GAACGCGCAC GGCACGTCGA CGCCGCTTGG CGACGTCGCG GAAACGGTCG CGATCAAGCG GGCGTTCGGC GATCACGCGA AGCAAATCGT CGTCAACTCG ACGAAGTCGA TGACGGGCCA CCTGCTCGGC GGGTCGGGCG GGCTCGAATC GGTGTTCACG GTCCTCGCCG TCCACAACCA GGTGTCGCCG CCGACGATCA ACATCTTCAA CCAGGATCCC GCGTGCGATC TGGATTATTG CGCGAACGAA GCGCGCGAGA TGAAGATCGA TGTCGCGTTG AAGAACTCCT TCGGCTTCGG CGGGACTAAC GGCACGCTGG TCTTCAAGCG CGTCTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA