ButhA.00112.a

3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11) (Ketopantoate hydroxymethyltransferase) (KPHMT) (BTH_I1311) (panB)

CENTER ID: ButhA.00112.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: in PDB
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
ButhA.00112.a.A1.GE32721 full length 1 271
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
ButhA.00112.a.A1.PW33325 full length 1 271

Structures

3VAV
DEPOSITED: 12/29/2011
DETERMINATION: XRay
CLONE: ButhA.00112.a.A1.GE32721
PROTEIN: ButhA.00112.a.A1.PW33325

Publications by SSGCID

Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_129046
PATRIC ID: fig|271848.6.peg.4078
RefSeq: YP_441858.1
UniProt: Q2SYZ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTYLQESSRP AVTVPKLQAM REAGEKIAML TCYDASFAAL LDRANVDVQL IGDSLGNVLQ GQTTTLPVTL DDIAYHTACV ARAQPRALIV ADLPFGTYGT PADAFASAVK LMRAGAQMVK FEGGEWLAET VRFLVERAVP VCAHVGLTPQ SVHAFGGFKV QGKTEAGAAQ LLRDARAVEE AGAQLIVLEA VPTLVAAEVT RELSIPTIGI GAGAECSGQV LVLHDMLGVF PGKRPRFVKD FMQGQPSIFA AVEAYVRAVK DGSFPGPEHS F
NT Sequence
atgacctacc ttcaggaatc cagccgaccg gccgtgacgg tgcccaagct gcaggcgatg cgtgaagccg gcgagaagat cgccatgctc acctgctacg acgcgagctt cgccgccctc ctcgatcgcg cgaacgtcga cgtgcaactg atcggcgatt cgctcggcaa cgtgctgcaa ggccagacga cgacgctgcc cgtcacgctc gacgacatcg cgtaccacac cgcctgcgtc gcgcgcgcgc agccgcgcgc gctcatcgtc gcggacctgc cgttcggcac ctacggcacg cccgccgacg cgttcgcgag cgcggtgaag ctgatgcgcg cgggcgcgca gatggtcaag ttcgaaggcg gcgaatggct cgccgaaacg gtgcgcttcc tcgtcgagcg agcggtgccc gtgtgcgcgc acgtcggcct cacgccgcaa tcggtgcacg cgttcggcgg cttcaaggta cagggaaaga cggaagcggg cgccgcgcaa ttgctgcgcg acgcgcgcgc ggtcgaagaa gcgggcgcgc agctcatcgt gctcgaggcc gtgccgacgc tcgtcgcggc ggaagtcacg cgcgagctgt cgatcccgac gatcggcatc ggcgcgggtg cggaatgttc cggccaggtc ctcgtgctgc acgacatgct cggcgtgttc cccggcaaac gccctcgctt cgtcaaggat ttcatgcagg gacagccgag catcttcgcg gccgtcgagg cttacgtgcg cgcggtgaag gacggcagct tccccgggcc cgagcattcg ttctga
Details for ButhA.00112.a.A1.GE32721
HARVESTED ON: 6/20/2011
SEQUENCED ON: 7/14/2011
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTYLQESSR PAVTVPKLQA MREAGEKIAM LTCYDASFAA LLDRANVDVQ LIGDSLGNVL QGQTTTLPVT LDDIAYHTAC VARAQPRALI VADLPFGTYG TPADAFASAV KLMRAGAQMV KFEGGEWLAE TVRFLVERAV PVCAHVGLTP QSVHAFGGFK VQGKTEAGAA QLLRDARAVE EAGAQLIVLE AVPTLVAAEV TRELSIPTIG IGAGAECSGQ VLVLHDMLGV FPGKRPRFVK DFMQGQPSIF AAVEAYVRAV KDGSFPGPEH SF
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacct accttcagga atccagccga ccggccgtga cggtgcccaa gctgcaggcg atgcgtgaag ccggcgagaa gatcgccatg ctcacctgct acgacgcgag cttcgccgcc ctcctcgatc gcgcgaacgt cgacgtgcaa ctgatcggcg attcgctcgg caacgtgctg caaggccaga cgacgacgct gcccgtcacg ctcgacgaca tcgcgtacca caccgcctgc gtcgcgcgcg cgcagccgcg cgcgctcatc gtcgcggacc tgccgttcgg cacctacggc acgcccgccg acgcgttcgc gagcgcggtg aagctgatgc gcgcgggcgc gcagatggtc aagttcgaag gcggcgaatg gctcgccgaa acggtgcgct tcctcgtcga gcgagcggtg cccgtgtgcg cgcacgtcgg cctcacgccg caatcggtgc acgcgttcgg cggcttcaag gtacagggaa agacggaagc gggcgccgcg caattgctgc gcgacgcgcg cgcggtcgaa gaagcgggcg cgcagctcat cgtgctcgag gccgtgccga cgctcgtcgc ggcggaagtc acgcgcgagc tgtcgatccc gacgatcggc atcggcgcgg gtgcggaatg ttccggccag gtcctcgtgc tgcacgacat gctcggcgtg ttccccggca aacgccctcg cttcgtcaag gatttcatgc agggacagcc gagcatcttc gcggccgtcg aggcttacgt gcgcgcggtg aaggacggca gcttccccgg gcccgagcat tcgttctaat a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTYLQESSR PAVTVPKLQA MREAGEKIAM LTCYDASFAA LLDRANVDVQ LIGDSLGNVL QGQTTTLPVT LDDIAYHTAC VARAQPRALI VADLPFGTYG TPADAFASAV KLMRAGAQMV KFEGGEWLAE TVRFLVERAV PVCAHVGLTP QSVHAFGGFK VQGKTEAGAA QLLRDARAVE EAGAQLIVLE AVPTLVAAEV TRELSIPTIG IGAGAECSGQ VLVLHDMLGV FPGKRPRFVK DFMQGQPSIF AAVEAYVRAV KDGSFPGPEH SF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacctacctt caggaatcca gccgaccggc cgtgacggtg cccaagctgc aggcgatgcg tgaagccggc gagaagatcg ccatgctcac ctgctacgac gcgagcttcg ccgccctcct cgatcgcgcg aacgtcgacg tgcaactgat cggcgattcg ctcggcaacg tgctgcaagg ccagacgacg acgctgcccg tcacgctcga cgacatcgcg taccacaccg cctgcgtcgc gcgcgcgcag ccgcgcgcgc tcatcgtcgc ggacctgccg ttcggcacct acggcacgcc cgccgacgcg ttcgcgagcg cggtgaagct gatgcgcgcg ggcgcgcaga tggtcaagtt cgaaggcggc gaatggctcg ccgaaacggt gcgcttcctc gtcgagcgag cggtgcccgt gtgcgcgcac gtcggcctca cgccgcaatc ggtgcacgcg ttcggcggct tcaaggtaca gggaaagacg gaagcgggcg ccgcgcaatt gctgcgcgac gcgcgcgcgg tcgaagaagc gggcgcgcag ctcatcgtgc tcgaggccgt gccgacgctc gtcgcggcgg aagtcacgcg cgagctgtcg atcccgacga tcggcatcgg cgcgggtgcg gaatgttccg gccaggtcct cgtgctgcac gacatgctcg gcgtgttccc cggcaaacgc cctcgcttcg tcaaggattt catgcaggga cagccgagca tcttcgcggc cgtcgaggct tacgtgcgcg cggtgaagga cggcagcttc cccgggcccg agcattcgtt ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for ButhA.00112.a.A1.PW33325
PURIFICATION DATe: 8/12/2011
CONCENTRATION: 38.63mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTYLQESSR PAVTVPKLQA MREAGEKIAM LTCYDASFAA LLDRANVDVQ LIGDSLGNVL QGQTTTLPVT LDDIAYHTAC VARAQPRALI VADLPFGTYG TPADAFASAV KLMRAGAQMV KFEGGEWLAE TVRFLVERAV PVCAHVGLTP QSVHAFGGFK VQGKTEAGAA QLLRDARAVE EAGAQLIVLE AVPTLVAAEV TRELSIPTIG IGAGAECSGQ VLVLHDMLGV FPGKRPRFVK DFMQGQPSIF AAVEAYVRAV KDGSFPGPEH SF
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacct accttcagga atccagccga ccggccgtga cggtgcccaa gctgcaggcg atgcgtgaag ccggcgagaa gatcgccatg ctcacctgct acgacgcgag cttcgccgcc ctcctcgatc gcgcgaacgt cgacgtgcaa ctgatcggcg attcgctcgg caacgtgctg caaggccaga cgacgacgct gcccgtcacg ctcgacgaca tcgcgtacca caccgcctgc gtcgcgcgcg cgcagccgcg cgcgctcatc gtcgcggacc tgccgttcgg cacctacggc acgcccgccg acgcgttcgc gagcgcggtg aagctgatgc gcgcgggcgc gcagatggtc aagttcgaag gcggcgaatg gctcgccgaa acggtgcgct tcctcgtcga gcgagcggtg cccgtgtgcg cgcacgtcgg cctcacgccg caatcggtgc acgcgttcgg cggcttcaag gtacagggaa agacggaagc gggcgccgcg caattgctgc gcgacgcgcg cgcggtcgaa gaagcgggcg cgcagctcat cgtgctcgag gccgtgccga cgctcgtcgc ggcggaagtc acgcgcgagc tgtcgatccc gacgatcggc atcggcgcgg gtgcggaatg ttccggccag gtcctcgtgc tgcacgacat gctcggcgtg ttccccggca aacgccctcg cttcgtcaag gatttcatgc agggacagcc gagcatcttc gcggccgtcg aggcttacgt gcgcgcggtg aaggacggca gcttccccgg gcccgagcat tcgttctaat a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTYLQESSR PAVTVPKLQA MREAGEKIAM LTCYDASFAA LLDRANVDVQ LIGDSLGNVL QGQTTTLPVT LDDIAYHTAC VARAQPRALI VADLPFGTYG TPADAFASAV KLMRAGAQMV KFEGGEWLAE TVRFLVERAV PVCAHVGLTP QSVHAFGGFK VQGKTEAGAA QLLRDARAVE EAGAQLIVLE AVPTLVAAEV TRELSIPTIG IGAGAECSGQ VLVLHDMLGV FPGKRPRFVK DFMQGQPSIF AAVEAYVRAV KDGSFPGPEH SF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCTACCTT CAGGAATCCA GCCGACCGGC CGTGACGGTG CCCAAGCTGC AGGCGATGCG TGAAGCCGGC GAGAAGATCG CCATGCTCAC CTGCTACGAC GCGAGCTTCG CCGCCCTCCT CGATCGCGCG AACGTCGACG TGCAACTGAT CGGCGATTCG CTCGGCAACG TGCTGCAAGG CCAGACGACG ACGCTGCCCG TCACGCTCGA CGACATCGCG TACCACACCG CCTGCGTCGC GCGCGCGCAG CCGCGCGCGC TCATCGTCGC GGACCTGCCG TTCGGCACCT ACGGCACGCC CGCCGACGCG TTCGCGAGCG CGGTGAAGCT GATGCGCGCG GGCGCGCAGA TGGTCAAGTT CGAAGGCGGC GAATGGCTCG CCGAAACGGT GCGCTTCCTC GTCGAGCGAG CGGTGCCCGT GTGCGCGCAC GTCGGCCTCA CGCCGCAATC GGTGCACGCG TTCGGCGGCT TCAAGGTACA GGGAAAGACG GAAGCGGGCG CCGCGCAATT GCTGCGCGAC GCGCGCGCGG TCGAAGAAGC GGGCGCGCAG CTCATCGTGC TCGAGGCCGT GCCGACGCTC GTCGCGGCGG AAGTCACGCG CGAGCTGTCG ATCCCGACGA TCGGCATCGG CGCGGGTGCG GAATGTTCCG GCCAGGTCCT CGTGCTGCAC GACATGCTCG GCGTGTTCCC CGGCAAACGC CCTCGCTTCG TCAAGGATTT CATGCAGGGA CAGCCGAGCA TCTTCGCGGC CGTCGAGGCT TACGTGCGCG CGGTGAAGGA CGGCAGCTTC CCCGGGCCCG AGCATTCGTT CTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA