SSGCID: Target Page

ButhA.00111.a

Glutamine synthetase (EC 6.3.1.2) (BTH_I1844) (glnA)

CENTER ID:	ButhA.00111.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00111.a.A1.GE32720	full length		1	471

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00111.a.A1.PW33324	full length		1	471

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.4647
RefSeq:	YP_442375.1
UniProt:	Q2SXH4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSKTVADVMQ LAKDEDVKFV DFRFTDTRGK EQHVSVPVSA FGEDKFESGH AFDGSSIAGW KGIEASDMLL MPDPNAAFID PFYEESTLVL TCDVVEPADG KGYERDPRSL AKRAEAYLKS TGIGDTAYFG PEPEFFIFDS VQWNTDMSGC FVKINSEEAP WSSAKEFEGG NTGHRPGVKG GYFPVAPVDQ FQDMRSEMCL LLEQIGIPVE VHHHEVAGQG QNEIGTKFST LLQRADWTQW SKYIIHNVAH SYGKTATFMP KPVVGDNGSG MHVHQSIWKD GQNLFAGNGY AGLSETALFY IGGIIKHARA LNAITNPTTN SYKRLVPHFE APVKLAYSAR NRSASIRIPH VSNPKGRRIE TRFPDPMANP YLCFSALMMA GLDGIQNKIH PGEAADKNLY DLPPEEDAKI PTVCAGLDQA LEALDKDREF LTRGGVFTDA MIDAYLGLKE QELAKFRMTT HPIEFEMYYS L

NT Sequence

atgagtaaaa ccgtcgccga cgtcatgcag ctcgcgaagg acgaggacgt caagtttgtc gatttccgct tcacggacac gcgcggcaag gagcaacacg tgtcggtgcc ggtgtcggct ttcggcgagg acaagttcga aagcggccac gcgttcgacg gctcgtcgat cgcgggctgg aagggcatcg aggcgtcgga catgctgctc atgccggacc cgaacgccgc cttcatcgac ccgttctacg aagagtcgac cctcgtgctg acctgcgacg tcgtcgagcc cgccgacggc aagggctacg agcgcgaccc gcgctcgctc gcgaagcgcg ccgaggcata cctgaagagc acgggcatcg gcgacaccgc ctacttcggt ccggagccgg aattcttcat tttcgactcg gtgcagtgga acaccgacat gtcgggctgc ttcgtgaaga tcaactcgga agaagcgccg tggtcgtcgg cgaaggaatt cgaaggcggc aacacgggcc accgtccggg cgtgaagggc ggctacttcc ctgtcgcgcc ggtcgaccag ttccaggaca tgcgctcgga aatgtgcctg ctgctcgagc agatcggcat tccggtcgaa gtgcaccacc acgaagtcgc gggccagggc cagaacgaaa tcggcacgaa gttctcgacg ctcctgcagc gcgcggactg gacgcaatgg tcgaagtaca tcatccataa cgtcgcgcat tcgtacggca agacggcgac gttcatgccg aagcccgtcg tcggcgacaa cggctcgggc atgcacgtgc accagtcgat ctggaaggac ggccagaacc tgttcgcggg caatggctac gccggcctgt cggaaacggc gctgttctac atcggcggca tcatcaagca cgcgcgcgcg ctgaacgcga tcacgaaccc gacgacgaac tcgtacaagc gtctcgtgcc gcacttcgaa gcgcccgtga agctcgctta ctcggcgcgc aaccgctcgg cgtcgatccg cattccgcac gtgtcgaacc cgaagggccg ccgcatcgaa acgcgcttcc cggacccgat ggcgaacccg tacctgtgct tctcggcgct gatgatggcg ggtctcgacg gcatccagaa caagatccac ccgggcgagg ctgcggacaa gaacctgtac gacctgccgc cggaagagga tgcaaagatc ccgaccgtgt gcgccggcct cgaccaggcg ctcgaggcgc tcgacaagga ccgcgagttc ctgacccgcg gcggcgtgtt cacggacgcg atgatcgacg cgtacctggg cctgaaggaa caggagctcg cgaagttccg catgacgacg catccgatcg agttcgagat gtactactcg ctgtaa

Details for ButhA.00111.a.A1.GE32720

HARVESTED ON:	6/20/2011
SEQUENCED ON:	7/14/2011
EXPECTED MW:	54kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	18
PERCENT COVERAGE:	67

Validated AA Sequence

MRRHAELREL LFLQAQVRVD HRVREHAAAG QELAVLVERL ERLVEAGAHG RDLCILFRRQ VVQVLVRSLA RVDLVLDAVE TRHHQRREAQ VRVRHRVREA RFDAAALRVR HVRNADRRRA VARRVSELHG RFEVRHETLV RVRRRVRDRV QRARVLDDAA DVEQRRFRQA GVAIAREQVL AVLPDRLXHV HARAVVADDG LRHERRRLAV RMRDVMDDVL RPLRPVRALQ ERRELRADFV LALARDFVVV HFDRNADLLE QQAHFRAHVL ELVDRRDRXS SRPSRPDGGP CCRXSNSFAD DHGASSELIF TKQPDMXXXH CTESKMKNSG SGPK

Validated NT Sequence

tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgagtaa aaccgtcgcc gacgtcatgc agctcgcgaa ggacgaggac gtcaagtttg tcgatttccg cttcacggac acgcgcggca aggagcaaca cgtgtcggtg ccggtgtcgg ctttcggcga ggacaagttc gaaagcggcc acgcgttcga cggctcgtcg atcgcgggct ggaagggcat cgaggcgtcg gacatgctgc tcatgccgga cccgaacgcc gccttcatcg acccgttcta cgaagagtcg accctcgtgc tgacctgcga cgtcgtcgag cccgccgacg gcaagggcta cgagcgcgac ccgcgctcgc tcgcgaagcg cgccgaggca tacctgaaga gcacgggcat cggcgacacc gcctacttcg gtccggagcc ggaattcttc attttcgact cggtgcagtg gannnncnac atgtcgggct gcttcgtgaa gatcaactcg gaagaagcgc cgtggtcgtc ggcgaaggaa ttcgaangnc ggcaacacgg gccaccgtcc gggcgtgaag ggcggctact tnccctgtcg cgccggtcga ccagttccag gacatgcgct cggaaatgtg cctgctgctc gagcagatcg gcattccggt cgaagtgcac caccacgaag tcgcgggcca gggccagaac gaaatcggca cgaagttctc gacgctcctg cagcgcgcgg actggacgca atggtcgaag tacatcatcc ataacgtcgc gcattcgtac ggcaagacgg cgacgttcat gccgaagccc gtcgtcggcg acaacggctc gggcatgcac gtgcancagt cgatctggaa ggacggccag aacctgttcg cgggcaatgg ctacgccggc ctgtcggaaa cggcgctgtt ctacatcggc ggcatcatca agcacgcgcg cgcgctgaac gcgatcacga acccgacgac gaactcgtac aagcgtctcg tgccgcactt cgaagcgccc gtgaagctcg cttactcggc gcgcaaccgc tcggcgtcga tccgcattcc gcacgtgtcg aacccgaagg gccgccgcat cgaaacgcgc ttcccggacc cgatggcgaa cccgtacctg tgcttctcgg cgctgatgat ggcgggtctc gacggcatcc agaacaagat ccacccgggc gaggctgcgg acaagaacct gtacgacctg ccgccggaag aggatgcaaa gatcccgacc gtgtgcgccg gcctcgacca ggcgctcgag gcgctcgaca aggaccgcga gttcctgacc cgcggcggcg tgttcacgga cgcgatgatc gacgcgtacc tgggcctgaa ggaacaggag ctcgcgaagt tccgcatgac gacgcatccg atcgagttcg agatgtacta ctcgct

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSKTVADVM QLAKDEDVKF VDFRFTDTRG KEQHVSVPVS AFGEDKFESG HAFDGSSIAG WKGIEASDML LMPDPNAAFI DPFYEESTLV LTCDVVEPAD GKGYERDPRS LAKRAEAYLK STGIGDTAYF GPEPEFFIFD SVQWNTDMSG CFVKINSEEA PWSSAKEFEG GNTGHRPGVK GGYFPVAPVD QFQDMRSEMC LLLEQIGIPV EVHHHEVAGQ GQNEIGTKFS TLLQRADWTQ WSKYIIHNVA HSYGKTATFM PKPVVGDNGS GMHVHQSIWK DGQNLFAGNG YAGLSETALF YIGGIIKHAR ALNAITNPTT NSYKRLVPHF EAPVKLAYSA RNRSASIRIP HVSNPKGRRI ETRFPDPMAN PYLCFSALMM AGLDGIQNKI HPGEAADKNL YDLPPEEDAK IPTVCAGLDQ ALEALDKDRE FLTRGGVFTD AMIDAYLGLK EQELAKFRMT THPIEFEMYY SL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtaaaacc gtcgccgacg tcatgcagct cgcgaaggac gaggacgtca agtttgtcga tttccgcttc acggacacgc gcggcaagga gcaacacgtg tcggtgccgg tgtcggcttt cggcgaggac aagttcgaaa gcggccacgc gttcgacggc tcgtcgatcg cgggctggaa gggcatcgag gcgtcggaca tgctgctcat gccggacccg aacgccgcct tcatcgaccc gttctacgaa gagtcgaccc tcgtgctgac ctgcgacgtc gtcgagcccg ccgacggcaa gggctacgag cgcgacccgc gctcgctcgc gaagcgcgcc gaggcatacc tgaagagcac gggcatcggc gacaccgcct acttcggtcc ggagccggaa ttcttcattt tcgactcggt gcagtggaac accgacatgt cgggctgctt cgtgaagatc aactcggaag aagcgccgtg gtcgtcggcg aaggaattcg aaggcggcaa cacgggccac cgtccgggcg tgaagggcgg ctacttccct gtcgcgccgg tcgaccagtt ccaggacatg cgctcggaaa tgtgcctgct gctcgagcag atcggcattc cggtcgaagt gcaccaccac gaagtcgcgg gccagggcca gaacgaaatc ggcacgaagt tctcgacgct cctgcagcgc gcggactgga cgcaatggtc gaagtacatc atccataacg tcgcgcattc gtacggcaag acggcgacgt tcatgccgaa gcccgtcgtc ggcgacaacg gctcgggcat gcacgtgcac cagtcgatct ggaaggacgg ccagaacctg ttcgcgggca atggctacgc cggcctgtcg gaaacggcgc tgttctacat cggcggcatc atcaagcacg cgcgcgcgct gaacgcgatc acgaacccga cgacgaactc gtacaagcgt ctcgtgccgc acttcgaagc gcccgtgaag ctcgcttact cggcgcgcaa ccgctcggcg tcgatccgca ttccgcacgt gtcgaacccg aagggccgcc gcatcgaaac gcgcttcccg gacccgatgg cgaacccgta cctgtgcttc tcggcgctga tgatggcggg tctcgacggc atccagaaca agatccaccc gggcgaggct gcggacaaga acctgtacga cctgccgccg gaagaggatg caaagatccc gaccgtgtgc gccggcctcg accaggcgct cgaggcgctc gacaaggacc gcgagttcct gacccgcggc ggcgtgttca cggacgcgat gatcgacgcg tacctgggcc tgaaggaaca ggagctcgcg aagttccgca tgacgacgca tccgatcgag ttcgagatgt actactcgct gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa

Details for ButhA.00111.a.A1.PW33324

PURIFICATION DATe:	8/12/2011
CONCENTRATION:	33.95mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	100µl
PERCENT IDENTITY:	18
PERCENT COVERAGE:	67

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGTAAAACC GTCGCCGACG TCATGCAGCT CGCGAAGGAC GAGGACGTCA AGTTTGTCGA TTTCCGCTTC ACGGACACGC GCGGCAAGGA GCAACACGTG TCGGTGCCGG TGTCGGCTTT CGGCGAGGAC AAGTTCGAAA GCGGCCACGC GTTCGACGGC TCGTCGATCG CGGGCTGGAA GGGCATCGAG GCGTCGGACA TGCTGCTCAT GCCGGACCCG AACGCCGCCT TCATCGACCC GTTCTACGAA GAGTCGACCC TCGTGCTGAC CTGCGACGTC GTCGAGCCCG CCGACGGCAA GGGCTACGAG CGCGACCCGC GCTCGCTCGC GAAGCGCGCC GAGGCATACC TGAAGAGCAC GGGCATCGGC GACACCGCCT ACTTCGGTCC GGAGCCGGAA TTCTTCATTT TCGACTCGGT GCAGTGGAAC ACCGACATGT CGGGCTGCTT CGTGAAGATC AACTCGGAAG AAGCGCCGTG GTCGTCGGCG AAGGAATTCG AAGGCGGCAA CACGGGCCAC CGTCCGGGCG TGAAGGGCGG CTACTTCCCT GTCGCGCCGG TCGACCAGTT CCAGGACATG CGCTCGGAAA TGTGCCTGCT GCTCGAGCAG ATCGGCATTC CGGTCGAAGT GCACCACCAC GAAGTCGCGG GCCAGGGCCA GAACGAAATC GGCACGAAGT TCTCGACGCT CCTGCAGCGC GCGGACTGGA CGCAATGGTC GAAGTACATC ATCCATAACG TCGCGCATTC GTACGGCAAG ACGGCGACGT TCATGCCGAA GCCCGTCGTC GGCGACAACG GCTCGGGCAT GCACGTGCAC CAGTCGATCT GGAAGGACGG CCAGAACCTG TTCGCGGGCA ATGGCTACGC CGGCCTGTCG GAAACGGCGC TGTTCTACAT CGGCGGCATC ATCAAGCACG CGCGCGCGCT GAACGCGATC ACGAACCCGA CGACGAACTC GTACAAGCGT CTCGTGCCGC ACTTCGAAGC GCCCGTGAAG CTCGCTTACT CGGCGCGCAA CCGCTCGGCG TCGATCCGCA TTCCGCACGT GTCGAACCCG AAGGGCCGCC GCATCGAAAC GCGCTTCCCG GACCCGATGG CGAACCCGTA CCTGTGCTTC TCGGCGCTGA TGATGGCGGG TCTCGACGGC ATCCAGAACA AGATCCACCC GGGCGAGGCT GCGGACAAGA ACCTGTACGA CCTGCCGCCG GAAGAGGATG CAAAGATCCC GACCGTGTGC GCCGGCCTCG ACCAGGCGCT CGAGGCGCTC GACAAGGACC GCGAGTTCCT GACCCGCGGC GGCGTGTTCA CGGACGCGAT GATCGACGCG TACCTGGGCC TGAAGGAACA GGAGCTCGCG AAGTTCCGCA TGACGACGCA TCCGATCGAG TTCGAGATGT ACTACTCGCT GTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA