ButhA.00107.e

Efflux transporter, RND family, MFP subunit

CENTER ID: ButhA.00107.e
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00107.e.A2.GE32416 Mature protein( ButhA.00107.e ) 29 365
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00107.e.A2.PW32336 Mature protein( ButhA.00107.e ) 29 365

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.1428
RefSeq: YP_439484.1
UniProt: Q2T5R5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGRRLLLALA LLAFIFGGLF LWRESRVQAA DHPPAALPAL TVPAVAVRAA PERVTLDAVG SLDAVRQVTL APEVAGRIVA LQFDAGAAVR KGQLIVQLFD EPEHAKLASI QAKAAYAKRQ LDRSQALAQT GAEPRNLFDQ HRFDFDAAAA DIRETQAVIT QKAVRAPFDG VLGLRRVNLG QYLNAGDAIA TLTDLDTLYA NFTVPQKSLS QLRLGQSVSI TTDAYPERTL VARVSAIEPQ ISADTRNVAV QATLSNPDRL LRPGMYIQAR LELPPRERAL TVPDTAVQTS QRGESVLVVG KPGRDGLGVV RLQPVRTGRR ADGRIAIADG LRPGDVVVTS GQVRLAAGDR VKVALDDGAG SGAAQ
NT Sequence
ATGGGCCGTC GGCTATTGCT TGCATTGGCG CTGTTGGCGT TCATCTTCGG CGGGCTGTTC CTGTGGCGGG AAAGCCGCGT GCAGGCCGCC GATCATCCCC CCGCCGCGCT CCCCGCGCTG ACGGTCCCGG CCGTCGCCGT GCGCGCCGCG CCCGAGCGCG TGACGCTCGA CGCGGTCGGC TCGCTCGACG CGGTCCGGCA GGTGACGCTC GCGCCCGAAG TGGCCGGGCG CATCGTCGCG CTGCAGTTCG ACGCGGGCGC GGCGGTGCGC AAGGGGCAAC TGATCGTCCA GCTCTTCGAC GAGCCGGAGC ACGCGAAGCT CGCGTCGATC CAGGCGAAGG CCGCCTACGC GAAGCGCCAG CTCGACCGGT CGCAGGCGCT CGCGCAGACG GGCGCGGAGC CGCGCAACCT CTTCGATCAG CACCGCTTCG ATTTCGACGC GGCCGCGGCC GACATCCGCG AGACGCAGGC GGTGATCACG CAAAAGGCGG TGCGCGCGCC GTTCGACGGC GTGCTCGGCC TGCGCCGCGT GAACCTCGGC CAATATCTGA ACGCGGGCGA CGCGATCGCG ACGCTCACCG ATCTCGACAC GCTGTACGCG AACTTCACGG TGCCGCAGAA GAGCCTGAGC CAGTTGCGTC TCGGCCAGAG CGTATCGATC ACGACGGACG CGTATCCGGA GCGCACGCTC GTCGCGCGCG TGAGCGCGAT CGAGCCGCAG ATCAGCGCCG ACACGCGCAA CGTCGCCGTG CAGGCGACGC TGTCGAACCC CGATCGCCTG CTGCGCCCGG GCATGTACAT CCAGGCGCGG CTCGAGCTGC CGCCGCGCGA GCGCGCGCTG ACCGTGCCCG ACACCGCGGT GCAGACTTCG CAGCGCGGCG AATCGGTGCT CGTCGTCGGC AAGCCGGGCC GCGACGGGCT CGGCGTCGTG CGGCTGCAGC CGGTGAGAAC CGGCCGGCGC GCCGACGGCC GCATCGCGAT CGCAGACGGG CTGCGCCCGG GCGACGTGGT CGTCACGTCG GGCCAGGTAC GGCTCGCGGC GGGCGACAGG GTGAAGGTCG CGCTCGACGA CGGCGCGGGC TCGGGAGCCG CGCAA
Details for ButhA.00107.e.A2.GE32416
HARVESTED ON: 11/8/2011
SEQUENCED ON: 11/11/2011
EXPECTED MW: 38kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MAADHPPAAL PALTVPAVAV RAAPERVTLD AVGSLDAVRQ VTLAPEVAGR IVALQFDAGA AVRKGQLIVQ LFDEPEHAKL ASIQAKAAYA KRQLDRSQAL AQTGAEPRNL FDQHRFDFDA AAADIRETQA VITQKAVRAP FDGVLGLRRV NLGQYLNAGD AIATLTDLDT LYANFTVPQK SLSQLRLGQS VSITTDAYPE RTLVARVSAI EPQISADTRN VAVQATLSNP DRLLRPGMYI QARLELPPRE RALTVPDTAV QTSQRGESVL VVGKPGRDGL GVVRLQPVRT GRRADGRIAI ADGLRPGDVV VTSGQVRLAA GDRVKVALDD GAGSGAAQ
Validated NT Sequence
ttattattgc gcggctcccg agcccgcgcc gtcgtcgagc gcgaccttca ccctgtcgcc cgccgcgagc cgtacctggc ccgacgtgac gaccacgtcg cccgggcgca gcccgtctgc gatcgcgatg cggccgtcgg cgcgccggcc ggttctcacc ggctgcagcc gcacgacgcc gagcccgtcg cggcccggct tgccgacgac gagcaccgat tcgccgcgct gcgaagtctg caccgcggtg tcgggcacgg tcagcgcgcg ctcgcgcggc ggcagctcga gccgcgcctg gatgtacatg cccgggcgca gcaggcgatc ggggttcgac agcgtcgcct gcacggcgac gttgcgcgtg tcggcgctga tctgcggctc gatcgcgctc acgcgcgcga cgagcgtgcg ctccggatac gcgtccgtcg tgatcgatac gctctggccg agacgcaact ggctcaggct cttctgcggc accgtgaagt tcgcgtacag cgtgtcgaga tcggtgagcg tcgcgatcgc gtcgcccgcg ttcagatatt ggccgaggtt cacgcggcgc aggccgagca cgccgtcgaa cggcgcgcgc accgcctttt gcgtgatcac cgcctgcgtc tcgcggatgt cggccgcggc cgcgtcgaaa tcgaagcggt gctgatcgaa gaggttgcgc ggctccgcgc ccgtctgcgc gagcgcctgc gaccggtcga gctggcgctt cgcgtaggcg gccttcgcct ggatcgacgc gagcttcgcg tgctccggct cgtcgaagag ctggacgatc agttgcccct tgcgcaccgc cgcgcccgcg tcgaactgca gcgcgacgat gcgcccggcc acttcgggcg cgagcgtcac ctgccggacc gcgtcgagcg agccgaccgc gtcgagcgtc acgcgctcgg gcgcggcgcg cacggcgacg gccgggaccg tcagcgcggg gagcgcggcg gggggatgat cggcggccat cgaaccagga ccctgggtct gagcttccag gg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAADHPPAA LPALTVPAVA VRAAPERVTL DAVGSLDAVR QVTLAPEVAG RIVALQFDAG AAVRKGQLIV QLFDEPEHAK LASIQAKAAY AKRQLDRSQA LAQTGAEPRN LFDQHRFDFD AAAADIRETQ AVITQKAVRA PFDGVLGLRR VNLGQYLNAG DAIATLTDLD TLYANFTVPQ KSLSQLRLGQ SVSITTDAYP ERTLVARVSA IEPQISADTR NVAVQATLSN PDRLLRPGMY IQARLELPPR ERALTVPDTA VQTSQRGESV LVVGKPGRDG LGVVRLQPVR TGRRADGRIA IADGLRPGDV VVTSGQVRLA AGDRVKVALD DGAGSGAAQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgccgat catccccccg ccgcgctccc cgcgctgacg gtcccggccg tcgccgtgcg cgccgcgccc gagcgcgtga cgctcgacgc ggtcggctcg ctcgacgcgg tccggcaggt gacgctcgcg cccgaagtgg ccgggcgcat cgtcgcgctg cagttcgacg cgggcgcggc ggtgcgcaag gggcaactga tcgtccagct cttcgacgag ccggagcacg cgaagctcgc gtcgatccag gcgaaggccg cctacgcgaa gcgccagctc gaccggtcgc aggcgctcgc gcagacgggc gcggagccgc gcaacctctt cgatcagcac cgcttcgatt tcgacgcggc cgcggccgac atccgcgaga cgcaggcggt gatcacgcaa aaggcggtgc gcgcgccgtt cgacggcgtg ctcggcctgc gccgcgtgaa cctcggccaa tatctgaacg cgggcgacgc gatcgcgacg ctcaccgatc tcgacacgct gtacgcgaac ttcacggtgc cgcagaagag cctgagccag ttgcgtctcg gccagagcgt atcgatcacg acggacgcgt atccggagcg cacgctcgtc gcgcgcgtga gcgcgatcga gccgcagatc agcgccgaca cgcgcaacgt cgccgtgcag gcgacgctgt cgaaccccga tcgcctgctg cgcccgggca tgtacatcca ggcgcggctc gagctgccgc cgcgcgagcg cgcgctgacc gtgcccgaca ccgcggtgca gacttcgcag cgcggcgaat cggtgctcgt cgtcggcaag ccgggccgcg acgggctcgg cgtcgtgcgg ctgcagccgg tgagaaccgg ccggcgcgcc gacggccgca tcgcgatcgc agacgggctg cgcccgggcg acgtggtcgt cacgtcgggc caggtacggc tcgcggcggg cgacagggtg aaggtcgcgc tcgacgacgg cgcgggctcg ggagccgcgc aataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for ButhA.00107.e.A2.PW32336
PURIFICATION DATe: 12/16/2011
CONCENTRATION: 25.69mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MAADHPPAAL PALTVPAVAV RAAPERVTLD AVGSLDAVRQ VTLAPEVAGR IVALQFDAGA AVRKGQLIVQ LFDEPEHAKL ASIQAKAAYA KRQLDRSQAL AQTGAEPRNL FDQHRFDFDA AAADIRETQA VITQKAVRAP FDGVLGLRRV NLGQYLNAGD AIATLTDLDT LYANFTVPQK SLSQLRLGQS VSITTDAYPE RTLVARVSAI EPQISADTRN VAVQATLSNP DRLLRPGMYI QARLELPPRE RALTVPDTAV QTSQRGESVL VVGKPGRDGL GVVRLQPVRT GRRADGRIAI ADGLRPGDVV VTSGQVRLAA GDRVKVALDD GAGSGAAQ
Validated NT Sequence
ttattattgc gcggctcccg agcccgcgcc gtcgtcgagc gcgaccttca ccctgtcgcc cgccgcgagc cgtacctggc ccgacgtgac gaccacgtcg cccgggcgca gcccgtctgc gatcgcgatg cggccgtcgg cgcgccggcc ggttctcacc ggctgcagcc gcacgacgcc gagcccgtcg cggcccggct tgccgacgac gagcaccgat tcgccgcgct gcgaagtctg caccgcggtg tcgggcacgg tcagcgcgcg ctcgcgcggc ggcagctcga gccgcgcctg gatgtacatg cccgggcgca gcaggcgatc ggggttcgac agcgtcgcct gcacggcgac gttgcgcgtg tcggcgctga tctgcggctc gatcgcgctc acgcgcgcga cgagcgtgcg ctccggatac gcgtccgtcg tgatcgatac gctctggccg agacgcaact ggctcaggct cttctgcggc accgtgaagt tcgcgtacag cgtgtcgaga tcggtgagcg tcgcgatcgc gtcgcccgcg ttcagatatt ggccgaggtt cacgcggcgc aggccgagca cgccgtcgaa cggcgcgcgc accgcctttt gcgtgatcac cgcctgcgtc tcgcggatgt cggccgcggc cgcgtcgaaa tcgaagcggt gctgatcgaa gaggttgcgc ggctccgcgc ccgtctgcgc gagcgcctgc gaccggtcga gctggcgctt cgcgtaggcg gccttcgcct ggatcgacgc gagcttcgcg tgctccggct cgtcgaagag ctggacgatc agttgcccct tgcgcaccgc cgcgcccgcg tcgaactgca gcgcgacgat gcgcccggcc acttcgggcg cgagcgtcac ctgccggacc gcgtcgagcg agccgaccgc gtcgagcgtc acgcgctcgg gcgcggcgcg cacggcgacg gccgggaccg tcagcgcggg gagcgcggcg gggggatgat cggcggccat cgaaccagga ccctgggtct gagcttccag gg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAADHPPAA LPALTVPAVA VRAAPERVTL DAVGSLDAVR QVTLAPEVAG RIVALQFDAG AAVRKGQLIV QLFDEPEHAK LASIQAKAAY AKRQLDRSQA LAQTGAEPRN LFDQHRFDFD AAAADIRETQ AVITQKAVRA PFDGVLGLRR VNLGQYLNAG DAIATLTDLD TLYANFTVPQ KSLSQLRLGQ SVSITTDAYP ERTLVARVSA IEPQISADTR NVAVQATLSN PDRLLRPGMY IQARLELPPR ERALTVPDTA VQTSQRGESV LVVGKPGRDG LGVVRLQPVR TGRRADGRIA IADGLRPGDV VVTSGQVRLA AGDRVKVALD DGAGSGAAQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCGCCGAT CATCCCCCCG CCGCGCTCCC CGCGCTGACG GTCCCGGCCG TCGCCGTGCG CGCCGCGCCC GAGCGCGTGA CGCTCGACGC GGTCGGCTCG CTCGACGCGG TCCGGCAGGT GACGCTCGCG CCCGAAGTGG CCGGGCGCAT CGTCGCGCTG CAGTTCGACG CGGGCGCGGC GGTGCGCAAG GGGCAACTGA TCGTCCAGCT CTTCGACGAG CCGGAGCACG CGAAGCTCGC GTCGATCCAG GCGAAGGCCG CCTACGCGAA GCGCCAGCTC GACCGGTCGC AGGCGCTCGC GCAGACGGGC GCGGAGCCGC GCAACCTCTT CGATCAGCAC CGCTTCGATT TCGACGCGGC CGCGGCCGAC ATCCGCGAGA CGCAGGCGGT GATCACGCAA AAGGCGGTGC GCGCGCCGTT CGACGGCGTG CTCGGCCTGC GCCGCGTGAA CCTCGGCCAA TATCTGAACG CGGGCGACGC GATCGCGACG CTCACCGATC TCGACACGCT GTACGCGAAC TTCACGGTGC CGCAGAAGAG CCTGAGCCAG TTGCGTCTCG GCCAGAGCGT ATCGATCACG ACGGACGCGT ATCCGGAGCG CACGCTCGTC GCGCGCGTGA GCGCGATCGA GCCGCAGATC AGCGCCGACA CGCGCAACGT CGCCGTGCAG GCGACGCTGT CGAACCCCGA TCGCCTGCTG CGCCCGGGCA TGTACATCCA GGCGCGGCTC GAGCTGCCGC CGCGCGAGCG CGCGCTGACC GTGCCCGACA CCGCGGTGCA GACTTCGCAG CGCGGCGAAT CGGTGCTCGT CGTCGGCAAG CCGGGCCGCG ACGGGCTCGG CGTCGTGCGG CTGCAGCCGG TGAGAACCGG CCGGCGCGCC GACGGCCGCA TCGCGATCGC AGACGGGCTG CGCCCGGGCG ACGTGGTCGT CACGTCGGGC CAGGTACGGC TCGCGGCGGG CGACAGGGTG AAGGTCGCGC TCGACGACGG CGCGGGCTCG GGAGCCGCGC AATAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA